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The map label for this gene is pldA [C]

Identifier: 57238394

GI number: 57238394

Start: 1435658

End: 1436647

Strand: Direct

Name: pldA [C]

Synonym: CJE1540

Alternate gene names: 57238394

Gene position: 1435658-1436647 (Clockwise)

Preceding gene: 57238393

Following gene: 57238395

Centisome position: 80.75

GC content: 28.28

Gene sequence:

>990_bases
ATGAGAAAAATTGCTTTATTTTTAAGCTTATGCGTTTTTATATGGGCTAGTGATTTACAACAAGCTTTAGAGTATGAAAA
ACAAGGCGATTATAAAAAAGCTATGGAAATTTACAAAAAACTCGCTTTAAAAAATTCATCTGTTTTGATTTCTCAAGAAC
AAAATAACTCAAGCGAAGCAACACAAATGCAAAATTCTATCACTATAAAAAAAGAAGAAAAACAAGACTTTTCTCGCTTA
GCTTTGGCTAATTATCTTGGTGAAAATGAAAGTTTTAATCCCCTTGGCATTAGCTCTTATAAGATGAATTATTTTTTACC
TTTTGCTTATAGTTTTAATTCTTTAGGAACAAATAATAATAAGAGTGAGGCGAAATTTCAACTTAGTGTTAAAAAAAGAC
TTTTTGAAAATTTACTAGGACTAGATGAAAAATATTATATAGCCTATACTCAAACTTCTTGGTGGCAAATTTATAAGCAT
TCTTCTCCTTTTAGAGAAACCAACTATCAACCAGAATTTTTTATAGATCTTCCTTTATATCTTAAAGATTATGAATTTTT
TAACAATCTACGCGTAGGTATATTGCATGAAAGCAATGGCAAGGGAGATGAGAATTTACAATCTCGCTCATGGAATAGAA
TTTATGTATCTACTGCTATTTTATATAATAAATTTTTATTTGTTCCACGCCTTTGGTATAGAATTCCTGAAAACAAAAAA
GATGATGATAATCCTGCCATCTTGCATTATATGGGAAATTTTGATGTGAATTTAGCGTATTTAGGTGATGATTATTTTAT
CAATCTTATGCTAAGAAATAATCTTAAATTCCACAATAATAAAGGCGCTATACAAGTTGATCTAGGATATGATATTTTTA
ATAACGGAATTTATTGGTATTTACAATACTTTAATGGCTATGGAGAAAGCCTTATAGACTATAACAAACACTTACAAAGA
CTTTCCACTGGATTTTTGATTTCTTACTAA

Upstream 100 bases:

>100_bases
TTTCTGAGATTAAAACCAAATTTGTAGAATTTGAAGATGAAGATGCTTTTTTAAATCTTAATTTTTATGAAGAATATGAA
AAATTTAAAAGCAAGTTAAA

Downstream 100 bases:

>100_bases
AATATCCTTTGTAGTTTTATCATTTCTTAAAACAATTTTCATTACAATTTCATTTTTGATAATTATTATTACTATAAGGA
AAGATTTGTTTTTTAAGCAT

Product: phospholipase A

Products: NA

Alternate protein names: Phosphatidylcholine 1-acylhydrolase [H]

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MRKIALFLSLCVFIWASDLQQALEYEKQGDYKKAMEIYKKLALKNSSVLISQEQNNSSEATQMQNSITIKKEEKQDFSRL
ALANYLGENESFNPLGISSYKMNYFLPFAYSFNSLGTNNNKSEAKFQLSVKKRLFENLLGLDEKYYIAYTQTSWWQIYKH
SSPFRETNYQPEFFIDLPLYLKDYEFFNNLRVGILHESNGKGDENLQSRSWNRIYVSTAILYNKFLFVPRLWYRIPENKK
DDDNPAILHYMGNFDVNLAYLGDDYFINLMLRNNLKFHNNKGAIQVDLGYDIFNNGIYWYLQYFNGYGESLIDYNKHLQR
LSTGFLISY

Sequences:

>Translated_329_residues
MRKIALFLSLCVFIWASDLQQALEYEKQGDYKKAMEIYKKLALKNSSVLISQEQNNSSEATQMQNSITIKKEEKQDFSRL
ALANYLGENESFNPLGISSYKMNYFLPFAYSFNSLGTNNNKSEAKFQLSVKKRLFENLLGLDEKYYIAYTQTSWWQIYKH
SSPFRETNYQPEFFIDLPLYLKDYEFFNNLRVGILHESNGKGDENLQSRSWNRIYVSTAILYNKFLFVPRLWYRIPENKK
DDDNPAILHYMGNFDVNLAYLGDDYFINLMLRNNLKFHNNKGAIQVDLGYDIFNNGIYWYLQYFNGYGESLIDYNKHLQR
LSTGFLISY
>Mature_329_residues
MRKIALFLSLCVFIWASDLQQALEYEKQGDYKKAMEIYKKLALKNSSVLISQEQNNSSEATQMQNSITIKKEEKQDFSRL
ALANYLGENESFNPLGISSYKMNYFLPFAYSFNSLGTNNNKSEAKFQLSVKKRLFENLLGLDEKYYIAYTQTSWWQIYKH
SSPFRETNYQPEFFIDLPLYLKDYEFFNNLRVGILHESNGKGDENLQSRSWNRIYVSTAILYNKFLFVPRLWYRIPENKK
DDDNPAILHYMGNFDVNLAYLGDDYFINLMLRNNLKFHNNKGAIQVDLGYDIFNNGIYWYLQYFNGYGESLIDYNKHLQR
LSTGFLISY

Specific function: Hydrolysis of phosphatidylcholine with phospholipase A2 (EC 3.1.1.4) and phospholipase A1 (EC 3.1.1.32) activities [H]

COG id: COG2829

COG function: function code M; Outer membrane phospholipase A

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein. Note=One of the very few enzymes located there (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phospholipase A1 family [H]

Homologues:

Organism=Escherichia coli, GI2367300, Length=261, Percent_Identity=34.0996168582375, Blast_Score=125, Evalue=3e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003187 [H]

Pfam domain/function: PF02253 PLA1 [H]

EC number: =3.1.1.32; =3.1.1.4 [H]

Molecular weight: Translated: 38912; Mature: 38912

Theoretical pI: Translated: 8.24; Mature: 8.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKIALFLSLCVFIWASDLQQALEYEKQGDYKKAMEIYKKLALKNSSVLISQEQNNSSEA
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEECCCCCHHH
TQMQNSITIKKEEKQDFSRLALANYLGENESFNPLGISSYKMNYFLPFAYSFNSLGTNNN
HHHHHCEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEEEECCCCCCCCC
KSEAKFQLSVKKRLFENLLGLDEKYYIAYTQTSWWQIYKHSSPFRETNYQPEFFIDLPLY
CCCEEEEHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEECCCCCHHCCCCCEEEEECCHH
LKDYEFFNNLRVGILHESNGKGDENLQSRSWNRIYVSTAILYNKFLFVPRLWYRIPENKK
HHHHHHHCCCEEEEEECCCCCCCCCHHHCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCC
DDDNPAILHYMGNFDVNLAYLGDDYFINLMLRNNLKFHNNKGAIQVDLGYDIFNNGIYWY
CCCCCEEEEEECCCEEEEEEECCCEEEEEEEECCCEEECCCCEEEEEECCEECCCCEEEE
LQYFNGYGESLIDYNKHLQRLSTGFLISY
EEEHHCCCHHHHHHHHHHHHHHCCCEEEC
>Mature Secondary Structure
MRKIALFLSLCVFIWASDLQQALEYEKQGDYKKAMEIYKKLALKNSSVLISQEQNNSSEA
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEECCCCCHHH
TQMQNSITIKKEEKQDFSRLALANYLGENESFNPLGISSYKMNYFLPFAYSFNSLGTNNN
HHHHHCEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEEEECCCCCCCCC
KSEAKFQLSVKKRLFENLLGLDEKYYIAYTQTSWWQIYKHSSPFRETNYQPEFFIDLPLY
CCCEEEEHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEECCCCCHHCCCCCEEEEECCHH
LKDYEFFNNLRVGILHESNGKGDENLQSRSWNRIYVSTAILYNKFLFVPRLWYRIPENKK
HHHHHHHCCCEEEEEECCCCCCCCCHHHCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCC
DDDNPAILHYMGNFDVNLAYLGDDYFINLMLRNNLKFHNNKGAIQVDLGYDIFNNGIYWY
CCCCCEEEEEECCCEEEEEEECCCEEEEEEEECCCEEECCCCEEEEEECCEECCCCEEEE
LQYFNGYGESLIDYNKHLQRLSTGFLISY
EEEHHCCCHHHHHHHHHHHHHHCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10710307 [H]