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The map label for this gene is queA [H]

Identifier: 57238227

GI number: 57238227

Start: 614320

End: 615348

Strand: Reverse

Name: queA [H]

Synonym: CJE0680

Alternate gene names: 57238227

Gene position: 615348-614320 (Counterclockwise)

Preceding gene: 57238226

Following gene: 57238240

Centisome position: 34.61

GC content: 31.78

Gene sequence:

>1029_bases
ATGAATAAAGACTTACTTCTTTCAAGCTATGATTACACTCTAGCTAATGAACTCATAGCCAACTATCCTACAAATCCAAA
AGAAGATGCTAGGCTTTTGGTTTTTGATCGCAAAAACAAAGAAATTTTTCATACTACTTTTAAAAATTTACAAGACTTTT
TACCCAACTGCGCGATTTTTTTCAATGATACCAAAGTTATTAAAGCAAGAATTTATGGCAATAAAGCAAGCGGAGGAAAA
ATAGAGCTTTTCTTGCATCAACCTTTTTTAAATTCTCACGATCCGCTTTTTCTAGCTCAAATTAAAGGGCGTGTTAAAAA
AGATGAAATCTTATATTTTAAAGATGATTTAAAAGTACGCGTTGTAGAACTTTTAAATGATGGTCTTCGAAAAGTTCAAT
TTTTTCAAAATGATAAAACCTTAGACACCTCAAATCTTTACAACCTGCTTGACAAGATAGGACATATACCCTTGCCTCCT
TATATTAAAAGGGAGGATGAAAAAAGTGATTTGAAAGATTATCAAAGTATCTTTGCAAAAAATTTAGGTGCAGTGGCTGC
TCCGACTGCAAGTTTACATTTTAGTGAGACTATGCTTGAAAATTTAAGAAAAAAACACGAAATTTATCATTTAACCTTAC
ATGTAGGAGCTGGAACCTTTAAAAGCGTGGAATGCGAAAATATACAAGAGCATAAAATGCATAGCGAATTTTTTAATATT
CCGCAGCAAGCATGTGAAATTATAGATTCTAAACAAGCTATTTTAGGCGTAGGAACAACGGTAACAAGAACCATAGAATA
TTATGCAAGAACTAAAACAAAAAATGGTTTTTGTGATCTTTTTTTACACCCGCAAAATCCACCTATAAGACAAAATCATC
TTTTAACCAATTTTCATTTACCAAAATCTACTCTTATCATGCTAGTTTCAGCTTTTATCGGTAGAGAACAGTGTTTAAAA
CTTTATGAACTCGCCATAAAAGAAAAATATCGCTTTTACTCTTATGGCGATGCGATGTTAATCTTGTGA

Upstream 100 bases:

>100_bases
TTATCTCAATTTTTAATGGCAGGACCACTTTGCGGACTTTATGGACTTTCTATTTTAATTGTTCAAAAGGTAAATCCTGC
TCCAAAAGATAAAGAAAGTG

Downstream 100 bases:

>100_bases
TTTTTCTTTAAAATTTATTGCTATTTTAGCTTGAAGTTTTAGCCAATCTTTTTGAAGCATGATAGGAAAACCTCCATAAA
CACGCCCGCCTTCTAAATTT

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA [H]

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MNKDLLLSSYDYTLANELIANYPTNPKEDARLLVFDRKNKEIFHTTFKNLQDFLPNCAIFFNDTKVIKARIYGNKASGGK
IELFLHQPFLNSHDPLFLAQIKGRVKKDEILYFKDDLKVRVVELLNDGLRKVQFFQNDKTLDTSNLYNLLDKIGHIPLPP
YIKREDEKSDLKDYQSIFAKNLGAVAAPTASLHFSETMLENLRKKHEIYHLTLHVGAGTFKSVECENIQEHKMHSEFFNI
PQQACEIIDSKQAILGVGTTVTRTIEYYARTKTKNGFCDLFLHPQNPPIRQNHLLTNFHLPKSTLIMLVSAFIGREQCLK
LYELAIKEKYRFYSYGDAMLIL

Sequences:

>Translated_342_residues
MNKDLLLSSYDYTLANELIANYPTNPKEDARLLVFDRKNKEIFHTTFKNLQDFLPNCAIFFNDTKVIKARIYGNKASGGK
IELFLHQPFLNSHDPLFLAQIKGRVKKDEILYFKDDLKVRVVELLNDGLRKVQFFQNDKTLDTSNLYNLLDKIGHIPLPP
YIKREDEKSDLKDYQSIFAKNLGAVAAPTASLHFSETMLENLRKKHEIYHLTLHVGAGTFKSVECENIQEHKMHSEFFNI
PQQACEIIDSKQAILGVGTTVTRTIEYYARTKTKNGFCDLFLHPQNPPIRQNHLLTNFHLPKSTLIMLVSAFIGREQCLK
LYELAIKEKYRFYSYGDAMLIL
>Mature_342_residues
MNKDLLLSSYDYTLANELIANYPTNPKEDARLLVFDRKNKEIFHTTFKNLQDFLPNCAIFFNDTKVIKARIYGNKASGGK
IELFLHQPFLNSHDPLFLAQIKGRVKKDEILYFKDDLKVRVVELLNDGLRKVQFFQNDKTLDTSNLYNLLDKIGHIPLPP
YIKREDEKSDLKDYQSIFAKNLGAVAAPTASLHFSETMLENLRKKHEIYHLTLHVGAGTFKSVECENIQEHKMHSEFFNI
PQQACEIIDSKQAILGVGTTVTRTIEYYARTKTKNGFCDLFLHPQNPPIRQNHLLTNFHLPKSTLIMLVSAFIGREQCLK
LYELAIKEKYRFYSYGDAMLIL

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA) [H]

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family [H]

Homologues:

Organism=Escherichia coli, GI1786606, Length=353, Percent_Identity=40.5099150141643, Blast_Score=245, Evalue=4e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003699 [H]

Pfam domain/function: PF02547 Queuosine_synth [H]

EC number: 5.-.-.- [C]

Molecular weight: Translated: 39570; Mature: 39570

Theoretical pI: Translated: 8.82; Mature: 8.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKDLLLSSYDYTLANELIANYPTNPKEDARLLVFDRKNKEIFHTTFKNLQDFLPNCAIF
CCCCEEEECCCHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCEEE
FNDTKVIKARIYGNKASGGKIELFLHQPFLNSHDPLFLAQIKGRVKKDEILYFKDDLKVR
ECCCEEEEEEEECCCCCCCEEEEEEECCCCCCCCCEEEEECCCCCCCCEEEEEECCCHHH
VVELLNDGLRKVQFFQNDKTLDTSNLYNLLDKIGHIPLPPYIKREDEKSDLKDYQSIFAK
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHH
NLGAVAAPTASLHFSETMLENLRKKHEIYHLTLHVGAGTFKSVECENIQEHKMHSEFFNI
CCCCCCCCCCHHHHHHHHHHHHHHHCEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHCC
PQQACEIIDSKQAILGVGTTVTRTIEYYARTKTKNGFCDLFLHPQNPPIRQNHLLTNFHL
HHHHHHHHCCCCCEEECCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCEEEEECC
PKSTLIMLVSAFIGREQCLKLYELAIKEKYRFYSYGDAMLIL
CHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHEEEECCEEEEC
>Mature Secondary Structure
MNKDLLLSSYDYTLANELIANYPTNPKEDARLLVFDRKNKEIFHTTFKNLQDFLPNCAIF
CCCCEEEECCCHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCEEE
FNDTKVIKARIYGNKASGGKIELFLHQPFLNSHDPLFLAQIKGRVKKDEILYFKDDLKVR
ECCCEEEEEEEECCCCCCCEEEEEEECCCCCCCCCEEEEECCCCCCCCEEEEEECCCHHH
VVELLNDGLRKVQFFQNDKTLDTSNLYNLLDKIGHIPLPPYIKREDEKSDLKDYQSIFAK
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHH
NLGAVAAPTASLHFSETMLENLRKKHEIYHLTLHVGAGTFKSVECENIQEHKMHSEFFNI
CCCCCCCCCCHHHHHHHHHHHHHHHCEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHCC
PQQACEIIDSKQAILGVGTTVTRTIEYYARTKTKNGFCDLFLHPQNPPIRQNHLLTNFHL
HHHHHHHHCCCCCEEECCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCEEEEECC
PKSTLIMLVSAFIGREQCLKLYELAIKEKYRFYSYGDAMLIL
CHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHEEEECCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA