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The map label for this gene is pyrF [H]

Identifier: 57237436

GI number: 57237436

Start: 385591

End: 386430

Strand: Reverse

Name: pyrF [H]

Synonym: CJE0430

Alternate gene names: 57237436

Gene position: 386430-385591 (Counterclockwise)

Preceding gene: 57237437

Following gene: 57237435

Centisome position: 21.74

GC content: 30.36

Gene sequence:

>840_bases
ATGAAGCTTTGTGTCGCACTTGATCTTTCTACAAAAGAAGAATGCTTACAACTTGCCAAAGAATTAAAAAATTTAGACAT
ATGGTTAAAAGTAGGTTTAAGAGCATATTTAAGAGATGGTTTTAAATTCATAGAAGAACTTAAAAAAGTTGATGATTTTA
AAATATTTCTTGATCTTAAAATTCATGATATTCCCAACACTATGGCAGATGCTTGCGAAGAAGTATCAAAACTTGGTGTT
GATATGATCAATATCCACGCAAGTGCTGGAAAAATAGCCATACAAGAAGTTATGACACGGCTTAGCAAATTTTCAAAAAG
ACCTTTAGTTTTAGCAGTTTCTGCTCTTACGAGTTTTGATGAAGAAAATTTCTTTAGTATTTATAGACAAAAAATTGAAG
AAGCTGTAATTAATTTTTCAAAAATATCCTATGAAAATGGACTTGATGGTATGGTATGCTCTGTTTTTGAAAGCAAAAAA
ATTAAAGAGCACACAAGTTCAAATTTCTTAACCCTTACTCCTGGAATTCGTCCTTTTGGAGAAACAAGTGATGATCAAAA
ACGTGTTGCTAATCTTGCAATGGCTAGAGAAAATTTGAGTGATTATATCGTTGTGGGAAGGCCTATATATAAAAATGAAA
ATCCTAGGGCTGTATGTGAAAAAATTTTAAACAAAATACACAGAAAAAATATAAGCGAAAACGATATAGAGCAAAATTAT
GAAGTAATTCAGCAAAAAGAATGGGATATGTGTAATCACTTTGAAGAATGGATTAAAACTCAGCCTGATAAAGAACATGC
TTTAAAAGAATTTTATGCTAAGTGTGGGATAAAATATTGA

Upstream 100 bases:

>100_bases
TTGTCATCAATGAGGCTATAGAACTTGCTAAAGAACTTGCTAATGACAATTCTCCAAAATTTATAAATGGAGTTTTAGAT
GCCTTAATAAAGGCTAAAAA

Downstream 100 bases:

>100_bases
ATTATCAACAATTAGAATGCGATTACTTTAATCTTTACAATCAGTTTCTTAGCGTGGATTTTCAAATTTCATTATTTGAA
AAAGATCATAAAGGTTTAAT

Product: orotidine 5'-phosphate decarboxylase

Products: NA

Alternate protein names: OMP decarboxylase; OMPDCase; OMPdecase [H]

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MKLCVALDLSTKEECLQLAKELKNLDIWLKVGLRAYLRDGFKFIEELKKVDDFKIFLDLKIHDIPNTMADACEEVSKLGV
DMINIHASAGKIAIQEVMTRLSKFSKRPLVLAVSALTSFDEENFFSIYRQKIEEAVINFSKISYENGLDGMVCSVFESKK
IKEHTSSNFLTLTPGIRPFGETSDDQKRVANLAMARENLSDYIVVGRPIYKNENPRAVCEKILNKIHRKNISENDIEQNY
EVIQQKEWDMCNHFEEWIKTQPDKEHALKEFYAKCGIKY

Sequences:

>Translated_279_residues
MKLCVALDLSTKEECLQLAKELKNLDIWLKVGLRAYLRDGFKFIEELKKVDDFKIFLDLKIHDIPNTMADACEEVSKLGV
DMINIHASAGKIAIQEVMTRLSKFSKRPLVLAVSALTSFDEENFFSIYRQKIEEAVINFSKISYENGLDGMVCSVFESKK
IKEHTSSNFLTLTPGIRPFGETSDDQKRVANLAMARENLSDYIVVGRPIYKNENPRAVCEKILNKIHRKNISENDIEQNY
EVIQQKEWDMCNHFEEWIKTQPDKEHALKEFYAKCGIKY
>Mature_279_residues
MKLCVALDLSTKEECLQLAKELKNLDIWLKVGLRAYLRDGFKFIEELKKVDDFKIFLDLKIHDIPNTMADACEEVSKLGV
DMINIHASAGKIAIQEVMTRLSKFSKRPLVLAVSALTSFDEENFFSIYRQKIEEAVINFSKISYENGLDGMVCSVFESKK
IKEHTSSNFLTLTPGIRPFGETSDDQKRVANLAMARENLSDYIVVGRPIYKNENPRAVCEKILNKIHRKNISENDIEQNY
EVIQQKEWDMCNHFEEWIKTQPDKEHALKEFYAKCGIKY

Specific function: Catalyzes the decarboxylation of orotidine 5'- monophosphate (OMP) to uridine 5'-monophosphate (UMP) [H]

COG id: COG0284

COG function: function code F; Orotidine-5'-phosphate decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the OMP decarboxylase family. Type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787537, Length=229, Percent_Identity=34.061135371179, Blast_Score=130, Evalue=1e-31,

Paralogues:

None

Copy number: 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR014732
- InterPro:   IPR018089
- InterPro:   IPR001754
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00215 OMPdecase [H]

EC number: =4.1.1.23 [H]

Molecular weight: Translated: 32243; Mature: 32243

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00156 OMPDECASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLCVALDLSTKEECLQLAKELKNLDIWLKVGLRAYLRDGFKFIEELKKVDDFKIFLDLK
CCEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEE
IHDIPNTMADACEEVSKLGVDMINIHASAGKIAIQEVMTRLSKFSKRPLVLAVSALTSFD
EECCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCEEHHHHHHHCC
EENFFSIYRQKIEEAVINFSKISYENGLDGMVCSVFESKKIKEHTSSNFLTLTPGIRPFG
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCC
ETSDDQKRVANLAMARENLSDYIVVGRPIYKNENPRAVCEKILNKIHRKNISENDIEQNY
CCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHH
EVIQQKEWDMCNHFEEWIKTQPDKEHALKEFYAKCGIKY
HHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKLCVALDLSTKEECLQLAKELKNLDIWLKVGLRAYLRDGFKFIEELKKVDDFKIFLDLK
CCEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEE
IHDIPNTMADACEEVSKLGVDMINIHASAGKIAIQEVMTRLSKFSKRPLVLAVSALTSFD
EECCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCEEHHHHHHHCC
EENFFSIYRQKIEEAVINFSKISYENGLDGMVCSVFESKKIKEHTSSNFLTLTPGIRPFG
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCC
ETSDDQKRVANLAMARENLSDYIVVGRPIYKNENPRAVCEKILNKIHRKNISENDIEQNY
CCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHH
EVIQQKEWDMCNHFEEWIKTQPDKEHALKEFYAKCGIKY
HHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10688204 [H]