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The map label for this gene is dnaB [H]

Identifier: 57238239

GI number: 57238239

Start: 603536

End: 604912

Strand: Direct

Name: dnaB [H]

Synonym: CJE0667

Alternate gene names: 57238239

Gene position: 603536-604912 (Clockwise)

Preceding gene: 57238241

Following gene: 57238238

Centisome position: 33.95

GC content: 33.26

Gene sequence:

>1377_bases
GTGCAGCAAGAGTATTATGACTTGGATTTAGAACGAGCTATTTTAAGCAGTTGTATCATGAGTGAAGAAGCTTATGCTAG
TATAGCAGGTGATATTAGCCCAAAAGATTTTAGTCTTAAAGCTCACCAAGATATTTTTAAAGCGGTTATAGCTTGTAGTA
ATAACAAAGAGCCCATTTCTGTAAGTTTTTTAAGAAAACATAAAAAAATAGATGAGCAAATTTTAGCAGAAATTCTTGCC
ACTCCTTCTATGATAGATTTGCCTGCTTATGTAAATGAACTTCACGAAAAGTCCGTAAAAAGACAACTCTTGAGTTTTGC
ACATCTTTTGCCAACTCGTATCAATGAAGATCGTGCTGTAAGTGAAATCGCAGATGAAATTGGTAAAGAAATTTTTAGCA
TTACAAATCGTGTGAATTCTAGAGATATTAAAGATGTTGATATGGTAATATCAGAACTTTTTGAAGAATTTAAAAAGCAA
AAAACTTTAGAAAATAAAGGTATTATAGGGCTTGATACAGGTTTTGAAGGTCTAAATAAAATGACAAAAGGCTTTAAAGG
GGGTGAACTTATAATAATCGCCGCGCGTCCAGGTATGGGAAAGACAACTCTTTGTTTAAATTTCATTGATAAGGTTTTAA
GACAAAAAAAAGGCGTGGCTTTATTTTCTCTTGAAATGCCTGCAACTCAAATCATGCAAAGAATGCTATCTTCTAAAACT
TCTATTCCTTTGCAAAAAATTTTAACTGCAGATTTAAACGATGATGAATGGGAGCGTTTAGGAGATGCTTGCAATGATTA
TAGTCAAAAGAAATTATATATTTACGATAGTGGTTATGCAACTATTGCTGATGTAAGGGCTATTTTAAGGCGTTTAAAAT
CTCAAGATGAAAGTATAGGACTTTGTGTGATTGACTATATAGGGCTTATGATGAGTAATTCAAATTTTAATGATAGACAT
TTGCAAGTTAGTGAAATTTCAAGAGGACTTAAGCTTTTGGCTAGGGAGCTTGATATGCCTATTATCGCACTTTCACAACT
TAATCGTGGCTTAGAGCAAAGGGCAAATAAGCGTCCTTTGATGAGTGATTTGCGAGAAAGTGGAGCTATAGAGCAAGATG
CGGATGCTATTTTGTTTGTATATCGCGATGAGGTTTATAGAGAACAAGAAGAAAAAGAAAGAGAGAATAAAGCTAAAGCT
GAAGGCAAAGCTTATCAAAGGCTTTTTATACCAAATCCTATGCAAGAAAATGCTGAAATTATTGTTGGAAAAAATAGAAA
TGGGCCTGTTGGAACTATAGAAGTTGTATTTTTAAAAGAAAAATCTTGTTTTGTAGATAAACCTATAGGCTATGAAACTA
CTGAATTTACAGGCTAG

Upstream 100 bases:

>100_bases
ACTAATATTACTTTAAATATTTATAAAAAAATTAAGTTTTATAATTTAAGTTTTTTTAGATAGAATTTTTAGAATTAATT
TATACTTATAAGGAAATTTA

Downstream 100 bases:

>100_bases
CTTTAATAAAATAAGTTTTTAATATTTTAAAGTTATTTTTTACGACACAACCTGTCGCAAGGGTGTTTTATACTTTCTTT
GTTATTTTAAAACAAGGAGA

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 458; Mature: 458

Protein sequence:

>458_residues
MQQEYYDLDLERAILSSCIMSEEAYASIAGDISPKDFSLKAHQDIFKAVIACSNNKEPISVSFLRKHKKIDEQILAEILA
TPSMIDLPAYVNELHEKSVKRQLLSFAHLLPTRINEDRAVSEIADEIGKEIFSITNRVNSRDIKDVDMVISELFEEFKKQ
KTLENKGIIGLDTGFEGLNKMTKGFKGGELIIIAARPGMGKTTLCLNFIDKVLRQKKGVALFSLEMPATQIMQRMLSSKT
SIPLQKILTADLNDDEWERLGDACNDYSQKKLYIYDSGYATIADVRAILRRLKSQDESIGLCVIDYIGLMMSNSNFNDRH
LQVSEISRGLKLLARELDMPIIALSQLNRGLEQRANKRPLMSDLRESGAIEQDADAILFVYRDEVYREQEEKERENKAKA
EGKAYQRLFIPNPMQENAEIIVGKNRNGPVGTIEVVFLKEKSCFVDKPIGYETTEFTG

Sequences:

>Translated_458_residues
MQQEYYDLDLERAILSSCIMSEEAYASIAGDISPKDFSLKAHQDIFKAVIACSNNKEPISVSFLRKHKKIDEQILAEILA
TPSMIDLPAYVNELHEKSVKRQLLSFAHLLPTRINEDRAVSEIADEIGKEIFSITNRVNSRDIKDVDMVISELFEEFKKQ
KTLENKGIIGLDTGFEGLNKMTKGFKGGELIIIAARPGMGKTTLCLNFIDKVLRQKKGVALFSLEMPATQIMQRMLSSKT
SIPLQKILTADLNDDEWERLGDACNDYSQKKLYIYDSGYATIADVRAILRRLKSQDESIGLCVIDYIGLMMSNSNFNDRH
LQVSEISRGLKLLARELDMPIIALSQLNRGLEQRANKRPLMSDLRESGAIEQDADAILFVYRDEVYREQEEKERENKAKA
EGKAYQRLFIPNPMQENAEIIVGKNRNGPVGTIEVVFLKEKSCFVDKPIGYETTEFTG
>Mature_458_residues
MQQEYYDLDLERAILSSCIMSEEAYASIAGDISPKDFSLKAHQDIFKAVIACSNNKEPISVSFLRKHKKIDEQILAEILA
TPSMIDLPAYVNELHEKSVKRQLLSFAHLLPTRINEDRAVSEIADEIGKEIFSITNRVNSRDIKDVDMVISELFEEFKKQ
KTLENKGIIGLDTGFEGLNKMTKGFKGGELIIIAARPGMGKTTLCLNFIDKVLRQKKGVALFSLEMPATQIMQRMLSSKT
SIPLQKILTADLNDDEWERLGDACNDYSQKKLYIYDSGYATIADVRAILRRLKSQDESIGLCVIDYIGLMMSNSNFNDRH
LQVSEISRGLKLLARELDMPIIALSQLNRGLEQRANKRPLMSDLRESGAIEQDADAILFVYRDEVYREQEEKERENKAKA
EGKAYQRLFIPNPMQENAEIIVGKNRNGPVGTIEVVFLKEKSCFVDKPIGYETTEFTG

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=458, Percent_Identity=35.8078602620087, Blast_Score=270, Evalue=1e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 51868; Mature: 51868

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQEYYDLDLERAILSSCIMSEEAYASIAGDISPKDFSLKAHQDIFKAVIACSNNKEPIS
CCCCHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEE
VSFLRKHKKIDEQILAEILATPSMIDLPAYVNELHEKSVKRQLLSFAHLLPTRINEDRAV
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
SEIADEIGKEIFSITNRVNSRDIKDVDMVISELFEEFKKQKTLENKGIIGLDTGFEGLNK
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHH
MTKGFKGGELIIIAARPGMGKTTLCLNFIDKVLRQKKGVALFSLEMPATQIMQRMLSSKT
HHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHCCC
SIPLQKILTADLNDDEWERLGDACNDYSQKKLYIYDSGYATIADVRAILRRLKSQDESIG
CCCHHHHHHCCCCCHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCC
LCVIDYIGLMMSNSNFNDRHLQVSEISRGLKLLARELDMPIIALSQLNRGLEQRANKRPL
CHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHH
MSDLRESGAIEQDADAILFVYRDEVYREQEEKERENKAKAEGKAYQRLFIPNPMQENAEI
HHHHHHCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCHHHHEEECCCCCCCCCEE
IVGKNRNGPVGTIEVVFLKEKSCFVDKPIGYETTEFTG
EEECCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MQQEYYDLDLERAILSSCIMSEEAYASIAGDISPKDFSLKAHQDIFKAVIACSNNKEPIS
CCCCHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEE
VSFLRKHKKIDEQILAEILATPSMIDLPAYVNELHEKSVKRQLLSFAHLLPTRINEDRAV
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
SEIADEIGKEIFSITNRVNSRDIKDVDMVISELFEEFKKQKTLENKGIIGLDTGFEGLNK
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHH
MTKGFKGGELIIIAARPGMGKTTLCLNFIDKVLRQKKGVALFSLEMPATQIMQRMLSSKT
HHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHCCC
SIPLQKILTADLNDDEWERLGDACNDYSQKKLYIYDSGYATIADVRAILRRLKSQDESIG
CCCHHHHHHCCCCCHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCC
LCVIDYIGLMMSNSNFNDRHLQVSEISRGLKLLARELDMPIIALSQLNRGLEQRANKRPL
CHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHH
MSDLRESGAIEQDADAILFVYRDEVYREQEEKERENKAKAEGKAYQRLFIPNPMQENAEI
HHHHHHCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCHHHHEEECCCCCCCCCEE
IVGKNRNGPVGTIEVVFLKEKSCFVDKPIGYETTEFTG
EEECCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9252185 [H]