Click here to switch to the map view.

The map label for this gene is pyrC [H]

Identifier: 57237316

GI number: 57237316

Start: 271412

End: 272407

Strand: Direct

Name: pyrC [H]

Synonym: CJE0309

Alternate gene names: 57237316

Gene position: 271412-272407 (Clockwise)

Preceding gene: 57237315

Following gene: 57237317

Centisome position: 15.27

GC content: 32.43

Gene sequence:

>996_bases
ATGAAACTTAAAAACCCTTTGGATATGCATTTACATTTACGCGATGAAAGTATGCTTGAGCTTGTAGCTCCTTTTAGTGC
AAAAGACTTTAAGGCAGGGGTTATCATGCCAAATTTAATCACCCCACTTACTGAAATAGATGTATTAAAAGCTTATAAAG
AACGCATTTTAAAAGCATGTAAAAATGAAGATTTTACGCCTTTGATGACTTTGTTTTTCAAAGACTATGATGAGCAATTT
TTAGAAAAAGCTAAAGACGAGCTTTTTGCTATCAAACTTTATCCTGCAGGTATCACTACCAACTCAGATAATGGAATTTC
AAGTTTTGATATAGAAAAGTTAAAACCTACATTAATTACTATGAGCGAATTAAACATACCCTTACTTGTGCATGGAGAAA
CCAATGATTTTGTCATGGATAGAGAAGCAAATTTTGCCAAAATATATGAAAAATTAGCTAAAAACTTTCCAAAATTAAAA
ATTGTAATGGAGCATATTACCACTAAGGTTTTGTGTGATTTATTAAAAGATTATGAAAATTTATACGCAACTATTACTTT
GCATCATTTAATGATAACGCTTGATGATGTAGTGGGTGGTAAAATGGATCCACATTTATTTTGCAAGCCTATTGCAAAAC
GCTATGAAGATAAAGACGCTTTATGTGGGCTTGCTTTTAGTGGCTATGAAAAGGCTATGTTTGGAAGCGATAGCGCGCCT
CATCCATTGCACACTAAAGAATGTTGTGGTTGTGCGGCTGGAGTTTTTAGCGCACCGGTGATTTTACCTGTATTAGTTGA
ACTTTTTGAAAAATATTCAAATGAAACAAATTTGCAAAAATTTATTTCAGATAATGCTTGCAAAATCCATAATCTTAAAT
TTGAAAATGACAAAATTATCACTTTAGAAAAACAAGAATGGCAAGTAGCGCAAAAATACGGCGATGTAGTGCCATTTATG
GCAGGGAAAACTTTAAATTTCAAAGTCGCAAATTAA

Upstream 100 bases:

>100_bases
GCAAGCCCACTATCATCGCTACTTTTAAATTTTTAGAAGAAAAAAAGCTTTTTAAGCGTCTTAAAAATGGATTTTATCAG
CTAAAAATATAGGAGATAAA

Downstream 100 bases:

>100_bases
AGGAAATTAATGCTAAAAACTTATTAGAAAACATAAAAGATATTTCTATTAATGATAAAGAACATACCCATAGAACAGCT
TTGCAAAATTTATTGCAAGC

Product: dihydroorotase

Products: NA

Alternate protein names: DHOase [H]

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MKLKNPLDMHLHLRDESMLELVAPFSAKDFKAGVIMPNLITPLTEIDVLKAYKERILKACKNEDFTPLMTLFFKDYDEQF
LEKAKDELFAIKLYPAGITTNSDNGISSFDIEKLKPTLITMSELNIPLLVHGETNDFVMDREANFAKIYEKLAKNFPKLK
IVMEHITTKVLCDLLKDYENLYATITLHHLMITLDDVVGGKMDPHLFCKPIAKRYEDKDALCGLAFSGYEKAMFGSDSAP
HPLHTKECCGCAAGVFSAPVILPVLVELFEKYSNETNLQKFISDNACKIHNLKFENDKIITLEKQEWQVAQKYGDVVPFM
AGKTLNFKVAN

Sequences:

>Translated_331_residues
MKLKNPLDMHLHLRDESMLELVAPFSAKDFKAGVIMPNLITPLTEIDVLKAYKERILKACKNEDFTPLMTLFFKDYDEQF
LEKAKDELFAIKLYPAGITTNSDNGISSFDIEKLKPTLITMSELNIPLLVHGETNDFVMDREANFAKIYEKLAKNFPKLK
IVMEHITTKVLCDLLKDYENLYATITLHHLMITLDDVVGGKMDPHLFCKPIAKRYEDKDALCGLAFSGYEKAMFGSDSAP
HPLHTKECCGCAAGVFSAPVILPVLVELFEKYSNETNLQKFISDNACKIHNLKFENDKIITLEKQEWQVAQKYGDVVPFM
AGKTLNFKVAN
>Mature_331_residues
MKLKNPLDMHLHLRDESMLELVAPFSAKDFKAGVIMPNLITPLTEIDVLKAYKERILKACKNEDFTPLMTLFFKDYDEQF
LEKAKDELFAIKLYPAGITTNSDNGISSFDIEKLKPTLITMSELNIPLLVHGETNDFVMDREANFAKIYEKLAKNFPKLK
IVMEHITTKVLCDLLKDYENLYATITLHHLMITLDDVVGGKMDPHLFCKPIAKRYEDKDALCGLAFSGYEKAMFGSDSAP
HPLHTKECCGCAAGVFSAPVILPVLVELFEKYSNETNLQKFISDNACKIHNLKFENDKIITLEKQEWQVAQKYGDVVPFM
AGKTLNFKVAN

Specific function: Pyrimidine biosynthesis; third step. [C]

COG id: COG0418

COG function: function code F; Dihydroorotase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHOase family. Type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787301, Length=343, Percent_Identity=40.8163265306122, Blast_Score=238, Evalue=6e-64,
Organism=Saccharomyces cerevisiae, GI6323452, Length=357, Percent_Identity=35.0140056022409, Blast_Score=176, Evalue=6e-45,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006992
- InterPro:   IPR004721
- InterPro:   IPR002195 [H]

Pfam domain/function: PF04909 Amidohydro_2 [H]

EC number: =3.5.2.3 [H]

Molecular weight: Translated: 37570; Mature: 37570

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS00483 DIHYDROOROTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLKNPLDMHLHLRDESMLELVAPFSAKDFKAGVIMPNLITPLTEIDVLKAYKERILKAC
CCCCCCCCEEEEECCHHHHHHHCCCCCCCCCCCEECCHHHCHHHHHHHHHHHHHHHHHHH
KNEDFTPLMTLFFKDYDEQFLEKAKDELFAIKLYPAGITTNSDNGISSFDIEKLKPTLIT
CCCCCCHHHHHHHHHHHHHHHHHHHHCEEEEEEEECCEECCCCCCCCCCCHHHCCCCEEE
MSELNIPLLVHGETNDFVMDREANFAKIYEKLAKNFPKLKIVMEHITTKVLCDLLKDYEN
EECCCCEEEEECCCCCEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
LYATITLHHLMITLDDVVGGKMDPHLFCKPIAKRYEDKDALCGLAFSGYEKAMFGSDSAP
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHEEEECCCCHHHHCCCCCCC
HPLHTKECCGCAAGVFSAPVILPVLVELFEKYSNETNLQKFISDNACKIHNLKFENDKII
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCEEEEEEEECCCEEE
TLEKQEWQVAQKYGDVVPFMAGKTLNFKVAN
EEEHHHHHHHHHHCCCCCCCCCCEEEEEECC
>Mature Secondary Structure
MKLKNPLDMHLHLRDESMLELVAPFSAKDFKAGVIMPNLITPLTEIDVLKAYKERILKAC
CCCCCCCCEEEEECCHHHHHHHCCCCCCCCCCCEECCHHHCHHHHHHHHHHHHHHHHHHH
KNEDFTPLMTLFFKDYDEQFLEKAKDELFAIKLYPAGITTNSDNGISSFDIEKLKPTLIT
CCCCCCHHHHHHHHHHHHHHHHHHHHCEEEEEEEECCEECCCCCCCCCCCHHHCCCCEEE
MSELNIPLLVHGETNDFVMDREANFAKIYEKLAKNFPKLKIVMEHITTKVLCDLLKDYEN
EECCCCEEEEECCCCCEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
LYATITLHHLMITLDDVVGGKMDPHLFCKPIAKRYEDKDALCGLAFSGYEKAMFGSDSAP
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHEEEECCCCHHHHCCCCCCC
HPLHTKECCGCAAGVFSAPVILPVLVELFEKYSNETNLQKFISDNACKIHNLKFENDKII
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCEEEEEEEECCCEEE
TLEKQEWQVAQKYGDVVPFMAGKTLNFKVAN
EEEHHHHHHHHHHCCCCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12810954 [H]