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The map label for this gene is dpnA [H]

Identifier: 57237552

GI number: 57237552

Start: 502374

End: 503126

Strand: Reverse

Name: dpnA [H]

Synonym: CJE0550

Alternate gene names: 57237552

Gene position: 503126-502374 (Counterclockwise)

Preceding gene: 57237553

Following gene: 57237551

Centisome position: 28.3

GC content: 29.48

Gene sequence:

>753_bases
ATGAAACCAAATTTATATAACGACCATTTTCAAAATTTTAAAAGATATAATATACCAAAAGCACAGCTTATAATAGCTGA
TATTCCTTATAATTTAGGCAACAATGCTTATGCTTCATCTCCTGAATGGTATATAAATGGGGATAATAAAAATGGAGAAA
GCAAAAAAGCAAACAAGGCGTTTTTTGATACAGATAATGATTTTAGAGTTAGCGAATTTATGCACTTTTGCTCTAAAATG
CTTATAAAAGAACCTAAAGAATGCGGTAAAAGTCCTTGCATGATTGTTTTTTGCTCTTTTGAACAACAAGCAATGTTAAT
TGAAGTAGCTAAAAAATATGGCTTTAATCATTGTATAAATTTGGTTTTTAGAAAACAAAGCTCATCTCAAGTTTTAAAAG
CAAATATGAAAATAGTTGGAAATTGTGAATATGCTTTAATCTTATATCGTGAAAAACTTCCAAAATTTAACAATGATGGA
AAGATGATTTATAACTGCATGGATTGGCAAAAAGATGAAGGTATTCCTAAAGTACATCCCACACAAAAGCCTGTTAAATT
ACTAGAAAGATTAATCACTATTTTTACAGATGCAGGCGATGTTGTTATAGATCCATGTGCTGGAAGCGGAAGCACTCTTT
TAGCAGCCTGTAATTTAAACCGCAAAGCTTATGGCTTTGAGATTAAAAAAGACTTTTTTAAAAGTGCTAATGAAATTATG
TTTAAACATATAGAAAGAAGTCTATTTGCTTAA

Upstream 100 bases:

>100_bases
ATAAACGATATTTGCAGTTTTGCTCTAAATCCTTCTCAAAGAAATTACAACGCTTTAATAAATAGTCTTAAAAAATATAT
TAGAGAAAGAAAGAATGCTA

Downstream 100 bases:

>100_bases
GTAAAGTTTTGGTAAAATTATCAAATTAAAGAAAGGAAAAATTATGAATGAAAAAGACTTTATTGAAATAAGTAAAAAAT
TTATGGATGAACATCCAACA

Product: site-specific DNA-methyltransferase

Products: NA

Alternate protein names: M.DpnIIB; Adenine-specific methyltransferase DpnIIB; M.DpnII 2 [H]

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MKPNLYNDHFQNFKRYNIPKAQLIIADIPYNLGNNAYASSPEWYINGDNKNGESKKANKAFFDTDNDFRVSEFMHFCSKM
LIKEPKECGKSPCMIVFCSFEQQAMLIEVAKKYGFNHCINLVFRKQSSSQVLKANMKIVGNCEYALILYREKLPKFNNDG
KMIYNCMDWQKDEGIPKVHPTQKPVKLLERLITIFTDAGDVVIDPCAGSGSTLLAACNLNRKAYGFEIKKDFFKSANEIM
FKHIERSLFA

Sequences:

>Translated_250_residues
MKPNLYNDHFQNFKRYNIPKAQLIIADIPYNLGNNAYASSPEWYINGDNKNGESKKANKAFFDTDNDFRVSEFMHFCSKM
LIKEPKECGKSPCMIVFCSFEQQAMLIEVAKKYGFNHCINLVFRKQSSSQVLKANMKIVGNCEYALILYREKLPKFNNDG
KMIYNCMDWQKDEGIPKVHPTQKPVKLLERLITIFTDAGDVVIDPCAGSGSTLLAACNLNRKAYGFEIKKDFFKSANEIM
FKHIERSLFA
>Mature_250_residues
MKPNLYNDHFQNFKRYNIPKAQLIIADIPYNLGNNAYASSPEWYINGDNKNGESKKANKAFFDTDNDFRVSEFMHFCSKM
LIKEPKECGKSPCMIVFCSFEQQAMLIEVAKKYGFNHCINLVFRKQSSSQVLKANMKIVGNCEYALILYREKLPKFNNDG
KMIYNCMDWQKDEGIPKVHPTQKPVKLLERLITIFTDAGDVVIDPCAGSGSTLLAACNLNRKAYGFEIKKDFFKSANEIM
FKHIERSLFA

Specific function: Essential for establishment of a previously unmethylated plasmid transformed into the cell as single stranded DNA. Recognizes the single- or double-stranded sequence GATC, causes specific methylation on A-2 on one or both strands (respectively), and prote

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI87082238, Length=144, Percent_Identity=34.0277777777778, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002941
- InterPro:   IPR002052
- InterPro:   IPR001091 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 28739; Mature: 28739

Theoretical pI: Translated: 8.90; Mature: 8.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
7.2 %Cys+Met (Translated Protein)
3.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
7.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPNLYNDHFQNFKRYNIPKAQLIIADIPYNLGNNAYASSPEWYINGDNKNGESKKANKA
CCCCCCCHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCC
FFDTDNDFRVSEFMHFCSKMLIKEPKECGKSPCMIVFCSFEQQAMLIEVAKKYGFNHCIN
CCCCCCCEEHHHHHHHHHHHHHCCHHHHCCCCEEEEEECCCCHHHHHHHHHHHCHHHHHH
LVFRKQSSSQVLKANMKIVGNCEYALILYREKLPKFNNDGKMIYNCMDWQKDEGIPKVHP
HHHCCCCCHHHHHCCCEEEECCCEEEEEEEHHCCCCCCCCCEEEEECCCCCCCCCCCCCC
TQKPVKLLERLITIFTDAGDVVIDPCAGSGSTLLAACNLNRKAYGFEIKKDFFKSANEIM
CHHHHHHHHHHHHHHCCCCCEEEECCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHH
FKHIERSLFA
HHHHHHHHCC
>Mature Secondary Structure
MKPNLYNDHFQNFKRYNIPKAQLIIADIPYNLGNNAYASSPEWYINGDNKNGESKKANKA
CCCCCCCHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCC
FFDTDNDFRVSEFMHFCSKMLIKEPKECGKSPCMIVFCSFEQQAMLIEVAKKYGFNHCIN
CCCCCCCEEHHHHHHHHHHHHHCCHHHHCCCCEEEEEECCCCHHHHHHHHHHHCHHHHHH
LVFRKQSSSQVLKANMKIVGNCEYALILYREKLPKFNNDGKMIYNCMDWQKDEGIPKVHP
HHHCCCCCHHHHHCCCEEEECCCEEEEEEEHHCCCCCCCCCEEEEECCCCCCCCCCCCCC
TQKPVKLLERLITIFTDAGDVVIDPCAGSGSTLLAACNLNRKAYGFEIKKDFFKSANEIM
CHHHHHHHHHHHHHHCCCCCEEEECCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHH
FKHIERSLFA
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3019562; 10712690; 2824782 [H]