Click here to switch to the map view.

The map label for this gene is pbpA [H]

Identifier: 57238289

GI number: 57238289

Start: 551127

End: 553058

Strand: Direct

Name: pbpA [H]

Synonym: CJE0615

Alternate gene names: 57238289

Gene position: 551127-553058 (Clockwise)

Preceding gene: 57238290

Following gene: 57238286

Centisome position: 31.0

GC content: 33.02

Gene sequence:

>1932_bases
ATGAAAATATTAAAATATATTTTTTCATTTTTTACTCTTTTGTTTATAGCTGGATTTATTTATGTGGCTTATCTTTTCAC
AAGTGCTGATACTGAGGGTTATACTTTTAAAGAATACAAACCTCCTCTTACAACACAAATTTACGATAGAAATGGCAAGC
TTGTGGCTAATATTTTTGAACAACATCGTTTTTACGCTCCTTATGAAGAGCTTCCTCCACGCCTTATAGAAGCTTTGGTT
GCTATAGAAGATACAAGCTTTTTTGAACATAATGGAGTTAATATCGATGCCATTTTTAGAGCTGCTGTAAAAATCATAAA
AAGCGGTGGAAAAACCATGGAAGGAGCTTCCACTCTTACCCAGCAATTTATTAAAAACACGGAATTAACCCCTGAAAGAA
CTATTACGCGTAAAATTCGCGAAGCTTTGCTTGCTTATAAAATGGAAACCATTTTAACTAAAGAGCAAATTTTAGAAAGG
TATTTAAATTTTATTTTTTTTGGACATGGGTATTATGGAGTAAAAACCGCAGCTTTGGGGTATTTTCATAAAAATTTAAA
TGAATTAAGCCTTAAAGAGATTGCTATGTTAGTAGGTATGCCAAAAGCACCTAGCAGTTATGATCCTACAAAGCATTTAG
ATCTTTCCATTTCAAGAGCAAATAATGTTATTTCAAGAATGTATAATCTAGGTTGGATTTCAAAAGCAGATTATGATACA
GCCATTAAAGAAATTCCGCAAGTTTATGATGATACGCTTACTCAAAATGCTGCTCCTTATGTCGTAGATGAAGTGATTAA
ACAATTAAGCCCTAATATCAAAGACTTAAAAACAGGTGGATATAAAATCATTTTAAATATAGACTTAGATGTTCAAAATA
TGGCACAGAATGCTTTAAAATTTGGTTATGATGAGATAGTAAAACGCGATAAAGATGCAAATTTAAGCACTTTAAACGGA
GCTATGGTCGTGGTAAATCATCAAAGTGGTGATGTTTTAGCTTTGGTAGGTGGAGTTGATTATGAAAAAAGTAATTATAA
CCGCGCTACTCAAAGTATGCGTCAACCTGGAAGCTCTTTTAAACCTTTTGTGTATCAAGTGGCGATTAATTTAGGATATT
CGCCTATGAGTGAAATTGCTGATATTTCTAGAATTTTTGAAGGTGGAGCGGGTAATAATGAGGATTGGAAACCTAAAAAT
GAAGGGGGTAAATTTTTAGGTTTAATTACCTTAAAAGAAGCTTTAACAAGATCAAGAAATTTAGCTACCATTAATCTCGC
ACTTGATATGGGGCTTGATGTTCTTTACTCTAAACTAATGGAATTTGGATTTAAAGATATACCGCCTAATTTATCAATCG
TATTGGGAAGTTTTGGTATTTCTCCTTTGGAGTATTCGAAATTTTATACCATGTTTGGGAACTATGGTACGATTAAAGAT
CCTCAAATTATCAGACAAGTTCAGGATAAAACCGGTAAAACCATCATGGAATTTAACTCAAATGAGCGTAAAGTCAGCGA
TGAAGCGCAAAGTTTTCTGGTTTTAGATATGATGAGAAATGTTGTTGAAAAAGGCACAGGGCGTAATGCAAGAGTAAAAG
ATATAGAAATAGCAGGAAAAACAGGAACAACGAATAAAAGTGTTGATGCTTGGTTTTGTGGACTTACGCCTGAAATAGAA
GCAATCATTTGGTATGGAAATGATAACAACAAGCCTATGCGTTATACAGAAGGTGGGGCTAGAACAGCTGCTCCTGTGTT
TAGAGAATTTTTAACTCAATATATAGAAAAATTCCCTGATACAACTAGAAAATTTAGCATTCCTAATGGAGTTTATCGTG
GAAATTATAAAGGAGAAAGTGCTTATTATACCACCAAATCACCATTACCAAAAGCAAATATGAAATTTAATGAAAGCGAG
ATTATATTTTGA

Upstream 100 bases:

>100_bases
GTATTATGTGTGAGGGTTTTCATCAAAATTTCATCACCCAACAAGTAGGAAATCTAAGCACTGCTTTAGGGCTTGATATC
CAAACTTTAAAGGCTTATTT

Downstream 100 bases:

>100_bases
AAGTAGAAAGATCTACTTTCAAAATGATTAAGCTTTTTTGATGATTATTTCATCATCTTTTGCATCAATGATAATGCTAT
CATTTTCATGAAGTTCATCA

Product: penicillin-binding protein 1A

Products: NA

Alternate protein names: PBP-1F; Penicillin-binding protein F [H]

Number of amino acids: Translated: 643; Mature: 643

Protein sequence:

>643_residues
MKILKYIFSFFTLLFIAGFIYVAYLFTSADTEGYTFKEYKPPLTTQIYDRNGKLVANIFEQHRFYAPYEELPPRLIEALV
AIEDTSFFEHNGVNIDAIFRAAVKIIKSGGKTMEGASTLTQQFIKNTELTPERTITRKIREALLAYKMETILTKEQILER
YLNFIFFGHGYYGVKTAALGYFHKNLNELSLKEIAMLVGMPKAPSSYDPTKHLDLSISRANNVISRMYNLGWISKADYDT
AIKEIPQVYDDTLTQNAAPYVVDEVIKQLSPNIKDLKTGGYKIILNIDLDVQNMAQNALKFGYDEIVKRDKDANLSTLNG
AMVVVNHQSGDVLALVGGVDYEKSNYNRATQSMRQPGSSFKPFVYQVAINLGYSPMSEIADISRIFEGGAGNNEDWKPKN
EGGKFLGLITLKEALTRSRNLATINLALDMGLDVLYSKLMEFGFKDIPPNLSIVLGSFGISPLEYSKFYTMFGNYGTIKD
PQIIRQVQDKTGKTIMEFNSNERKVSDEAQSFLVLDMMRNVVEKGTGRNARVKDIEIAGKTGTTNKSVDAWFCGLTPEIE
AIIWYGNDNNKPMRYTEGGARTAAPVFREFLTQYIEKFPDTTRKFSIPNGVYRGNYKGESAYYTTKSPLPKANMKFNESE
IIF

Sequences:

>Translated_643_residues
MKILKYIFSFFTLLFIAGFIYVAYLFTSADTEGYTFKEYKPPLTTQIYDRNGKLVANIFEQHRFYAPYEELPPRLIEALV
AIEDTSFFEHNGVNIDAIFRAAVKIIKSGGKTMEGASTLTQQFIKNTELTPERTITRKIREALLAYKMETILTKEQILER
YLNFIFFGHGYYGVKTAALGYFHKNLNELSLKEIAMLVGMPKAPSSYDPTKHLDLSISRANNVISRMYNLGWISKADYDT
AIKEIPQVYDDTLTQNAAPYVVDEVIKQLSPNIKDLKTGGYKIILNIDLDVQNMAQNALKFGYDEIVKRDKDANLSTLNG
AMVVVNHQSGDVLALVGGVDYEKSNYNRATQSMRQPGSSFKPFVYQVAINLGYSPMSEIADISRIFEGGAGNNEDWKPKN
EGGKFLGLITLKEALTRSRNLATINLALDMGLDVLYSKLMEFGFKDIPPNLSIVLGSFGISPLEYSKFYTMFGNYGTIKD
PQIIRQVQDKTGKTIMEFNSNERKVSDEAQSFLVLDMMRNVVEKGTGRNARVKDIEIAGKTGTTNKSVDAWFCGLTPEIE
AIIWYGNDNNKPMRYTEGGARTAAPVFREFLTQYIEKFPDTTRKFSIPNGVYRGNYKGESAYYTTKSPLPKANMKFNESE
IIF
>Mature_643_residues
MKILKYIFSFFTLLFIAGFIYVAYLFTSADTEGYTFKEYKPPLTTQIYDRNGKLVANIFEQHRFYAPYEELPPRLIEALV
AIEDTSFFEHNGVNIDAIFRAAVKIIKSGGKTMEGASTLTQQFIKNTELTPERTITRKIREALLAYKMETILTKEQILER
YLNFIFFGHGYYGVKTAALGYFHKNLNELSLKEIAMLVGMPKAPSSYDPTKHLDLSISRANNVISRMYNLGWISKADYDT
AIKEIPQVYDDTLTQNAAPYVVDEVIKQLSPNIKDLKTGGYKIILNIDLDVQNMAQNALKFGYDEIVKRDKDANLSTLNG
AMVVVNHQSGDVLALVGGVDYEKSNYNRATQSMRQPGSSFKPFVYQVAINLGYSPMSEIADISRIFEGGAGNNEDWKPKN
EGGKFLGLITLKEALTRSRNLATINLALDMGLDVLYSKLMEFGFKDIPPNLSIVLGSFGISPLEYSKFYTMFGNYGTIKD
PQIIRQVQDKTGKTIMEFNSNERKVSDEAQSFLVLDMMRNVVEKGTGRNARVKDIEIAGKTGTTNKSVDAWFCGLTPEIE
AIIWYGNDNNKPMRYTEGGARTAAPVFREFLTQYIEKFPDTTRKFSIPNGVYRGNYKGESAYYTTKSPLPKANMKFNESE
IIF

Specific function: Cell wall formation. May be involved in outgrowth of the germinated spore or it could function in the synthesis of the germ cell wall [H]

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=589, Percent_Identity=32.76740237691, Blast_Score=267, Evalue=2e-72,
Organism=Escherichia coli, GI87082258, Length=306, Percent_Identity=38.2352941176471, Blast_Score=202, Evalue=4e-53,
Organism=Escherichia coli, GI1788867, Length=569, Percent_Identity=28.2952548330404, Blast_Score=176, Evalue=3e-45,
Organism=Escherichia coli, GI1789601, Length=170, Percent_Identity=33.5294117647059, Blast_Score=92, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.1.129; 3.4.-.- [C]

Molecular weight: Translated: 72629; Mature: 72629

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILKYIFSFFTLLFIAGFIYVAYLFTSADTEGYTFKEYKPPLTTQIYDRNGKLVANIFE
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCCCHHHHHH
QHRFYAPYEELPPRLIEALVAIEDTSFFEHNGVNIDAIFRAAVKIIKSGGKTMEGASTLT
HHCCCCCHHHCCHHHHHHHHHHCCCCCHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHH
QQFIKNTELTPERTITRKIREALLAYKMETILTKEQILERYLNFIFFGHGYYGVKTAALG
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHH
YFHKNLNELSLKEIAMLVGMPKAPSSYDPTKHLDLSISRANNVISRMYNLGWISKADYDT
HHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHCCCCCCCCHHH
AIKEIPQVYDDTLTQNAAPYVVDEVIKQLSPNIKDLKTGGYKIILNIDLDVQNMAQNALK
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHCCCCEEEEEEECCCHHHHHHHHHH
FGYDEIVKRDKDANLSTLNGAMVVVNHQSGDVLALVGGVDYEKSNYNRATQSMRQPGSSF
CCHHHHHHCCCCCCCEEECCEEEEEECCCCCEEEEECCCCCCCCCCHHHHHHHHCCCCCC
KPFVYQVAINLGYSPMSEIADISRIFEGGAGNNEDWKPKNEGGKFLGLITLKEALTRSRN
CCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHCCCC
LATINLALDMGLDVLYSKLMEFGFKDIPPNLSIVLGSFGISPLEYSKFYTMFGNYGTIKD
EEEEEEEHHHCHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCC
PQIIRQVQDKTGKTIMEFNSNERKVSDEAQSFLVLDMMRNVVEKGTGRNARVKDIEIAGK
HHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECC
TGTTNKSVDAWFCGLTPEIEAIIWYGNDNNKPMRYTEGGARTAAPVFREFLTQYIEKFPD
CCCCCCCCCEEEECCCCCEEEEEEEECCCCCCCEECCCCCCHHHHHHHHHHHHHHHHCCC
TTRKFSIPNGVYRGNYKGESAYYTTKSPLPKANMKFNESEIIF
CCEEECCCCCCEECCCCCCCCEEECCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKILKYIFSFFTLLFIAGFIYVAYLFTSADTEGYTFKEYKPPLTTQIYDRNGKLVANIFE
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCCCHHHHHH
QHRFYAPYEELPPRLIEALVAIEDTSFFEHNGVNIDAIFRAAVKIIKSGGKTMEGASTLT
HHCCCCCHHHCCHHHHHHHHHHCCCCCHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHH
QQFIKNTELTPERTITRKIREALLAYKMETILTKEQILERYLNFIFFGHGYYGVKTAALG
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHH
YFHKNLNELSLKEIAMLVGMPKAPSSYDPTKHLDLSISRANNVISRMYNLGWISKADYDT
HHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHCCCCCCCCHHH
AIKEIPQVYDDTLTQNAAPYVVDEVIKQLSPNIKDLKTGGYKIILNIDLDVQNMAQNALK
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHCCCCEEEEEEECCCHHHHHHHHHH
FGYDEIVKRDKDANLSTLNGAMVVVNHQSGDVLALVGGVDYEKSNYNRATQSMRQPGSSF
CCHHHHHHCCCCCCCEEECCEEEEEECCCCCEEEEECCCCCCCCCCHHHHHHHHCCCCCC
KPFVYQVAINLGYSPMSEIADISRIFEGGAGNNEDWKPKNEGGKFLGLITLKEALTRSRN
CCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHCCCC
LATINLALDMGLDVLYSKLMEFGFKDIPPNLSIVLGSFGISPLEYSKFYTMFGNYGTIKD
EEEEEEEHHHCHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCC
PQIIRQVQDKTGKTIMEFNSNERKVSDEAQSFLVLDMMRNVVEKGTGRNARVKDIEIAGK
HHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECC
TGTTNKSVDAWFCGLTPEIEAIIWYGNDNNKPMRYTEGGARTAAPVFREFLTQYIEKFPD
CCCCCCCCCEEEECCCCCEEEEEEEECCCCCCCEECCCCCCHHHHHHHHHHHHHHHHCCC
TTRKFSIPNGVYRGNYKGESAYYTTKSPLPKANMKFNESEIIF
CCEEECCCCCCEECCCCCCCCEEECCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377; 8335642; 1459957 [H]