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The map label for this gene is serA [C]

Identifier: 57237428

GI number: 57237428

Start: 378875

End: 379810

Strand: Direct

Name: serA [C]

Synonym: CJE0422

Alternate gene names: 57237428

Gene position: 378875-379810 (Clockwise)

Preceding gene: 57237427

Following gene: 57237429

Centisome position: 21.31

GC content: 30.24

Gene sequence:

>936_bases
ATGAAAATTGTTTGTTTAGATGCGGCAACTTTAGGGGATTATGATTTAAGTGTTTTTGAAAAATTTGGTTCTTTGCAAAT
TTATACCACAACAAATAAAGAGCAAACCATAGAGCGTTTAAAAGATGCTAATGTCGCCATGACAAACAAGGTTGTAATTG
ATAAAGATGTGATTGATGCTTGTAAAAATTTAAAACTCATTTTGGAAACTGCTACAGGGGTTAATAATATCGATATTGAG
TATGCTAAAGAAAAAGGTATTATTGTAAAAAATGCTGCTGGGTATTCTACTATGAGTGTGGTGCAACATACTTTTGCATT
TATGTTTGCTTTTTTAAATCAAGTGCCTTATTATGACAAATGGAGCAAAGAGGGTAAATGGTGTGAAAGTCCTATTTTTA
CAGATTATAGTAGAATTTTAAACACCTTAAGTGGTAAAAAACATGGTATTATAGGTTTAGGAACCATAGGTAAAGAAGTG
GCAAAAATTTCAAAGGCTTTTGGAGCCGAAATTTATTATTATTCTACGAGTGGAGCTAATAAAAACGCAGATTTTGTACA
TTTGGAATTAAAAGATTTATTAAAAACTTGCGATATTATAAGTATCCACGCACCTTTAAATGAAAAAACCAAAAATTTAC
TCGCCTTTGAAGAATTAAAGCTTTTAAAAGATAATGCGATTTTGATTAATGTAGGAAGAGGTGGGATTGTCAATGAAAAT
GATTTAGCTAAAATCATAGATGAAAAAAATATTCGTGTAGGGCTTGATGTTTTAGAAATTGAACCTATGATGAAAAATCA
TCCACTTTTAAGCATTAAAAATAAAGAAAATCTTATTATCACTCCTCATGTTGCGTGGGCGAGCAAGGAGGCTTTAAATG
CTTTAATGGATATAGTTTATAATAATTTAAAAGAATGGATAGAAAATGGCAAGTGA

Upstream 100 bases:

>100_bases
TTTTCTTTGCTTATGAAGGGTGTGGCTTAGGATTTAGAAAAGGAGGACTTGTGCTTGATAATTATTCTAAATTTGTAGGA
CATATTATAAAGGATTAGAA

Downstream 100 bases:

>100_bases
ACATAGTTTTGATATTTCAGCAGCTTTAGATAAACAAGAGTTAAAAAATGCTTTTGAACAAGCTAAAAAAGAACTTGATA
GTCGTTATGACTTAAAAGGA

Product: 2-hydroxyacid dehydrogenase

Products: 3-Phosphohydroxypyruvate; H+; Nicotinamide adenine dinucleotide - reduced [C]

Alternate protein names: NA

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MKIVCLDAATLGDYDLSVFEKFGSLQIYTTTNKEQTIERLKDANVAMTNKVVIDKDVIDACKNLKLILETATGVNNIDIE
YAKEKGIIVKNAAGYSTMSVVQHTFAFMFAFLNQVPYYDKWSKEGKWCESPIFTDYSRILNTLSGKKHGIIGLGTIGKEV
AKISKAFGAEIYYYSTSGANKNADFVHLELKDLLKTCDIISIHAPLNEKTKNLLAFEELKLLKDNAILINVGRGGIVNEN
DLAKIIDEKNIRVGLDVLEIEPMMKNHPLLSIKNKENLIITPHVAWASKEALNALMDIVYNNLKEWIENGK

Sequences:

>Translated_311_residues
MKIVCLDAATLGDYDLSVFEKFGSLQIYTTTNKEQTIERLKDANVAMTNKVVIDKDVIDACKNLKLILETATGVNNIDIE
YAKEKGIIVKNAAGYSTMSVVQHTFAFMFAFLNQVPYYDKWSKEGKWCESPIFTDYSRILNTLSGKKHGIIGLGTIGKEV
AKISKAFGAEIYYYSTSGANKNADFVHLELKDLLKTCDIISIHAPLNEKTKNLLAFEELKLLKDNAILINVGRGGIVNEN
DLAKIIDEKNIRVGLDVLEIEPMMKNHPLLSIKNKENLIITPHVAWASKEALNALMDIVYNNLKEWIENGK
>Mature_311_residues
MKIVCLDAATLGDYDLSVFEKFGSLQIYTTTNKEQTIERLKDANVAMTNKVVIDKDVIDACKNLKLILETATGVNNIDIE
YAKEKGIIVKNAAGYSTMSVVQHTFAFMFAFLNQVPYYDKWSKEGKWCESPIFTDYSRILNTLSGKKHGIIGLGTIGKEV
AKISKAFGAEIYYYSTSGANKNADFVHLELKDLLKTCDIISIHAPLNEKTKNLLAFEELKLLKDNAILINVGRGGIVNEN
DLAKIIDEKNIRVGLDVLEIEPMMKNHPLLSIKNKENLIITPHVAWASKEALNALMDIVYNNLKEWIENGK

Specific function: Serine biosynthesis; first step. [C]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308577, Length=277, Percent_Identity=31.4079422382672, Blast_Score=129, Evalue=2e-30,
Organism=Homo sapiens, GI4557497, Length=256, Percent_Identity=31.25, Blast_Score=119, Evalue=3e-27,
Organism=Homo sapiens, GI61743967, Length=256, Percent_Identity=31.25, Blast_Score=119, Evalue=3e-27,
Organism=Homo sapiens, GI145580578, Length=256, Percent_Identity=30.46875, Blast_Score=111, Evalue=7e-25,
Organism=Homo sapiens, GI4557499, Length=256, Percent_Identity=30.46875, Blast_Score=111, Evalue=7e-25,
Organism=Homo sapiens, GI145580575, Length=256, Percent_Identity=30.46875, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI6912396, Length=261, Percent_Identity=29.1187739463602, Blast_Score=109, Evalue=3e-24,
Organism=Escherichia coli, GI1789279, Length=286, Percent_Identity=30.0699300699301, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI87082289, Length=261, Percent_Identity=25.6704980842912, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI1787645, Length=253, Percent_Identity=23.7154150197628, Blast_Score=84, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17532191, Length=253, Percent_Identity=30.0395256916996, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI25147481, Length=272, Percent_Identity=27.2058823529412, Blast_Score=92, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6324055, Length=336, Percent_Identity=27.3809523809524, Blast_Score=105, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6320925, Length=331, Percent_Identity=26.8882175226586, Blast_Score=103, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6322116, Length=297, Percent_Identity=26.2626262626263, Blast_Score=94, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6324964, Length=311, Percent_Identity=25.4019292604502, Blast_Score=87, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24646446, Length=267, Percent_Identity=29.9625468164794, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24646448, Length=267, Percent_Identity=29.9625468164794, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24646452, Length=267, Percent_Identity=29.9625468164794, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24646450, Length=267, Percent_Identity=29.9625468164794, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI62472511, Length=267, Percent_Identity=29.9625468164794, Blast_Score=118, Evalue=6e-27,
Organism=Drosophila melanogaster, GI28574286, Length=274, Percent_Identity=26.2773722627737, Blast_Score=101, Evalue=6e-22,
Organism=Drosophila melanogaster, GI19921140, Length=255, Percent_Identity=27.0588235294118, Blast_Score=97, Evalue=9e-21,
Organism=Drosophila melanogaster, GI28571528, Length=304, Percent_Identity=29.2763157894737, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24585516, Length=266, Percent_Identity=24.812030075188, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI45552429, Length=291, Percent_Identity=23.7113402061856, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI28574284, Length=291, Percent_Identity=23.7113402061856, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI45551003, Length=291, Percent_Identity=23.7113402061856, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24585514, Length=291, Percent_Identity=23.7113402061856, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI28574282, Length=291, Percent_Identity=23.7113402061856, Blast_Score=73, Evalue=3e-13,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: 1.1.1.95 [C]

Molecular weight: Translated: 34823; Mature: 34823

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIVCLDAATLGDYDLSVFEKFGSLQIYTTTNKEQTIERLKDANVAMTNKVVIDKDVIDA
CEEEEEECCCCCCCHHHHHHHHCCEEEEEECCHHHHHHHHHCCCEEEECEEEECHHHHHH
CKNLKLILETATGVNNIDIEYAKEKGIIVKNAAGYSTMSVVQHTFAFMFAFLNQVPYYDK
HHCCEEEEEECCCCCCCEEEEECCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
WSKEGKWCESPIFTDYSRILNTLSGKKHGIIGLGTIGKEVAKISKAFGAEIYYYSTSGAN
CCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHCCEEEEEECCCCC
KNADFVHLELKDLLKTCDIISIHAPLNEKTKNLLAFEELKLLKDNAILINVGRGGIVNEN
CCCCEEEEEHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCH
DLAKIIDEKNIRVGLDVLEIEPMMKNHPLLSIKNKENLIITPHVAWASKEALNALMDIVY
HHHHHHCCCCCEECCEEEEECHHHHCCCEEEEECCCCEEEECCHHHHHHHHHHHHHHHHH
NNLKEWIENGK
HHHHHHHHCCC
>Mature Secondary Structure
MKIVCLDAATLGDYDLSVFEKFGSLQIYTTTNKEQTIERLKDANVAMTNKVVIDKDVIDA
CEEEEEECCCCCCCHHHHHHHHCCEEEEEECCHHHHHHHHHCCCEEEECEEEECHHHHHH
CKNLKLILETATGVNNIDIEYAKEKGIIVKNAAGYSTMSVVQHTFAFMFAFLNQVPYYDK
HHCCEEEEEECCCCCCCEEEEECCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
WSKEGKWCESPIFTDYSRILNTLSGKKHGIIGLGTIGKEVAKISKAFGAEIYYYSTSGAN
CCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHCCEEEEEECCCCC
KNADFVHLELKDLLKTCDIISIHAPLNEKTKNLLAFEELKLLKDNAILINVGRGGIVNEN
CCCCEEEEEHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCH
DLAKIIDEKNIRVGLDVLEIEPMMKNHPLLSIKNKENLIITPHVAWASKEALNALMDIVY
HHHHHHCCCCCEECCEEEEECHHHHCCCEEEEECCCCEEEECCHHHHHHHHHHHHHHHHH
NNLKEWIENGK
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-Phospho-D-glycerate; Nicotinamide adenine dinucleotide [C]

Specific reaction: 3-Phospho-D-glycerate + Nicotinamide adenine dinucleotide --> 3-Phosphohydroxypyruvate + H+ + Nicotinamide adenine dinucleotide - reduced [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]