Click here to switch to the map view.

The map label for this gene is metN [H]

Identifier: 57238556

GI number: 57238556

Start: 1609387

End: 1610382

Strand: Reverse

Name: metN [H]

Synonym: CJE1709

Alternate gene names: 57238556

Gene position: 1610382-1609387 (Counterclockwise)

Preceding gene: 57238557

Following gene: 57238555

Centisome position: 90.58

GC content: 23.29

Gene sequence:

>996_bases
ATGATAGAAATTTCTAATCTTTTTTTCAACTATCAAAACAAAGAAGTTTTAAAAATCAAAAATTTAAAACTTGATACAAG
TAAAATTAGCATCTTAATGGGAGCGAATGGAAGTGGAAAATCTACATTTTTAAGAATATTAAAATTTTTAGAAGGAGATT
TTTCAAAAAATATTTCTTATTTTGGAAATTTTAAACCTAATAATAAACAAAAGCGTGAAATTTATTTGCTTTTTCCAGAG
CCTATTTTGTTAAATCGTAGTGTAAGGGCAAATTTTTTATTTACCTTAAAAACTTATGGGATTAAAGAAGATATTGAAGA
ACGCATAAAAGAAAGTTTGATGTTTTTAAATTTAGATGAAAGTCTTTTAAGTAAACATCCTAATGAACTTTCATCAGGAC
AAAGTCAAAAAATAGCTTTTGCTATAGCTTTAAGCGTAAGAGCGAAGTATTATTTACTTGATGAACCAAGTGCTTTTTTG
GATAAAAATACTACACTTTTATTTAAAAAAGCTATTCTTAAAATGCATGAAAATTTCAATACAGGTTTTTTAATCGCAAG
TCATGATAAACATTTTTTAGATTCTTTGGCGCAAAAAAAGCTTTATTTACACAGTGGAGAAATTTTAGAATTTGAAAATA
CTAATGTTTTTGAACTAGAAAATCAAGGTGTGAAATTTTGTAATTTTATAGATTTTAGTAATTGTAAAAAATATAAAGAT
TTTAAAAAACCTCCAAGTAAAATTGCGATTGATCCTTATAAAATTTCTTTTTTTAATTCTAAAAATATCCCAAAAAATAA
TTATGATTTTATTTTAGAAAAATGTTATATTATAGCACTTAGAAGTAGGAAAAGTGATGTTTTTATAAGGGTAAGTTGTA
TGGATAAAATTTTAGAATTTGCCTTAGAAAAACAAGAATTTTTAAAATTCGATTTAAAATTATACGAAGAACTCAGTCTT
TATTTTTATGAAGATGCAATATGTTTCTTAAATTAA

Upstream 100 bases:

>100_bases
AACAAAGGCGAATTTGCTAGCGGTATAGCTTTGGCTTTGGTTTTAATTCTCATTGCCTTTTGTTTAAATTTCATCACGCA
CAAACTCAAAAGAACATAAA

Downstream 100 bases:

>100_bases
CATACATTATAAAATTTAAATAAAATATTACAAATATTCCCATTTTTTATAAAATTTATATTAATTATTTTTTAAATTTG
TATTTAATACAAATAAACTC

Product: tungsten ABC transporter ATP-binding protein

Products: Ni2; [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MIEISNLFFNYQNKEVLKIKNLKLDTSKISILMGANGSGKSTFLRILKFLEGDFSKNISYFGNFKPNNKQKREIYLLFPE
PILLNRSVRANFLFTLKTYGIKEDIEERIKESLMFLNLDESLLSKHPNELSSGQSQKIAFAIALSVRAKYYLLDEPSAFL
DKNTTLLFKKAILKMHENFNTGFLIASHDKHFLDSLAQKKLYLHSGEILEFENTNVFELENQGVKFCNFIDFSNCKKYKD
FKKPPSKIAIDPYKISFFNSKNIPKNNYDFILEKCYIIALRSRKSDVFIRVSCMDKILEFALEKQEFLKFDLKLYEELSL
YFYEDAICFLN

Sequences:

>Translated_331_residues
MIEISNLFFNYQNKEVLKIKNLKLDTSKISILMGANGSGKSTFLRILKFLEGDFSKNISYFGNFKPNNKQKREIYLLFPE
PILLNRSVRANFLFTLKTYGIKEDIEERIKESLMFLNLDESLLSKHPNELSSGQSQKIAFAIALSVRAKYYLLDEPSAFL
DKNTTLLFKKAILKMHENFNTGFLIASHDKHFLDSLAQKKLYLHSGEILEFENTNVFELENQGVKFCNFIDFSNCKKYKD
FKKPPSKIAIDPYKISFFNSKNIPKNNYDFILEKCYIIALRSRKSDVFIRVSCMDKILEFALEKQEFLKFDLKLYEELSL
YFYEDAICFLN
>Mature_331_residues
MIEISNLFFNYQNKEVLKIKNLKLDTSKISILMGANGSGKSTFLRILKFLEGDFSKNISYFGNFKPNNKQKREIYLLFPE
PILLNRSVRANFLFTLKTYGIKEDIEERIKESLMFLNLDESLLSKHPNELSSGQSQKIAFAIALSVRAKYYLLDEPSAFL
DKNTTLLFKKAILKMHENFNTGFLIASHDKHFLDSLAQKKLYLHSGEILEFENTNVFELENQGVKFCNFIDFSNCKKYKD
FKKPPSKIAIDPYKISFFNSKNIPKNNYDFILEKCYIIALRSRKSDVFIRVSCMDKILEFALEKQEFLKFDLKLYEELSL
YFYEDAICFLN

Specific function: Part of the ABC transporter complex MetNIQ involved in methionine import. Responsible for energy coupling to the transport system [H]

COG id: COG3839

COG function: function code G; ABC-type sugar transport systems, ATPase components

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Escherichia coli, GI87082267, Length=198, Percent_Identity=31.8181818181818, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1789891, Length=221, Percent_Identity=30.7692307692308, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI87081791, Length=228, Percent_Identity=28.0701754385965, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI87081709, Length=218, Percent_Identity=27.0642201834862, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788761, Length=214, Percent_Identity=27.5700934579439, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787029, Length=232, Percent_Identity=28.8793103448276, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1790467, Length=219, Percent_Identity=27.8538812785388, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI1788450, Length=219, Percent_Identity=26.027397260274, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1790162, Length=229, Percent_Identity=24.8908296943231, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1788225, Length=226, Percent_Identity=25.6637168141593, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1788165, Length=197, Percent_Identity=27.9187817258883, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1787089, Length=216, Percent_Identity=27.3148148148148, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787500, Length=216, Percent_Identity=24.0740740740741, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786398, Length=225, Percent_Identity=25.7777777777778, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1786563, Length=206, Percent_Identity=26.6990291262136, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1789672, Length=225, Percent_Identity=25.3333333333333, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI145693107, Length=225, Percent_Identity=24.4444444444444, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787370, Length=216, Percent_Identity=23.6111111111111, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788506, Length=196, Percent_Identity=27.5510204081633, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1787712, Length=212, Percent_Identity=25.4716981132075, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1789873, Length=208, Percent_Identity=25.4807692307692, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1787547, Length=234, Percent_Identity=24.3589743589744, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1787164, Length=200, Percent_Identity=27.5, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1789032, Length=311, Percent_Identity=22.508038585209, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1786698, Length=222, Percent_Identity=25.6756756756757, Blast_Score=61, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI212646699, Length=226, Percent_Identity=28.3185840707965, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI193211015, Length=200, Percent_Identity=28.5, Blast_Score=66, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI193211017, Length=200, Percent_Identity=28.5, Blast_Score=66, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI115534520, Length=222, Percent_Identity=25.6756756756757, Blast_Score=65, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6320296, Length=184, Percent_Identity=28.804347826087, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR017908
- InterPro:   IPR003593
- InterPro:   IPR018449 [H]

Pfam domain/function: PF00005 ABC_tran; PF09383 NIL [H]

EC number: NA

Molecular weight: Translated: 38776; Mature: 38776

Theoretical pI: Translated: 9.26; Mature: 9.26

Prosite motif: PS00211 ABC_TRANSPORTER_1 ; PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEISNLFFNYQNKEVLKIKNLKLDTSKISILMGANGSGKSTFLRILKFLEGDFSKNISY
CEEEEEEEEECCCCEEEEEECEEEECCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCE
FGNFKPNNKQKREIYLLFPEPILLNRSVRANFLFTLKTYGIKEDIEERIKESLMFLNLDE
EECCCCCCCCCEEEEEECCCCEEECCCCCCEEEEEEEECCCHHHHHHHHHHHHEEEECCH
SLLSKHPNELSSGQSQKIAFAIALSVRAKYYLLDEPSAFLDKNTTLLFKKAILKMHENFN
HHHHCCCCHHCCCCCCEEEEEEEEEEEEEEEEECCCHHHHCCCCHHHHHHHHHHHHCCCC
TGFLIASHDKHFLDSLAQKKLYLHSGEILEFENTNVFELENQGVKFCNFIDFSNCKKYKD
CEEEEEECCHHHHHHHHHHEEEEECCCEEEECCCCEEEECCCCCEEEEEEECCCCHHHHH
FKKPPSKIAIDPYKISFFNSKNIPKNNYDFILEKCYIIALRSRKSDVFIRVSCMDKILEF
HCCCCCCEEECCEEEEEECCCCCCCCCHHHHHEEEEEEEEECCCCCEEEEHHHHHHHHHH
ALEKQEFLKFDLKLYEELSLYFYEDAICFLN
HHCHHHHHHHHHHHHHHHHEEEEECEEEECC
>Mature Secondary Structure
MIEISNLFFNYQNKEVLKIKNLKLDTSKISILMGANGSGKSTFLRILKFLEGDFSKNISY
CEEEEEEEEECCCCEEEEEECEEEECCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCE
FGNFKPNNKQKREIYLLFPEPILLNRSVRANFLFTLKTYGIKEDIEERIKESLMFLNLDE
EECCCCCCCCCEEEEEECCCCEEECCCCCCEEEEEEEECCCHHHHHHHHHHHHEEEECCH
SLLSKHPNELSSGQSQKIAFAIALSVRAKYYLLDEPSAFLDKNTTLLFKKAILKMHENFN
HHHHCCCCHHCCCCCCEEEEEEEEEEEEEEEEECCCHHHHCCCCHHHHHHHHHHHHCCCC
TGFLIASHDKHFLDSLAQKKLYLHSGEILEFENTNVFELENQGVKFCNFIDFSNCKKYKD
CEEEEEECCHHHHHHHHHHEEEEECCCEEEECCCCEEEECCCCCEEEEEEECCCCHHHHH
FKKPPSKIAIDPYKISFFNSKNIPKNNYDFILEKCYIIALRSRKSDVFIRVSCMDKILEF
HCCCCCCEEECCEEEEEECCCCCCCCCHHHHHEEEEEEEEECCCCCEEEEHHHHHHHHHH
ALEKQEFLKFDLKLYEELSLYFYEDAICFLN
HHCHHHHHHHHHHHHHHHHEEEEECEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Ni2; [Periplasm]; ATP; H2O [C]

Specific reaction: Ni2+ [Periplasm] + ATP + H2O = Ni2+ [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]