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The map label for this gene is pyk [H]

Identifier: 57237447

GI number: 57237447

Start: 396436

End: 397878

Strand: Reverse

Name: pyk [H]

Synonym: CJE0441

Alternate gene names: 57237447

Gene position: 397878-396436 (Counterclockwise)

Preceding gene: 57237448

Following gene: 57237446

Centisome position: 22.38

GC content: 30.56

Gene sequence:

>1443_bases
ATGCTTAAAAAAACAAAAATTGTAGCCACAGTAGGTCCAGCAAGTGAAAAGGAAGAAATTCTACGCCAAATGATTATCAA
TGGTGTAAATGTTTTCCGTTTAAACTTTTCTCATGGAACACATGAGTATCATAAAAAAAACTTAGATACCATAAGAAGAG
TAGCTAAAGAATTGCATACTCGTATTGGAATTTTACAAGACATTAGTGGTCCTAAAATTCGTACAGGTGAACTTAAAGAA
CCATTTGAATTAAAAAAGGGTGATAGACTTGATTTTTATCGTGAAACAATTTTAGGGGAGAAGATTGCTCAAAATCATTA
TAAAATCAGTATCAATCAAAAATCTATTTTAGATATGTTAAAAATTGATGAATATATTTATCTATATGATGGATCAATTC
GTGCTAAAGTAGAAAACATAGATGATCAAAAAATTGAAACCATTATAGAAAATGATGGTTTTTTAAACTCAAATAAAGGT
ATTAATTTTCCGAATACTAAAATCAATATTGATGTTATTACACAAAAAGATAAAAATGATTTACTTTGGGGTATAAAAAA
TGAAGTAGATTTTTTAGCAATATCTTTCGTACAAAATGCGCACGATATTGATGAAGTTCGTGAAATTTTAGCACAAAATA
ACGCAAAAATATCTATTTTTGCAAAAATTGAAAAATTTGATGCAGTAGAAAATATTGATGAGATTATTAAATCTAGCGAT
GGTATAATGGTTGCAAGAGGAGACTTAGGTATTGAGGTACCTTATTATAAAGTTCCTAATATCCAAAAAGAAATCATACA
AAAAGCAAACAATGCCTCTAAGCCTGTTATTACTGCGACTCAAATGCTTTTTTCATTAGCCAAAAGCAAAACCGCCACAA
GAGCTGAAATTTCAGATGTTGCAAATGCTGTTCTAGATGGAACAGATGCAGTAATGCTAAGTGAAGAAAGTGCCGTTGGT
ACAGATCCTGCAAACGCTGTTGATATAATGTGTCAAACTATAATAGAAACAGAAAAACGCTATCCATATAACAAATTTAA
TGATTTTAATGATTTAGACAATACAGATAAAATCATGCGTTCTTCAGCTCATCTTGCAACAGATCTTAACGCTGATGCTA
TTTTTTCTTTAACAAGTAGCGGAAAATCTGCTATTAAGATTGCGAGATATCGTCCAAATATAGAAATCATAGCAGTAGGA
CATTCTGAAAAAACCTTAAACTCTTTAAGCATAGTTTGGGGAGTTAATCCTGCGATTTTAGTTAACAAAAGTAATGAACT
TACAGAATTATTAAAAGACTCTGTAAGATCAAGTGTTGAAAAAGGTTTTATGGATGAAGATAAATGCTATCTTCTAACTG
CAGGCTTTCCAACAGGGGTAGAAGGCACTAGCAATCTTATCCGCATTCTCAATAAAGAGCAAATTACTTATTATCTACAA
TAA

Upstream 100 bases:

>100_bases
TTACCTAGGAATGGAATTTAACGAAGATAAATTTTCTTCAGAATTATTGTAATCATTTTGTTTCTATTTTTAGCTACAAT
AATTTCAAAAGGAGTTTACC

Downstream 100 bases:

>100_bases
AACATTAAATTCCTTTGAAAATAAGGAATTTAATGTCCTTATAAATTTTTTAAGTCAAAAGTCGATATAAATTAAAGCCT
CACACAAAGGAGCAAATATG

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 480; Mature: 480

Protein sequence:

>480_residues
MLKKTKIVATVGPASEKEEILRQMIINGVNVFRLNFSHGTHEYHKKNLDTIRRVAKELHTRIGILQDISGPKIRTGELKE
PFELKKGDRLDFYRETILGEKIAQNHYKISINQKSILDMLKIDEYIYLYDGSIRAKVENIDDQKIETIIENDGFLNSNKG
INFPNTKINIDVITQKDKNDLLWGIKNEVDFLAISFVQNAHDIDEVREILAQNNAKISIFAKIEKFDAVENIDEIIKSSD
GIMVARGDLGIEVPYYKVPNIQKEIIQKANNASKPVITATQMLFSLAKSKTATRAEISDVANAVLDGTDAVMLSEESAVG
TDPANAVDIMCQTIIETEKRYPYNKFNDFNDLDNTDKIMRSSAHLATDLNADAIFSLTSSGKSAIKIARYRPNIEIIAVG
HSEKTLNSLSIVWGVNPAILVNKSNELTELLKDSVRSSVEKGFMDEDKCYLLTAGFPTGVEGTSNLIRILNKEQITYYLQ

Sequences:

>Translated_480_residues
MLKKTKIVATVGPASEKEEILRQMIINGVNVFRLNFSHGTHEYHKKNLDTIRRVAKELHTRIGILQDISGPKIRTGELKE
PFELKKGDRLDFYRETILGEKIAQNHYKISINQKSILDMLKIDEYIYLYDGSIRAKVENIDDQKIETIIENDGFLNSNKG
INFPNTKINIDVITQKDKNDLLWGIKNEVDFLAISFVQNAHDIDEVREILAQNNAKISIFAKIEKFDAVENIDEIIKSSD
GIMVARGDLGIEVPYYKVPNIQKEIIQKANNASKPVITATQMLFSLAKSKTATRAEISDVANAVLDGTDAVMLSEESAVG
TDPANAVDIMCQTIIETEKRYPYNKFNDFNDLDNTDKIMRSSAHLATDLNADAIFSLTSSGKSAIKIARYRPNIEIIAVG
HSEKTLNSLSIVWGVNPAILVNKSNELTELLKDSVRSSVEKGFMDEDKCYLLTAGFPTGVEGTSNLIRILNKEQITYYLQ
>Mature_480_residues
MLKKTKIVATVGPASEKEEILRQMIINGVNVFRLNFSHGTHEYHKKNLDTIRRVAKELHTRIGILQDISGPKIRTGELKE
PFELKKGDRLDFYRETILGEKIAQNHYKISINQKSILDMLKIDEYIYLYDGSIRAKVENIDDQKIETIIENDGFLNSNKG
INFPNTKINIDVITQKDKNDLLWGIKNEVDFLAISFVQNAHDIDEVREILAQNNAKISIFAKIEKFDAVENIDEIIKSSD
GIMVARGDLGIEVPYYKVPNIQKEIIQKANNASKPVITATQMLFSLAKSKTATRAEISDVANAVLDGTDAVMLSEESAVG
TDPANAVDIMCQTIIETEKRYPYNKFNDFNDLDNTDKIMRSSAHLATDLNADAIFSLTSSGKSAIKIARYRPNIEIIAVG
HSEKTLNSLSIVWGVNPAILVNKSNELTELLKDSVRSSVEKGFMDEDKCYLLTAGFPTGVEGTSNLIRILNKEQITYYLQ

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PEP- utilizing enzyme family [H]

Homologues:

Organism=Homo sapiens, GI33286418, Length=491, Percent_Identity=36.6598778004073, Blast_Score=295, Evalue=8e-80,
Organism=Homo sapiens, GI33286422, Length=498, Percent_Identity=36.9477911646586, Blast_Score=293, Evalue=3e-79,
Organism=Homo sapiens, GI33286420, Length=498, Percent_Identity=36.9477911646586, Blast_Score=293, Evalue=3e-79,
Organism=Homo sapiens, GI10835121, Length=492, Percent_Identity=34.7560975609756, Blast_Score=282, Evalue=5e-76,
Organism=Homo sapiens, GI32967597, Length=492, Percent_Identity=34.7560975609756, Blast_Score=282, Evalue=6e-76,
Organism=Homo sapiens, GI310128732, Length=289, Percent_Identity=39.7923875432526, Blast_Score=207, Evalue=2e-53,
Organism=Homo sapiens, GI310128730, Length=289, Percent_Identity=39.7923875432526, Blast_Score=207, Evalue=2e-53,
Organism=Homo sapiens, GI310128736, Length=237, Percent_Identity=35.4430379746835, Blast_Score=152, Evalue=6e-37,
Organism=Homo sapiens, GI310128734, Length=237, Percent_Identity=35.4430379746835, Blast_Score=152, Evalue=6e-37,
Organism=Homo sapiens, GI310128738, Length=216, Percent_Identity=34.7222222222222, Blast_Score=131, Evalue=1e-30,
Organism=Escherichia coli, GI1787965, Length=475, Percent_Identity=37.6842105263158, Blast_Score=288, Evalue=4e-79,
Organism=Escherichia coli, GI1788160, Length=480, Percent_Identity=35.4166666666667, Blast_Score=263, Evalue=2e-71,
Organism=Caenorhabditis elegans, GI17544584, Length=486, Percent_Identity=37.6543209876543, Blast_Score=293, Evalue=2e-79,
Organism=Caenorhabditis elegans, GI71984413, Length=491, Percent_Identity=36.2525458248472, Blast_Score=280, Evalue=9e-76,
Organism=Caenorhabditis elegans, GI71984406, Length=491, Percent_Identity=36.2525458248472, Blast_Score=280, Evalue=9e-76,
Organism=Caenorhabditis elegans, GI17506829, Length=491, Percent_Identity=36.2525458248472, Blast_Score=280, Evalue=9e-76,
Organism=Caenorhabditis elegans, GI17506831, Length=491, Percent_Identity=36.2525458248472, Blast_Score=280, Evalue=1e-75,
Organism=Saccharomyces cerevisiae, GI6319279, Length=486, Percent_Identity=34.156378600823, Blast_Score=271, Evalue=2e-73,
Organism=Saccharomyces cerevisiae, GI6324923, Length=485, Percent_Identity=33.1958762886598, Blast_Score=247, Evalue=2e-66,
Organism=Drosophila melanogaster, GI24648964, Length=494, Percent_Identity=37.4493927125506, Blast_Score=310, Evalue=1e-84,
Organism=Drosophila melanogaster, GI28571814, Length=494, Percent_Identity=37.4493927125506, Blast_Score=310, Evalue=1e-84,
Organism=Drosophila melanogaster, GI24648966, Length=410, Percent_Identity=34.390243902439, Blast_Score=238, Evalue=5e-63,
Organism=Drosophila melanogaster, GI24581235, Length=484, Percent_Identity=29.5454545454545, Blast_Score=229, Evalue=4e-60,
Organism=Drosophila melanogaster, GI24646914, Length=338, Percent_Identity=32.2485207100592, Blast_Score=152, Evalue=6e-37,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 53907; Mature: 53907

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: PS00659 GLYCOSYL_HYDROL_F5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKKTKIVATVGPASEKEEILRQMIINGVNVFRLNFSHGTHEYHKKNLDTIRRVAKELHT
CCCCEEEEEEECCCCHHHHHHHHHHHCCCEEEEEECCCCCHHHHHCCHHHHHHHHHHHHH
RIGILQDISGPKIRTGELKEPFELKKGDRLDFYRETILGEKIAQNHYKISINQKSILDML
HHHHHHCCCCCCEECCCCCCCHHCCCCCCHHHHHHHHHHHHHHCCCEEEEECHHHHHHHH
KIDEYIYLYDGSIRAKVENIDDQKIETIIENDGFLNSNKGINFPNTKINIDVITQKDKND
HHCCEEEEECCCEEEEECCCCHHHHHHHHHCCCEECCCCCCCCCCCEEEEEEEEECCCCC
LLWGIKNEVDFLAISFVQNAHDIDEVREILAQNNAKISIFAKIEKFDAVENIDEIIKSSD
CEEECCCCCCEEEEEHHHCCCCHHHHHHHHHCCCCEEEEEEEEHHHHHHHHHHHHHHCCC
GIMVARGDLGIEVPYYKVPNIQKEIIQKANNASKPVITATQMLFSLAKSKTATRAEISDV
CEEEEECCCCEECCCEECCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHH
ANAVLDGTDAVMLSEESAVGTDPANAVDIMCQTIIETEKRYPYNKFNDFNDLDNTDKIMR
HHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHH
SSAHLATDLNADAIFSLTSSGKSAIKIARYRPNIEIIAVGHSEKTLNSLSIVWGVNPAIL
HCCHHHCCCCCCCEEEECCCCCCEEEEEEECCCEEEEEECCCCCCHHCEEEEECCCCEEE
VNKSNELTELLKDSVRSSVEKGFMDEDKCYLLTAGFPTGVEGTSNLIRILNKEQITYYLQ
EECCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCHHHHHHHHCCCCEEEEEC
>Mature Secondary Structure
MLKKTKIVATVGPASEKEEILRQMIINGVNVFRLNFSHGTHEYHKKNLDTIRRVAKELHT
CCCCEEEEEEECCCCHHHHHHHHHHHCCCEEEEEECCCCCHHHHHCCHHHHHHHHHHHHH
RIGILQDISGPKIRTGELKEPFELKKGDRLDFYRETILGEKIAQNHYKISINQKSILDML
HHHHHHCCCCCCEECCCCCCCHHCCCCCCHHHHHHHHHHHHHHCCCEEEEECHHHHHHHH
KIDEYIYLYDGSIRAKVENIDDQKIETIIENDGFLNSNKGINFPNTKINIDVITQKDKND
HHCCEEEEECCCEEEEECCCCHHHHHHHHHCCCEECCCCCCCCCCCEEEEEEEEECCCCC
LLWGIKNEVDFLAISFVQNAHDIDEVREILAQNNAKISIFAKIEKFDAVENIDEIIKSSD
CEEECCCCCCEEEEEHHHCCCCHHHHHHHHHCCCCEEEEEEEEHHHHHHHHHHHHHHCCC
GIMVARGDLGIEVPYYKVPNIQKEIIQKANNASKPVITATQMLFSLAKSKTATRAEISDV
CEEEEECCCCEECCCEECCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHH
ANAVLDGTDAVMLSEESAVGTDPANAVDIMCQTIIETEKRYPYNKFNDFNDLDNTDKIMR
HHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHH
SSAHLATDLNADAIFSLTSSGKSAIKIARYRPNIEIIAVGHSEKTLNSLSIVWGVNPAIL
HCCHHHCCCCCCCEEEECCCCCCEEEEEEECCCEEEEEECCCCCCHHCEEEEECCCCEEE
VNKSNELTELLKDSVRSSVEKGFMDEDKCYLLTAGFPTGVEGTSNLIRILNKEQITYYLQ
EECCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCHHHHHHHHCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA