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The map label for this gene is hisH

Identifier: 57238613

GI number: 57238613

Start: 1669851

End: 1670438

Strand: Direct

Name: hisH

Synonym: CJE1772

Alternate gene names: 57238613

Gene position: 1669851-1670438 (Clockwise)

Preceding gene: 57238612

Following gene: 57238614

Centisome position: 93.93

GC content: 31.46

Gene sequence:

>588_bases
ATGAAAATCATCATCATCGATACAGCCTGTGCGAATTTAGCATCTTTGAAATTTTGTCTAGATAGATTAGGTTTTAATGC
AACTATCAGTAGGGATTTAAAAGAACTTGAAAGTGCGGATAAGCTTTTTTTACCTGGAGTGGGTACCGCAAAAGAAGCAA
TGAAAAATTTAGAACAATTTAATCTTATTGATTTTATTCAAAATACCAAAAAACCTTTGCTTGGTATTTGTTTAGGTATG
CAAATTTTAGGAAATTTTTCTGAAGAATTAAATCAAGAAACCTTAAAACTCATAGATTTTACAACTCAAAAATTTAAAGC
TAAAGAAGGCTTTACTTTTCCACATATGGGATGGAATGAAGTTTATAGCTCTCATGCTTTGTTTAAAGGCTTAGAAGGGG
CTTATTTTTATTTTGTGCATAGTTATTGTGTAGGACTTGGGAAATACACTATAGCAGATTGTGAGTATTCGCAAAAATTT
AGCGCAAGCGTAATGAAAGATAATTTTTACGGAGTGCAATTTCACCCTGAAAGAAGTAGCGAGGCTGGGGAAATTTTAAT
TTCAAATTTTATTAAGGATATAGGATGA

Upstream 100 bases:

>100_bases
ATCATCATAAAGCCGAAGGGCTTTTTAAAGCCTTTGCAAAAGCTTTGAAAATGGCAGTAAAAATAGAAAGTGAAAATTTA
GCCAGCTCTAAAGGAGTGAT

Downstream 100 bases:

>100_bases
CTCAAATCATACCCGCACTCGATCTTATAGATGGCGAAGTGGTGCGTCTTGTAAAAGGGGATTACGAACAAAAAAAAGTA
TATAAATACAATCCCTTAAA

Product: imidazole glycerol phosphate synthase subunit HisH

Products: D-erythro-imidazole-glycerol-phosphate; AICAR; L-glutamate [C]

Alternate protein names: IGP synthase glutamine amidotransferase subunit 2; IGP synthase subunit hisH 2; ImGP synthase subunit hisH 2; IGPS subunit hisH 2

Number of amino acids: Translated: 195; Mature: 195

Protein sequence:

>195_residues
MKIIIIDTACANLASLKFCLDRLGFNATISRDLKELESADKLFLPGVGTAKEAMKNLEQFNLIDFIQNTKKPLLGICLGM
QILGNFSEELNQETLKLIDFTTQKFKAKEGFTFPHMGWNEVYSSHALFKGLEGAYFYFVHSYCVGLGKYTIADCEYSQKF
SASVMKDNFYGVQFHPERSSEAGEILISNFIKDIG

Sequences:

>Translated_195_residues
MKIIIIDTACANLASLKFCLDRLGFNATISRDLKELESADKLFLPGVGTAKEAMKNLEQFNLIDFIQNTKKPLLGICLGM
QILGNFSEELNQETLKLIDFTTQKFKAKEGFTFPHMGWNEVYSSHALFKGLEGAYFYFVHSYCVGLGKYTIADCEYSQKF
SASVMKDNFYGVQFHPERSSEAGEILISNFIKDIG
>Mature_195_residues
MKIIIIDTACANLASLKFCLDRLGFNATISRDLKELESADKLFLPGVGTAKEAMKNLEQFNLIDFIQNTKKPLLGICLGM
QILGNFSEELNQETLKLIDFTTQKFKAKEGFTFPHMGWNEVYSSHALFKGLEGAYFYFVHSYCVGLGKYTIADCEYSQKF
SASVMKDNFYGVQFHPERSSEAGEILISNFIKDIG

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Escherichia coli, GI1788334, Length=196, Percent_Identity=51.530612244898, Blast_Score=202, Evalue=1e-53,
Organism=Saccharomyces cerevisiae, GI6319725, Length=211, Percent_Identity=34.5971563981043, Blast_Score=104, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS52_CAMJE (Q9PM75)

Other databases:

- EMBL:   AL111168
- PIR:   F81255
- RefSeq:   YP_002344969.1
- ProteinModelPortal:   Q9PM75
- SMR:   Q9PM75
- IntAct:   Q9PM75
- MINT:   MINT-6484315
- GeneID:   904385
- GenomeReviews:   AL111168_GR
- KEGG:   cje:Cj1600
- HOGENOM:   HBG292341
- OMA:   YSEEDDI
- PhylomeDB:   Q9PM75
- ProtClustDB:   PRK13141
- BioCyc:   CJEJ192222:CJ1600-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00278
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226
- PIRSF:   PIRSF000495
- TIGRFAMs:   TIGR01855

Pfam domain/function: PF00117 GATase

EC number: 2.4.2.- [C]

Molecular weight: Translated: 21977; Mature: 21977

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 77-77 ACT_SITE 175-175 ACT_SITE 177-177

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIIIIDTACANLASLKFCLDRLGFNATISRDLKELESADKLFLPGVGTAKEAMKNLEQF
CEEEEEEHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHH
NLIDFIQNTKKPLLGICLGMQILGNFSEELNQETLKLIDFTTQKFKAKEGFTFPHMGWNE
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
VYSSHALFKGLEGAYFYFVHSYCVGLGKYTIADCEYSQKFSASVMKDNFYGVQFHPERSS
HHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHCCCEEEEECCCCCC
EAGEILISNFIKDIG
CHHHHHHHHHHHHCC
>Mature Secondary Structure
MKIIIIDTACANLASLKFCLDRLGFNATISRDLKELESADKLFLPGVGTAKEAMKNLEQF
CEEEEEEHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHH
NLIDFIQNTKKPLLGICLGMQILGNFSEELNQETLKLIDFTTQKFKAKEGFTFPHMGWNE
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
VYSSHALFKGLEGAYFYFVHSYCVGLGKYTIADCEYSQKFSASVMKDNFYGVQFHPERSS
HHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHCCCEEEEECCCCCC
EAGEILISNFIKDIG
CHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: phosphoribulosylformimino-AICAR-P; L-glutamine [C]

Specific reaction: phosphoribulosylformimino-AICAR-P + L-glutamine = D-erythro-imidazole-glycerol-phosphate + AICAR + L-glutamate [C]

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10688204