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The map label for this gene is cysK [H]

Identifier: 57237740

GI number: 57237740

Start: 926597

End: 927496

Strand: Reverse

Name: cysK [H]

Synonym: CJE0990

Alternate gene names: 57237740

Gene position: 927496-926597 (Counterclockwise)

Preceding gene: 57237741

Following gene: 57237735

Centisome position: 52.17

GC content: 31.44

Gene sequence:

>900_bases
ATGAAAGTATATGAAAAAGTAAGTGAGCTTATAGGAAATACTCCTATTATACATTTGAAAAAATTTGGTATCAATGTTTT
TGCTAAATGTGAATTTTTAAATCCTAGTCATTCTATAAAAGATAGAGCAGCTTTTGAAATGATTAAAGATGCTTTAGATA
GTAAAAAAATTAATCAAGATACTACAATAGTTGAAGCAACTAGTGGAAATACTGGAATTTCTTTGGCTATGATTTGTGCA
GATTTGGGACTTAAATTTATTGCTGTAATGCCCGAATCTATGAGTTTAGAAAGAAGAAAAATGATAACACTCTTTGGCGC
TAGGTTAGAATTAACTCCTGCAAATTTAGGAATGAAAGGAGCGGTTGATAAAGCAAATGAAATATTATTAAATACTCCAA
ATTCTTTTATGGTTTCTCAATTTGAAAATATTTCAAATAAAAATGCGCATCGTAAGAATACGGCTTTAGAAATTTTAAGA
GATTTGGATAATGAACTTGATATTTTTGTAGCTGGTTTTGGAACTGGCGGTACTATTAGTGGAGTTGGGGAAATACTAAA
AGAAAAATTAGAAAAAGTGCATATTGTTGGAGTCGAGCCTCTAAATTCTCCATTGCTTAGCAAAGGTGAAGCGGGAAGCC
ATAAAATACAAGGAATAGGGGCGAATTTTATACCTGCTATTTTAAATAAAGAAGTTATAGATGAAGTGATCACAGTAAGT
AATGAAGATGCTATAAATACAGCCAAAGAACTTGCCAAAAGTGGTTTAATGGTAGGTATATCAAGTGGAGCAAATGTATT
TGCCGCAAGTATGCTAGCAAAGAAATTTCCAGATAAAAGAATTTTAACTATGCTAAACGATACGGCTGAAAGATACCTCT
CAACTGATCTTTTTGCTTAA

Upstream 100 bases:

>100_bases
TCTTTCTTAATTAATCCAGATATTTTATATCTTTTATTTACTTTATTTTTTTAAACTTTTATAAAACTATCATTATTAAA
TAAAAAAGGATATTAAAATT

Downstream 100 bases:

>100_bases
GTATAATTATTGAGAATTTAGAAAAATTCTAAGTTCTCTTAAAATTTCTTGTGGATTTAAATCGCTAATTTCTTTATAAA
AATGACCTTTTTCATCAATA

Product: cysteine synthase A

Products: NA

Alternate protein names: CSase B; O-acetylserine (thiol)-lyase B; OAS-TL B; O-acetylserine sulfhydrylase B [H]

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MKVYEKVSELIGNTPIIHLKKFGINVFAKCEFLNPSHSIKDRAAFEMIKDALDSKKINQDTTIVEATSGNTGISLAMICA
DLGLKFIAVMPESMSLERRKMITLFGARLELTPANLGMKGAVDKANEILLNTPNSFMVSQFENISNKNAHRKNTALEILR
DLDNELDIFVAGFGTGGTISGVGEILKEKLEKVHIVGVEPLNSPLLSKGEAGSHKIQGIGANFIPAILNKEVIDEVITVS
NEDAINTAKELAKSGLMVGISSGANVFAASMLAKKFPDKRILTMLNDTAERYLSTDLFA

Sequences:

>Translated_299_residues
MKVYEKVSELIGNTPIIHLKKFGINVFAKCEFLNPSHSIKDRAAFEMIKDALDSKKINQDTTIVEATSGNTGISLAMICA
DLGLKFIAVMPESMSLERRKMITLFGARLELTPANLGMKGAVDKANEILLNTPNSFMVSQFENISNKNAHRKNTALEILR
DLDNELDIFVAGFGTGGTISGVGEILKEKLEKVHIVGVEPLNSPLLSKGEAGSHKIQGIGANFIPAILNKEVIDEVITVS
NEDAINTAKELAKSGLMVGISSGANVFAASMLAKKFPDKRILTMLNDTAERYLSTDLFA
>Mature_299_residues
MKVYEKVSELIGNTPIIHLKKFGINVFAKCEFLNPSHSIKDRAAFEMIKDALDSKKINQDTTIVEATSGNTGISLAMICA
DLGLKFIAVMPESMSLERRKMITLFGARLELTPANLGMKGAVDKANEILLNTPNSFMVSQFENISNKNAHRKNTALEILR
DLDNELDIFVAGFGTGGTISGVGEILKEKLEKVHIVGVEPLNSPLLSKGEAGSHKIQGIGANFIPAILNKEVIDEVITVS
NEDAINTAKELAKSGLMVGISSGANVFAASMLAKKFPDKRILTMLNDTAERYLSTDLFA

Specific function: Cysteine biosynthesis. [C]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=309, Percent_Identity=37.5404530744337, Blast_Score=195, Evalue=3e-50,
Organism=Homo sapiens, GI295821200, Length=309, Percent_Identity=37.5404530744337, Blast_Score=195, Evalue=3e-50,
Organism=Homo sapiens, GI4557415, Length=309, Percent_Identity=37.5404530744337, Blast_Score=195, Evalue=3e-50,
Organism=Escherichia coli, GI1788754, Length=311, Percent_Identity=44.6945337620579, Blast_Score=220, Evalue=1e-58,
Organism=Escherichia coli, GI2367138, Length=300, Percent_Identity=38.3333333333333, Blast_Score=193, Evalue=1e-50,
Organism=Caenorhabditis elegans, GI17535051, Length=305, Percent_Identity=44.5901639344262, Blast_Score=225, Evalue=2e-59,
Organism=Caenorhabditis elegans, GI17562970, Length=300, Percent_Identity=40, Blast_Score=209, Evalue=2e-54,
Organism=Caenorhabditis elegans, GI115535073, Length=302, Percent_Identity=43.3774834437086, Blast_Score=206, Evalue=1e-53,
Organism=Caenorhabditis elegans, GI32566674, Length=294, Percent_Identity=40.4761904761905, Blast_Score=204, Evalue=6e-53,
Organism=Caenorhabditis elegans, GI17561720, Length=300, Percent_Identity=40.6666666666667, Blast_Score=184, Evalue=4e-47,
Organism=Caenorhabditis elegans, GI32566672, Length=214, Percent_Identity=38.785046728972, Blast_Score=156, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17534315, Length=314, Percent_Identity=32.8025477707006, Blast_Score=131, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI25147552, Length=311, Percent_Identity=31.1897106109325, Blast_Score=119, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17561716, Length=118, Percent_Identity=42.3728813559322, Blast_Score=84, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17539640, Length=351, Percent_Identity=26.4957264957265, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI71996324, Length=322, Percent_Identity=26.3975155279503, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17539800, Length=334, Percent_Identity=25.4491017964072, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6321594, Length=314, Percent_Identity=35.6687898089172, Blast_Score=171, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6321449, Length=326, Percent_Identity=33.7423312883436, Blast_Score=129, Evalue=6e-31,
Organism=Drosophila melanogaster, GI24643623, Length=310, Percent_Identity=37.4193548387097, Blast_Score=184, Evalue=5e-47,
Organism=Drosophila melanogaster, GI20129101, Length=310, Percent_Identity=37.4193548387097, Blast_Score=184, Evalue=5e-47,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001216
- InterPro:   IPR005856
- InterPro:   IPR005859
- InterPro:   IPR001926
- InterPro:   IPR000634 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =2.5.1.47 [H]

Molecular weight: Translated: 32411; Mature: 32411

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: PS00165 DEHYDRATASE_SER_THR ; PS00901 CYS_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVYEKVSELIGNTPIIHLKKFGINVFAKCEFLNPSHSIKDRAAFEMIKDALDSKKINQD
CCHHHHHHHHHCCCCEEEEEECCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCC
TTIVEATSGNTGISLAMICADLGLKFIAVMPESMSLERRKMITLFGARLELTPANLGMKG
CEEEEECCCCCCHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHCCCEEEECCCCCCCCC
AVDKANEILLNTPNSFMVSQFENISNKNAHRKNTALEILRDLDNELDIFVAGFGTGGTIS
CCCCCCEEEEECCCHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCHH
GVGEILKEKLEKVHIVGVEPLNSPLLSKGEAGSHKIQGIGANFIPAILNKEVIDEVITVS
HHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCCCEEEECCCHHHHHHHCHHHHHHHHHCC
NEDAINTAKELAKSGLMVGISSGANVFAASMLAKKFPDKRILTMLNDTAERYLSTDLFA
CCHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKVYEKVSELIGNTPIIHLKKFGINVFAKCEFLNPSHSIKDRAAFEMIKDALDSKKINQD
CCHHHHHHHHHCCCCEEEEEECCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCC
TTIVEATSGNTGISLAMICADLGLKFIAVMPESMSLERRKMITLFGARLELTPANLGMKG
CEEEEECCCCCCHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHCCCEEEECCCCCCCCC
AVDKANEILLNTPNSFMVSQFENISNKNAHRKNTALEILRDLDNELDIFVAGFGTGGTIS
CCCCCCEEEEECCCHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCHH
GVGEILKEKLEKVHIVGVEPLNSPLLSKGEAGSHKIQGIGANFIPAILNKEVIDEVITVS
HHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCCCEEEECCCHHHHHHHCHHHHHHHHHCC
NEDAINTAKELAKSGLMVGISSGANVFAASMLAKKFPDKRILTMLNDTAERYLSTDLFA
CCHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9034314; 10688204 [H]