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The map label for this gene is tilS [H]

Identifier: 57238476

GI number: 57238476

Start: 1528817

End: 1529782

Strand: Reverse

Name: tilS [H]

Synonym: CJE1626

Alternate gene names: 57238476

Gene position: 1529782-1528817 (Counterclockwise)

Preceding gene: 57238477

Following gene: 57238472

Centisome position: 86.05

GC content: 28.16

Gene sequence:

>966_bases
ATGCAAATTAAAGATGAAATTTTAGCCCTTTTAAAAAAGGGTAAAAATTTACTCGCTTTTTCTTATGGAAGTGATTCTAG
TGCGCTTTTTTATCTTTTAATGCAAGAAAAGATCGACTTTGATCTTGTGATGATAAATTACAAAACAAGAAAAAATAGTG
ATTTAGAAGAGTTAAAAGCCAAAGAACTTGCTTTAAAATTTCATAAAAAAATTTTCATAAAACACGCTCCTAAATTTCAA
AGTAATTTTGAAAAAAAAGCCAGAGATTTTCGCTATGATTTTTTTGAAAAAATTTGTCTTGAGCAAGATTATGATCATCT
TATCTTAGCCCATCATTTAAACGATCAATTTGAATGGTTTTTGATGCAACTTTCACGAGGAGCAGGTTTGGCTGAAATTT
TAGGTATGCAAGAGTGTGAAAAACGCCAAAATTATACACTTTTACGCCCTTTACTCTTTATAAGTAAAGATGAAATTTCA
TCTTTTTTAAAAGAAAAAGATATTTTTTATTTCCATGATGAAAGTAATGAAAATGAAAAATATTTTAGAAATTATATAAG
AAAAAATTTCTCTAATGCCTTTGTGAGTGAATTTCATCAGGGTTTAAAAAGAAGTTTTTCTTATCTTGATGAAGACAGGA
AAAAACTTTATGATTTTGAAAATATCAAAGAAATTCAAGGACTTCTTATTTGCCCTAAAAATGAAAGCTTGATTGCTAGA
GCGGTGAAAATGAAAGGTTTGCTTTTAAGCACGGCACAAAGAAAAGAGCTTTTAAAGGGTGATTGTGTTTTAGGTGGTAA
AATAGCACTTGCATATAAAAACGAACAAGCTATTGTTTTTGAGTATGAAACTTGTCAAAAATTACCTAAAAATTTCAAAG
AAGAGTGTAGAATAGCTAAAATTCCAAGACTTTTAAGAGCTTATTTATACAATCACAAAATCGATATTTCAAGCCTTAGC
TTTTAG

Upstream 100 bases:

>100_bases
GAAAGCGAATGTGGAAATTTAGAAATGGGGCAAATTTATGAATGTGAAATTTTACAAAACCTTGATAAAAAGCTCCTTGC
TAAGGCTTTAAGAAAAGTAG

Downstream 100 bases:

>100_bases
TTTTAAAATTCTTTGATAGCTTAAATGTGTGAGAAAAATCACAAAAAATACTTGCAAAAAAAGTCCTAAAAGTGGTACTG
ATGAGAGTAGATAAAAACAA

Product: PP-loop family protein

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MQIKDEILALLKKGKNLLAFSYGSDSSALFYLLMQEKIDFDLVMINYKTRKNSDLEELKAKELALKFHKKIFIKHAPKFQ
SNFEKKARDFRYDFFEKICLEQDYDHLILAHHLNDQFEWFLMQLSRGAGLAEILGMQECEKRQNYTLLRPLLFISKDEIS
SFLKEKDIFYFHDESNENEKYFRNYIRKNFSNAFVSEFHQGLKRSFSYLDEDRKKLYDFENIKEIQGLLICPKNESLIAR
AVKMKGLLLSTAQRKELLKGDCVLGGKIALAYKNEQAIVFEYETCQKLPKNFKEECRIAKIPRLLRAYLYNHKIDISSLS
F

Sequences:

>Translated_321_residues
MQIKDEILALLKKGKNLLAFSYGSDSSALFYLLMQEKIDFDLVMINYKTRKNSDLEELKAKELALKFHKKIFIKHAPKFQ
SNFEKKARDFRYDFFEKICLEQDYDHLILAHHLNDQFEWFLMQLSRGAGLAEILGMQECEKRQNYTLLRPLLFISKDEIS
SFLKEKDIFYFHDESNENEKYFRNYIRKNFSNAFVSEFHQGLKRSFSYLDEDRKKLYDFENIKEIQGLLICPKNESLIAR
AVKMKGLLLSTAQRKELLKGDCVLGGKIALAYKNEQAIVFEYETCQKLPKNFKEECRIAKIPRLLRAYLYNHKIDISSLS
F
>Mature_321_residues
MQIKDEILALLKKGKNLLAFSYGSDSSALFYLLMQEKIDFDLVMINYKTRKNSDLEELKAKELALKFHKKIFIKHAPKFQ
SNFEKKARDFRYDFFEKICLEQDYDHLILAHHLNDQFEWFLMQLSRGAGLAEILGMQECEKRQNYTLLRPLLFISKDEIS
SFLKEKDIFYFHDESNENEKYFRNYIRKNFSNAFVSEFHQGLKRSFSYLDEDRKKLYDFENIKEIQGLLICPKNESLIAR
AVKMKGLLLSTAQRKELLKGDCVLGGKIALAYKNEQAIVFEYETCQKLPKNFKEECRIAKIPRLLRAYLYNHKIDISSLS
F

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=185, Percent_Identity=28.6486486486486, Blast_Score=92, Evalue=6e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012795
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3 [H]

EC number: NA

Molecular weight: Translated: 38042; Mature: 38042

Theoretical pI: Translated: 9.14; Mature: 9.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIKDEILALLKKGKNLLAFSYGSDSSALFYLLMQEKIDFDLVMINYKTRKNSDLEELKA
CCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHH
KELALKFHKKIFIKHAPKFQSNFEKKARDFRYDFFEKICLEQDYDHLILAHHLNDQFEWF
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHCCHHHHHH
LMQLSRGAGLAEILGMQECEKRQNYTLLRPLLFISKDEISSFLKEKDIFYFHDESNENEK
HHHHHCCCCHHHHHCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCHH
YFRNYIRKNFSNAFVSEFHQGLKRSFSYLDEDRKKLYDFENIKEIQGLLICPKNESLIAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCEEECCCCHHHHHH
AVKMKGLLLSTAQRKELLKGDCVLGGKIALAYKNEQAIVFEYETCQKLPKNFKEECRIAK
HHHHHHHHHHHHHHHHHHCCCCEECCEEEEEEECCCEEEEEHHHHHHHHHHHHHHHHHHH
IPRLLRAYLYNHKIDISSLSF
HHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MQIKDEILALLKKGKNLLAFSYGSDSSALFYLLMQEKIDFDLVMINYKTRKNSDLEELKA
CCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHH
KELALKFHKKIFIKHAPKFQSNFEKKARDFRYDFFEKICLEQDYDHLILAHHLNDQFEWF
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHCCHHHHHH
LMQLSRGAGLAEILGMQECEKRQNYTLLRPLLFISKDEISSFLKEKDIFYFHDESNENEK
HHHHHCCCCHHHHHCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCHH
YFRNYIRKNFSNAFVSEFHQGLKRSFSYLDEDRKKLYDFENIKEIQGLLICPKNESLIAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCEEECCCCHHHHHH
AVKMKGLLLSTAQRKELLKGDCVLGGKIALAYKNEQAIVFEYETCQKLPKNFKEECRIAK
HHHHHHHHHHHHHHHHHHCCCCEECCEEEEEEECCCEEEEEHHHHHHHHHHHHHHHHHHH
IPRLLRAYLYNHKIDISSLSF
HHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10688204 [H]