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The map label for this gene is selB [H]

Identifier: 57238422

GI number: 57238422

Start: 1472186

End: 1473991

Strand: Direct

Name: selB [H]

Synonym: CJE1570

Alternate gene names: 57238422

Gene position: 1472186-1473991 (Clockwise)

Preceding gene: 57238421

Following gene: 57238423

Centisome position: 82.81

GC content: 25.86

Gene sequence:

>1806_bases
ATGAAGTCTGTTATTATAGGAACTGCAGGACATATTGATCATGGAAAAACTTCACTCATTAAAGCTTTAAATGGTTTTGA
AGGTGATAGTTTAAAAGAAGAACAAGAAAGACAAATCACCATTAATTTAAGTTTTTCAAATCTTAAATTAAAAGATAAAA
ACATTTCTTTTATCGATGTTCCAGGACATAAAGATTTGGTTAAAACTATGGTAAGTGGAGCTTTTGGCTTTAGTATTTGT
TTGTTTGTTGTAGATATTAACGAAGGTTTAAAAGAACAAAGTTTAGAACATTTAGAAATTTTAAAAATTCTTGATATTAA
AAATATCATTTTAGTGCTTAGCAAATGCGATCTTTGTGAAAATATAGAGCAAAAAAGTGTAGAAATTTTAGAAGAGTTAA
AAAATTTAGATTATCCCATTTTAAAAGTATTTCACACCAGTATAAAAAATAATCAAGGCATAGAAGAATTAAAAAATTAT
CTTTATACCATAGAAAACAAAGAAAATGATGAAGAACTGATTTTTCATTATTATATCGATAGAGTTTTTTCTCTAAAAGG
CATAGGAACTGTCGTCACAGGCAGCTTGAATGAAGGAAGTATCACTTTAAATGAAAAAATCATCTGTTTAGACACTCAAA
AAGAACTCATTGTTAAAAATATACAAAATCACGATACTAATTTAGAGCAAATCAAAGCTTGTAACCGTGTGGCTTTGAGT
TTAAATTGTGATTATAAAGAGTTAAAAAAAGGTTATTTGCTAAGTAAAAAAGGTTATTTTAAAGCCTTTAAAGAATGCGA
TGCTTTAGTTAAAGCAAAAAATTTACAAAATAACAAAATGATTTTTTGCGTAGGTTCAAGACAAATAGAATGCAAAATAA
ATATATTAAAAAAACTAGAAAATGATGAATTTTTTGTGCATTTTAGTTTTGATAAAAATGTATTTTTAAGTTTTGATGAG
GCTTTTATTTTATTACAAAACAATCGTGTTATAGGCGGTGGTAAGGTTTTAAATCCTTTAAGCGAACCTTTAAAAAAAGA
ACAAAAAAATAAATTTTTAATGTTTTTGAAAAATAAAGATTTTAAAGCGGCTTTTTCTTTCTTAAAAGACGCACACAAAT
ACGGCTTTGGCTTGCTTTCAAGTTATCAGAGATTTAAACTTTCTCATCAAAAAGCATTGAAATTAGCAAAAGAATTAAAT
CAAGTATTTGTTGATGAAAAAAATCTTAATGTATATCATTTACAAAGTCTAGAAGAAATTAAAAATTTCATCAAATTCAT
ACTTGAAAAAAATCCTTATGCCATGCTTTCAGCACATTCTTTAGCTTTAAGGATTACTTGGGCAAGTGAAAATTTTTGCG
AACTCGGGCTTAAAGAGATGTCAAATTTATTGGATTTTCAAAATGGAATTTATTTTAAAAAAGGCATAGATTTTGAAAAA
CTTCAAGAAAAAAATAATAATCAATTATATGAAATTTTAAAAAAACAAGGTATTAAACCCGAAGCTCCTTATAATCTTTA
TGATTTTTTAGAACTTGATAGAAAAAGCGGAGATAATATACTCAAAAAACTTACCCAAAAAGGCTTGGTTGTAAGACTTT
CACACAATCTTTTTATAGAAAAACAAGCTCTTGAAAAACTTATGCAAGAATGTTTAAATTTATTAAAAAATCAAAGTCTT
GATGTGCAAAGTATGAAAGAATATTTCAATCTTTCAAGAAAATATGCTATTGCTTATTTAGAATACTTAGACAAATTTCC
TCAAGTAAGCAAAGAAGCTGAAAAAAGATTTTTAACAAACATTTAG

Upstream 100 bases:

>100_bases
TAGGACGCATTGAAAATGATGAATTTGTTCTTGATTTTAGAACTATACGAGAAAATGAACTTCAAAAACTTATTCTTACA
ATCAATCAAATGGAAAACTT

Downstream 100 bases:

>100_bases
AAAATCATTATAGAATTAAACATAATTTATTTAAAGGATTTATAAACTATGAAAAAATTAAGTTTAATTCTTGCCTGTTC
GGCATCTTTGTTTGCTGCAA

Product: selenocysteine-specific elongation factor

Products: NA

Alternate protein names: SelB translation factor [H]

Number of amino acids: Translated: 601; Mature: 601

Protein sequence:

>601_residues
MKSVIIGTAGHIDHGKTSLIKALNGFEGDSLKEEQERQITINLSFSNLKLKDKNISFIDVPGHKDLVKTMVSGAFGFSIC
LFVVDINEGLKEQSLEHLEILKILDIKNIILVLSKCDLCENIEQKSVEILEELKNLDYPILKVFHTSIKNNQGIEELKNY
LYTIENKENDEELIFHYYIDRVFSLKGIGTVVTGSLNEGSITLNEKIICLDTQKELIVKNIQNHDTNLEQIKACNRVALS
LNCDYKELKKGYLLSKKGYFKAFKECDALVKAKNLQNNKMIFCVGSRQIECKINILKKLENDEFFVHFSFDKNVFLSFDE
AFILLQNNRVIGGGKVLNPLSEPLKKEQKNKFLMFLKNKDFKAAFSFLKDAHKYGFGLLSSYQRFKLSHQKALKLAKELN
QVFVDEKNLNVYHLQSLEEIKNFIKFILEKNPYAMLSAHSLALRITWASENFCELGLKEMSNLLDFQNGIYFKKGIDFEK
LQEKNNNQLYEILKKQGIKPEAPYNLYDFLELDRKSGDNILKKLTQKGLVVRLSHNLFIEKQALEKLMQECLNLLKNQSL
DVQSMKEYFNLSRKYAIAYLEYLDKFPQVSKEAEKRFLTNI

Sequences:

>Translated_601_residues
MKSVIIGTAGHIDHGKTSLIKALNGFEGDSLKEEQERQITINLSFSNLKLKDKNISFIDVPGHKDLVKTMVSGAFGFSIC
LFVVDINEGLKEQSLEHLEILKILDIKNIILVLSKCDLCENIEQKSVEILEELKNLDYPILKVFHTSIKNNQGIEELKNY
LYTIENKENDEELIFHYYIDRVFSLKGIGTVVTGSLNEGSITLNEKIICLDTQKELIVKNIQNHDTNLEQIKACNRVALS
LNCDYKELKKGYLLSKKGYFKAFKECDALVKAKNLQNNKMIFCVGSRQIECKINILKKLENDEFFVHFSFDKNVFLSFDE
AFILLQNNRVIGGGKVLNPLSEPLKKEQKNKFLMFLKNKDFKAAFSFLKDAHKYGFGLLSSYQRFKLSHQKALKLAKELN
QVFVDEKNLNVYHLQSLEEIKNFIKFILEKNPYAMLSAHSLALRITWASENFCELGLKEMSNLLDFQNGIYFKKGIDFEK
LQEKNNNQLYEILKKQGIKPEAPYNLYDFLELDRKSGDNILKKLTQKGLVVRLSHNLFIEKQALEKLMQECLNLLKNQSL
DVQSMKEYFNLSRKYAIAYLEYLDKFPQVSKEAEKRFLTNI
>Mature_601_residues
MKSVIIGTAGHIDHGKTSLIKALNGFEGDSLKEEQERQITINLSFSNLKLKDKNISFIDVPGHKDLVKTMVSGAFGFSIC
LFVVDINEGLKEQSLEHLEILKILDIKNIILVLSKCDLCENIEQKSVEILEELKNLDYPILKVFHTSIKNNQGIEELKNY
LYTIENKENDEELIFHYYIDRVFSLKGIGTVVTGSLNEGSITLNEKIICLDTQKELIVKNIQNHDTNLEQIKACNRVALS
LNCDYKELKKGYLLSKKGYFKAFKECDALVKAKNLQNNKMIFCVGSRQIECKINILKKLENDEFFVHFSFDKNVFLSFDE
AFILLQNNRVIGGGKVLNPLSEPLKKEQKNKFLMFLKNKDFKAAFSFLKDAHKYGFGLLSSYQRFKLSHQKALKLAKELN
QVFVDEKNLNVYHLQSLEEIKNFIKFILEKNPYAMLSAHSLALRITWASENFCELGLKEMSNLLDFQNGIYFKKGIDFEK
LQEKNNNQLYEILKKQGIKPEAPYNLYDFLELDRKSGDNILKKLTQKGLVVRLSHNLFIEKQALEKLMQECLNLLKNQSL
DVQSMKEYFNLSRKYAIAYLEYLDKFPQVSKEAEKRFLTNI

Specific function: Translation factor necessary for the incorporation of selenocysteine into proteins. It probably replaces EF-Tu for the insertion of selenocysteine directed by the UGA codon. SelB binds GTP and GDP [H]

COG id: COG3276

COG function: function code J; Selenocysteine-specific translation elongation factor

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. SelB subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=392, Percent_Identity=25.2551020408163, Blast_Score=115, Evalue=9e-26,
Organism=Homo sapiens, GI54607086, Length=248, Percent_Identity=29.4354838709677, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI5729864, Length=288, Percent_Identity=23.6111111111111, Blast_Score=79, Evalue=2e-14,
Organism=Homo sapiens, GI223555963, Length=288, Percent_Identity=23.6111111111111, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI2367247, Length=368, Percent_Identity=26.3586956521739, Blast_Score=159, Evalue=7e-40,
Organism=Escherichia coli, GI1790412, Length=380, Percent_Identity=25.5263157894737, Blast_Score=107, Evalue=3e-24,
Organism=Escherichia coli, GI1789737, Length=375, Percent_Identity=25.3333333333333, Blast_Score=105, Evalue=8e-24,
Organism=Escherichia coli, GI1789108, Length=300, Percent_Identity=22.3333333333333, Blast_Score=63, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17506081, Length=350, Percent_Identity=26.8571428571429, Blast_Score=111, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17556456, Length=408, Percent_Identity=24.0196078431373, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI25141371, Length=281, Percent_Identity=28.8256227758007, Blast_Score=101, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6324761, Length=292, Percent_Identity=27.0547945205479, Blast_Score=97, Evalue=6e-21,
Organism=Drosophila melanogaster, GI281363316, Length=408, Percent_Identity=24.5098039215686, Blast_Score=103, Evalue=4e-22,
Organism=Drosophila melanogaster, GI17864358, Length=408, Percent_Identity=24.5098039215686, Blast_Score=103, Evalue=4e-22,
Organism=Drosophila melanogaster, GI19922690, Length=284, Percent_Identity=29.5774647887324, Blast_Score=102, Evalue=9e-22,
Organism=Drosophila melanogaster, GI19921738, Length=287, Percent_Identity=28.2229965156794, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI45553807, Length=269, Percent_Identity=24.5353159851301, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI45553816, Length=269, Percent_Identity=24.5353159851301, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24651721, Length=269, Percent_Identity=24.5353159851301, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI17864154, Length=269, Percent_Identity=24.5353159851301, Blast_Score=69, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015189
- InterPro:   IPR015190
- InterPro:   IPR015191
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR009000
- InterPro:   IPR004535
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF09105 SelB-wing_1; PF09106 SelB-wing_2; PF09107 SelB-wing_3 [H]

EC number: NA

Molecular weight: Translated: 69504; Mature: 69504

Theoretical pI: Translated: 8.64; Mature: 8.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSVIIGTAGHIDHGKTSLIKALNGFEGDSLKEEQERQITINLSFSNLKLKDKNISFIDV
CCCEEEECCCCCCCCHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEECEEEECCCEEEEEC
PGHKDLVKTMVSGAFGFSICLFVVDINEGLKEQSLEHLEILKILDIKNIILVLSKCDLCE
CCCHHHHHHHHHCCHHHEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHH
NIEQKSVEILEELKNLDYPILKVFHTSIKNNQGIEELKNYLYTIENKENDEELIFHYYID
HHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHEEECCCCCCHHHHHHHHHH
RVFSLKGIGTVVTGSLNEGSITLNEKIICLDTQKELIVKNIQNHDTNLEQIKACNRVALS
HHHHHCCCCEEEECCCCCCEEEECCEEEEEECHHHHHHHHHHCCCCCHHHHHHHCCEEEE
LNCDYKELKKGYLLSKKGYFKAFKECDALVKAKNLQNNKMIFCVGSRQIECKINILKKLE
ECCCHHHHHCCCEECCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEEEEHHHHHCC
NDEFFVHFSFDKNVFLSFDEAFILLQNNRVIGGGKVLNPLSEPLKKEQKNKFLMFLKNKD
CCCEEEEEEECCCEEEEECCEEEEEECCEEECCCHHHHHHHHHHHHHCCCCEEEEEECCC
FKAAFSFLKDAHKYGFGLLSSYQRFKLSHQKALKLAKELNQVFVDEKNLNVYHLQSLEEI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHH
KNFIKFILEKNPYAMLSAHSLALRITWASENFCELGLKEMSNLLDFQNGIYFKKGIDFEK
HHHHHHHHCCCCCEEEEECEEEEEEEECCCCHHHHHHHHHHHHHHHHCCEEEECCCCHHH
LQEKNNNQLYEILKKQGIKPEAPYNLYDFLELDRKSGDNILKKLTQKGLVVRLSHNLFIE
HHCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHCCEEEEEECCEEEE
KQALEKLMQECLNLLKNQSLDVQSMKEYFNLSRKYAIAYLEYLDKFPQVSKEAEKRFLTN
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHCHHHHHHHHHHHHHHHCHHHHHHHHHHHHCC
I
C
>Mature Secondary Structure
MKSVIIGTAGHIDHGKTSLIKALNGFEGDSLKEEQERQITINLSFSNLKLKDKNISFIDV
CCCEEEECCCCCCCCHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEECEEEECCCEEEEEC
PGHKDLVKTMVSGAFGFSICLFVVDINEGLKEQSLEHLEILKILDIKNIILVLSKCDLCE
CCCHHHHHHHHHCCHHHEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHH
NIEQKSVEILEELKNLDYPILKVFHTSIKNNQGIEELKNYLYTIENKENDEELIFHYYID
HHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHEEECCCCCCHHHHHHHHHH
RVFSLKGIGTVVTGSLNEGSITLNEKIICLDTQKELIVKNIQNHDTNLEQIKACNRVALS
HHHHHCCCCEEEECCCCCCEEEECCEEEEEECHHHHHHHHHHCCCCCHHHHHHHCCEEEE
LNCDYKELKKGYLLSKKGYFKAFKECDALVKAKNLQNNKMIFCVGSRQIECKINILKKLE
ECCCHHHHHCCCEECCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEEEEHHHHHCC
NDEFFVHFSFDKNVFLSFDEAFILLQNNRVIGGGKVLNPLSEPLKKEQKNKFLMFLKNKD
CCCEEEEEEECCCEEEEECCEEEEEECCEEECCCHHHHHHHHHHHHHCCCCEEEEEECCC
FKAAFSFLKDAHKYGFGLLSSYQRFKLSHQKALKLAKELNQVFVDEKNLNVYHLQSLEEI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHH
KNFIKFILEKNPYAMLSAHSLALRITWASENFCELGLKEMSNLLDFQNGIYFKKGIDFEK
HHHHHHHHCCCCCEEEEECEEEEEEEECCCCHHHHHHHHHHHHHHHHCCEEEECCCCHHH
LQEKNNNQLYEILKKQGIKPEAPYNLYDFLELDRKSGDNILKKLTQKGLVVRLSHNLFIE
HHCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHCCEEEEEECCEEEE
KQALEKLMQECLNLLKNQSLDVQSMKEYFNLSRKYAIAYLEYLDKFPQVSKEAEKRFLTN
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHCHHHHHHHHHHHHHHHCHHHHHHHHHHHHCC
I
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8893853 [H]