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The map label for this gene is pcm [H]

Identifier: 57237288

GI number: 57237288

Start: 248081

End: 248710

Strand: Reverse

Name: pcm [H]

Synonym: CJE0279

Alternate gene names: 57237288

Gene position: 248710-248081 (Counterclockwise)

Preceding gene: 57237290

Following gene: 57237281

Centisome position: 13.99

GC content: 32.06

Gene sequence:

>630_bases
ATGAACGCATTTGAACAAAAAAGATGTCAAAATATGGCTGAAGAAATTGCACAAAAAGTTTTTATCAATGAAGAGCTTTT
TAATGCTTTTTGTCAAATTCCGCGTGAAATTTTTTCTCCTTTAAAAGCCCATGCTTATCGCCTTGATGCTTTGCCTTTAG
CAAATTCTCAATGGATTAGTTCACCTTTAACTGTAGCTAAAATGACTATGGCTTTAGATTTTAAAAATGCTGATAGTATT
TTAGAGATAGGTTGTGGAAGTGGCTATCAAGCTGCGATTTTAAGCAAAGTCATAAGGCGTGTTTTTACCATAGAACGCAT
AGAAAATTTAGCCAAAAAAGCCGCTCAAACTTTCAGAGAATTAGAACTTTTTAATATCAATGTAAAATTTGATGATGGAC
AAAATGGCTGGAAAAATTATGCTCCTTATGATAGGATTTTATTTTCTGCTTATGCGACACAAATTCCTGAAATTTTACTC
GATCAATTAAGCGATGATGGTATTTTAGTTGCTCCTATTTTACACAATGGCAAACAATTCATCACACGCCTTAGAAAAAA
TGGCACAAATTTACAAAAAGAAATTTTAGAAGAATGTTTATTTGTGCCTATAGTAGATGGGAAAGAATAA

Upstream 100 bases:

>100_bases
TTTTATAAGCATTTTTTTCCTTTTTATTTAAGTATAGCTAAGTTTAACTCTATTTCAACTTTTTTAGTTACAATTTTTAT
TTAAATTTATAGGAGAAGTA

Downstream 100 bases:

>100_bases
ACTTTTTTTACAAAATTTATCAAAAACTCTTTAGAGCCTTTCTTAACTTTTCACTCATTTCATCAATATGTTCTTTTTGC
AAAATCAAAGGAGGTAAAAA

Product: protein-L-isoaspartate O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT [H]

Number of amino acids: Translated: 209; Mature: 209

Protein sequence:

>209_residues
MNAFEQKRCQNMAEEIAQKVFINEELFNAFCQIPREIFSPLKAHAYRLDALPLANSQWISSPLTVAKMTMALDFKNADSI
LEIGCGSGYQAAILSKVIRRVFTIERIENLAKKAAQTFRELELFNINVKFDDGQNGWKNYAPYDRILFSAYATQIPEILL
DQLSDDGILVAPILHNGKQFITRLRKNGTNLQKEILEECLFVPIVDGKE

Sequences:

>Translated_209_residues
MNAFEQKRCQNMAEEIAQKVFINEELFNAFCQIPREIFSPLKAHAYRLDALPLANSQWISSPLTVAKMTMALDFKNADSI
LEIGCGSGYQAAILSKVIRRVFTIERIENLAKKAAQTFRELELFNINVKFDDGQNGWKNYAPYDRILFSAYATQIPEILL
DQLSDDGILVAPILHNGKQFITRLRKNGTNLQKEILEECLFVPIVDGKE
>Mature_209_residues
MNAFEQKRCQNMAEEIAQKVFINEELFNAFCQIPREIFSPLKAHAYRLDALPLANSQWISSPLTVAKMTMALDFKNADSI
LEIGCGSGYQAAILSKVIRRVFTIERIENLAKKAAQTFRELELFNINVKFDDGQNGWKNYAPYDRILFSAYATQIPEILL
DQLSDDGILVAPILHNGKQFITRLRKNGTNLQKEILEECLFVPIVDGKE

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins [H]

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789100, Length=204, Percent_Identity=35.2941176470588, Blast_Score=143, Evalue=7e-36,
Organism=Caenorhabditis elegans, GI71983477, Length=205, Percent_Identity=24.8780487804878, Blast_Score=70, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI193207222, Length=205, Percent_Identity=25.8536585365854, Blast_Score=67, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000682 [H]

Pfam domain/function: PF01135 PCMT [H]

EC number: =2.1.1.77 [H]

Molecular weight: Translated: 23789; Mature: 23789

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: PS01279 PCMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAFEQKRCQNMAEEIAQKVFINEELFNAFCQIPREIFSPLKAHAYRLDALPLANSQWIS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHC
SPLTVAKMTMALDFKNADSILEIGCGSGYQAAILSKVIRRVFTIERIENLAKKAAQTFRE
CCHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LELFNINVKFDDGQNGWKNYAPYDRILFSAYATQIPEILLDQLSDDGILVAPILHNGKQF
HHHEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHCCHHH
ITRLRKNGTNLQKEILEECLFVPIVDGKE
HHHHHHCCCCHHHHHHHHHHEECCCCCCC
>Mature Secondary Structure
MNAFEQKRCQNMAEEIAQKVFINEELFNAFCQIPREIFSPLKAHAYRLDALPLANSQWIS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHC
SPLTVAKMTMALDFKNADSILEIGCGSGYQAAILSKVIRRVFTIERIENLAKKAAQTFRE
CCHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LELFNINVKFDDGQNGWKNYAPYDRILFSAYATQIPEILLDQLSDDGILVAPILHNGKQF
HHHEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHCCHHH
ITRLRKNGTNLQKEILEECLFVPIVDGKE
HHHHHHCCCCHHHHHHHHHHEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA