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The map label for this gene is lpxD

Identifier: 57238228

GI number: 57238228

Start: 613366

End: 614331

Strand: Direct

Name: lpxD

Synonym: CJE0679

Alternate gene names: 57238228

Gene position: 613366-614331 (Clockwise)

Preceding gene: 57238229

Following gene: 57238223

Centisome position: 34.5

GC content: 33.13

Gene sequence:

>966_bases
ATGAAATTAAGTGAAATAGCAGAATTTTTAAGCTTGGAATATAAAGGCGAGGATATAGAAATCAGTGCTTTAAATTCTTT
ATTGAAAGCAAATTTTACAGAGCTTACTTATTGTGATGGTGAGAAAAATACCAAAGATATACCTCACACAGGAGCTGCTG
CTATTTTAGTATCTAAAGAATATGAAAATTTAGTACCTAAAGATACTAAAGCTTTGATTACTCAAAGTCCACATTTGAGT
TTTGCTTTTTTAAGCAAGCTTTTTGCTAAACCTTTGATAAGCACAGCTAAAGAAAAAGTACAAAATATCGCAAAAAGCGC
AAGAATCATGCCTAATGTTTATATAGGTGATAATGTAAATATCGGTGAAAATGTAATCATTATGGCGGGAGCTTATATAG
GCGATAATGTCAGCATTGGTGATGAGAGTATTATCCATCCTAATGTAGTGATTTATAATGATACTAAAATCGGTAAAAAA
TGTCATTTGCTTGCCAATTGTGTAATAGGAAGCGATGGCTTTGGTTATGCGCACAATAAAAACGGCGAACATTATAAAAT
TTATCATAATGGCAATGTGGTTTTAGAAGATTTTGTAGAAGTGGGGGCTTGTACAACTATAGATAGAGCAGTTTTTGATA
GTACTATTATCAAAGCAGGTACTAAGGTAGACAATCTAGTTCAAATCGGACATAATTGCAATATAGGTCAAAATTGTATC
ATTGTGGCACAAACTGGAATTTCAGGTTCAAGTGAGTTAGGACGCAATGTTATTATGGGTGGTCAAAGTGCAACGAGTGG
ACATTTAAAAATAGGCGATTTTAGTACTATAGCTGCAAGAGGCGGTGTGAGTAAAAATTTAGAAGGCGGGCGTGTTTATG
GAGGTTTTCCTATCATGCTTCAAAAAGATTGGCTAAAACTTCAAGCTAAAATAGCAATAAATTTTAAAGAAAAATCACAA
GATTAA

Upstream 100 bases:

>100_bases
TTGCTGATGATGCAAATCGCATTGATAATTTTTTAAAAACAATTAAAAAATACAATCCTAGTGATATAGTTCGCAGTGGA
TCTGTTTTGATGGAGATTAA

Downstream 100 bases:

>100_bases
CATCGCATCGCCATAAGAGTAAAAGCGATATTTTTCTTTTATGGCGAGTTCATAAAGTTTTAAACACTGTTCTCTACCGA
TAAAAGCTGAAACTAGCATG

Product: UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase

Products: UDP-2,3-Bis(3-Hydroxytetradecanoyl)Glucosamine; [Acyl-Carrier Protein]. [C]

Alternate protein names: NA

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MKLSEIAEFLSLEYKGEDIEISALNSLLKANFTELTYCDGEKNTKDIPHTGAAAILVSKEYENLVPKDTKALITQSPHLS
FAFLSKLFAKPLISTAKEKVQNIAKSARIMPNVYIGDNVNIGENVIIMAGAYIGDNVSIGDESIIHPNVVIYNDTKIGKK
CHLLANCVIGSDGFGYAHNKNGEHYKIYHNGNVVLEDFVEVGACTTIDRAVFDSTIIKAGTKVDNLVQIGHNCNIGQNCI
IVAQTGISGSSELGRNVIMGGQSATSGHLKIGDFSTIAARGGVSKNLEGGRVYGGFPIMLQKDWLKLQAKIAINFKEKSQ
D

Sequences:

>Translated_321_residues
MKLSEIAEFLSLEYKGEDIEISALNSLLKANFTELTYCDGEKNTKDIPHTGAAAILVSKEYENLVPKDTKALITQSPHLS
FAFLSKLFAKPLISTAKEKVQNIAKSARIMPNVYIGDNVNIGENVIIMAGAYIGDNVSIGDESIIHPNVVIYNDTKIGKK
CHLLANCVIGSDGFGYAHNKNGEHYKIYHNGNVVLEDFVEVGACTTIDRAVFDSTIIKAGTKVDNLVQIGHNCNIGQNCI
IVAQTGISGSSELGRNVIMGGQSATSGHLKIGDFSTIAARGGVSKNLEGGRVYGGFPIMLQKDWLKLQAKIAINFKEKSQ
D
>Mature_321_residues
MKLSEIAEFLSLEYKGEDIEISALNSLLKANFTELTYCDGEKNTKDIPHTGAAAILVSKEYENLVPKDTKALITQSPHLS
FAFLSKLFAKPLISTAKEKVQNIAKSARIMPNVYIGDNVNIGENVIIMAGAYIGDNVSIGDESIIHPNVVIYNDTKIGKK
CHLLANCVIGSDGFGYAHNKNGEHYKIYHNGNVVLEDFVEVGACTTIDRAVFDSTIIKAGTKVDNLVQIGHNCNIGQNCI
IVAQTGISGSSELGRNVIMGGQSATSGHLKIGDFSTIAARGGVSKNLEGGRVYGGFPIMLQKDWLKLQAKIAINFKEKSQ
D

Specific function: Lipid A biosynthesis; third step. [C]

COG id: COG1044

COG function: function code M; UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family. LpxD subfamily

Homologues:

Organism=Escherichia coli, GI1786376, Length=334, Percent_Identity=33.8323353293413, Blast_Score=180, Evalue=1e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LPXD_CAMJE (Q9PHU0)

Other databases:

- EMBL:   AL111168
- PIR:   G81404
- RefSeq:   YP_002344006.1
- ProteinModelPortal:   Q9PHU0
- SMR:   Q9PHU0
- IntAct:   Q9PHU0
- GeneID:   904901
- GenomeReviews:   AL111168_GR
- KEGG:   cje:Cj0576
- HOGENOM:   HBG469615
- OMA:   CFVGKNT
- PhylomeDB:   Q9PHU0
- ProtClustDB:   PRK00892
- BioCyc:   CJEJ192222:CJ0576-MONOMER
- HAMAP:   MF_00523
- InterPro:   IPR001451
- InterPro:   IPR018357
- InterPro:   IPR011004
- InterPro:   IPR007691
- InterPro:   IPR020573
- TIGRFAMs:   TIGR01853

Pfam domain/function: PF00132 Hexapep; PF04613 LpxD; SSF51161 Trimer_LpxA_like

EC number: 2.3.1.- [C]

Molecular weight: Translated: 34679; Mature: 34679

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLSEIAEFLSLEYKGEDIEISALNSLLKANFTELTYCDGEKNTKDIPHTGAAAILVSKE
CCHHHHHHHHEEEECCCCEEHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCEEEEEECC
YENLVPKDTKALITQSPHLSFAFLSKLFAKPLISTAKEKVQNIAKSARIMPNVYIGDNVN
HHHCCCCCCHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCC
IGENVIIMAGAYIGDNVSIGDESIIHPNVVIYNDTKIGKKCHLLANCVIGSDGFGYAHNK
CCCCEEEEEEEEECCCCCCCCCCEECCCEEEECCCCCCCCEEEEEEEEECCCCCCCCCCC
NGEHYKIYHNGNVVLEDFVEVGACTTIDRAVFDSTIIKAGTKVDNLVQIGHNCNIGQNCI
CCCEEEEEECCCEEEEHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHEECCCCCCCCCEE
IVAQTGISGSSELGRNVIMGGQSATSGHLKIGDFSTIAARGGVSKNLEGGRVYGGFPIML
EEEECCCCCCHHHCCEEEECCCCCCCCCEEECCCCEEHHCCCCCCCCCCCEEECCEEEEE
QKDWLKLQAKIAINFKEKSQD
ECCCEEEEEEEEEEECCCCCC
>Mature Secondary Structure
MKLSEIAEFLSLEYKGEDIEISALNSLLKANFTELTYCDGEKNTKDIPHTGAAAILVSKE
CCHHHHHHHHEEEECCCCEEHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCEEEEEECC
YENLVPKDTKALITQSPHLSFAFLSKLFAKPLISTAKEKVQNIAKSARIMPNVYIGDNVN
HHHCCCCCCHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCC
IGENVIIMAGAYIGDNVSIGDESIIHPNVVIYNDTKIGKKCHLLANCVIGSDGFGYAHNK
CCCCEEEEEEEEECCCCCCCCCCEECCCEEEECCCCCCCCEEEEEEEEECCCCCCCCCCC
NGEHYKIYHNGNVVLEDFVEVGACTTIDRAVFDSTIIKAGTKVDNLVQIGHNCNIGQNCI
CCCEEEEEECCCEEEEHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHEECCCCCCCCCEE
IVAQTGISGSSELGRNVIMGGQSATSGHLKIGDFSTIAARGGVSKNLEGGRVYGGFPIML
EEEECCCCCCHHHCCEEEECCCCCCCCCEEECCCCEEHHCCCCCCCCCCCEEECCEEEEE
QKDWLKLQAKIAINFKEKSQD
ECCCEEEEEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: UDP-3-O-(3-Hydroxytetradecanoyl)Glucosamine; (R)-3-Hydroxytetradecanoyl-[Acyl-Carrier Protein] [C]

Specific reaction: UDP-3-O-(3-Hydroxytetradecanoyl)Glucosamine + (R)-3-Hydroxytetradecanoyl-[Acyl-Carrier Protein] = UDP-2,3-Bis(3-Hydroxytetradecanoyl)Glucosamine + [Acyl-Carrier Protein]. [C]

General reaction: Acyltransferases; Transferring groups other than amino-acyl groups [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10688204