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The map label for this gene is greA

Identifier: 57237342

GI number: 57237342

Start: 301246

End: 301731

Strand: Reverse

Name: greA

Synonym: CJE0335

Alternate gene names: 57237342

Gene position: 301731-301246 (Counterclockwise)

Preceding gene: 57237343

Following gene: 57237341

Centisome position: 16.97

GC content: 35.8

Gene sequence:

>486_bases
ATGCAAAAAGAACCAATGAGTCAATTTGGCTATGATAAGTTAGCCGCAGAATTAAAAGATTTAAAAGACAATCAACGCCC
TGCCGTTGTAATAGAAATCGATACAGCAAGAAGTCATGGGGATTTGAAAGAAAATGCTGAATACCATGCTGCAAGAGAAA
AGCAAGCTTTGATAGAAAGTCGTATTGCAGAGCTTAGTGACTTGCTAGCAAGAGCTCAAGTGATCGATCCTTCAAGCTAT
GAGCACGATAGTGTGAAATTTGGTTCAAGTGTTGTGATCATGGATTTGGATACAGAAAAAGAAAGCAAATATACTCTAGT
AGGAATTTGCGAAGGAAATTTAGATAAGGGTTATATTTCCATCGCATCTCCAATAGCAAAAGCTATGCTTGGAAAAAAAG
AAGGAGATGATTTTAAAGTGCGTTTGCCAAAAGGTGAAAGTGAATTTGAAATCCTTTCTATAAATTATGAGCCTTTGAAA
TTTTAA

Upstream 100 bases:

>100_bases
AGGCATTTTTTGCTAAAGTTGATTTTTTAAAAGAATATTTACAATTTGGAAGTGCTAAAAATTTAGCAAAAATCCTAAAC
GAAATATAAAAGGATAAATC

Downstream 100 bases:

>100_bases
GGATAAAAAATTTTACTACAAAATGGAGCGATTTTAATCGCAGATGCTCATGAAAATGAACAAAGAAAGGGATTTTGGGA
ATTTTTACAAGCCTTAAAAA

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA

Number of amino acids: Translated: 161; Mature: 161

Protein sequence:

>161_residues
MQKEPMSQFGYDKLAAELKDLKDNQRPAVVIEIDTARSHGDLKENAEYHAAREKQALIESRIAELSDLLARAQVIDPSSY
EHDSVKFGSSVVIMDLDTEKESKYTLVGICEGNLDKGYISIASPIAKAMLGKKEGDDFKVRLPKGESEFEILSINYEPLK
F

Sequences:

>Translated_161_residues
MQKEPMSQFGYDKLAAELKDLKDNQRPAVVIEIDTARSHGDLKENAEYHAAREKQALIESRIAELSDLLARAQVIDPSSY
EHDSVKFGSSVVIMDLDTEKESKYTLVGICEGNLDKGYISIASPIAKAMLGKKEGDDFKVRLPKGESEFEILSINYEPLK
F
>Mature_161_residues
MQKEPMSQFGYDKLAAELKDLKDNQRPAVVIEIDTARSHGDLKENAEYHAAREKQALIESRIAELSDLLARAQVIDPSSY
EHDSVKFGSSVVIMDLDTEKESKYTLVGICEGNLDKGYISIASPIAKAMLGKKEGDDFKVRLPKGESEFEILSINYEPLK
F

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family

Homologues:

Organism=Escherichia coli, GI87082225, Length=157, Percent_Identity=43.9490445859873, Blast_Score=137, Evalue=4e-34,
Organism=Escherichia coli, GI87082261, Length=156, Percent_Identity=33.974358974359, Blast_Score=81, Evalue=3e-17,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): GREA_CAMJ8 (A8FK75)

Other databases:

- EMBL:   CP000814
- RefSeq:   YP_001481839.1
- ProteinModelPortal:   A8FK75
- SMR:   A8FK75
- STRING:   A8FK75
- GeneID:   5617097
- GenomeReviews:   CP000814_GR
- KEGG:   cju:C8J_0263
- eggNOG:   COG0782
- HOGENOM:   HBG753387
- OMA:   HNEGRIA
- ProtClustDB:   PRK00226
- BioCyc:   CJEJ407148:C8J_0263-MONOMER
- GO:   GO:0006350
- HAMAP:   MF_00105
- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691
- Gene3D:   G3DSA:1.10.287.180
- Gene3D:   G3DSA:3.10.50.30
- TIGRFAMs:   TIGR01462

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N; SSF46557 GreA_GreB

EC number: NA

Molecular weight: Translated: 18011; Mature: 18011

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS00829 GREAB_1; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKEPMSQFGYDKLAAELKDLKDNQRPAVVIEIDTARSHGDLKENAEYHAAREKQALIES
CCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHH
RIAELSDLLARAQVIDPSSYEHDSVKFGSSVVIMDLDTEKESKYTLVGICEGNLDKGYIS
HHHHHHHHHHHHHCCCCCCCCCCCEECCCEEEEEEECCCCCCCEEEEEEEECCCCCCEEE
IASPIAKAMLGKKEGDDFKVRLPKGESEFEILSINYEPLKF
HHHHHHHHHHCCCCCCCEEEECCCCCCCEEEEEECCCCCCC
>Mature Secondary Structure
MQKEPMSQFGYDKLAAELKDLKDNQRPAVVIEIDTARSHGDLKENAEYHAAREKQALIES
CCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHH
RIAELSDLLARAQVIDPSSYEHDSVKFGSSVVIMDLDTEKESKYTLVGICEGNLDKGYIS
HHHHHHHHHHHHHCCCCCCCCCCCEECCCEEEEEEECCCCCCCEEEEEEEECCCCCCEEE
IASPIAKAMLGKKEGDDFKVRLPKGESEFEILSINYEPLKF
HHHHHHHHHHCCCCCCCEEEECCCCCCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA