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The map label for this gene is slt [C]

Identifier: 57237684

GI number: 57237684

Start: 867877

End: 869502

Strand: Reverse

Name: slt [C]

Synonym: CJE0930

Alternate gene names: 57237684

Gene position: 869502-867877 (Counterclockwise)

Preceding gene: 57237685

Following gene: 57237682

Centisome position: 48.91

GC content: 27.92

Gene sequence:

>1626_bases
GTGATAAAAAAAATACTCGTTTTTTTATTTATTTTTTCAAGTTTAAATGCTACACAATATAGTATAGAAAAACTCAAAAA
GGAAGAAAATTCACTTGCTAAAGATTATTATATTTATAGGCTTTTAGAAAAAAATAAAATCAGTAAAAAAGATGCACAGG
ATTTAAATTCTCATATTTTTCGCTATATAGGAAAAATTAAATCCGAACTTGAAAAAATCATCCCTTTAAAGCCTTATATC
AACCCAAAATATGCTAAATGCTATACCTATACAGCAAATACTATCTTAGATGCAAATTTAACTTGTCAAAGTGTGCGCTT
AAATTCATTGGTTTTTATAGCGAGTCTAAATTCCAAAGACCGAACTACTTTAGCTCAAACCTTTAAAAATCAAAGACCTG
ATTTGACAAATTTATTGCTAGCTTTTAATACTTCTGATCCTATGAGCTATATAGTACAAAAAGAAGATATTAATGGTTTT
TTTAAACTTTATAATTATTCTAAAAAATACGACTTAGATTTAAATACAAGTTTAGTCAATAAACTACCAAACCACATTGG
TTTTAAAGATTTTGCACAAAATATTATTATTAAAAAAGAAAATCCAAAATTTAGATATTCTATGCTAGAAATAAATCCTG
AAAATGTAAGTGAAGATTCTGCTTTCTATTTAGGAGTCAATGCCTTAACTTATGATAAAACAGAGCTTGCTTATGACTTT
TTTAAAAAGGCGGCACAAAGTTTTAAATCGCAAAGTAATAAAGACAATGCTATATTTTGGATGTGGTTGATTAAAAACAA
TGAAGAAGATTTAAAAACTTTATCGCAAAGTTCTTCTTTAAATATTTATAGTCTTTACGCAAAAGAACTTACTAACACAC
CTTTTCCAAAGATAGAAAGCCTTAATCCAAGCAAGAAAAAAAATAATTTTAATATGCAAGATCCTTTTGCTTGGCAAAAA
ATAAACAAACAAATTCGCGATGCTAACGCAAGTCAACTAGATGTCTTGGCAAAAGAATTTGACACACAAGAAACCTTGCC
TATTTATGCTTACATATTAGAACGCAAAAATAATTTTAAAAAACATTATTTTATAATGCCTTATTATGACAATATAAAAG
ACTATAACAAAACAAGACAAGCTTTAATTTTGGCTATTGCCAGACAAGAAAGTCGTTTTATTCCAACTGCTATTTCAGTT
TCTTATGCTTTAGGCATGATGCAATTTATGCCATTTTTAGCCAATCACATAGGGGAAAAAGAATTAAAAATTCCAAATTT
CGATCAAGATTTTATGTTTAAACCTGAAATAGCATATTACTTTGGAAACTATCATCTAAATTACCTTGAATCACGTTTAA
AATCTCCACTTTTTGTCGCTTATGCTTATAATGGCGGTATAGGATTTACTAACCGTATGCTTGCAAGAAATGATATGTTT
AAGACAGGCAAATTTGAACCTTTCTTGTCTATGGAGCTAGTTCCTTATCAAGAAAGCCGAATTTACGGAAAAAAAGTTCT
AGCTAATTATATTGTGTATCGACATCTTCTGAACGATAGTATAAAGATTTCGGATATTTTTGAAAATTTAATTCAAAACA
AGGCAAATGATCTAAACAAATCTTAG

Upstream 100 bases:

>100_bases
TTCCAACACGCATTGGCAATATTGATCTTGCGCCTTTGATTATAGTCTTAGCATTGCAGTTTCTTGGCATTTTTCTTGGG
AATATCTTAAGGAGTATATT

Downstream 100 bases:

>100_bases
CTACAAGCTTAGTCTCTTCCTTGACACTAGGAAGAGATACTTTAAAATAATCAGTATAGTTGTTTTGTATTATATATTTT
AAATACTCATCATCTAATTT

Product: soluble lytic murein transglycosylase

Products: 1,6-Anhydrobond [C]

Alternate protein names: Soluble Lytic Murein Transglycosylase; Lytic Murein Transglycosylase; Secreted Transglycosylase; Transglycosylase SLT Domain Protein; Transglycosylase SLT Domain-Containing Protein; Slt Family Transglycosylase; Soluble Lytic Murein Transglycosylase SLT Family; Murein Transglycosylase Domain-Containing Protein; Lytic Transglycosylase; SLT Domain-Containing Protein

Number of amino acids: Translated: 541; Mature: 541

Protein sequence:

>541_residues
MIKKILVFLFIFSSLNATQYSIEKLKKEENSLAKDYYIYRLLEKNKISKKDAQDLNSHIFRYIGKIKSELEKIIPLKPYI
NPKYAKCYTYTANTILDANLTCQSVRLNSLVFIASLNSKDRTTLAQTFKNQRPDLTNLLLAFNTSDPMSYIVQKEDINGF
FKLYNYSKKYDLDLNTSLVNKLPNHIGFKDFAQNIIIKKENPKFRYSMLEINPENVSEDSAFYLGVNALTYDKTELAYDF
FKKAAQSFKSQSNKDNAIFWMWLIKNNEEDLKTLSQSSSLNIYSLYAKELTNTPFPKIESLNPSKKKNNFNMQDPFAWQK
INKQIRDANASQLDVLAKEFDTQETLPIYAYILERKNNFKKHYFIMPYYDNIKDYNKTRQALILAIARQESRFIPTAISV
SYALGMMQFMPFLANHIGEKELKIPNFDQDFMFKPEIAYYFGNYHLNYLESRLKSPLFVAYAYNGGIGFTNRMLARNDMF
KTGKFEPFLSMELVPYQESRIYGKKVLANYIVYRHLLNDSIKISDIFENLIQNKANDLNKS

Sequences:

>Translated_541_residues
MIKKILVFLFIFSSLNATQYSIEKLKKEENSLAKDYYIYRLLEKNKISKKDAQDLNSHIFRYIGKIKSELEKIIPLKPYI
NPKYAKCYTYTANTILDANLTCQSVRLNSLVFIASLNSKDRTTLAQTFKNQRPDLTNLLLAFNTSDPMSYIVQKEDINGF
FKLYNYSKKYDLDLNTSLVNKLPNHIGFKDFAQNIIIKKENPKFRYSMLEINPENVSEDSAFYLGVNALTYDKTELAYDF
FKKAAQSFKSQSNKDNAIFWMWLIKNNEEDLKTLSQSSSLNIYSLYAKELTNTPFPKIESLNPSKKKNNFNMQDPFAWQK
INKQIRDANASQLDVLAKEFDTQETLPIYAYILERKNNFKKHYFIMPYYDNIKDYNKTRQALILAIARQESRFIPTAISV
SYALGMMQFMPFLANHIGEKELKIPNFDQDFMFKPEIAYYFGNYHLNYLESRLKSPLFVAYAYNGGIGFTNRMLARNDMF
KTGKFEPFLSMELVPYQESRIYGKKVLANYIVYRHLLNDSIKISDIFENLIQNKANDLNKS
>Mature_541_residues
MIKKILVFLFIFSSLNATQYSIEKLKKEENSLAKDYYIYRLLEKNKISKKDAQDLNSHIFRYIGKIKSELEKIIPLKPYI
NPKYAKCYTYTANTILDANLTCQSVRLNSLVFIASLNSKDRTTLAQTFKNQRPDLTNLLLAFNTSDPMSYIVQKEDINGF
FKLYNYSKKYDLDLNTSLVNKLPNHIGFKDFAQNIIIKKENPKFRYSMLEINPENVSEDSAFYLGVNALTYDKTELAYDF
FKKAAQSFKSQSNKDNAIFWMWLIKNNEEDLKTLSQSSSLNIYSLYAKELTNTPFPKIESLNPSKKKNNFNMQDPFAWQK
INKQIRDANASQLDVLAKEFDTQETLPIYAYILERKNNFKKHYFIMPYYDNIKDYNKTRQALILAIARQESRFIPTAISV
SYALGMMQFMPFLANHIGEKELKIPNFDQDFMFKPEIAYYFGNYHLNYLESRLKSPLFVAYAYNGGIGFTNRMLARNDMF
KTGKFEPFLSMELVPYQESRIYGKKVLANYIVYRHLLNDSIKISDIFENLIQNKANDLNKS

Specific function: Murein-Degrading Enzyme. Catalyzes The Cleavage Of The Glycosidic Bonds Between N-Acetylmuramic Acid And N- Acetylglucosamine Residues In Peptidoglycan. May Play A Role In Recycling Of Muropeptides During Cell Elongation And/Or Cell Division. [C]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasmic Protein. Tightly Associated With The Murein Sacculus [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082441, Length=179, Percent_Identity=30.1675977653631, Blast_Score=69, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 63358; Mature: 63358

Theoretical pI: Translated: 9.72; Mature: 9.72

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKKILVFLFIFSSLNATQYSIEKLKKEENSLAKDYYIYRLLEKNKISKKDAQDLNSHIF
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
RYIGKIKSELEKIIPLKPYINPKYAKCYTYTANTILDANLTCQSVRLNSLVFIASLNSKD
HHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCEEEECCCEEEEEEECEEEEEEECCCCC
RTTLAQTFKNQRPDLTNLLLAFNTSDPMSYIVQKEDINGFFKLYNYSKKYDLDLNTSLVN
HHHHHHHHHCCCCCHHHEEEEECCCCCHHHHEECCCCCHHHHHHCCCCEECCCCCHHHHH
KLPNHIGFKDFAQNIIIKKENPKFRYSMLEINPENVSEDSAFYLGVNALTYDKTELAYDF
HCHHHCCHHHHHCCEEEEECCCCEEEEEEEECCCCCCCCCEEEEEEEEEECCHHHHHHHH
FKKAAQSFKSQSNKDNAIFWMWLIKNNEEDLKTLSQSSSLNIYSLYAKELTNTPFPKIES
HHHHHHHHHHCCCCCCEEEEEEEEECCHHHHHHHHCCCCCEEEEEEHHHHCCCCCCCCCC
LNPSKKKNNFNMQDPFAWQKINKQIRDANASQLDVLAKEFDTQETLPIYAYILERKNNFK
CCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHCCCCC
KHYFIMPYYDNIKDYNKTRQALILAIARQESRFIPTAISVSYALGMMQFMPFLANHIGEK
EEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
ELKIPNFDQDFMFKPEIAYYFGNYHLNYLESRLKSPLFVAYAYNGGIGFTNRMLARNDMF
CCCCCCCCCCCEECCCEEEEECCCHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHCCCC
KTGKFEPFLSMELVPYQESRIYGKKVLANYIVYRHLLNDSIKISDIFENLIQNKANDLNK
CCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCC
S
C
>Mature Secondary Structure
MIKKILVFLFIFSSLNATQYSIEKLKKEENSLAKDYYIYRLLEKNKISKKDAQDLNSHIF
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
RYIGKIKSELEKIIPLKPYINPKYAKCYTYTANTILDANLTCQSVRLNSLVFIASLNSKD
HHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCEEEECCCEEEEEEECEEEEEEECCCCC
RTTLAQTFKNQRPDLTNLLLAFNTSDPMSYIVQKEDINGFFKLYNYSKKYDLDLNTSLVN
HHHHHHHHHCCCCCHHHEEEEECCCCCHHHHEECCCCCHHHHHHCCCCEECCCCCHHHHH
KLPNHIGFKDFAQNIIIKKENPKFRYSMLEINPENVSEDSAFYLGVNALTYDKTELAYDF
HCHHHCCHHHHHCCEEEEECCCCEEEEEEEECCCCCCCCCEEEEEEEEEECCHHHHHHHH
FKKAAQSFKSQSNKDNAIFWMWLIKNNEEDLKTLSQSSSLNIYSLYAKELTNTPFPKIES
HHHHHHHHHHCCCCCCEEEEEEEEECCHHHHHHHHCCCCCEEEEEEHHHHCCCCCCCCCC
LNPSKKKNNFNMQDPFAWQKINKQIRDANASQLDVLAKEFDTQETLPIYAYILERKNNFK
CCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHCCCCC
KHYFIMPYYDNIKDYNKTRQALILAIARQESRFIPTAISVSYALGMMQFMPFLANHIGEK
EEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
ELKIPNFDQDFMFKPEIAYYFGNYHLNYLESRLKSPLFVAYAYNGGIGFTNRMLARNDMF
CCCCCCCCCCCEECCCEEEEECCCHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHCCCC
KTGKFEPFLSMELVPYQESRIYGKKVLANYIVYRHLLNDSIKISDIFENLIQNKANDLNK
CCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCC
S
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA