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The map label for this gene is fdhB [H]

Identifier: 57238533

GI number: 57238533

Start: 1584271

End: 1584912

Strand: Reverse

Name: fdhB [H]

Synonym: CJE1683

Alternate gene names: 57238533

Gene position: 1584912-1584271 (Counterclockwise)

Preceding gene: 57238534

Following gene: 57238532

Centisome position: 89.15

GC content: 36.45

Gene sequence:

>642_bases
ATGAGTAAGGTAAATTTTGCAAATTTAGAAAAAGAACGCTTGAAATTCTTTTGTGATAATGAGCGTTGTATTGATTGTAA
TGGTTGCGCGGTAGCTTGTGATGAAGCACATGAGCTTCCTATTCATATTCGCCGCCGCCGTGTGATTACTTTAAATGAAG
GAATTCGAGGAAAAGAAGTTTCAACTTCTATTTCTTGTATGCATTGTGATGATGCTCCTTGTTCTATCGTTTGTCCTGTG
GATTGTTTTTACATCCGTGCTGATGGTATTGTTTTACACGATAAAGAAATTTGCATAGGTTGTGGATATTGTCTTTATGC
TTGTCCTTTTGGTGCACCACAATTTCCAAAAGATAGTGTTTTTGGAAATAAAGGCATTATGGATAAATGTACTATGTGTG
CAGGTGGCCCTGAAGCAACGAATTCAGAAAAAGAAAGAGAACTTTATGGTCAAAATCGTATCGCAGAAGGAAAAGTCCCT
GTATGTGCTGCGATGTGTTCAACAAAAGCACTTTTAGTAGGTGAAAGTTCTAAAATAGAAGAGATTTATCATAATAGACT
TATGAATAGAAATTATGGCATTCCAAATCCTTCGGAAAGTTTGGAATGGAAGATTGCTTACACAGGAAAGGAACGCTTAT
GA

Upstream 100 bases:

>100_bases
GCGGAGAAAGTGCTAATACGGTAACAAATTATGGTTATGATATCATGTGTCAGATTCCAGAGACTAAAGGTGGACTTTGT
AGAATCAGTAAGGATGGAAA

Downstream 100 bases:

>100_bases
GAAAAGTATTTGTAACGCTTTTATTAAGCGTAGTGAGTTTATTTGCTTATGGTAGCGAGAGAATGGGACAAGATACTCAA
ATTTGGGATTTTCATCGTAT

Product: formate dehydrogenase, iron-sulfur subunit

Products: CO2; NADH

Alternate protein names: NA

Number of amino acids: Translated: 213; Mature: 212

Protein sequence:

>213_residues
MSKVNFANLEKERLKFFCDNERCIDCNGCAVACDEAHELPIHIRRRRVITLNEGIRGKEVSTSISCMHCDDAPCSIVCPV
DCFYIRADGIVLHDKEICIGCGYCLYACPFGAPQFPKDSVFGNKGIMDKCTMCAGGPEATNSEKERELYGQNRIAEGKVP
VCAAMCSTKALLVGESSKIEEIYHNRLMNRNYGIPNPSESLEWKIAYTGKERL

Sequences:

>Translated_213_residues
MSKVNFANLEKERLKFFCDNERCIDCNGCAVACDEAHELPIHIRRRRVITLNEGIRGKEVSTSISCMHCDDAPCSIVCPV
DCFYIRADGIVLHDKEICIGCGYCLYACPFGAPQFPKDSVFGNKGIMDKCTMCAGGPEATNSEKERELYGQNRIAEGKVP
VCAAMCSTKALLVGESSKIEEIYHNRLMNRNYGIPNPSESLEWKIAYTGKERL
>Mature_212_residues
SKVNFANLEKERLKFFCDNERCIDCNGCAVACDEAHELPIHIRRRRVITLNEGIRGKEVSTSISCMHCDDAPCSIVCPVD
CFYIRADGIVLHDKEICIGCGYCLYACPFGAPQFPKDSVFGNKGIMDKCTMCAGGPEATNSEKERELYGQNRIAEGKVPV
CAAMCSTKALLVGESSKIEEIYHNRLMNRNYGIPNPSESLEWKIAYTGKERL

Specific function: This chain is an electron transfer unit containing 18 cysteine residues, 16 of which occur in four clusters [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787872, Length=180, Percent_Identity=35.5555555555556, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1787122, Length=176, Percent_Identity=34.0909090909091, Blast_Score=100, Evalue=9e-23,
Organism=Escherichia coli, GI226510944, Length=181, Percent_Identity=30.3867403314917, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI2367345, Length=191, Percent_Identity=29.3193717277487, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI1787749, Length=212, Percent_Identity=28.3018867924528, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1789079, Length=208, Percent_Identity=27.8846153846154, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1788811, Length=127, Percent_Identity=32.2834645669291, Blast_Score=71, Evalue=6e-14,
Organism=Escherichia coli, GI1790326, Length=214, Percent_Identity=27.1028037383178, Blast_Score=71, Evalue=6e-14,
Organism=Escherichia coli, GI1789067, Length=178, Percent_Identity=31.4606741573034, Blast_Score=68, Evalue=5e-13,
Organism=Escherichia coli, GI1789370, Length=126, Percent_Identity=32.5396825396825, Blast_Score=67, Evalue=8e-13,
Organism=Escherichia coli, GI87082180, Length=169, Percent_Identity=27.2189349112426, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI2367245, Length=175, Percent_Identity=29.7142857142857, Blast_Score=62, Evalue=2e-11,
Organism=Escherichia coli, GI87082114, Length=90, Percent_Identity=35.5555555555556, Blast_Score=62, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR000813 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: 1.2.1.2

Molecular weight: Translated: 23735; Mature: 23603

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

8.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
11.3 %Cys+Met (Translated Protein)
8.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
10.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKVNFANLEKERLKFFCDNERCIDCNGCAVACDEAHELPIHIRRRRVITLNEGIRGKEV
CCCCCCCCCCHHHHHEEECCCCEEECCCCEEEECCCCCCCEEEEEEEEEEECCCCCCCCC
STSISCMHCDDAPCSIVCPVDCFYIRADGIVLHDKEICIGCGYCLYACPFGAPQFPKDSV
CCEEEEEECCCCCCEEECCCEEEEEEECCEEEECCCEEEECCHHHHCCCCCCCCCCCCCC
FGNKGIMDKCTMCAGGPEATNSEKERELYGQNRIAEGKVPVCAAMCSTKALLVGESSKIE
CCCCCCCHHHHCCCCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHCCEEEEEECCHHHH
EIYHNRLMNRNYGIPNPSESLEWKIAYTGKERL
HHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCC
>Mature Secondary Structure 
SKVNFANLEKERLKFFCDNERCIDCNGCAVACDEAHELPIHIRRRRVITLNEGIRGKEV
CCCCCCCCCHHHHHEEECCCCEEECCCCEEEECCCCCCCEEEEEEEEEEECCCCCCCCC
STSISCMHCDDAPCSIVCPVDCFYIRADGIVLHDKEICIGCGYCLYACPFGAPQFPKDSV
CCEEEEEECCCCCCEEECCCEEEEEEECCEEEECCCEEEECCHHHHCCCCCCCCCCCCCC
FGNKGIMDKCTMCAGGPEATNSEKERELYGQNRIAEGKVPVCAAMCSTKALLVGESSKIE
CCCCCCCHHHHCCCCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHCCEEEEEECCHHHH
EIYHNRLMNRNYGIPNPSESLEWKIAYTGKERL
HHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1781728; 14500908 [H]