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The map label for this gene is bacA [H]

Identifier: 57238354

GI number: 57238354

Start: 1391138

End: 1392646

Strand: Direct

Name: bacA [H]

Synonym: CJE1496

Alternate gene names: 57238354

Gene position: 1391138-1392646 (Clockwise)

Preceding gene: 57238351

Following gene: 57238355

Centisome position: 78.25

GC content: 29.03

Gene sequence:

>1509_bases
ATGACAACTCATATCTATGATTTTTTAGAAAAAAGTTTAATCAAATTTAGCGAAAAAACTGCTTTTGTAGAACCCTTTGC
CAAAGAAAGAAAGGAAATCACTTACAAAGACTTTGATCTTTTTTCTAAGAAACTAGCCAGTGAAATTTTAAAAACTTTAA
AAAATAATAAACCTACTCAAGCTCCTGTTTTAATCATCTTGCCTAAAGGGATTGATTGTTTGATTTCATTTTTTGGAGTG
GCACTCAGTGGAAATTTTTATACACTTTTAGATGAAAAAAGTCCTAAAGAAAGAGTAGAAAAAGTCATAGAAGTTTTAAA
GCCTAAACTTTTTATCACTTCAAAGGACTTAAATTTTAACTTAGATTTACCTACGCTTTATACAGAAGATTTTGAAAGTT
TTAATATAGATGAGAGTTTAATAAAGCATGCCAAAGAAAAACATATAGATACTAATTTACTCTATGTTTTCTTTACAAGT
GGGAGCACAGGCATTCCTAAAGGAGTAAGCATAGCTCATAAGAGTGTGATTGATTATACTTTTTGGGTGTGTGAGACTTT
TAAATTTGATGAGAATGAAATTTTAGCCAATCAAGCGCCATTTTATTTTGACAATAGCATTTTAGATATTTTTTCAAGTG
TAAAATCAGGAGCGACTTTACACCTTTTACCCAATCATCTTTTTGCCTTTCCAAATAAAATTTTAGAATGCCTTGAAAAA
GAAAAAGTAAGCACTATATTTTGGGTACCTTCGGTGCTGATTTATTTTGCAAATACTGAAGCTTTGAATAATTTTAGTTT
AAACGCCTTAAAAAAGATACTTTTTTGTGGTGAAATCATGCCCAATAAACAGCTCAATATTTGGCGTAAACACTTACCCA
ATGCCTTATTTGCCAACCTTTATGGCCCAACAGAAATCACTGATGTATGTTCTTTTTATATCATCAATCGCACTTTTAAA
GATGAAGAACTTTTACCGATAGGAAAAGCGTGCAAAAACACAGAACTTTTAGTTTTTGATGAAAATATGAATTTCATAAG
TCCTAAACAAACAAGTGTTAAAGGGGAGCTTTTCGTGCGTGGAACTTCTTTATCTTTAGGGTATTATAATGACAAAGAAA
AAACCAAACAAGCTTTTACTCAAAATCCTTTACATGATAATTATTTAGATCTTTTGTACAAAACAGGAGATATAGTTTCT
TATAATGAATTTGGCGAACTTTTATGCTATGGAAGAGTGGATAATCAGATAAAATATATGGGGCATAGGATAGAACTTGG
AGAAATAGAAAGCGTGATAAATTCTCATGTGAATATTAAAAACAGTGCTTGTATTTTTAAAGAAGATATTATTTGTTTTT
ATGAAAGTGAAGAAGAAATTAATTTTAAAGATTTTTTGAAAGATAAATTACCTTCTTATATGATCCCAAAAAATTTTATT
AAACTCGACAAATTTAAGCTTAATCAAAATGGTAAAATTGATAGAAAGGTTTTAAAAGAACTTGTTTAA

Upstream 100 bases:

>100_bases
CATCACAAGAATCGATTTCAAAGCTTTGGTTTATGAGTATATAAAGGCCTTTTTTATTAATTTATCGATATTATATTACA
AAAAATTAAAAAGGTAAATC

Downstream 100 bases:

>100_bases
AAAAGCTTTTTATAAAGGATTTAAGTTAAGTAATTACTATGACAATTTTGGTACAATTGAAGAAAAAATTTTAAAACAGG
AATTTATTTTACAAAAATAT

Product: amino acid adenylation domain-containing protein

Products: NA

Alternate protein names: BA1; ATP-dependent isoleucine adenylase; IleA; Isoleucine activase; ATP-dependent cysteine adenylase; CysA; Cysteine activase; ATP-dependent leucine adenylase; LeuA; Leucine activase; ATP-dependent glutamate adenylase; GluA; Glutamate activase; ATP-dependent isoleucine adenylase; IleA; Isoleucine activase; Glutamate racemase [H]

Number of amino acids: Translated: 502; Mature: 501

Protein sequence:

>502_residues
MTTHIYDFLEKSLIKFSEKTAFVEPFAKERKEITYKDFDLFSKKLASEILKTLKNNKPTQAPVLIILPKGIDCLISFFGV
ALSGNFYTLLDEKSPKERVEKVIEVLKPKLFITSKDLNFNLDLPTLYTEDFESFNIDESLIKHAKEKHIDTNLLYVFFTS
GSTGIPKGVSIAHKSVIDYTFWVCETFKFDENEILANQAPFYFDNSILDIFSSVKSGATLHLLPNHLFAFPNKILECLEK
EKVSTIFWVPSVLIYFANTEALNNFSLNALKKILFCGEIMPNKQLNIWRKHLPNALFANLYGPTEITDVCSFYIINRTFK
DEELLPIGKACKNTELLVFDENMNFISPKQTSVKGELFVRGTSLSLGYYNDKEKTKQAFTQNPLHDNYLDLLYKTGDIVS
YNEFGELLCYGRVDNQIKYMGHRIELGEIESVINSHVNIKNSACIFKEDIICFYESEEEINFKDFLKDKLPSYMIPKNFI
KLDKFKLNQNGKIDRKVLKELV

Sequences:

>Translated_502_residues
MTTHIYDFLEKSLIKFSEKTAFVEPFAKERKEITYKDFDLFSKKLASEILKTLKNNKPTQAPVLIILPKGIDCLISFFGV
ALSGNFYTLLDEKSPKERVEKVIEVLKPKLFITSKDLNFNLDLPTLYTEDFESFNIDESLIKHAKEKHIDTNLLYVFFTS
GSTGIPKGVSIAHKSVIDYTFWVCETFKFDENEILANQAPFYFDNSILDIFSSVKSGATLHLLPNHLFAFPNKILECLEK
EKVSTIFWVPSVLIYFANTEALNNFSLNALKKILFCGEIMPNKQLNIWRKHLPNALFANLYGPTEITDVCSFYIINRTFK
DEELLPIGKACKNTELLVFDENMNFISPKQTSVKGELFVRGTSLSLGYYNDKEKTKQAFTQNPLHDNYLDLLYKTGDIVS
YNEFGELLCYGRVDNQIKYMGHRIELGEIESVINSHVNIKNSACIFKEDIICFYESEEEINFKDFLKDKLPSYMIPKNFI
KLDKFKLNQNGKIDRKVLKELV
>Mature_501_residues
TTHIYDFLEKSLIKFSEKTAFVEPFAKERKEITYKDFDLFSKKLASEILKTLKNNKPTQAPVLIILPKGIDCLISFFGVA
LSGNFYTLLDEKSPKERVEKVIEVLKPKLFITSKDLNFNLDLPTLYTEDFESFNIDESLIKHAKEKHIDTNLLYVFFTSG
STGIPKGVSIAHKSVIDYTFWVCETFKFDENEILANQAPFYFDNSILDIFSSVKSGATLHLLPNHLFAFPNKILECLEKE
KVSTIFWVPSVLIYFANTEALNNFSLNALKKILFCGEIMPNKQLNIWRKHLPNALFANLYGPTEITDVCSFYIINRTFKD
EELLPIGKACKNTELLVFDENMNFISPKQTSVKGELFVRGTSLSLGYYNDKEKTKQAFTQNPLHDNYLDLLYKTGDIVSY
NEFGELLCYGRVDNQIKYMGHRIELGEIESVINSHVNIKNSACIFKEDIICFYESEEEINFKDFLKDKLPSYMIPKNFIK
LDKFKLNQNGKIDRKVLKELV

Specific function: Activates five amino acids, incorporates two D-amino acids, releases and cyclizes the mature bacitracin [H]

COG id: COG1020

COG function: function code Q; Non-ribosomal peptide synthetase modules and related proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 5 acyl carrier domains [H]

Homologues:

Organism=Homo sapiens, GI45580730, Length=369, Percent_Identity=27.3712737127371, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI156151445, Length=411, Percent_Identity=23.6009732360097, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI187761345, Length=536, Percent_Identity=21.455223880597, Blast_Score=96, Evalue=7e-20,
Organism=Homo sapiens, GI187761343, Length=536, Percent_Identity=21.455223880597, Blast_Score=96, Evalue=7e-20,
Organism=Homo sapiens, GI42544132, Length=336, Percent_Identity=24.4047619047619, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI115511026, Length=328, Percent_Identity=25, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI1786801, Length=463, Percent_Identity=26.9978401727862, Blast_Score=177, Evalue=1e-45,
Organism=Escherichia coli, GI145693145, Length=382, Percent_Identity=22.5130890052356, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1788107, Length=553, Percent_Identity=21.880650994575, Blast_Score=75, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17556356, Length=485, Percent_Identity=26.8041237113402, Blast_Score=115, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI17560308, Length=366, Percent_Identity=26.2295081967213, Blast_Score=108, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI71994694, Length=371, Percent_Identity=25.0673854447439, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI71994703, Length=371, Percent_Identity=25.0673854447439, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI71994690, Length=371, Percent_Identity=25.0673854447439, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17550940, Length=497, Percent_Identity=24.5472837022133, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17558820, Length=508, Percent_Identity=23.8188976377953, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17559526, Length=377, Percent_Identity=24.4031830238727, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI71985884, Length=330, Percent_Identity=22.7272727272727, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI32564422, Length=355, Percent_Identity=23.0985915492958, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI32564420, Length=355, Percent_Identity=23.0985915492958, Blast_Score=83, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17531443, Length=324, Percent_Identity=23.1481481481481, Blast_Score=79, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17557194, Length=371, Percent_Identity=25.0673854447439, Blast_Score=76, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17538037, Length=371, Percent_Identity=25.0673854447439, Blast_Score=72, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17560140, Length=304, Percent_Identity=20.7236842105263, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6319591, Length=596, Percent_Identity=24.8322147651007, Blast_Score=152, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6319699, Length=363, Percent_Identity=22.3140495867769, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24648676, Length=473, Percent_Identity=24.9471458773784, Blast_Score=145, Evalue=8e-35,
Organism=Drosophila melanogaster, GI24581924, Length=356, Percent_Identity=26.685393258427, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI18859661, Length=394, Percent_Identity=23.8578680203046, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI21355181, Length=412, Percent_Identity=24.5145631067961, Blast_Score=89, Evalue=7e-18,
Organism=Drosophila melanogaster, GI24582852, Length=378, Percent_Identity=24.6031746031746, Blast_Score=88, Evalue=2e-17,
Organism=Drosophila melanogaster, GI281365686, Length=389, Percent_Identity=23.6503856041131, Blast_Score=71, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010071
- InterPro:   IPR009081
- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR001242
- InterPro:   IPR013624
- InterPro:   IPR010060
- InterPro:   IPR006163
- InterPro:   IPR006162 [H]

Pfam domain/function: PF00501 AMP-binding; PF00668 Condensation; PF08415 NRPS; PF00550 PP-binding [H]

EC number: =5.1.1.3 [H]

Molecular weight: Translated: 57944; Mature: 57813

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTHIYDFLEKSLIKFSEKTAFVEPFAKERKEITYKDFDLFSKKLASEILKTLKNNKPTQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
APVLIILPKGIDCLISFFGVALSGNFYTLLDEKSPKERVEKVIEVLKPKLFITSKDLNFN
CCEEEEECCCHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHCCEEEEEECCCEEE
LDLPTLYTEDFESFNIDESLIKHAKEKHIDTNLLYVFFTSGSTGIPKGVSIAHKSVIDYT
CCCCCHHHCCCCCCCCCHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHH
FWVCETFKFDENEILANQAPFYFDNSILDIFSSVKSGATLHLLPNHLFAFPNKILECLEK
EEEEEEEECCCCCEECCCCCCEECCHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHH
EKVSTIFWVPSVLIYFANTEALNNFSLNALKKILFCGEIMPNKQLNIWRKHLPNALFANL
HCCCEEEEHHHHHHEECCCHHCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHC
YGPTEITDVCSFYIINRTFKDEELLPIGKACKNTELLVFDENMNFISPKQTSVKGELFVR
CCCCHHHHHHHHHHHCCCCCCCCCCCCHHCCCCCEEEEEECCCCCCCCCCCCCCEEEEEE
GTSLSLGYYNDKEKTKQAFTQNPLHDNYLDLLYKTGDIVSYNEFGELLCYGRVDNQIKYM
EEEEEECCCCCHHHHHHHHHCCCCCCHHHHHHHHCCCEEEECCCCCEEEECCCCCHHHHC
GHRIELGEIESVINSHVNIKNSACIFKEDIICFYESEEEINFKDFLKDKLPSYMIPKNFI
CCCCCHHHHHHHHHCCCCCCCCEEEEECCEEEEECCCCCCCHHHHHHHHCCCCCCCHHHH
KLDKFKLNQNGKIDRKVLKELV
EEEEEEECCCCCHHHHHHHHHC
>Mature Secondary Structure 
TTHIYDFLEKSLIKFSEKTAFVEPFAKERKEITYKDFDLFSKKLASEILKTLKNNKPTQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
APVLIILPKGIDCLISFFGVALSGNFYTLLDEKSPKERVEKVIEVLKPKLFITSKDLNFN
CCEEEEECCCHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHCCEEEEEECCCEEE
LDLPTLYTEDFESFNIDESLIKHAKEKHIDTNLLYVFFTSGSTGIPKGVSIAHKSVIDYT
CCCCCHHHCCCCCCCCCHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHH
FWVCETFKFDENEILANQAPFYFDNSILDIFSSVKSGATLHLLPNHLFAFPNKILECLEK
EEEEEEEECCCCCEECCCCCCEECCHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHH
EKVSTIFWVPSVLIYFANTEALNNFSLNALKKILFCGEIMPNKQLNIWRKHLPNALFANL
HCCCEEEEHHHHHHEECCCHHCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHC
YGPTEITDVCSFYIINRTFKDEELLPIGKACKNTELLVFDENMNFISPKQTSVKGELFVR
CCCCHHHHHHHHHHHCCCCCCCCCCCCHHCCCCCEEEEEECCCCCCCCCCCCCCEEEEEE
GTSLSLGYYNDKEKTKQAFTQNPLHDNYLDLLYKTGDIVSYNEFGELLCYGRVDNQIKYM
EEEEEECCCCCHHHHHHHHHCCCCCCHHHHHHHHCCCEEEECCCCCEEEECCCCCHHHHC
GHRIELGEIESVINSHVNIKNSACIFKEDIICFYESEEEINFKDFLKDKLPSYMIPKNFI
CCCCCHHHHHHHHHCCCCCCCCEEEEECCEEEEECCCCCCCHHHHHHHHCCCCCCCHHHH
KLDKFKLNQNGKIDRKVLKELV
EEEEEEECCCCCHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9427658 [H]