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The map label for this gene is nrdF [H]

Identifier: 57237291

GI number: 57237291

Start: 250676

End: 251698

Strand: Reverse

Name: nrdF [H]

Synonym: CJE0282

Alternate gene names: 57237291

Gene position: 251698-250676 (Counterclockwise)

Preceding gene: 57237292

Following gene: 57237290

Centisome position: 14.16

GC content: 31.87

Gene sequence:

>1023_bases
ATGCAAAGAAAAAGAATTTACAATCCAAACTCAAACGAAACCCTAGGAGATCGTAAAATTTTTGATGGTAACCCACATGG
AATTTTAAATTTCACTAAGGCAAAATATACCTGGGCTTTAAAACTTTGGGATTTAATGGAGGCAAATACTTGGTTTCCAA
AAGAAGTGGATACAACTAAAGATGCACTTGATTATCGCTGTAATTTAACTGTAGGTGAAAAAAGAATGTATGATTTGGTT
TGGAGTCAGCTTATTTCTATGGATAGTTTTCAAACCAATAATTTAGCAGATAATATCAACCCTTACATCACAGCTCCTGA
AATCAATGCGGTTTTGGCACGCCAAGCTTATGAAGAGGCAAACCATTCAAAATCTTACGCTGTTATGGTAGAAGCGATTT
GCGATAATACAGATTTGATTTATGAAATGGAAAAGCATGATGAAACTTTGCGTGAAAAAAATGACTTTATTTCAAGCATT
TATGAAGAATTAGCAGGCGATGTAGATGATAATAAACTTCTTTTGGCTATGGTGGCAAATCAAATTTTAGAAGGAGTATA
TTTTTATAGTGGTTTTACTGCCATTTACGCACTTGCACGTGCAGGAAAAATGCTAGGTTCAGCACAAATGATCCGCTTTA
TCCAAAGAGATGAAATCACACATCTTTTATTATTTCAAAATATGATCAATTCAGTACGCAAAGAAAGACCTGATTTATTC
CATGATGAAAATATCAATAAAATTTATGATATGTTTAAAAAAGCTGGAGATCTTGAGATCAAATGGGGTAAATACATCAC
TCAAAATCAAATCATGGGCTTTACTGATGATATCATAGAAGAATATATCCATTATCTTGTAGATCAAAGATTAAGTGCGA
TCAATTTAGATAAGTTATATAATGCAAAACATCCTATTAAATGGGTAGATGATTTTTCAAAATTTAACGATCAAAAGAGC
AATTTCTTTGAAAGTAAAGTAACGAATTATTCTAAGGGTAGTATAAGCTTTGATGACTTCTAA

Upstream 100 bases:

>100_bases
TTGGTTTATTTGTCAGTTTTGTAAGAAAAGATCGTTTAAATTTACACGATATTTTAACTCAAAGTTGTATAGCTACTAAA
AGCATAAAGAAAGGTTAAAA

Downstream 100 bases:

>100_bases
AACTTCTTTAGCCTTACTTTGTGATTTTTATGAATTTACAATGTCTCAAGGCTATTTCAAAAACAATAAAAAAGATCAAA
TTTGTTATTTTGATATATTT

Product: ribonucleotide-diphosphate reductase subunit beta

Products: NA

Alternate protein names: Ribonucleotide reductase small subunit [H]

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MQRKRIYNPNSNETLGDRKIFDGNPHGILNFTKAKYTWALKLWDLMEANTWFPKEVDTTKDALDYRCNLTVGEKRMYDLV
WSQLISMDSFQTNNLADNINPYITAPEINAVLARQAYEEANHSKSYAVMVEAICDNTDLIYEMEKHDETLREKNDFISSI
YEELAGDVDDNKLLLAMVANQILEGVYFYSGFTAIYALARAGKMLGSAQMIRFIQRDEITHLLLFQNMINSVRKERPDLF
HDENINKIYDMFKKAGDLEIKWGKYITQNQIMGFTDDIIEEYIHYLVDQRLSAINLDKLYNAKHPIKWVDDFSKFNDQKS
NFFESKVTNYSKGSISFDDF

Sequences:

>Translated_340_residues
MQRKRIYNPNSNETLGDRKIFDGNPHGILNFTKAKYTWALKLWDLMEANTWFPKEVDTTKDALDYRCNLTVGEKRMYDLV
WSQLISMDSFQTNNLADNINPYITAPEINAVLARQAYEEANHSKSYAVMVEAICDNTDLIYEMEKHDETLREKNDFISSI
YEELAGDVDDNKLLLAMVANQILEGVYFYSGFTAIYALARAGKMLGSAQMIRFIQRDEITHLLLFQNMINSVRKERPDLF
HDENINKIYDMFKKAGDLEIKWGKYITQNQIMGFTDDIIEEYIHYLVDQRLSAINLDKLYNAKHPIKWVDDFSKFNDQKS
NFFESKVTNYSKGSISFDDF
>Mature_340_residues
MQRKRIYNPNSNETLGDRKIFDGNPHGILNFTKAKYTWALKLWDLMEANTWFPKEVDTTKDALDYRCNLTVGEKRMYDLV
WSQLISMDSFQTNNLADNINPYITAPEINAVLARQAYEEANHSKSYAVMVEAICDNTDLIYEMEKHDETLREKNDFISSI
YEELAGDVDDNKLLLAMVANQILEGVYFYSGFTAIYALARAGKMLGSAQMIRFIQRDEITHLLLFQNMINSVRKERPDLF
HDENINKIYDMFKKAGDLEIKWGKYITQNQIMGFTDDIIEEYIHYLVDQRLSAINLDKLYNAKHPIKWVDDFSKFNDQKS
NFFESKVTNYSKGSISFDDF

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0208

COG function: function code F; Ribonucleotide reductase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase small chain family [H]

Homologues:

Organism=Homo sapiens, GI42544136, Length=310, Percent_Identity=23.2258064516129, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI289177074, Length=310, Percent_Identity=23.2258064516129, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI289177076, Length=296, Percent_Identity=23.3108108108108, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI4557845, Length=326, Percent_Identity=19.3251533742331, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI260064013, Length=326, Percent_Identity=19.3251533742331, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI1788567, Length=348, Percent_Identity=26.4367816091954, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1789031, Length=184, Percent_Identity=29.3478260869565, Blast_Score=80, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17551968, Length=325, Percent_Identity=24.3076923076923, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6322434, Length=310, Percent_Identity=24.5161290322581, Blast_Score=81, Evalue=2e-16,
Organism=Drosophila melanogaster, GI18079279, Length=302, Percent_Identity=23.1788079470199, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009078
- InterPro:   IPR012348
- InterPro:   IPR000358 [H]

Pfam domain/function: PF00268 Ribonuc_red_sm [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 39717; Mature: 39717

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRKRIYNPNSNETLGDRKIFDGNPHGILNFTKAKYTWALKLWDLMEANTWFPKEVDTTK
CCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCEEEEHHHHHHHHCCCCCCCCCCCHH
DALDYRCNLTVGEKRMYDLVWSQLISMDSFQTNNLADNINPYITAPEINAVLARQAYEEA
HHHCEEECEEECHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHH
NHSKSYAVMVEAICDNTDLIYEMEKHDETLREKNDFISSIYEELAGDVDDNKLLLAMVAN
CCCCHHHEEHHHHHCCCHHEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
QILEGVYFYSGFTAIYALARAGKMLGSAQMIRFIQRDEITHLLLFQNMINSVRKERPDLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
HDENINKIYDMFKKAGDLEIKWGKYITQNQIMGFTDDIIEEYIHYLVDQRLSAINLDKLY
CCCCHHHHHHHHHCCCCCEEECCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHH
NAKHPIKWVDDFSKFNDQKSNFFESKVTNYSKGSISFDDF
CCCCCCHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MQRKRIYNPNSNETLGDRKIFDGNPHGILNFTKAKYTWALKLWDLMEANTWFPKEVDTTK
CCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCEEEEHHHHHHHHCCCCCCCCCCCHH
DALDYRCNLTVGEKRMYDLVWSQLISMDSFQTNNLADNINPYITAPEINAVLARQAYEEA
HHHCEEECEEECHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHH
NHSKSYAVMVEAICDNTDLIYEMEKHDETLREKNDFISSIYEELAGDVDDNKLLLAMVAN
CCCCHHHEEHHHHHCCCHHEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
QILEGVYFYSGFTAIYALARAGKMLGSAQMIRFIQRDEITHLLLFQNMINSVRKERPDLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
HDENINKIYDMFKKAGDLEIKWGKYITQNQIMGFTDDIIEEYIHYLVDQRLSAINLDKLY
CCCCHHHHHHHHHCCCCCEEECCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHH
NAKHPIKWVDDFSKFNDQKSNFFESKVTNYSKGSISFDDF
CCCCCCHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]