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The map label for this gene is macA [H]

Identifier: 57238198

GI number: 57238198

Start: 640816

End: 641988

Strand: Direct

Name: macA [H]

Synonym: CJE0709

Alternate gene names: 57238198

Gene position: 640816-641988 (Clockwise)

Preceding gene: 57238199

Following gene: 57238197

Centisome position: 36.04

GC content: 31.88

Gene sequence:

>1173_bases
ATGAAAAAGAAGATTGTTTTAATCATTTTAATTGCAATACTTGGAAGTGTTGGGGCTTATTTTATTTTTTTTAATAATGA
TGAAAAAATCAGCTATTTAACTCAAAAAATACAAAAAAAAGATATATCTCAAACCATAGAGGCAGTAGGAAAGGTATATG
CCAAAGATCAAGTCGATGTGGGTGCTCAAGTTAGTGGACAAATTATAAAACTTTATGTTGATGTGGGAACTCATGTAAAG
CAAGGTGATTTGATCGCTCAAATTGATAAAGATAAACAACAAAACGATTTAGATATTACAAAAGCTCAGCTTGAAAGTGC
TAAGGCTAATTTAGAGAGTAAAAAAGTTGCCCTTGAGATTGCGAATAAGCAATATCAAAGAGAGCAAAAACTTTATGCAG
CTAAAGCAAGTTCTCTTGAAAATTTAGAAACTCAAAAAAATAATTATTATACTTTAAAAGCCAATGTTGCAGAGCTTAAT
GCTCAAGTAGTTCAGCTTGAAATCACTCTTAAAAATGCACAAAAAGATTTAGGTTATACAACCATTACTGCTCCTATGGA
TGGTGTTGTGATTAATGTAGCTGTAGATGAAGGACAAACAGTTAATGCTAATCAAAACACTCCTACTATAGTCCGTATAG
CTAATTTAGACGAAATGGAAGTAAGAATGGAAATAGCAGAGGCTGATGTGAGTAAGATAAAAGTAGGAACAGAGCTTGAT
TTTTCTTTGCTTAATGATCCTCAAAAAACTTATCATGCTAAGATTGCAAGTATAGATCCAGCTGATACTGAAGTGAGTGA
TTCTAGTACAAGCTCAAGCTCATCAAGTTCGAGTTCTTCAAGCTCAAGCTCATCAAATGCTATTTATTATTACGCAAAAT
TTTATGTAGCCAATAAAGATGATTTTTTGCGTATTGGTATGAGTATACAAAATGAAATTGTCGTAGCAAGTGCAAAGGCT
GTTTTAGCAGTGCCAACTTATGCAATTAAAAGCGATCCAAAAGGCTATTATGTTGAAATTTTGGAAAATCAAAAAGCTGT
TAAAAAATATGTCAAACTTGGCATTAAAGATTCTATCAATACTCAAATTTTAGAAGGTGTAAATGAAGATGAAGAATTGA
TAGTAAGCTCAAGTGCTGATGGTTTAGCTCCTAAAATGAAGTTGAGATTTTAA

Upstream 100 bases:

>100_bases
TTTAGAATTTTTAAATTTATAAAATAGAATTAAAAATTCACAAAAAATTCACAAAAAATGAGTAAAATTTAATCTTGATT
TTTCTTTAGGATTTGTTATT

Downstream 100 bases:

>100_bases
TGATTTTTCTTAAAAATATTTGCAAAAATATAGGCGAAAATGCCATTTTAAAAAATGTAAGTTTAAGTATAGAAAAAGGT
GAATTTGTTGCTATTATAGG

Product: macrolide-specific efflux protein macA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 390; Mature: 390

Protein sequence:

>390_residues
MKKKIVLIILIAILGSVGAYFIFFNNDEKISYLTQKIQKKDISQTIEAVGKVYAKDQVDVGAQVSGQIIKLYVDVGTHVK
QGDLIAQIDKDKQQNDLDITKAQLESAKANLESKKVALEIANKQYQREQKLYAAKASSLENLETQKNNYYTLKANVAELN
AQVVQLEITLKNAQKDLGYTTITAPMDGVVINVAVDEGQTVNANQNTPTIVRIANLDEMEVRMEIAEADVSKIKVGTELD
FSLLNDPQKTYHAKIASIDPADTEVSDSSTSSSSSSSSSSSSSSSNAIYYYAKFYVANKDDFLRIGMSIQNEIVVASAKA
VLAVPTYAIKSDPKGYYVEILENQKAVKKYVKLGIKDSINTQILEGVNEDEELIVSSSADGLAPKMKLRF

Sequences:

>Translated_390_residues
MKKKIVLIILIAILGSVGAYFIFFNNDEKISYLTQKIQKKDISQTIEAVGKVYAKDQVDVGAQVSGQIIKLYVDVGTHVK
QGDLIAQIDKDKQQNDLDITKAQLESAKANLESKKVALEIANKQYQREQKLYAAKASSLENLETQKNNYYTLKANVAELN
AQVVQLEITLKNAQKDLGYTTITAPMDGVVINVAVDEGQTVNANQNTPTIVRIANLDEMEVRMEIAEADVSKIKVGTELD
FSLLNDPQKTYHAKIASIDPADTEVSDSSTSSSSSSSSSSSSSSSNAIYYYAKFYVANKDDFLRIGMSIQNEIVVASAKA
VLAVPTYAIKSDPKGYYVEILENQKAVKKYVKLGIKDSINTQILEGVNEDEELIVSSSADGLAPKMKLRF
>Mature_390_residues
MKKKIVLIILIAILGSVGAYFIFFNNDEKISYLTQKIQKKDISQTIEAVGKVYAKDQVDVGAQVSGQIIKLYVDVGTHVK
QGDLIAQIDKDKQQNDLDITKAQLESAKANLESKKVALEIANKQYQREQKLYAAKASSLENLETQKNNYYTLKANVAELN
AQVVQLEITLKNAQKDLGYTTITAPMDGVVINVAVDEGQTVNANQNTPTIVRIANLDEMEVRMEIAEADVSKIKVGTELD
FSLLNDPQKTYHAKIASIDPADTEVSDSSTSSSSSSSSSSSSSSSNAIYYYAKFYVANKDDFLRIGMSIQNEIVVASAKA
VLAVPTYAIKSDPKGYYVEILENQKAVKKYVKLGIKDSINTQILEGVNEDEELIVSSSADGLAPKMKLRF

Specific function: Efflux transporter for macrolide antibiotics [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI87081796, Length=386, Percent_Identity=31.3471502590674, Blast_Score=194, Evalue=9e-51,
Organism=Escherichia coli, GI87082036, Length=339, Percent_Identity=25.0737463126844, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI87081951, Length=278, Percent_Identity=26.978417266187, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1790519, Length=241, Percent_Identity=26.5560165975104, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 42821; Mature: 42821

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKIVLIILIAILGSVGAYFIFFNNDEKISYLTQKIQKKDISQTIEAVGKVYAKDQVDV
CCCCEEHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GAQVSGQIIKLYVDVGTHVKQGDLIAQIDKDKQQNDLDITKAQLESAKANLESKKVALEI
CCCCCCEEEEEEEECCCCEECCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCHHEEEEEH
ANKQYQREQKLYAAKASSLENLETQKNNYYTLKANVAELNAQVVQLEITLKNAQKDLGYT
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEHHHCCEEEEEEEEEEECCCHHCCEE
TITAPMDGVVINVAVDEGQTVNANQNTPTIVRIANLDEMEVRMEIAEADVSKIKVGTELD
EEECCCCCEEEEEEECCCCEECCCCCCCEEEEEECCCCHHHEEHHHHCCHHHEEECCCCC
FSLLNDPQKTYHAKIASIDPADTEVSDSSTSSSSSSSSSSSSSSSNAIYYYAKFYVANKD
HHHCCCCCHHHEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEEECCC
DFLRIGMSIQNEIVVASAKAVLAVPTYAIKSDPKGYYVEILENQKAVKKYVKLGIKDSIN
CEEEECCCCCCEEEEEECCEEEEECCEEECCCCCCEEEEEECCCHHHHHHHHCCCCCCCC
TQILEGVNEDEELIVSSSADGLAPKMKLRF
HHHHHCCCCCCEEEEECCCCCCCCCEEECC
>Mature Secondary Structure
MKKKIVLIILIAILGSVGAYFIFFNNDEKISYLTQKIQKKDISQTIEAVGKVYAKDQVDV
CCCCEEHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GAQVSGQIIKLYVDVGTHVKQGDLIAQIDKDKQQNDLDITKAQLESAKANLESKKVALEI
CCCCCCEEEEEEEECCCCEECCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCHHEEEEEH
ANKQYQREQKLYAAKASSLENLETQKNNYYTLKANVAELNAQVVQLEITLKNAQKDLGYT
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEHHHCCEEEEEEEEEEECCCHHCCEE
TITAPMDGVVINVAVDEGQTVNANQNTPTIVRIANLDEMEVRMEIAEADVSKIKVGTELD
EEECCCCCEEEEEEECCCCEECCCCCCCEEEEEECCCCHHHEEHHHHCCHHHEEECCCCC
FSLLNDPQKTYHAKIASIDPADTEVSDSSTSSSSSSSSSSSSSSSNAIYYYAKFYVANKD
HHHCCCCCHHHEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEEECCC
DFLRIGMSIQNEIVVASAKAVLAVPTYAIKSDPKGYYVEILENQKAVKKYVKLGIKDSIN
CEEEECCCCCCEEEEEECCEEEEECCEEECCCCCCEEEEEECCCHHHHHHHHCCCCCCCC
TQILEGVNEDEELIVSSSADGLAPKMKLRF
HHHHHCCCCCCEEEEECCCCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]