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The map label for this gene is ribH

Identifier: 57237438

GI number: 57237438

Start: 386825

End: 387289

Strand: Reverse

Name: ribH

Synonym: CJE0432

Alternate gene names: 57237438

Gene position: 387289-386825 (Counterclockwise)

Preceding gene: 57237439

Following gene: 57237437

Centisome position: 21.78

GC content: 35.7

Gene sequence:

>465_bases
ATGAATATTATTGAAGGAAAATTAAATTTAGACTCAAATACAAAAATTGCTATCATTAATGCAAGATTTAATCATATCAT
CACAGATCGCCTTGTAGAAGGAGCAAAAGATGCTTTTTTAAGACACGGGGGCAAAGAGGAAAATCTAAGCCTTATACTTG
TTCCTGGTGCTTTTGAACTTCCGTATGCGTTAAAAAAAGCTATAGAAAGCAAAAAATTTGATGCAATTTGTTGCGTAGGC
GCAGTAATTCGTGGCTCAACTCCGCATTTTGACTATGTTTCAGCAGAAACCACCAAAGGCATAGCCAATGTGAGTTTAAA
TCATAATATCCCTGTAAGCTTTGGTGTTTTAACTACAGACACAATAGAACAAGCCATAGAAAGAGCTGGAAGCAAAGCAG
GCAATAAAGGCTTTGAAGCTATGACAACTGTAATCGAGATGTTAAATTTAAGCAAGGAGCTTTAA

Upstream 100 bases:

>100_bases
GCGAGGCTTTATGCGATGGACCTAATATGCTTAATCTTACACGCCTTAAAAATTGCGTTAATACATTACTAGAAATACAA
AATATCATAAAGGAAAACAA

Downstream 100 bases:

>100_bases
TGGCAACGCGTCATCAAGTTCGCCAAAGCGTCATCTCCTTACTTTATGCTTTTGAATTAAATTCTCAAAACAACGTTTTT
GTAGATGAAATTTTAGATGA

Product: 6,7-dimethyl-8-ribityllumazine synthase

Products: NA

Alternate protein names: DMRL synthase; Lumazine synthase; Riboflavin synthase beta chain

Number of amino acids: Translated: 154; Mature: 154

Protein sequence:

>154_residues
MNIIEGKLNLDSNTKIAIINARFNHIITDRLVEGAKDAFLRHGGKEENLSLILVPGAFELPYALKKAIESKKFDAICCVG
AVIRGSTPHFDYVSAETTKGIANVSLNHNIPVSFGVLTTDTIEQAIERAGSKAGNKGFEAMTTVIEMLNLSKEL

Sequences:

>Translated_154_residues
MNIIEGKLNLDSNTKIAIINARFNHIITDRLVEGAKDAFLRHGGKEENLSLILVPGAFELPYALKKAIESKKFDAICCVG
AVIRGSTPHFDYVSAETTKGIANVSLNHNIPVSFGVLTTDTIEQAIERAGSKAGNKGFEAMTTVIEMLNLSKEL
>Mature_154_residues
MNIIEGKLNLDSNTKIAIINARFNHIITDRLVEGAKDAFLRHGGKEENLSLILVPGAFELPYALKKAIESKKFDAICCVG
AVIRGSTPHFDYVSAETTKGIANVSLNHNIPVSFGVLTTDTIEQAIERAGSKAGNKGFEAMTTVIEMLNLSKEL

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The beta subunit catalyzes

COG id: COG0054

COG function: function code H; Riboflavin synthase beta-chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DMRL synthase family

Homologues:

Organism=Escherichia coli, GI1786617, Length=155, Percent_Identity=50.9677419354839, Blast_Score=157, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6324429, Length=124, Percent_Identity=39.5161290322581, Blast_Score=98, Evalue=5e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RISB_CAMJ8 (A8FKH0)

Other databases:

- EMBL:   CP000814
- RefSeq:   YP_001481934.1
- ProteinModelPortal:   A8FKH0
- SMR:   A8FKH0
- STRING:   A8FKH0
- GeneID:   5618089
- GenomeReviews:   CP000814_GR
- KEGG:   cju:C8J_0358
- eggNOG:   COG0054
- HOGENOM:   HBG311126
- OMA:   KAGNKGW
- ProtClustDB:   PRK00061
- BioCyc:   CJEJ407148:C8J_0358-MONOMER
- HAMAP:   MF_00178
- InterPro:   IPR002180
- Gene3D:   G3DSA:3.40.50.960
- PANTHER:   PTHR21058
- TIGRFAMs:   TIGR00114

Pfam domain/function: PF00885 DMRL_synthase; SSF52121 DMRL_synthase

EC number: =2.5.1.9

Molecular weight: Translated: 16686; Mature: 16686

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIIEGKLNLDSNTKIAIINARFNHIITDRLVEGAKDAFLRHGGKEENLSLILVPGAFEL
CCEECCEEECCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCC
PYALKKAIESKKFDAICCVGAVIRGSTPHFDYVSAETTKGIANVSLNHNIPVSFGVLTTD
CHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCEECCCCCCCEEEEEECCCCCEEECEEEHH
TIEQAIERAGSKAGNKGFEAMTTVIEMLNLSKEL
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNIIEGKLNLDSNTKIAIINARFNHIITDRLVEGAKDAFLRHGGKEENLSLILVPGAFEL
CCEECCEEECCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCC
PYALKKAIESKKFDAICCVGAVIRGSTPHFDYVSAETTKGIANVSLNHNIPVSFGVLTTD
CHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCEECCCCCCCEEEEEECCCCCEEECEEEHH
TIEQAIERAGSKAGNKGFEAMTTVIEMLNLSKEL
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA