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The map label for this gene is cmeA [H]

Identifier: 57237422

GI number: 57237422

Start: 373404

End: 374507

Strand: Reverse

Name: cmeA [H]

Synonym: CJE0416

Alternate gene names: 57237422

Gene position: 374507-373404 (Counterclockwise)

Preceding gene: 57237423

Following gene: 57237421

Centisome position: 21.07

GC content: 31.52

Gene sequence:

>1104_bases
ATGAAATTATTTCAAAAAAATACTATTTTAGCTTTAGGTGTTGTGCTTTTACTCACTGCTTGCAGCAAAGAAGAAGCACC
AAAAATACAAATGCCGCCTCAACCTGTAACAACCATGAGTGCTAAATCTGAAGATTTACCACTTAGTTTTACTTACCCTG
CTAAACTTGTCAGTGATTATGATGTCATTATAAAACCTCAAGTTAGTGGCGTAATAGAAAATAAACTTTTTAAAGCTGGA
GATAAAGTAAAAAAAGGACAAACATTATTTATTATAGAACAAGATAAATTTAAAGCTAGTGTTGATTCGGCTTACGGACA
AGCTTTGATGGCTAAAGCAACTTTCGAAAATGCAAGCAAGGATTTTAATCGTTCTAAAGCTCTTTTTAGTAAAAGTGCAA
TCTCTCAAAAGGAATACGACTCTTCTCTTGCTACATTTAACAATTCAAAAGCTAGTCTAGCAAGTGCTAGAGCACAGCTT
GCAAATGCAAGAATTGATCTAGATCATACCGAAATAAAAGCTCCTTTTGATGGTACTATAGGAGATGCTTTAGTTAATAT
AGGAGATTATGTAAGTGCTTCAACAACTGAACTAGTTAGAGTTACAAATTTAAATCCTATTTACGCAGATTTCTTTATTT
CAGATACAGATAAACTAAATTTAGTCCGCAATACTCAAAGTGGAAAATGGGATTTAGACAGCATTCATGCAAATTTAAAT
CTTAATGGAGAAACCGTTCAAGGCAAACTTTATTTTATTGATTCTGTTATAGATGCTAATAGTGGAACAGTAAAAGCCAA
AGCTATATTTGACAACAACAACTCAACACTTTTACCAGGTGCTTTTGCAACAATTACTTCAGAAGGTTTTATACAAAAAA
ATGGCTTTAAAGTGCCTCAAATAGCTGTTAAACAAAATCAAAATGATGTTTATGTTCTTCTTGTTAAAAATGGAAAAGTA
GAAAAATCTTCTGTACATATAAGCTACCAAAACAATGAATATGCCATTATTGACAAAGGATTGCAAAATGGCGATAAAAT
CATTTTAGATAACTTTAAAAAAATTCAAGTTGGTAGCGAAGTTAAAGAAATTGGAGCACAATAA

Upstream 100 bases:

>100_bases
AGCTTAAAAATAATTTTATTAACCAAAATTAAGATATAATTAGCCAAAAATTTTTGTAATAAATATTACAATTTTTAATT
TAATTTTTCAAGGCAAAACC

Downstream 100 bases:

>100_bases
TGTTTTCTAAATTTTTTATCGAAAGACCTGTTTTTGCTTCAGTTGTTGCAATTATCATTTCTTTAGCTGGAGCTATAGGT
CTTACAAATTTACCTATAGA

Product: RND efflux system, membrane fusion protein CmeA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MKLFQKNTILALGVVLLLTACSKEEAPKIQMPPQPVTTMSAKSEDLPLSFTYPAKLVSDYDVIIKPQVSGVIENKLFKAG
DKVKKGQTLFIIEQDKFKASVDSAYGQALMAKATFENASKDFNRSKALFSKSAISQKEYDSSLATFNNSKASLASARAQL
ANARIDLDHTEIKAPFDGTIGDALVNIGDYVSASTTELVRVTNLNPIYADFFISDTDKLNLVRNTQSGKWDLDSIHANLN
LNGETVQGKLYFIDSVIDANSGTVKAKAIFDNNNSTLLPGAFATITSEGFIQKNGFKVPQIAVKQNQNDVYVLLVKNGKV
EKSSVHISYQNNEYAIIDKGLQNGDKIILDNFKKIQVGSEVKEIGAQ

Sequences:

>Translated_367_residues
MKLFQKNTILALGVVLLLTACSKEEAPKIQMPPQPVTTMSAKSEDLPLSFTYPAKLVSDYDVIIKPQVSGVIENKLFKAG
DKVKKGQTLFIIEQDKFKASVDSAYGQALMAKATFENASKDFNRSKALFSKSAISQKEYDSSLATFNNSKASLASARAQL
ANARIDLDHTEIKAPFDGTIGDALVNIGDYVSASTTELVRVTNLNPIYADFFISDTDKLNLVRNTQSGKWDLDSIHANLN
LNGETVQGKLYFIDSVIDANSGTVKAKAIFDNNNSTLLPGAFATITSEGFIQKNGFKVPQIAVKQNQNDVYVLLVKNGKV
EKSSVHISYQNNEYAIIDKGLQNGDKIILDNFKKIQVGSEVKEIGAQ
>Mature_367_residues
MKLFQKNTILALGVVLLLTACSKEEAPKIQMPPQPVTTMSAKSEDLPLSFTYPAKLVSDYDVIIKPQVSGVIENKLFKAG
DKVKKGQTLFIIEQDKFKASVDSAYGQALMAKATFENASKDFNRSKALFSKSAISQKEYDSSLATFNNSKASLASARAQL
ANARIDLDHTEIKAPFDGTIGDALVNIGDYVSASTTELVRVTNLNPIYADFFISDTDKLNLVRNTQSGKWDLDSIHANLN
LNGETVQGKLYFIDSVIDANSGTVKAKAIFDNNNSTLLPGAFATITSEGFIQKNGFKVPQIAVKQNQNDVYVLLVKNGKV
EKSSVHISYQNNEYAIIDKGLQNGDKIILDNFKKIQVGSEVKEIGAQ

Specific function: Cell membrane lipoprotein, involved in cell membrane permeability to hydrophobic compounds such as antibiotics, dyes and detergents [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789665, Length=354, Percent_Identity=30.7909604519774, Blast_Score=157, Evalue=9e-40,
Organism=Escherichia coli, GI1789929, Length=360, Percent_Identity=32.5, Blast_Score=154, Evalue=7e-39,
Organism=Escherichia coli, GI1786668, Length=364, Percent_Identity=31.043956043956, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI87082036, Length=345, Percent_Identity=28.1159420289855, Blast_Score=140, Evalue=9e-35,
Organism=Escherichia coli, GI1789637, Length=179, Percent_Identity=31.2849162011173, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 40092; Mature: 40092

Theoretical pI: Translated: 8.76; Mature: 8.76

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLFQKNTILALGVVLLLTACSKEEAPKIQMPPQPVTTMSAKSEDLPLSFTYPAKLVSDY
CCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCCCCEEEECCHHHHCCC
DVIIKPQVSGVIENKLFKAGDKVKKGQTLFIIEQDKFKASVDSAYGQALMAKATFENASK
EEEEECCHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHHEEEHHHHHHCHH
DFNRSKALFSKSAISQKEYDSSLATFNNSKASLASARAQLANARIDLDHTEIKAPFDGTI
HHHHHHHHHHHHHCCHHHHCCHHHHCCCCHHHHHHHHHHHHHCEEECCHHEECCCCCCCH
GDALVNIGDYVSASTTELVRVTNLNPIYADFFISDTDKLNLVRNTQSGKWDLDSIHANLN
HHHHHHCCCCCCCCCEEEEEEECCCCEEEEEEECCCCCEEEEECCCCCCEEHEEEEEEEE
LNGETVQGKLYFIDSVIDANSGTVKAKAIFDNNNSTLLPGAFATITSEGFIQKNGFKVPQ
ECCCEEEEEEEEEEEEEECCCCEEEEEEEEECCCCEECCCCCEEECCCCEEECCCCCCCE
IAVKQNQNDVYVLLVKNGKVEKSSVHISYQNNEYAIIDKGLQNGDKIILDNFKKIQVGSE
EEEECCCCCEEEEEECCCCEEEEEEEEEEECCEEEEEECCCCCCCEEEECCCEEEECCCH
VKEIGAQ
HHHHCCC
>Mature Secondary Structure
MKLFQKNTILALGVVLLLTACSKEEAPKIQMPPQPVTTMSAKSEDLPLSFTYPAKLVSDY
CCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCCCCEEEECCHHHHCCC
DVIIKPQVSGVIENKLFKAGDKVKKGQTLFIIEQDKFKASVDSAYGQALMAKATFENASK
EEEEECCHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHHEEEHHHHHHCHH
DFNRSKALFSKSAISQKEYDSSLATFNNSKASLASARAQLANARIDLDHTEIKAPFDGTI
HHHHHHHHHHHHHCCHHHHCCHHHHCCCCHHHHHHHHHHHHHCEEECCHHEECCCCCCCH
GDALVNIGDYVSASTTELVRVTNLNPIYADFFISDTDKLNLVRNTQSGKWDLDSIHANLN
HHHHHHCCCCCCCCCEEEEEEECCCCEEEEEEECCCCCEEEEECCCCCCEEHEEEEEEEE
LNGETVQGKLYFIDSVIDANSGTVKAKAIFDNNNSTLLPGAFATITSEGFIQKNGFKVPQ
ECCCEEEEEEEEEEEEEECCCCEEEEEEEEECCCCEECCCCCEEECCCCEEECCCCCCCE
IAVKQNQNDVYVLLVKNGKVEKSSVHISYQNNEYAIIDKGLQNGDKIILDNFKKIQVGSE
EEEECCCCCEEEEEECCCCEEEEEEEEEEECCEEEEEECCCCCCCEEEECCCEEEECCCH
VKEIGAQ
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8196548 [H]