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The map label for this gene is tuf [H]

Identifier: 57237524

GI number: 57237524

Start: 472565

End: 473764

Strand: Direct

Name: tuf [H]

Synonym: CJE0520

Alternate gene names: 57237524

Gene position: 472565-473764 (Clockwise)

Preceding gene: 57237523

Following gene: 57237525

Centisome position: 26.58

GC content: 36.75

Gene sequence:

>1200_bases
ATGGCTAAAGAAAAATTTTCACGTAATAAGCCACACGTAAATATTGGTACTATTGGTCATGTTGACCATGGTAAAACTAC
TTTAACAGCTGCTATTTCTGCTGTTCTTTCTAGAAGAGGTTTAGCAGAGCTTAAAGATTATGATAATATCGATAATGCTC
CAGAAGAAAAAGAGCGTGGTATTACTATTGCTACTTCTCATATTGAATATGAAACAGACAATCGTCACTATGCACACGTT
GACTGCCCAGGTCACGCAGATTATGTTAAAAACATGATTACAGGTGCTGCACAAATGGATGGAGCGATCTTGGTTGTTTC
TGCTGCAGATGGCCCTATGCCACAAACTAGAGAGCACATTCTTCTTTCTCGTCAAGTAGGCGTTCCATATATTGTTGTTT
TTATGAATAAAGCAGATATGGTTGATGATGCTGAACTTTTAGAGTTAGTTGAAATGGAAATTAGAGAATTATTAAGCTCT
TATGATTTCCCAGGCGATGATACACCTATTATTTCTGGTTCTGCTTTAAAAGCTCTTGAAGAAGCTAAAGCTGGACAAGA
TGGTGAATGGTCAGCAAAAATTATGGATCTTATGGCTGCAGTTGATAGCTATATTCCAACTCCAACTCGTGATACTGAAA
AAGACTTCTTGATGCCAATTGAAGACGTTTTCTCAATTTCAGGTCGTGGTACTGTTGTTACAGGTAGAATTGAAAAAGGT
GTTGTAAAAGTAGGTGATACTATCGAAATCGTTGGTATTAAAGATACTCAAACAACAACTGTAACAGGTGTTGAAATGTT
CAGAAAAGAAATGGATCAAGGCGAAGCAGGAGATAACGTAGGTGTTCTTCTTCGTGGTACTAAAAAAGAAGAAGTTATCC
GTGGTATGGTTCTTGCTAAACCAAAATCAATTACTCCACACACTGACTTCGAAGCTGAAGTTTATATCTTAAATAAAGAT
GAAGGTGGTAGACATACTCCATTCTTTAACAACTATAGACCACAGTTTTATGTAAGAACAACTGATGTTACAGGTTCGAT
TAAATTAGCTGATGGTGTTGAAATGGTTATGCCAGGTGAAAATGTGAGAATTACTGTAAGCTTGATCGCTCCAGTAGCAC
TTGAAGAAGGAACTCGTTTTGCTATTCGTGAAGGTGGTAAAACTGTTGGTTCAGGTGTTGTTTCTAAAATTATTAAATAA

Upstream 100 bases:

>100_bases
GGTCGCGGGTTCAATTCCCGCTATGAGCTCCATGAATTGAAAAAATTATATAGATATAAGACTTGCTTTGCTCAATTTAT
TTTTTATACGGAGGAAAAAA

Downstream 100 bases:

>100_bases
TTTAATAAAGCCAAGTTAAAAGCTTGGCTTTAATTTTTTAAGGAAAATTGAAATGAGAATTAAAGTTGGTTTAAAGTGTG
AAGAATGTGGTGATATTAAT

Product: elongation factor Tu

Products: NA

Alternate protein names: EF-Tu [H]

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MAKEKFSRNKPHVNIGTIGHVDHGKTTLTAAISAVLSRRGLAELKDYDNIDNAPEEKERGITIATSHIEYETDNRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLSRQVGVPYIVVFMNKADMVDDAELLELVEMEIRELLSS
YDFPGDDTPIISGSALKALEEAKAGQDGEWSAKIMDLMAAVDSYIPTPTRDTEKDFLMPIEDVFSISGRGTVVTGRIEKG
VVKVGDTIEIVGIKDTQTTTVTGVEMFRKEMDQGEAGDNVGVLLRGTKKEEVIRGMVLAKPKSITPHTDFEAEVYILNKD
EGGRHTPFFNNYRPQFYVRTTDVTGSIKLADGVEMVMPGENVRITVSLIAPVALEEGTRFAIREGGKTVGSGVVSKIIK

Sequences:

>Translated_399_residues
MAKEKFSRNKPHVNIGTIGHVDHGKTTLTAAISAVLSRRGLAELKDYDNIDNAPEEKERGITIATSHIEYETDNRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLSRQVGVPYIVVFMNKADMVDDAELLELVEMEIRELLSS
YDFPGDDTPIISGSALKALEEAKAGQDGEWSAKIMDLMAAVDSYIPTPTRDTEKDFLMPIEDVFSISGRGTVVTGRIEKG
VVKVGDTIEIVGIKDTQTTTVTGVEMFRKEMDQGEAGDNVGVLLRGTKKEEVIRGMVLAKPKSITPHTDFEAEVYILNKD
EGGRHTPFFNNYRPQFYVRTTDVTGSIKLADGVEMVMPGENVRITVSLIAPVALEEGTRFAIREGGKTVGSGVVSKIIK
>Mature_398_residues
AKEKFSRNKPHVNIGTIGHVDHGKTTLTAAISAVLSRRGLAELKDYDNIDNAPEEKERGITIATSHIEYETDNRHYAHVD
CPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLSRQVGVPYIVVFMNKADMVDDAELLELVEMEIRELLSSY
DFPGDDTPIISGSALKALEEAKAGQDGEWSAKIMDLMAAVDSYIPTPTRDTEKDFLMPIEDVFSISGRGTVVTGRIEKGV
VKVGDTIEIVGIKDTQTTTVTGVEMFRKEMDQGEAGDNVGVLLRGTKKEEVIRGMVLAKPKSITPHTDFEAEVYILNKDE
GGRHTPFFNNYRPQFYVRTTDVTGSIKLADGVEMVMPGENVRITVSLIAPVALEEGTRFAIREGGKTVGSGVVSKIIK

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=398, Percent_Identity=55.7788944723618, Blast_Score=476, Evalue=1e-134,
Organism=Homo sapiens, GI223555963, Length=440, Percent_Identity=29.5454545454545, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI5729864, Length=440, Percent_Identity=29.5454545454545, Blast_Score=170, Evalue=3e-42,
Organism=Homo sapiens, GI4503471, Length=444, Percent_Identity=29.0540540540541, Blast_Score=161, Evalue=9e-40,
Organism=Homo sapiens, GI4503475, Length=444, Percent_Identity=28.1531531531532, Blast_Score=160, Evalue=3e-39,
Organism=Homo sapiens, GI194018520, Length=436, Percent_Identity=26.1467889908257, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI194018522, Length=436, Percent_Identity=26.1467889908257, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI194097354, Length=436, Percent_Identity=26.1467889908257, Blast_Score=125, Evalue=5e-29,
Organism=Homo sapiens, GI46094014, Length=431, Percent_Identity=26.2180974477958, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI54607086, Length=321, Percent_Identity=28.9719626168224, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI4503507, Length=283, Percent_Identity=29.3286219081272, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI82546879, Length=432, Percent_Identity=22.9166666666667, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI45593140, Length=416, Percent_Identity=22.3557692307692, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI94966754, Length=135, Percent_Identity=36.2962962962963, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI157426893, Length=282, Percent_Identity=26.9503546099291, Blast_Score=69, Evalue=9e-12,
Organism=Escherichia coli, GI1789737, Length=398, Percent_Identity=73.1155778894472, Blast_Score=592, Evalue=1e-170,
Organism=Escherichia coli, GI1790412, Length=398, Percent_Identity=73.1155778894472, Blast_Score=592, Evalue=1e-170,
Organism=Escherichia coli, GI2367247, Length=337, Percent_Identity=30.8605341246291, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI48994988, Length=280, Percent_Identity=33.2142857142857, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1789108, Length=330, Percent_Identity=26.0606060606061, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17556456, Length=402, Percent_Identity=52.4875621890547, Blast_Score=402, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI25141371, Length=390, Percent_Identity=45.1282051282051, Blast_Score=338, Evalue=3e-93,
Organism=Caenorhabditis elegans, GI17552884, Length=446, Percent_Identity=28.0269058295964, Blast_Score=156, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17569207, Length=446, Percent_Identity=28.0269058295964, Blast_Score=156, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI32566629, Length=437, Percent_Identity=26.5446224256293, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI32566303, Length=443, Percent_Identity=27.5395033860045, Blast_Score=130, Evalue=8e-31,
Organism=Caenorhabditis elegans, GI17506081, Length=278, Percent_Identity=29.136690647482, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI115532067, Length=440, Percent_Identity=26.3636363636364, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI115532065, Length=435, Percent_Identity=25.9770114942529, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI32566301, Length=231, Percent_Identity=30.7359307359307, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17557151, Length=258, Percent_Identity=26.3565891472868, Blast_Score=72, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6324761, Length=399, Percent_Identity=63.1578947368421, Blast_Score=512, Evalue=1e-146,
Organism=Saccharomyces cerevisiae, GI6325337, Length=445, Percent_Identity=29.438202247191, Blast_Score=165, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6319594, Length=445, Percent_Identity=29.438202247191, Blast_Score=165, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6322937, Length=346, Percent_Identity=24.8554913294798, Blast_Score=97, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6320377, Length=345, Percent_Identity=29.2753623188406, Blast_Score=95, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6320863, Length=271, Percent_Identity=28.0442804428044, Blast_Score=86, Evalue=8e-18,
Organism=Saccharomyces cerevisiae, GI6323098, Length=135, Percent_Identity=35.5555555555556, Blast_Score=70, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6323320, Length=286, Percent_Identity=26.2237762237762, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI281363316, Length=396, Percent_Identity=55.8080808080808, Blast_Score=464, Evalue=1e-131,
Organism=Drosophila melanogaster, GI17864358, Length=396, Percent_Identity=55.8080808080808, Blast_Score=464, Evalue=1e-131,
Organism=Drosophila melanogaster, GI19921738, Length=390, Percent_Identity=50.2564102564103, Blast_Score=400, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24652838, Length=456, Percent_Identity=29.3859649122807, Blast_Score=155, Evalue=4e-38,
Organism=Drosophila melanogaster, GI17137572, Length=456, Percent_Identity=29.3859649122807, Blast_Score=155, Evalue=4e-38,
Organism=Drosophila melanogaster, GI45553807, Length=459, Percent_Identity=28.5403050108932, Blast_Score=154, Evalue=1e-37,
Organism=Drosophila melanogaster, GI45553816, Length=459, Percent_Identity=28.5403050108932, Blast_Score=154, Evalue=1e-37,
Organism=Drosophila melanogaster, GI24651721, Length=459, Percent_Identity=28.5403050108932, Blast_Score=154, Evalue=1e-37,
Organism=Drosophila melanogaster, GI17864154, Length=459, Percent_Identity=28.5403050108932, Blast_Score=154, Evalue=1e-37,
Organism=Drosophila melanogaster, GI19922690, Length=308, Percent_Identity=29.5454545454545, Blast_Score=125, Evalue=7e-29,
Organism=Drosophila melanogaster, GI17137380, Length=345, Percent_Identity=29.2753623188406, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI45550900, Length=338, Percent_Identity=28.9940828402367, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24647046, Length=296, Percent_Identity=28.7162162162162, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24647048, Length=296, Percent_Identity=28.7162162162162, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24582462, Length=146, Percent_Identity=34.2465753424658, Blast_Score=65, Evalue=8e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: NA

Molecular weight: Translated: 43594; Mature: 43463

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEKFSRNKPHVNIGTIGHVDHGKTTLTAAISAVLSRRGLAELKDYDNIDNAPEEKERG
CCCCCCCCCCCEEEECEEECCCCCCHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCHHHCC
ITIATSHIEYETDNRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHI
EEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHE
LLSRQVGVPYIVVFMNKADMVDDAELLELVEMEIRELLSSYDFPGDDTPIISGSALKALE
EEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCHHHHHHH
EAKAGQDGEWSAKIMDLMAAVDSYIPTPTRDTEKDFLMPIEDVFSISGRGTVVTGRIEKG
HHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCHHHHHCCCCCCEEEEECCCCC
VVKVGDTIEIVGIKDTQTTTVTGVEMFRKEMDQGEAGDNVGVLLRGTKKEEVIRGMVLAK
EEEECCEEEEEEECCCCCEEHHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHEEC
PKSITPHTDFEAEVYILNKDEGGRHTPFFNNYRPQFYVRTTDVTGSIKLADGVEMVMPGE
CCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCEEEEEEECCCCEEEECCCEEEEECCC
NVRITVSLIAPVALEEGTRFAIREGGKTVGSGVVSKIIK
CEEEEEEEEECEECCCCCEEEECCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
AKEKFSRNKPHVNIGTIGHVDHGKTTLTAAISAVLSRRGLAELKDYDNIDNAPEEKERG
CCCCCCCCCCEEEECEEECCCCCCHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCHHHCC
ITIATSHIEYETDNRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHI
EEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHE
LLSRQVGVPYIVVFMNKADMVDDAELLELVEMEIRELLSSYDFPGDDTPIISGSALKALE
EEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCHHHHHHH
EAKAGQDGEWSAKIMDLMAAVDSYIPTPTRDTEKDFLMPIEDVFSISGRGTVVTGRIEKG
HHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCHHHHHCCCCCCEEEEECCCCC
VVKVGDTIEIVGIKDTQTTTVTGVEMFRKEMDQGEAGDNVGVLLRGTKKEEVIRGMVLAK
EEEECCEEEEEEECCCCCEEHHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHEEC
PKSITPHTDFEAEVYILNKDEGGRHTPFFNNYRPQFYVRTTDVTGSIKLADGVEMVMPGE
CCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCEEEEEEECCCCEEEECCCEEEEECCC
NVRITVSLIAPVALEEGTRFAIREGGKTVGSGVVSKIIK
CEEEEEEEEECEECCCCCEEEECCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA