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The map label for this gene is panB [H]

Identifier: 57237349

GI number: 57237349

Start: 308107

End: 308931

Strand: Reverse

Name: panB [H]

Synonym: CJE0343

Alternate gene names: 57237349

Gene position: 308931-308107 (Counterclockwise)

Preceding gene: 57237351

Following gene: 57237348

Centisome position: 17.38

GC content: 34.55

Gene sequence:

>825_bases
ATGAGAAAAAGTATTCTTAGTTTTTTGGAAAAAAAAGGTAAAAATGAAAAAATAACCATGGTAAGTGCCTATGACTACCA
TAGTGCAAAAATTCTAGATAATTGCGATATAGATATCATATTGGTTGGAGATTCATTAGCTATGACAGTGCTTGGCATGC
AAGATACTTTAAGTGTGACCATGGATGAAATGTTGATCTTTACCAAAGCTGTTTCTCGCGGAGCTAAAAAGTCTTTCGTA
CTTGCGGATATGCCTTTCATGTCTTATCAAAGCTCGGATCGTGACGCTATCTTAAATGCCTCAAGATTTATCAAAGAAAG
CCATGCAAATGGGGTAAAAGTGGAAGGGGGTATAGAAATCGCAAGCAAGATTAAATTAATTTCCCAATCAGGAATACCCG
TCGTTGCTCACCTAGGACTAACCCCACAAGCTGTAAATATGCTAGGTGGATATAGAGTTCAAGGTAAAGATTTACAATCG
GCTCAAAAAATCATAGATGATGCAAAAGCTGTGCAAGATGCGGGTGCTTGTATGTTGGTTTTAGAATGCGTACCTGTAAA
ATTAGCACAAAAAATAAGCTCTATTTTAGAAATTCCAACAATCGGCATAGGTTCAGGTAAATACTGCGATGGACAAGTTT
TAGTTTATCATGATCTTTTAGGTTTAAATAAAGATTTTAAAGCAAAATTTGTAAAGCATTTTGATAAAATTGACCCTCAA
GTAGGAGTTGAAAAATATAGAGATGAAGTTAAAAGCGGTATTTTTCCTTCCGAAGAGCACAGCTTTGATTATTTAGATGA
TGAATTATTAGATAAATTATACTAA

Upstream 100 bases:

>100_bases
ATCGATTAAATATAACTTACTTTATCATTCATTTACTATTTATGAAAAATTATATGCTAGTTTTTTGCTTATTATTTTAT
AGCTAAACGAGGTATTTTAA

Downstream 100 bases:

>100_bases
GGATACAAATATGGAAGTCATTACTTCTATCAAAGAAGCAAAACAAACTGTAAAAAATTGGAAATCACATAACCTTAGCA
TAGGCTATGTTCCAACCATG

Product: 3-methyl-2-oxobutanoate hydroxymethyltransferase

Products: NA

Alternate protein names: Ketopantoate hydroxymethyltransferase; KPHMT [H]

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MRKSILSFLEKKGKNEKITMVSAYDYHSAKILDNCDIDIILVGDSLAMTVLGMQDTLSVTMDEMLIFTKAVSRGAKKSFV
LADMPFMSYQSSDRDAILNASRFIKESHANGVKVEGGIEIASKIKLISQSGIPVVAHLGLTPQAVNMLGGYRVQGKDLQS
AQKIIDDAKAVQDAGACMLVLECVPVKLAQKISSILEIPTIGIGSGKYCDGQVLVYHDLLGLNKDFKAKFVKHFDKIDPQ
VGVEKYRDEVKSGIFPSEEHSFDYLDDELLDKLY

Sequences:

>Translated_274_residues
MRKSILSFLEKKGKNEKITMVSAYDYHSAKILDNCDIDIILVGDSLAMTVLGMQDTLSVTMDEMLIFTKAVSRGAKKSFV
LADMPFMSYQSSDRDAILNASRFIKESHANGVKVEGGIEIASKIKLISQSGIPVVAHLGLTPQAVNMLGGYRVQGKDLQS
AQKIIDDAKAVQDAGACMLVLECVPVKLAQKISSILEIPTIGIGSGKYCDGQVLVYHDLLGLNKDFKAKFVKHFDKIDPQ
VGVEKYRDEVKSGIFPSEEHSFDYLDDELLDKLY
>Mature_274_residues
MRKSILSFLEKKGKNEKITMVSAYDYHSAKILDNCDIDIILVGDSLAMTVLGMQDTLSVTMDEMLIFTKAVSRGAKKSFV
LADMPFMSYQSSDRDAILNASRFIKESHANGVKVEGGIEIASKIKLISQSGIPVVAHLGLTPQAVNMLGGYRVQGKDLQS
AQKIIDDAKAVQDAGACMLVLECVPVKLAQKISSILEIPTIGIGSGKYCDGQVLVYHDLLGLNKDFKAKFVKHFDKIDPQ
VGVEKYRDEVKSGIFPSEEHSFDYLDDELLDKLY

Specific function: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate [H]

COG id: COG0413

COG function: function code H; Ketopantoate hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the panB family [H]

Homologues:

Organism=Escherichia coli, GI1786326, Length=265, Percent_Identity=43.3962264150943, Blast_Score=233, Evalue=1e-62,
Organism=Saccharomyces cerevisiae, GI6319653, Length=261, Percent_Identity=40.6130268199234, Blast_Score=177, Evalue=1e-45,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003700
- InterPro:   IPR015813 [H]

Pfam domain/function: PF02548 Pantoate_transf [H]

EC number: =2.1.2.11 [H]

Molecular weight: Translated: 30159; Mature: 30159

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKSILSFLEKKGKNEKITMVSAYDYHSAKILDNCDIDIILVGDSLAMTVLGMQDTLSVT
CHHHHHHHHHHCCCCCEEEEEEECCCCCCEEECCCCEEEEEECCHHHHHHHCCHHHHHHH
MDEMLIFTKAVSRGAKKSFVLADMPFMSYQSSDRDAILNASRFIKESHANGVKVEGGIEI
HHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEECCCHHH
ASKIKLISQSGIPVVAHLGLTPQAVNMLGGYRVQGKDLQSAQKIIDDAKAVQDAGACMLV
HHHHHHHHCCCCCEEEECCCCHHHHHHHCCEEECCCCHHHHHHHHHHHHHHHHCCHHEEH
LECVPVKLAQKISSILEIPTIGIGSGKYCDGQVLVYHDLLGLNKDFKAKFVKHFDKIDPQ
HHHHHHHHHHHHHHHHHCCCEECCCCCCCCCEEEEEEHHHCCCCHHHHHHHHHHHHCCCC
VGVEKYRDEVKSGIFPSEEHSFDYLDDELLDKLY
CCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCC
>Mature Secondary Structure
MRKSILSFLEKKGKNEKITMVSAYDYHSAKILDNCDIDIILVGDSLAMTVLGMQDTLSVT
CHHHHHHHHHHCCCCCEEEEEEECCCCCCEEECCCCEEEEEECCHHHHHHHCCHHHHHHH
MDEMLIFTKAVSRGAKKSFVLADMPFMSYQSSDRDAILNASRFIKESHANGVKVEGGIEI
HHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEECCCHHH
ASKIKLISQSGIPVVAHLGLTPQAVNMLGGYRVQGKDLQSAQKIIDDAKAVQDAGACMLV
HHHHHHHHCCCCCEEEECCCCHHHHHHHCCEEECCCCHHHHHHHHHHHHHHHHCCHHEEH
LECVPVKLAQKISSILEIPTIGIGSGKYCDGQVLVYHDLLGLNKDFKAKFVKHFDKIDPQ
HHHHHHHHHHHHHHHHHCCCEECCCCCCCCCEEEEEEHHHCCCCHHHHHHHHHHHHCCCC
VGVEKYRDEVKSGIFPSEEHSFDYLDDELLDKLY
CCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA