Click here to switch to the map view.

The map label for this gene is penA [H]

Identifier: 57238275

GI number: 57238275

Start: 565800

End: 567539

Strand: Reverse

Name: penA [H]

Synonym: CJE0629

Alternate gene names: 57238275

Gene position: 567539-565800 (Counterclockwise)

Preceding gene: 57238274

Following gene: 57238287

Centisome position: 31.92

GC content: 30.8

Gene sequence:

>1740_bases
ATGGCACTTTTGTTTATGATTATTTTTATCAGCTCTACTTTTTTTCTTACATCAAAACGTCATATACCTAACACTGAAAA
AGATCAATACGCTCTTGCTCTAAGAGGATCGATTATAACCAAAGATAATTTTACTATCACAAGTTCTAAACAAATTTATC
GTGCTGAAATTGATCTTAGAAGCATTAACAAAGATAAATTTGATCTATTTTTAAAGCTTTTTCAAATTTATAGTGGGATA
AGTAACGATCAAGTAGCAGATATTAAAAAAAGAATGCAAAATCAAAAAAAACGCTCTTATAATTTTGTTCTTTTACAAAA
CTTAGACTCCAAGCAAGCAAGCTACCTTAAAGATCTTGCTAAAAAACTTTATATACAAGGTTTTTTCAAAGCTTTTACAA
ATAACTCAGGAAGAGTTGAAACAAGAGGATTAAATATCATCGAACATGAAGAAGATAGAATTTACATGTCAAAAGATTCT
TTTACTCCTATTATCGGTTATACTAAAATGATATTAGATCCAGAAAGTGGAATTTTAAAAAATATAGGAGTTAAAGGTTT
AGAAAAATACTACGATGCATGTCTAAGCCCTGTTCAAAATGAAAAAATTCAAGGACTTAAAGATATAGGTGGAAATATTA
TTTTAAATCTAAATTCACTACAACAAAAAAAGATCAATGGTTGTGATTTATATTTAAATCTTTCTTTAAAACTGCAAAAA
AGTATAGAGAAAGCTATAGATCAAAGAAATGAGGATTTAAAAGCCAATGAAATCATCGTTGGGGTAATGGAAAGTAAAAC
AGGAAGAATTTTAGCTCTAGCAAGCTCAAGGCGTTATGACCCACAAAATCGCGGAAAGGATCTATCAGTTCTTAATGCAA
GCGCTATAGAATACGGCTATGAAGCAGGTTCTGTTATCAAACCTTTTATCTTTACAACAGCCTTAAGACTTGGTAAAATA
AAAATGGATGAAGTGATTAATACTTATGGCGGTTCTTACAAGCTAGGACGTTTCACCATCAAAGACGATCACAAAATGGA
CAAAATGACTATGGAGGAAGTTATAAGATATTCTTCAAATATAGGCATGATACAAATTGCTAAAAGACTTAACAATATAG
AAATCGTATCAGGGCTTAAAATATTTAAATTTGGCGAAAAAAGTGGTATTGATCTACCCTATGAACAAAAAGGAGAAATT
CCAAATCCTAAGCGCTTAAGAGATATAGAAAAATCTGTTTTAAGTTATGGTTATGGACTTAAAACAACCTTTATACAACT
CCTTGCAGCTTATAATGTTTTCAACAATGATGGGTTTTATATCACACCACGTTTAGCTGAAAAATTCTATCAAAATGGAC
GCTTTACAAATCTTGATGATGATGTAAAAAAAGAAAAAATTCTTTCTAGCGAAGCCGCTAAAACGATGCAAAATGTTCTT
ATAAATGTTATAGAAAAAGGAACAGGAAAAAAGGCAATTACACAAGGTATTATTGCAGGAGGTAAAACAGGAACGGCAAG
AATAGCCGAAAGACAAGGTTACACCTCAAATCGCTACAATGCATCCTTTTTTGGTTTTGCAAATGATTTAAATCACGCCT
ATACCATAGGAGTTCTCGTAAGACATCCTACTAAACCTTATAGTTATTATGCTGCACAAAGTGCTTTGCCTATGTTTAAA
GATGTTGTTGATATTTTAATCAATGAAGAATTTCTAACGCCTATACAAGACAATAACTAA

Upstream 100 bases:

>100_bases
AGCGATCAGTGCTTATAAAGAAATCACAAGAACTCAAATTTAAACCCTAATGCAAGAATATAAAAAAAATAGGGTTTCAA
AAGTTGCCTTTGCCTACTGC

Downstream 100 bases:

>100_bases
ACTAGCACAAACAACTAGTTTGATATATCTTTAGAAAACATCACCTCAACAGCATCTATAAGTTCTTCAGCAATCTTAGC
AGCAAAAGTTGTATCATTAA

Product: penicillin-binding protein

Products: NA

Alternate protein names: PBP-2 [H]

Number of amino acids: Translated: 579; Mature: 578

Protein sequence:

>579_residues
MALLFMIIFISSTFFLTSKRHIPNTEKDQYALALRGSIITKDNFTITSSKQIYRAEIDLRSINKDKFDLFLKLFQIYSGI
SNDQVADIKKRMQNQKKRSYNFVLLQNLDSKQASYLKDLAKKLYIQGFFKAFTNNSGRVETRGLNIIEHEEDRIYMSKDS
FTPIIGYTKMILDPESGILKNIGVKGLEKYYDACLSPVQNEKIQGLKDIGGNIILNLNSLQQKKINGCDLYLNLSLKLQK
SIEKAIDQRNEDLKANEIIVGVMESKTGRILALASSRRYDPQNRGKDLSVLNASAIEYGYEAGSVIKPFIFTTALRLGKI
KMDEVINTYGGSYKLGRFTIKDDHKMDKMTMEEVIRYSSNIGMIQIAKRLNNIEIVSGLKIFKFGEKSGIDLPYEQKGEI
PNPKRLRDIEKSVLSYGYGLKTTFIQLLAAYNVFNNDGFYITPRLAEKFYQNGRFTNLDDDVKKEKILSSEAAKTMQNVL
INVIEKGTGKKAITQGIIAGGKTGTARIAERQGYTSNRYNASFFGFANDLNHAYTIGVLVRHPTKPYSYYAAQSALPMFK
DVVDILINEEFLTPIQDNN

Sequences:

>Translated_579_residues
MALLFMIIFISSTFFLTSKRHIPNTEKDQYALALRGSIITKDNFTITSSKQIYRAEIDLRSINKDKFDLFLKLFQIYSGI
SNDQVADIKKRMQNQKKRSYNFVLLQNLDSKQASYLKDLAKKLYIQGFFKAFTNNSGRVETRGLNIIEHEEDRIYMSKDS
FTPIIGYTKMILDPESGILKNIGVKGLEKYYDACLSPVQNEKIQGLKDIGGNIILNLNSLQQKKINGCDLYLNLSLKLQK
SIEKAIDQRNEDLKANEIIVGVMESKTGRILALASSRRYDPQNRGKDLSVLNASAIEYGYEAGSVIKPFIFTTALRLGKI
KMDEVINTYGGSYKLGRFTIKDDHKMDKMTMEEVIRYSSNIGMIQIAKRLNNIEIVSGLKIFKFGEKSGIDLPYEQKGEI
PNPKRLRDIEKSVLSYGYGLKTTFIQLLAAYNVFNNDGFYITPRLAEKFYQNGRFTNLDDDVKKEKILSSEAAKTMQNVL
INVIEKGTGKKAITQGIIAGGKTGTARIAERQGYTSNRYNASFFGFANDLNHAYTIGVLVRHPTKPYSYYAAQSALPMFK
DVVDILINEEFLTPIQDNN
>Mature_578_residues
ALLFMIIFISSTFFLTSKRHIPNTEKDQYALALRGSIITKDNFTITSSKQIYRAEIDLRSINKDKFDLFLKLFQIYSGIS
NDQVADIKKRMQNQKKRSYNFVLLQNLDSKQASYLKDLAKKLYIQGFFKAFTNNSGRVETRGLNIIEHEEDRIYMSKDSF
TPIIGYTKMILDPESGILKNIGVKGLEKYYDACLSPVQNEKIQGLKDIGGNIILNLNSLQQKKINGCDLYLNLSLKLQKS
IEKAIDQRNEDLKANEIIVGVMESKTGRILALASSRRYDPQNRGKDLSVLNASAIEYGYEAGSVIKPFIFTTALRLGKIK
MDEVINTYGGSYKLGRFTIKDDHKMDKMTMEEVIRYSSNIGMIQIAKRLNNIEIVSGLKIFKFGEKSGIDLPYEQKGEIP
NPKRLRDIEKSVLSYGYGLKTTFIQLLAAYNVFNNDGFYITPRLAEKFYQNGRFTNLDDDVKKEKILSSEAAKTMQNVLI
NVIEKGTGKKAITQGIIAGGKTGTARIAERQGYTSNRYNASFFGFANDLNHAYTIGVLVRHPTKPYSYYAAQSALPMFKD
VVDILINEEFLTPIQDNN

Specific function: Synthesis of cross-linked peptidoglycan from the lipid intermediates [H]

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786272, Length=419, Percent_Identity=26.2529832935561, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1786854, Length=470, Percent_Identity=24.2553191489362, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005311
- InterPro:   IPR001460 [H]

Pfam domain/function: PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: NA

Molecular weight: Translated: 65686; Mature: 65554

Theoretical pI: Translated: 9.88; Mature: 9.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALLFMIIFISSTFFLTSKRHIPNTEKDQYALALRGSIITKDNFTITSSKQIYRAEIDLR
CHHHHHHHHHHHHHEEEECCCCCCCCCCCEEEEEECCEEECCCEEEECCCCEEEEECCHH
SINKDKFDLFLKLFQIYSGISNDQVADIKKRMQNQKKRSYNFVLLQNLDSKQASYLKDLA
HCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHH
KKLYIQGFFKAFTNNSGRVETRGLNIIEHEEDRIYMSKDSFTPIIGYTKMILDPESGILK
HHHHHHHHHHHHCCCCCEEEECCCCEEEECCCEEEEECCCCCCHHCCEEEEECCCCCHHH
NIGVKGLEKYYDACLSPVQNEKIQGLKDIGGNIILNLNSLQQKKINGCDLYLNLSLKLQK
HCCHHHHHHHHHHHCCCCCCHHHCCHHHCCCEEEEEECHHHHHCCCCEEEEEEEEHHHHH
SIEKAIDQRNEDLKANEIIVGVMESKTGRILALASSRRYDPQNRGKDLSVLNASAIEYGY
HHHHHHHHCCCCCCCCEEEEEEEECCCCCEEEEECCCCCCCCCCCCCEEEEEHHHHHCCC
EAGSVIKPFIFTTALRLGKIKMDEVINTYGGSYKLGRFTIKDDHKMDKMTMEEVIRYSSN
CCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHCCC
IGMIQIAKRLNNIEIVSGLKIFKFGEKSGIDLPYEQKGEIPNPKRLRDIEKSVLSYGYGL
CCHHHHHHHCCCEEEECCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCH
KTTFIQLLAAYNVFNNDGFYITPRLAEKFYQNGRFTNLDDDVKKEKILSSEAAKTMQNVL
HHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
INVIEKGTGKKAITQGIIAGGKTGTARIAERQGYTSNRYNASFFGFANDLNHAYTIGVLV
HHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCEEEEECCCCCCEEEEEEEE
RHPTKPYSYYAAQSALPMFKDVVDILINEEFLTPIQDNN
ECCCCCCHHHHHHHCCHHHHHHHHHHHCHHHCCCCCCCC
>Mature Secondary Structure 
ALLFMIIFISSTFFLTSKRHIPNTEKDQYALALRGSIITKDNFTITSSKQIYRAEIDLR
HHHHHHHHHHHHHEEEECCCCCCCCCCCEEEEEECCEEECCCEEEECCCCEEEEECCHH
SINKDKFDLFLKLFQIYSGISNDQVADIKKRMQNQKKRSYNFVLLQNLDSKQASYLKDLA
HCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHH
KKLYIQGFFKAFTNNSGRVETRGLNIIEHEEDRIYMSKDSFTPIIGYTKMILDPESGILK
HHHHHHHHHHHHCCCCCEEEECCCCEEEECCCEEEEECCCCCCHHCCEEEEECCCCCHHH
NIGVKGLEKYYDACLSPVQNEKIQGLKDIGGNIILNLNSLQQKKINGCDLYLNLSLKLQK
HCCHHHHHHHHHHHCCCCCCHHHCCHHHCCCEEEEEECHHHHHCCCCEEEEEEEEHHHHH
SIEKAIDQRNEDLKANEIIVGVMESKTGRILALASSRRYDPQNRGKDLSVLNASAIEYGY
HHHHHHHHCCCCCCCCEEEEEEEECCCCCEEEEECCCCCCCCCCCCCEEEEEHHHHHCCC
EAGSVIKPFIFTTALRLGKIKMDEVINTYGGSYKLGRFTIKDDHKMDKMTMEEVIRYSSN
CCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHCCC
IGMIQIAKRLNNIEIVSGLKIFKFGEKSGIDLPYEQKGEIPNPKRLRDIEKSVLSYGYGL
CCHHHHHHHCCCEEEECCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCH
KTTFIQLLAAYNVFNNDGFYITPRLAEKFYQNGRFTNLDDDVKKEKILSSEAAKTMQNVL
HHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
INVIEKGTGKKAITQGIIAGGKTGTARIAERQGYTSNRYNASFFGFANDLNHAYTIGVLV
HHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCEEEEECCCCCCEEEEEEEE
RHPTKPYSYYAAQSALPMFKDVVDILINEEFLTPIQDNN
ECCCCCCHHHHHHHCCHHHHHHHHHHHCHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3126399 [H]