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The map label for this gene is fliN [H]

Identifier: 57237406

GI number: 57237406

Start: 358928

End: 359236

Strand: Direct

Name: fliN [H]

Synonym: CJE0400

Alternate gene names: 57237406

Gene position: 358928-359236 (Clockwise)

Preceding gene: 57237405

Following gene: 57237407

Centisome position: 20.19

GC content: 31.72

Gene sequence:

>309_bases
ATGAGCGATGATATAGAGTTTAACATTCATCATGGGCTTTTACAATCTTATGAAGATATCTTAGATATTACAGTAGATTT
TGTAAGCGAGCTTGGAACTACAAATATGAGTGTAGCGGAGCTTTTAAAACTTGAAGTAGGTTCGGTTATTGATCTTGAAA
AGCCTGCTGGAGAAAGCGTTGAGCTTTATATCAATAAAAGAATTTTTGGCAAAGGCGAGGTTATGGTTTATGAAAAAAAT
CTCGCTATAAGAATTAATGAAATTTTGGATTCTAAGACTGTGCTTCAATACTTTAAAAAAGAAATTTAA

Upstream 100 bases:

>100_bases
CGGAATATTTAGGAAAAGTTGATTTTTCTGAAATAGTTCTAGATGAAGCTTTAACTTATAAATTTGAAAATTGTTATTTT
AGAATAGGATATTGCAAATA

Downstream 100 bases:

>100_bases
ATGAGATTGCTAGTATTATTTTTTTTAATTTTACCTTTATATAGTGTTGAGCTGATTAGTTTTAATATCTACGATAGAAA
TGATCGAGTAGATTTAATGC

Product: flagellar motor switch protein

Products: NA

Alternate protein names: CheY-P phosphatase FliY [H]

Number of amino acids: Translated: 102; Mature: 101

Protein sequence:

>102_residues
MSDDIEFNIHHGLLQSYEDILDITVDFVSELGTTNMSVAELLKLEVGSVIDLEKPAGESVELYINKRIFGKGEVMVYEKN
LAIRINEILDSKTVLQYFKKEI

Sequences:

>Translated_102_residues
MSDDIEFNIHHGLLQSYEDILDITVDFVSELGTTNMSVAELLKLEVGSVIDLEKPAGESVELYINKRIFGKGEVMVYEKN
LAIRINEILDSKTVLQYFKKEI
>Mature_101_residues
SDDIEFNIHHGLLQSYEDILDITVDFVSELGTTNMSVAELLKLEVGSVIDLEKPAGESVELYINKRIFGKGEVMVYEKNL
AIRINEILDSKTVLQYFKKEI

Specific function: Component of the flagellar switch. Binds CheY-P and increases its hydrolysis rate in vitro. May function constitutively to remove CheY-P around the flagellar switch to maintain an optimal level of CheY-P whereas CheC may function after addition of an attr

COG id: COG1886

COG function: function code NU; Flagellar motor switch/type III secretory pathway protein

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Cytoplasmic side. Note=The switch is localized at the base of the flagellar motor, in or near the C-ring, attached to the MS-ring (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FliN/MopA/SpaO family [H]

Homologues:

Organism=Escherichia coli, GI1788256, Length=90, Percent_Identity=35.5555555555556, Blast_Score=62, Evalue=1e-11,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007597
- InterPro:   IPR012826
- InterPro:   IPR001172
- InterPro:   IPR001543 [H]

Pfam domain/function: PF04509 CheC; PF01052 SpoA [H]

EC number: NA

Molecular weight: Translated: 11609; Mature: 11477

Theoretical pI: Translated: 4.28; Mature: 4.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDDIEFNIHHGLLQSYEDILDITVDFVSELGTTNMSVAELLKLEVGSVIDLEKPAGESV
CCCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEECCCCCCCCE
ELYINKRIFGKGEVMVYEKNLAIRINEILDSKTVLQYFKKEI
EEEEEEEEECCCCEEEEECCCEEEEHHHHCHHHHHHHHHHCC
>Mature Secondary Structure 
SDDIEFNIHHGLLQSYEDILDITVDFVSELGTTNMSVAELLKLEVGSVIDLEKPAGESV
CCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEECCCCCCCCE
ELYINKRIFGKGEVMVYEKNLAIRINEILDSKTVLQYFKKEI
EEEEEEEEECCCCEEEEECCCEEEEHHHHCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1447979; 9384377; 1898932 [H]