Click here to switch to the map view.

The map label for this gene is hemL [H]

Identifier: 57237694

GI number: 57237694

Start: 877458

End: 878732

Strand: Reverse

Name: hemL [H]

Synonym: CJE0940

Alternate gene names: 57237694

Gene position: 878732-877458 (Counterclockwise)

Preceding gene: 57237695

Following gene: 57237693

Centisome position: 49.43

GC content: 33.88

Gene sequence:

>1275_bases
ATGACAAACAAAAAAGCTTTTAAAGAAGCGTGTAAATTCATCGCAGGTGGTGTAAATTCTCCAGTTCGTGCTTTTGCAAA
TGTTCAAAGTGAGCCAAAATTTATTTCTCATGGAAAAGGTGCTTATATTTTCGATATAGATGGAAATTCTTATATAGACT
ATGTTCAAAGCTGGGGGCCTTTACTTTTTGGACATTGCGATAAAGATATACAAAAAGCTTGTCAAAAAGCACTTCATAAA
GGTTCAAGTTTTGGAGCTCCAACTTTATTAGAAACTGAACTTGCCAAGCTTGTTTTATCTGATTTTCCTCATTTAGAAAA
GATCCGCTTTGTAAGCAGTGGAACAGAAGCAACCATGAGTGCTATTCGTCTTGCCCGTGGTTTTACTAAAAAAGATAAAA
TTTTAAAATTTGAAGGATGTTATCACGGGCATTCTGATTCATTATTAGTGAGTGCAGGAAGTGGTGCTGCTACTTTTAAC
TCTCCTAGTTCTTTGGGTGTTTTAGAAGATGTAGCTAAACACACTTTAGTAGCTAAATATAATGATATAAATAGCGTTAA
AGAGCTTTTTGAAAAAAATAAAGATATTGCATGTGTTATCATTGAGCCTATTGCTGGAAATATGGGGTTAGTTCCAGCTA
AACAAGATTTTTTAGAAGAACTTGCTAAGATCTGTAAAAACAATCAAACTCTGCTGATTTTTGATGAAGTAATGAGTGGA
TATAGAGCATCTTACCTTGGTTCCTATGGAATCAATCATATCCAAGCAGATATAATTACTTTTGGTAAGGTAATAGGAGG
CGGTCTACCTGCAGCAGCATTTGCTTCTAGAGCTGAAATTATGGATATTTTAAGTCCTTTGGGTGGAGTTTATCAAGCAG
GAACTTTAAGTGGAAATCCTTTAGCAATGGCTGCAGGTATAGCAAGTCTTACAAAAGCCAAAAAGAAAACCAAGCTTTAT
GACAAACTTGGAAAATTAGCAAAAAAACTTACCCAAGGAATGAAAAAATTAGCAGATGAAAAAGGACTTCCACTGCAAGT
TTGTCATGTAGGTTCTATGTTTGGATACTTTTTCACCAAAGATCCTGTATCAAACTACCAAGATGCATTAAAATCCGATC
TAGCATTATTTTCAAAATTTCACAAAAATATGCTTGAAAATGGAATTTATTTAGCACCTTCTCAATTTGAAACAGGCTTT
ATATGTTCAAAAATGGATGATAAAATTATAGATACAACACTAGAGGCTGTTAGGGAAAGCTTTAAAAGAATATGA

Upstream 100 bases:

>100_bases
AGAGTAAAGACGCAAAATCAATCATGCCAAATTACTCTTTAACAAATGATGAAATTGTAACTTTATATAATTACATCAAG
CAATTTAGCAAGGAAGAAAA

Downstream 100 bases:

>100_bases
GCAATATTTTAAAAGAAGAAAAAAATCACTTAGAAAATTCCAACAGCAAAAGACAAAAAATCATTCGCAAAACTCTTGAA
GCTGCGGATGGTTTAAGTCT

Product: glutamate-1-semialdehyde aminotransferase

Products: NA

Alternate protein names: GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT [H]

Number of amino acids: Translated: 424; Mature: 423

Protein sequence:

>424_residues
MTNKKAFKEACKFIAGGVNSPVRAFANVQSEPKFISHGKGAYIFDIDGNSYIDYVQSWGPLLFGHCDKDIQKACQKALHK
GSSFGAPTLLETELAKLVLSDFPHLEKIRFVSSGTEATMSAIRLARGFTKKDKILKFEGCYHGHSDSLLVSAGSGAATFN
SPSSLGVLEDVAKHTLVAKYNDINSVKELFEKNKDIACVIIEPIAGNMGLVPAKQDFLEELAKICKNNQTLLIFDEVMSG
YRASYLGSYGINHIQADIITFGKVIGGGLPAAAFASRAEIMDILSPLGGVYQAGTLSGNPLAMAAGIASLTKAKKKTKLY
DKLGKLAKKLTQGMKKLADEKGLPLQVCHVGSMFGYFFTKDPVSNYQDALKSDLALFSKFHKNMLENGIYLAPSQFETGF
ICSKMDDKIIDTTLEAVRESFKRI

Sequences:

>Translated_424_residues
MTNKKAFKEACKFIAGGVNSPVRAFANVQSEPKFISHGKGAYIFDIDGNSYIDYVQSWGPLLFGHCDKDIQKACQKALHK
GSSFGAPTLLETELAKLVLSDFPHLEKIRFVSSGTEATMSAIRLARGFTKKDKILKFEGCYHGHSDSLLVSAGSGAATFN
SPSSLGVLEDVAKHTLVAKYNDINSVKELFEKNKDIACVIIEPIAGNMGLVPAKQDFLEELAKICKNNQTLLIFDEVMSG
YRASYLGSYGINHIQADIITFGKVIGGGLPAAAFASRAEIMDILSPLGGVYQAGTLSGNPLAMAAGIASLTKAKKKTKLY
DKLGKLAKKLTQGMKKLADEKGLPLQVCHVGSMFGYFFTKDPVSNYQDALKSDLALFSKFHKNMLENGIYLAPSQFETGF
ICSKMDDKIIDTTLEAVRESFKRI
>Mature_423_residues
TNKKAFKEACKFIAGGVNSPVRAFANVQSEPKFISHGKGAYIFDIDGNSYIDYVQSWGPLLFGHCDKDIQKACQKALHKG
SSFGAPTLLETELAKLVLSDFPHLEKIRFVSSGTEATMSAIRLARGFTKKDKILKFEGCYHGHSDSLLVSAGSGAATFNS
PSSLGVLEDVAKHTLVAKYNDINSVKELFEKNKDIACVIIEPIAGNMGLVPAKQDFLEELAKICKNNQTLLIFDEVMSGY
RASYLGSYGINHIQADIITFGKVIGGGLPAAAFASRAEIMDILSPLGGVYQAGTLSGNPLAMAAGIASLTKAKKKTKLYD
KLGKLAKKLTQGMKKLADEKGLPLQVCHVGSMFGYFFTKDPVSNYQDALKSDLALFSKFHKNMLENGIYLAPSQFETGFI
CSKMDDKIIDTTLEAVRESFKRI

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily [H]

Homologues:

Organism=Homo sapiens, GI13994255, Length=370, Percent_Identity=26.7567567567568, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI4557809, Length=328, Percent_Identity=26.219512195122, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI37574042, Length=363, Percent_Identity=26.7217630853994, Blast_Score=86, Evalue=7e-17,
Organism=Homo sapiens, GI226442705, Length=360, Percent_Identity=25.5555555555556, Blast_Score=76, Evalue=7e-14,
Organism=Homo sapiens, GI24119277, Length=381, Percent_Identity=23.8845144356955, Blast_Score=73, Evalue=5e-13,
Organism=Homo sapiens, GI226442709, Length=330, Percent_Identity=26.6666666666667, Blast_Score=72, Evalue=9e-13,
Organism=Homo sapiens, GI284507298, Length=240, Percent_Identity=26.25, Blast_Score=70, Evalue=4e-12,
Organism=Escherichia coli, GI1786349, Length=424, Percent_Identity=49.0566037735849, Blast_Score=427, Evalue=1e-121,
Organism=Escherichia coli, GI1789016, Length=385, Percent_Identity=27.7922077922078, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1788044, Length=285, Percent_Identity=32.9824561403509, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1789759, Length=335, Percent_Identity=28.3582089552239, Blast_Score=112, Evalue=7e-26,
Organism=Escherichia coli, GI1787560, Length=375, Percent_Identity=25.8666666666667, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI145693181, Length=341, Percent_Identity=26.9794721407625, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1786991, Length=311, Percent_Identity=25.4019292604502, Blast_Score=63, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI71992977, Length=383, Percent_Identity=27.154046997389, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI25144271, Length=301, Percent_Identity=26.9102990033223, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI25144274, Length=133, Percent_Identity=32.3308270676692, Blast_Score=69, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6323470, Length=392, Percent_Identity=28.5714285714286, Blast_Score=105, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6324432, Length=289, Percent_Identity=27.3356401384083, Blast_Score=88, Evalue=3e-18,
Organism=Drosophila melanogaster, GI21357415, Length=298, Percent_Identity=25.1677852348993, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21356575, Length=346, Percent_Identity=25.4335260115607, Blast_Score=89, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: =5.4.3.8 [H]

Molecular weight: Translated: 46091; Mature: 45960

Theoretical pI: Translated: 9.08; Mature: 9.08

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNKKAFKEACKFIAGGVNSPVRAFANVQSEPKFISHGKGAYIFDIDGNSYIDYVQSWGP
CCCHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCHHHCCCCEEEEECCCCHHHHHHHHCCC
LLFGHCDKDIQKACQKALHKGSSFGAPTLLETELAKLVLSDFPHLEKIRFVSSGTEATMS
CEECCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHH
AIRLARGFTKKDKILKFEGCYHGHSDSLLVSAGSGAATFNSPSSLGVLEDVAKHTLVAKY
HHHHHHCCCCCCCEEEEECCCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHH
NDINSVKELFEKNKDIACVIIEPIAGNMGLVPAKQDFLEELAKICKNNQTLLIFDEVMSG
CCHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEHHHHCC
YRASYLGSYGINHIQADIITFGKVIGGGLPAAAFASRAEIMDILSPLGGVYQAGTLSGNP
HHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHCCCCCCCCC
LAMAAGIASLTKAKKKTKLYDKLGKLAKKLTQGMKKLADEKGLPLQVCHVGSMFGYFFTK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHCC
DPVSNYQDALKSDLALFSKFHKNMLENGIYLAPSQFETGFICSKMDDKIIDTTLEAVRES
CCHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCHHHHHCHHHHHHHHHHHHHH
FKRI
HHCC
>Mature Secondary Structure 
TNKKAFKEACKFIAGGVNSPVRAFANVQSEPKFISHGKGAYIFDIDGNSYIDYVQSWGP
CCHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCHHHCCCCEEEEECCCCHHHHHHHHCCC
LLFGHCDKDIQKACQKALHKGSSFGAPTLLETELAKLVLSDFPHLEKIRFVSSGTEATMS
CEECCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHH
AIRLARGFTKKDKILKFEGCYHGHSDSLLVSAGSGAATFNSPSSLGVLEDVAKHTLVAKY
HHHHHHCCCCCCCEEEEECCCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHH
NDINSVKELFEKNKDIACVIIEPIAGNMGLVPAKQDFLEELAKICKNNQTLLIFDEVMSG
CCHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEHHHHCC
YRASYLGSYGINHIQADIITFGKVIGGGLPAAAFASRAEIMDILSPLGGVYQAGTLSGNP
HHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHCCCCCCCCC
LAMAAGIASLTKAKKKTKLYDKLGKLAKKLTQGMKKLADEKGLPLQVCHVGSMFGYFFTK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHCC
DPVSNYQDALKSDLALFSKFHKNMLENGIYLAPSQFETGFICSKMDDKIIDTTLEAVRES
CCHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCHHHHHCHHHHHHHHHHHHHH
FKRI
HHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA