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The map label for this gene is ispB [H]

Identifier: 57238259

GI number: 57238259

Start: 581477

End: 582370

Strand: Direct

Name: ispB [H]

Synonym: CJE0645

Alternate gene names: 57238259

Gene position: 581477-582370 (Clockwise)

Preceding gene: 57238260

Following gene: 57238258

Centisome position: 32.71

GC content: 28.3

Gene sequence:

>894_bases
GTGCAAAAAATAGATGAATTAATAAAACAGTTTTTACAAGAGCTTGGCTATGAACCTATTTTAAATATGCTTTCAAATGT
AAAATCAGGAAAGAAATTACGCTCTAAATTATTATTAACTATCGCAGATGAGAGTGAGATTGCTTTTAAAATTTGTGCGG
CTATAGAATTGATACATCTTGCTAGTTTATTACATGATGATATTATCGATGAGAGTGAATTAAGGCGTGGAGCAAGATCT
GTTAATGCTGAATTTGGTACAAAAAATGCTCTTATGTTGGGCGATATTTTGTATTCAAAAGCCTTTTATGAATTAAGCAA
AATGGATGCTAGATTTGCAAGTATTATTTCAGATGCTGTTGTAAAGCTTGCTATAGGCGAACTTATGGATGTTGATTTGG
GAGAGAAATTTAATATCAATAAAGAAGCTTATTTGAAAATGATCTATAATAAAACAGCTGTTTTGATTGAAGCAAGTGCT
AGATGCGGAGCTATTTTGGCTGGATTATATGAAAAAGATTTTGCAGAATATGGTAAAAATTTAGGACTTGCTTTTCAGAT
GATTGATGATATTTTAGATATTAAGAGTGATGAAAAAATTCTTGGAAAACCTGCTATGAATGATTTTAAAGAGGGTAAAA
CTACCTTGCCTTATATTTATCTTTATGAAAATTTACAAGAACAAGATAGGATATATTTGCAGACTTTATTTAAAAAAGAT
TTAAATGAAAATGAAAAAGAATGGCTAAAAACTAAATTTGAAGAACAAAAAGCTTTAGAAAAAGCTATTTTAGAAGCTAA
AACTTATGCAAAAAAGGCTAGAAAAGCAATTGAAAAATATGATAATAACAAGCTTAATGATATTATTAAAGCTATGATAG
ATAGGGAATTTTAA

Upstream 100 bases:

>100_bases
CAAAATAATCAGGTTGTTCCTATGATTAATTTTAACGAACCTCAGGTAAAACCATTAACAAGCAAAGAAAATAATAATAG
CAATTAACAAGGAAAAGATA

Downstream 100 bases:

>100_bases
TGTATTATTGTATATCTTTTACTCATAAAAATACAGATATTGCCTTAAGGGAAAAGTTAAGCTTTTCCAATGAAGCTAAA
AAAAGTGAGTTTTTAAAAAT

Product: polyprenyl synthetase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MQKIDELIKQFLQELGYEPILNMLSNVKSGKKLRSKLLLTIADESEIAFKICAAIELIHLASLLHDDIIDESELRRGARS
VNAEFGTKNALMLGDILYSKAFYELSKMDARFASIISDAVVKLAIGELMDVDLGEKFNINKEAYLKMIYNKTAVLIEASA
RCGAILAGLYEKDFAEYGKNLGLAFQMIDDILDIKSDEKILGKPAMNDFKEGKTTLPYIYLYENLQEQDRIYLQTLFKKD
LNENEKEWLKTKFEEQKALEKAILEAKTYAKKARKAIEKYDNNKLNDIIKAMIDREF

Sequences:

>Translated_297_residues
MQKIDELIKQFLQELGYEPILNMLSNVKSGKKLRSKLLLTIADESEIAFKICAAIELIHLASLLHDDIIDESELRRGARS
VNAEFGTKNALMLGDILYSKAFYELSKMDARFASIISDAVVKLAIGELMDVDLGEKFNINKEAYLKMIYNKTAVLIEASA
RCGAILAGLYEKDFAEYGKNLGLAFQMIDDILDIKSDEKILGKPAMNDFKEGKTTLPYIYLYENLQEQDRIYLQTLFKKD
LNENEKEWLKTKFEEQKALEKAILEAKTYAKKARKAIEKYDNNKLNDIIKAMIDREF
>Mature_297_residues
MQKIDELIKQFLQELGYEPILNMLSNVKSGKKLRSKLLLTIADESEIAFKICAAIELIHLASLLHDDIIDESELRRGARS
VNAEFGTKNALMLGDILYSKAFYELSKMDARFASIISDAVVKLAIGELMDVDLGEKFNINKEAYLKMIYNKTAVLIEASA
RCGAILAGLYEKDFAEYGKNLGLAFQMIDDILDIKSDEKILGKPAMNDFKEGKTTLPYIYLYENLQEQDRIYLQTLFKKD
LNENEKEWLKTKFEEQKALEKAILEAKTYAKKARKAIEKYDNNKLNDIIKAMIDREF

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=186, Percent_Identity=36.0215053763441, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI4758430, Length=229, Percent_Identity=27.0742358078603, Blast_Score=66, Evalue=5e-11,
Organism=Homo sapiens, GI83700220, Length=229, Percent_Identity=27.0742358078603, Blast_Score=66, Evalue=5e-11,
Organism=Escherichia coli, GI1789578, Length=275, Percent_Identity=34.1818181818182, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI1786623, Length=291, Percent_Identity=28.5223367697594, Blast_Score=91, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI17505681, Length=232, Percent_Identity=35.7758620689655, Blast_Score=123, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6319475, Length=217, Percent_Identity=33.6405529953917, Blast_Score=114, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6325188, Length=210, Percent_Identity=25.7142857142857, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24651612, Length=181, Percent_Identity=39.2265193370166, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI281365769, Length=274, Percent_Identity=25.9124087591241, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24660002, Length=274, Percent_Identity=25.9124087591241, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 33996; Mature: 33996

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKIDELIKQFLQELGYEPILNMLSNVKSGKKLRSKLLLTIADESEIAFKICAAIELIHL
CCHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHEECCCHHHHHHHHHHHHHHHH
ASLLHDDIIDESELRRGARSVNAEFGTKNALMLGDILYSKAFYELSKMDARFASIISDAV
HHHHHHHCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VKLAIGELMDVDLGEKFNINKEAYLKMIYNKTAVLIEASARCGAILAGLYEKDFAEYGKN
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHCCC
LGLAFQMIDDILDIKSDEKILGKPAMNDFKEGKTTLPYIYLYENLQEQDRIYLQTLFKKD
CCHHHHHHHHHHCCCCCCHHHCCCCHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHHHH
LNENEKEWLKTKFEEQKALEKAILEAKTYAKKARKAIEKYDNNKLNDIIKAMIDREF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MQKIDELIKQFLQELGYEPILNMLSNVKSGKKLRSKLLLTIADESEIAFKICAAIELIHL
CCHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHEECCCHHHHHHHHHHHHHHHH
ASLLHDDIIDESELRRGARSVNAEFGTKNALMLGDILYSKAFYELSKMDARFASIISDAV
HHHHHHHCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VKLAIGELMDVDLGEKFNINKEAYLKMIYNKTAVLIEASARCGAILAGLYEKDFAEYGKN
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHCCC
LGLAFQMIDDILDIKSDEKILGKPAMNDFKEGKTTLPYIYLYENLQEQDRIYLQTLFKKD
CCHHHHHHHHHHCCCCCCHHHCCCCHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHHHH
LNENEKEWLKTKFEEQKALEKAILEAKTYAKKARKAIEKYDNNKLNDIIKAMIDREF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8312607; 9278503; 2670911; 8037730 [H]