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The map label for this gene is rihA [H]

Identifier: 57237391

GI number: 57237391

Start: 345725

End: 346732

Strand: Direct

Name: rihA [H]

Synonym: CJE0385

Alternate gene names: 57237391

Gene position: 345725-346732 (Clockwise)

Preceding gene: 57237390

Following gene: 57237398

Centisome position: 19.45

GC content: 32.54

Gene sequence:

>1008_bases
ATGAGACTTATCTTAGACACGGACATAGGCAATGCTATTGCAGGAGCAAATACTGATGATGGTTTAGCACTTGCTTTGAT
TTTGTCATCTAAAGAAATCAAACTTGAAATGCTAAGCACAGTTTGCGGTAATGTTCCAAGCCTTGTTGCCTATAGTGTGG
CTAAGGATTTATTTAAACGTTTAAATTTAAATATCCCCGTTTATTTGGGAGCGAATGAAGCCCTAAAAGAACCAAGTAAA
GCTTGGCGTCAAAGACTTGATGAAAGTGTTAAAAATTTTAAACTTGAATATCTTTGGGAAAATATAAAAAGTCCTGAAAT
TTTAGAAAATATAAATCCTGATGCGATTTTTAAAATGGGTGAGTTGGTGAGTAAAAATCCTAAAGAAATTTCTATTTGTG
CGATAGGACCTTTGACAAATATCGCTATGACTATGAAAATTTTTAAAGATTTTGATATAAATCTTAAAGAGCTTTTTATT
ATGGGCGGCAGTTTTGATATGCCTTATTACACTAAAGATACAAATTTTGGTTTTGATCCTGAGGCGGCTAGTATAGTGCT
AAATTCAAGAGCTAAAATTACACTCGTACCTTATAATGCAACCATGCAGACTTTATTAACACACGAGGATTTAAAAGAGT
TGCAAGGTAAGAATATTTTATGTGATTTTATCGTTGAAACTTTAGGTGTTTGGATTGATTATGCCTCTAAAACACGTGGT
ACAAAGGGAACTTGGATACATGATGCTTTAACGATAGCTTACGCATTAGATTCTAGTATTGCAGATTTTGATGAGTGTTA
TGCTGATGTTATTTGCGATAGTTCTTTGGCAAGAGGTATGAGTTGGCGTTGTTTTAAAGAACCAAAAATGAGTATGGGGG
TGGATTTAAGCACTAAAAATTGTGTTAAAATTCTAAAAAATGTAGACAATGCAAAGCTTTTAAAACTTATAAAAGAGAAG
TTTTTAAAAGGAGTTTGCTATGAAAATTATGAAAGCATAACGACTTAA

Upstream 100 bases:

>100_bases
TCGCGACATATTTTGTATTAACTGCAGGTATAGGTTTTGTGACTACATTTTTTGCGCCAGAAACTAGAGGTAGAGATTTA
AATAAAATAGAAGATGCGAT

Downstream 100 bases:

>100_bases
TTTTCCAAGTAAGATAAAAAATATAGTTTTTAAAAAAGAATATTTCGCAAAACCAAGTCCCAAGGCAAAAAGATCTCCTA
CTAAAGGTAAAAAAGTAAAA

Product: inosine-uridine preferring nucleoside hydrolase

Products: uracil; ribose; cytosine [C]

Alternate protein names: Cytidine/uridine-specific hydrolase [H]

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MRLILDTDIGNAIAGANTDDGLALALILSSKEIKLEMLSTVCGNVPSLVAYSVAKDLFKRLNLNIPVYLGANEALKEPSK
AWRQRLDESVKNFKLEYLWENIKSPEILENINPDAIFKMGELVSKNPKEISICAIGPLTNIAMTMKIFKDFDINLKELFI
MGGSFDMPYYTKDTNFGFDPEAASIVLNSRAKITLVPYNATMQTLLTHEDLKELQGKNILCDFIVETLGVWIDYASKTRG
TKGTWIHDALTIAYALDSSIADFDECYADVICDSSLARGMSWRCFKEPKMSMGVDLSTKNCVKILKNVDNAKLLKLIKEK
FLKGVCYENYESITT

Sequences:

>Translated_335_residues
MRLILDTDIGNAIAGANTDDGLALALILSSKEIKLEMLSTVCGNVPSLVAYSVAKDLFKRLNLNIPVYLGANEALKEPSK
AWRQRLDESVKNFKLEYLWENIKSPEILENINPDAIFKMGELVSKNPKEISICAIGPLTNIAMTMKIFKDFDINLKELFI
MGGSFDMPYYTKDTNFGFDPEAASIVLNSRAKITLVPYNATMQTLLTHEDLKELQGKNILCDFIVETLGVWIDYASKTRG
TKGTWIHDALTIAYALDSSIADFDECYADVICDSSLARGMSWRCFKEPKMSMGVDLSTKNCVKILKNVDNAKLLKLIKEK
FLKGVCYENYESITT
>Mature_335_residues
MRLILDTDIGNAIAGANTDDGLALALILSSKEIKLEMLSTVCGNVPSLVAYSVAKDLFKRLNLNIPVYLGANEALKEPSK
AWRQRLDESVKNFKLEYLWENIKSPEILENINPDAIFKMGELVSKNPKEISICAIGPLTNIAMTMKIFKDFDINLKELFI
MGGSFDMPYYTKDTNFGFDPEAASIVLNSRAKITLVPYNATMQTLLTHEDLKELQGKNILCDFIVETLGVWIDYASKTRG
TKGTWIHDALTIAYALDSSIADFDECYADVICDSSLARGMSWRCFKEPKMSMGVDLSTKNCVKILKNVDNAKLLKLIKEK
FLKGVCYENYESITT

Specific function: Hydrolyzes cytidine or uridine to ribose and cytosine or uracil, respectively [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786871, Length=317, Percent_Identity=23.3438485804416, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1788486, Length=284, Percent_Identity=22.1830985915493, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1786213, Length=278, Percent_Identity=24.8201438848921, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI32565474, Length=211, Percent_Identity=29.8578199052133, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24641837, Length=188, Percent_Identity=31.3829787234043, Blast_Score=71, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015910
- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022975 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: NA

Molecular weight: Translated: 37495; Mature: 37495

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLILDTDIGNAIAGANTDDGLALALILSSKEIKLEMLSTVCGNVPSLVAYSVAKDLFKR
CEEEEECCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
LNLNIPVYLGANEALKEPSKAWRQRLDESVKNFKLEYLWENIKSPEILENINPDAIFKMG
HCCCCEEEECCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCHHHHHHHH
ELVSKNPKEISICAIGPLTNIAMTMKIFKDFDINLKELFIMGGSFDMPYYTKDTNFGFDP
HHHCCCCCEEEEEEECCHHHHHHHHHHHHCCCCCHHHEEEECCCCCCCEEECCCCCCCCC
EAASIVLNSRAKITLVPYNATMQTLLTHEDLKELQGKNILCDFIVETLGVWIDYASKTRG
CHHEEEEECCCEEEEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC
TKGTWIHDALTIAYALDSSIADFDECYADVICDSSLARGMSWRCFKEPKMSMGVDLSTKN
CCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCHHHHCCCCCEEECCCCCCCCCCCCHHH
CVKILKNVDNAKLLKLIKEKFLKGVCYENYESITT
HHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHCCC
>Mature Secondary Structure
MRLILDTDIGNAIAGANTDDGLALALILSSKEIKLEMLSTVCGNVPSLVAYSVAKDLFKR
CEEEEECCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
LNLNIPVYLGANEALKEPSKAWRQRLDESVKNFKLEYLWENIKSPEILENINPDAIFKMG
HCCCCEEEECCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCHHHHHHHH
ELVSKNPKEISICAIGPLTNIAMTMKIFKDFDINLKELFIMGGSFDMPYYTKDTNFGFDP
HHHCCCCCEEEEEEECCHHHHHHHHHHHHCCCCCHHHEEEECCCCCCCEEECCCCCCCCC
EAASIVLNSRAKITLVPYNATMQTLLTHEDLKELQGKNILCDFIVETLGVWIDYASKTRG
CHHEEEEECCCEEEEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC
TKGTWIHDALTIAYALDSSIADFDECYADVICDSSLARGMSWRCFKEPKMSMGVDLSTKN
CCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCHHHHCCCCCEEECCCCCCCCCCCCHHH
CVKILKNVDNAKLLKLIKEKFLKGVCYENYESITT
HHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uridine; H2O; cytidine [C]

Specific reaction: uridine + H2O = uracil + ribose cytidine + H2O = cytosine + ribose [C]

General reaction: Pyrimidine-specific nucleoside hydrolase [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA