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The map label for this gene is sdhB [C]

Identifier: 57237494

GI number: 57237494

Start: 445342

End: 446307

Strand: Direct

Name: sdhB [C]

Synonym: CJE0489

Alternate gene names: 57237494

Gene position: 445342-446307 (Clockwise)

Preceding gene: 57237493

Following gene: 57237495

Centisome position: 25.05

GC content: 35.92

Gene sequence:

>966_bases
ATGAAAATCATCATAGATCGTTTTAATGGCAAGGAAAAATATGAACAAAGTTATGATATTGATGATAAGGATATACAAGG
AAAAACTCTGCTCTCTCTTTTACTTTTTATTAAAAAAACAAAAGATATTACTTTAAATTTTACCGCCTCTTGTCAATCTG
CGATTTGTGGGGCTTGTGCAGTTCGTGTGAATGGGCATTCTTATCTTGCTTGTGATACTAAAATGCAAGATTTATTAAAA
GAATATGATAATCCTTCAAGTATACGCATTTCTCCGCTTGGAAATTTTAGAGTAATTTCTGATTTAATTGTGGATTGGGA
GCCTTCTATAGAAAATTTAAGAAAAATTCGTCCTGCCATGGTGGCAAAAAATGAATTTTCAGCGGAAAAGGGCTGTAAAC
AAAGTCAAGAAGAATTTGATCGTATTAGCAAGCAATGGGATTGTATTTTATGTGGTAGCTGTGCTTCAGAGTGTAATAAA
TTAGAAGCAGATAGTAGCGATTATATGCAACCTTTTGTTTTTACTCATGCTTGGCGTGCAGCGGCGGACTCTCGAGGTAA
GGATCCTATGTTGCATGTAAAACCCAGCGTAATGAATGGACTTTGGCTTTGTGTTCATTGTCAAGAGTGCGCTGATCGTT
GTCCAAAGGGTATAAGTTCAGTAAGTGATATAGCAAATTTAAGAGTGATGGCGATTAAAAAAGGTTTAAATGAAGGCTTA
GGGCCTGATCATGCTGAAGCTTTTTATAAAGATTTAGTTGAAGGTTCAGGACGCTTGAATGAAATTTATCTTGCTTTGCG
TTCTGAGGGTGTTATAGGTTCTATGGGTAAAACAGATATAGCTTTTAAACTTATGCGTGCAGGAAAAATGAATCCTATGC
ATGTTTTTGGCGAGGGTGAAATAGAAGGGCATAAAGATTTGGTTAAAATGATAAAGGCGGCACAAGAAGCTGCTGTTAAG
GAGTAA

Upstream 100 bases:

>100_bases
TCTTAAAACATAGCATTGTAAATTTAGAAAATGATGAATTAAAACTTAGTTATAAAGATGTCGTGGTAACTGAGTTTTCA
CTTGATGGAAGGAGAGTACA

Downstream 100 bases:

>100_bases
AATATGCAAAAAGAGTACGCTTTTTTTCCAGGTTGTGTTTTATCTCAAGCTGCAAAAGAATCTAAAATTTCACTTGAAGC
TATTGCACCTATTTTGGGTT

Product: succinate dehydrogenase, iron-sulfur protein subunit

Products: fumarate; reduced acceptor

Alternate protein names: NA

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MKIIIDRFNGKEKYEQSYDIDDKDIQGKTLLSLLLFIKKTKDITLNFTASCQSAICGACAVRVNGHSYLACDTKMQDLLK
EYDNPSSIRISPLGNFRVISDLIVDWEPSIENLRKIRPAMVAKNEFSAEKGCKQSQEEFDRISKQWDCILCGSCASECNK
LEADSSDYMQPFVFTHAWRAAADSRGKDPMLHVKPSVMNGLWLCVHCQECADRCPKGISSVSDIANLRVMAIKKGLNEGL
GPDHAEAFYKDLVEGSGRLNEIYLALRSEGVIGSMGKTDIAFKLMRAGKMNPMHVFGEGEIEGHKDLVKMIKAAQEAAVK
E

Sequences:

>Translated_321_residues
MKIIIDRFNGKEKYEQSYDIDDKDIQGKTLLSLLLFIKKTKDITLNFTASCQSAICGACAVRVNGHSYLACDTKMQDLLK
EYDNPSSIRISPLGNFRVISDLIVDWEPSIENLRKIRPAMVAKNEFSAEKGCKQSQEEFDRISKQWDCILCGSCASECNK
LEADSSDYMQPFVFTHAWRAAADSRGKDPMLHVKPSVMNGLWLCVHCQECADRCPKGISSVSDIANLRVMAIKKGLNEGL
GPDHAEAFYKDLVEGSGRLNEIYLALRSEGVIGSMGKTDIAFKLMRAGKMNPMHVFGEGEIEGHKDLVKMIKAAQEAAVK
E
>Mature_321_residues
MKIIIDRFNGKEKYEQSYDIDDKDIQGKTLLSLLLFIKKTKDITLNFTASCQSAICGACAVRVNGHSYLACDTKMQDLLK
EYDNPSSIRISPLGNFRVISDLIVDWEPSIENLRKIRPAMVAKNEFSAEKGCKQSQEEFDRISKQWDCILCGSCASECNK
LEADSSDYMQPFVFTHAWRAAADSRGKDPMLHVKPSVMNGLWLCVHCQECADRCPKGISSVSDIANLRVMAIKKGLNEGL
GPDHAEAFYKDLVEGSGRLNEIYLALRSEGVIGSMGKTDIAFKLMRAGKMNPMHVFGEGEIEGHKDLVKMIKAAQEAAVK
E

Specific function: Two Distinct, Membrane-Bound, FAD-Containing Enzymes Are Responsible For The Catalysis Of Fumarate And Succinate Interconversion; The Fumarate Reductase Is Used In Anaerobic Growth, And The Succinate Dehydrogenase Is Used In Aerobic Growth. [C]

COG id: COG0479

COG function: function code C; Succinate dehydrogenase/fumarate reductase, Fe-S protein subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Homo sapiens, GI115387094, Length=225, Percent_Identity=29.7777777777778, Blast_Score=100, Evalue=1e-21,
Organism=Escherichia coli, GI1790596, Length=252, Percent_Identity=29.3650793650794, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1786943, Length=226, Percent_Identity=26.5486725663717, Blast_Score=89, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17533915, Length=233, Percent_Identity=30.4721030042918, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6322987, Length=224, Percent_Identity=29.4642857142857, Blast_Score=99, Evalue=7e-22,
Organism=Drosophila melanogaster, GI17137106, Length=235, Percent_Identity=30.6382978723404, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24643156, Length=235, Percent_Identity=28.5106382978723, Blast_Score=99, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006058
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR012675
- InterPro:   IPR004017
- InterPro:   IPR001041
- InterPro:   IPR012285
- InterPro:   IPR009051
- InterPro:   IPR004489 [H]

Pfam domain/function: PF02754 CCG; PF00111 Fer2 [H]

EC number: 1.3.99.1

Molecular weight: Translated: 35759; Mature: 35759

Theoretical pI: Translated: 6.83; Mature: 6.83

Prosite motif: PS00197 2FE2S_FER_1 ; PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
7.8 %Cys+Met (Translated Protein)
4.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
7.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIIIDRFNGKEKYEQSYDIDDKDIQGKTLLSLLLFIKKTKDITLNFTASCQSAICGACA
CEEEEECCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHEE
VRVNGHSYLACDTKMQDLLKEYDNPSSIRISPLGNFRVISDLIVDWEPSIENLRKIRPAM
EEECCCEEEEECHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHH
VAKNEFSAEKGCKQSQEEFDRISKQWDCILCGSCASECNKLEADSSDYMQPFVFTHAWRA
HHCCHHCHHHCHHHHHHHHHHHHHCCCEEEECHHHHHHHHCCCCCCHHHHHHHHHHHHHH
AADSRGKDPMLHVKPSVMNGLWLCVHCQECADRCPKGISSVSDIANLRVMAIKKGLNEGL
HHCCCCCCCEEEECHHHHCCEEEEEEHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCC
GPDHAEAFYKDLVEGSGRLNEIYLALRSEGVIGSMGKTDIAFKLMRAGKMNPMHVFGEGE
CCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCC
IEGHKDLVKMIKAAQEAAVKE
CCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKIIIDRFNGKEKYEQSYDIDDKDIQGKTLLSLLLFIKKTKDITLNFTASCQSAICGACA
CEEEEECCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHEE
VRVNGHSYLACDTKMQDLLKEYDNPSSIRISPLGNFRVISDLIVDWEPSIENLRKIRPAM
EEECCCEEEEECHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHH
VAKNEFSAEKGCKQSQEEFDRISKQWDCILCGSCASECNKLEADSSDYMQPFVFTHAWRA
HHCCHHCHHHCHHHHHHHHHHHHHCCCEEEECHHHHHHHHCCCCCCHHHHHHHHHHHHHH
AADSRGKDPMLHVKPSVMNGLWLCVHCQECADRCPKGISSVSDIANLRVMAIKKGLNEGL
HHCCCCCCCEEEECHHHHCCEEEEEEHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCC
GPDHAEAFYKDLVEGSGRLNEIYLALRSEGVIGSMGKTDIAFKLMRAGKMNPMHVFGEGE
CCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCC
IEGHKDLVKMIKAAQEAAVKE
CCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: ATP; Dithiothreitol; FAD; Fe-S-clusters [C]

Metal ions: Anions; Diphosphate; Fe [C]

Kcat value (1/min): 10000-13000 [C]

Specific activity: 509

Km value (mM): 0.4 {fumarate}} 0.02 {succinate}} 0.3 {ferricyanide}} [C]

Substrates: succinate; acceptor

Specific reaction: succinate + acceptor = fumarate + reduced acceptor

General reaction: Redox reaction [C]

Inhibitor: 5, 5'-Dithiobis (2-nitrobenzoate); Chaotropic reagents [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]