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The map label for this gene is algI [H]

Identifier: 57238193

GI number: 57238193

Start: 647464

End: 648840

Strand: Reverse

Name: algI [H]

Synonym: CJE0714

Alternate gene names: 57238193

Gene position: 648840-647464 (Counterclockwise)

Preceding gene: 57238192

Following gene: 57238194

Centisome position: 36.5

GC content: 27.16

Gene sequence:

>1377_bases
ATGACTTATTTTTCTCTAGAATTTAGTATTTTAATGATAGCTTTTTTTGCTATTTATTGGACTTTTAAAAATAATTATAA
AATACAAAATATTTTAATTTTAATTTTCTCTTATATTATTTACATTTTAATCAATCCTTATTTCGCCTTGGTTTTATTTA
TATATACCTTTTTTATACACTATTTTGCCTTGCTAATTTTTGTGCGTCGCAAGCGTTATATTTTCGCTACTTGTATAGCT
TTTATTATTTTAAATTTATGTTTTTTTAAATACTTTCCTAGCATCAAAGGAAGTGTTGATGAGATATTAAATTTTTTCGG
TTTAGAATTTTTAAATATTGATTTAGTATTACCAATAGGAATTAGTTTTTATACCTTTACTTCTATCACTTATCTTGTGG
AAGTTTATCAAAAACGCCGTTTAGAAAGTTTTTTAAATTTAGCAACCTTCTTATCATTTTTTCCAACACTACTTTCAGGG
CCTATTATGCGAAGTTCTTTTTTCTTTGAACAGGCCTATCAAAAACGCGAATTTAAACATGCAAATTTAATCATCATCTT
GCTTGTTTTTGGTATAGTTAAAAAAGTTTTAATAGCCAATTACCTTGGAATTTATGCTAAAAGTATTTTAGACTTTCCAC
AATCTTATAATTTCATACAACTTTTAAGCGCAATTTACGCCTATGCAATTCAAATATACTGCGATTTTAGCGGATATGTT
GATTTAGTTTGCGCTTTTGCTTTAATGTTAGGTTTTACCCTTCCGCCTAACTTTAACATGCCTTATCTTGCAAAAAACCT
AAAAGATTTTTGGGCTAGATGGCATATCAGTCTTTCAACCTTTATAAGAGATTATATCTATATACCTTTAGGGGGCAATA
GAAAAGGAATGCCACGCACTATAGTTAACATACTCATCGCTTTTATACTTTCAGGTATGTGGCATGGTAATACACTTGCT
TTTATTGTTTGGGGTTTATTGCATGGTATTGGCATAGTTTTTATACATTTGCTAGCTTTAAGCAAATTTAGTTTACAAAA
AATTCCAGCTTTAGGAAGATTTTTAACCTTTCAATTTGTATGTTTTACTTGGATTTTCTTTTACTATAGCAAAAATCTAG
AAGATGCAATAGAATATTTTAAAGCTTGTTATTATAATTTCTTCCAAATTCCATCTTATAACGATATCTATATGTTGGTT
GCTTTTGGGGTTCTTTTTATGATCTATCCTTTATTTATTAATTTTAAAGAATATTGCATTAAGATTTTAAATCTTACTCC
ATTTTTGCTAAAACCTTTTATTATAGCTTTTATTTTACTTTTGGTTTTTGCTTTTATGCCAGATGGAATTCCTGATTTTA
TTTATTCGAGTTTTTAG

Upstream 100 bases:

>100_bases
TGGACTTTATTTAGCTGATCAATACATCAAAAATATTGCATTAAGTAGAAAAAAATAATCTTTGCCCTATTATGGGCAAA
TCTCGCTTTGGATTGAAGGT

Downstream 100 bases:

>100_bases
ATGAGTGTAGTTAGATTTTTCTTCATTTTGATTATAGTGCTTGGCTTGGTTGTTGTAGTTATGAATCAGAGCATTAGCTC
TTATATAGAACAAAAATATC

Product: alginate O-acetyltransferase AlgI

Products: NA

Alternate protein names: Alginate biosynthesis protein AlgI [H]

Number of amino acids: Translated: 458; Mature: 457

Protein sequence:

>458_residues
MTYFSLEFSILMIAFFAIYWTFKNNYKIQNILILIFSYIIYILINPYFALVLFIYTFFIHYFALLIFVRRKRYIFATCIA
FIILNLCFFKYFPSIKGSVDEILNFFGLEFLNIDLVLPIGISFYTFTSITYLVEVYQKRRLESFLNLATFLSFFPTLLSG
PIMRSSFFFEQAYQKREFKHANLIIILLVFGIVKKVLIANYLGIYAKSILDFPQSYNFIQLLSAIYAYAIQIYCDFSGYV
DLVCAFALMLGFTLPPNFNMPYLAKNLKDFWARWHISLSTFIRDYIYIPLGGNRKGMPRTIVNILIAFILSGMWHGNTLA
FIVWGLLHGIGIVFIHLLALSKFSLQKIPALGRFLTFQFVCFTWIFFYYSKNLEDAIEYFKACYYNFFQIPSYNDIYMLV
AFGVLFMIYPLFINFKEYCIKILNLTPFLLKPFIIAFILLLVFAFMPDGIPDFIYSSF

Sequences:

>Translated_458_residues
MTYFSLEFSILMIAFFAIYWTFKNNYKIQNILILIFSYIIYILINPYFALVLFIYTFFIHYFALLIFVRRKRYIFATCIA
FIILNLCFFKYFPSIKGSVDEILNFFGLEFLNIDLVLPIGISFYTFTSITYLVEVYQKRRLESFLNLATFLSFFPTLLSG
PIMRSSFFFEQAYQKREFKHANLIIILLVFGIVKKVLIANYLGIYAKSILDFPQSYNFIQLLSAIYAYAIQIYCDFSGYV
DLVCAFALMLGFTLPPNFNMPYLAKNLKDFWARWHISLSTFIRDYIYIPLGGNRKGMPRTIVNILIAFILSGMWHGNTLA
FIVWGLLHGIGIVFIHLLALSKFSLQKIPALGRFLTFQFVCFTWIFFYYSKNLEDAIEYFKACYYNFFQIPSYNDIYMLV
AFGVLFMIYPLFINFKEYCIKILNLTPFLLKPFIIAFILLLVFAFMPDGIPDFIYSSF
>Mature_457_residues
TYFSLEFSILMIAFFAIYWTFKNNYKIQNILILIFSYIIYILINPYFALVLFIYTFFIHYFALLIFVRRKRYIFATCIAF
IILNLCFFKYFPSIKGSVDEILNFFGLEFLNIDLVLPIGISFYTFTSITYLVEVYQKRRLESFLNLATFLSFFPTLLSGP
IMRSSFFFEQAYQKREFKHANLIIILLVFGIVKKVLIANYLGIYAKSILDFPQSYNFIQLLSAIYAYAIQIYCDFSGYVD
LVCAFALMLGFTLPPNFNMPYLAKNLKDFWARWHISLSTFIRDYIYIPLGGNRKGMPRTIVNILIAFILSGMWHGNTLAF
IVWGLLHGIGIVFIHLLALSKFSLQKIPALGRFLTFQFVCFTWIFFYYSKNLEDAIEYFKACYYNFFQIPSYNDIYMLVA
FGVLFMIYPLFINFKEYCIKILNLTPFLLKPFIIAFILLLVFAFMPDGIPDFIYSSF

Specific function: Together with AlgJ and AlgF, forms an inner membrane complex which probably interacts with the alginate polymerization- transport complex and adds acetyl groups at the O-2 and O-3 positions of polymannuronic acid. Acetylation of alginate is important for

COG id: COG1696

COG function: function code M; Predicted membrane protein involved in D-alanine export

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane-bound acyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004299 [H]

Pfam domain/function: PF03062 MBOAT [H]

EC number: NA

Molecular weight: Translated: 53976; Mature: 53845

Theoretical pI: Translated: 9.29; Mature: 9.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYFSLEFSILMIAFFAIYWTFKNNYKIQNILILIFSYIIYILINPYFALVLFIYTFFIH
CCEEEHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YFALLIFVRRKRYIFATCIAFIILNLCFFKYFPSIKGSVDEILNFFGLEFLNIDLVLPIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCHHHHCEEEEEECC
ISFYTFTSITYLVEVYQKRRLESFLNLATFLSFFPTLLSGPIMRSSFFFEQAYQKREFKH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
ANLIIILLVFGIVKKVLIANYLGIYAKSILDFPQSYNFIQLLSAIYAYAIQIYCDFSGYV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHEECCCHHH
DLVCAFALMLGFTLPPNFNMPYLAKNLKDFWARWHISLSTFIRDYIYIPLGGNRKGMPRT
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCHHH
IVNILIAFILSGMWHGNTLAFIVWGLLHGIGIVFIHLLALSKFSLQKIPALGRFLTFQFV
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CFTWIFFYYSKNLEDAIEYFKACYYNFFQIPSYNDIYMLVAFGVLFMIYPLFINFKEYCI
HHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
KILNLTPFLLKPFIIAFILLLVFAFMPDGIPDFIYSSF
HHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCC
>Mature Secondary Structure 
TYFSLEFSILMIAFFAIYWTFKNNYKIQNILILIFSYIIYILINPYFALVLFIYTFFIH
CEEEHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YFALLIFVRRKRYIFATCIAFIILNLCFFKYFPSIKGSVDEILNFFGLEFLNIDLVLPIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCHHHHCEEEEEECC
ISFYTFTSITYLVEVYQKRRLESFLNLATFLSFFPTLLSGPIMRSSFFFEQAYQKREFKH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
ANLIIILLVFGIVKKVLIANYLGIYAKSILDFPQSYNFIQLLSAIYAYAIQIYCDFSGYV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHEECCCHHH
DLVCAFALMLGFTLPPNFNMPYLAKNLKDFWARWHISLSTFIRDYIYIPLGGNRKGMPRT
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCHHH
IVNILIAFILSGMWHGNTLAFIVWGLLHGIGIVFIHLLALSKFSLQKIPALGRFLTFQFV
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CFTWIFFYYSKNLEDAIEYFKACYYNFFQIPSYNDIYMLVAFGVLFMIYPLFINFKEYCI
HHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
KILNLTPFLLKPFIIAFILLLVFAFMPDGIPDFIYSSF
HHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8636017; 10984043; 12003941; 11208804; 11179370 [H]