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The map label for this gene is dam [H]

Identifier: 57237231

GI number: 57237231

Start: 209239

End: 210054

Strand: Reverse

Name: dam [H]

Synonym: CJE0220

Alternate gene names: 57237231

Gene position: 210054-209239 (Counterclockwise)

Preceding gene: 57237232

Following gene: 57237229

Centisome position: 11.82

GC content: 27.33

Gene sequence:

>816_bases
ATGAAAAATAATACAGACAAATTTCTAAAAACTAGCACACTTACTAAACCTACAAGAACTACACTAAAAGCTCCATTTGC
TTGGGTGGGTGGTAAAAACTATTTAGCTAAAGAAATCATTGCTTTAATGCCTGAGCATAAAAGCTATATTGAAGTCTTTG
GAGGAGCTTTAAGTGTTTTTTATCAAAAAAGTGCTTCAAAAATAGAAGTCATTAACGACATTAACGACGAGCTTATTAAT
TTACATCTTTGTATAAGAAACAAACCCCAAAGCCTAGCAAATGTGCTTAATTCTATGATAATAAGTAGAAAAATCTTTCA
TATGCTTAAAAATAAAGAAATTAAACCAAGAAATGACTTAGAAAGGGCTGCTTTTTATTTTTATCTTATCAGTACTTCTT
TTGGTTCAAGCATGGGACAATTTGCTATGAATAAACAAAGAGCACCAAAGAGATTATGTAGGGATTTTAGCTTACATACA
AAAAGACTTAAAAATGCCAGTATTGAAAATAAAAGCTTTGAATATATTTTAAAAGAATATGATTACAATGAAGCTTTATT
TTATTTAGATCCACCTTATGTAGGAACTGAGAATTATTATAAAAACACAGGAGGTTTTGGGCTAAAAGAACATGAACTTT
TATGTAATTTGCTTAAAAATATCAAGGGTAAATTTATGCTTTCTTATAATGATTGTGAGCTTATAAGAAAGCTTTATAAA
GATTTTAATTTTAAGGAGTTGAAAGTAAGGTATTCTTTAAATAATAATGTTTTAAAAAGAAAAGAAAGTAAAGAGCTTTT
GATTATGAATTTTTAA

Upstream 100 bases:

>100_bases
AAGGAAGACTAAAGCCTTCCTTTTTCTAAAAACTAGCAAAAGCATTATAGCTTTGTTTTTAAAGCAAAGCTTTTTTAATT
TTATTTTGAAAGGAGTTTGT

Downstream 100 bases:

>100_bases
AAGCTAAGAGAGTAAAAATACTCTCTTTTATTTTAGCAAAGAGCTTAAAAACTTTAGAGCTTCTTTGCTAAAATCTTTAT
TGCATTCTTCTAGGTAATTT

Product: DNA adenine methylase

Products: NA

Alternate protein names: M.MboIA; Adenine-specific methyltransferase MboIA [H]

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MKNNTDKFLKTSTLTKPTRTTLKAPFAWVGGKNYLAKEIIALMPEHKSYIEVFGGALSVFYQKSASKIEVINDINDELIN
LHLCIRNKPQSLANVLNSMIISRKIFHMLKNKEIKPRNDLERAAFYFYLISTSFGSSMGQFAMNKQRAPKRLCRDFSLHT
KRLKNASIENKSFEYILKEYDYNEALFYLDPPYVGTENYYKNTGGFGLKEHELLCNLLKNIKGKFMLSYNDCELIRKLYK
DFNFKELKVRYSLNNNVLKRKESKELLIMNF

Sequences:

>Translated_271_residues
MKNNTDKFLKTSTLTKPTRTTLKAPFAWVGGKNYLAKEIIALMPEHKSYIEVFGGALSVFYQKSASKIEVINDINDELIN
LHLCIRNKPQSLANVLNSMIISRKIFHMLKNKEIKPRNDLERAAFYFYLISTSFGSSMGQFAMNKQRAPKRLCRDFSLHT
KRLKNASIENKSFEYILKEYDYNEALFYLDPPYVGTENYYKNTGGFGLKEHELLCNLLKNIKGKFMLSYNDCELIRKLYK
DFNFKELKVRYSLNNNVLKRKESKELLIMNF
>Mature_271_residues
MKNNTDKFLKTSTLTKPTRTTLKAPFAWVGGKNYLAKEIIALMPEHKSYIEVFGGALSVFYQKSASKIEVINDINDELIN
LHLCIRNKPQSLANVLNSMIISRKIFHMLKNKEIKPRNDLERAAFYFYLISTSFGSSMGQFAMNKQRAPKRLCRDFSLHT
KRLKNASIENKSFEYILKEYDYNEALFYLDPPYVGTENYYKNTGGFGLKEHELLCNLLKNIKGKFMLSYNDCELIRKLYK
DFNFKELKVRYSLNNNVLKRKESKELLIMNF

Specific function: This methylase recognizes the double-stranded sequence GATC, causes specific methylation on A-2 on both strands, and protects the DNA from cleavage by the MboI endonuclease [H]

COG id: COG0338

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789789, Length=260, Percent_Identity=23.4615384615385, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023095
- InterPro:   IPR012327 [H]

Pfam domain/function: PF02086 MethyltransfD12 [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 31703; Mature: 31703

Theoretical pI: Translated: 10.10; Mature: 10.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNNTDKFLKTSTLTKPTRTTLKAPFAWVGGKNYLAKEIIALMPEHKSYIEVFGGALSVF
CCCCHHHHHHHHCCCCCCHHHHHCCEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHH
YQKSASKIEVINDINDELINLHLCIRNKPQSLANVLNSMIISRKIFHMLKNKEIKPRNDL
HHHCCCHHHHHHHCCHHHEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
ERAAFYFYLISTSFGSSMGQFAMNKQRAPKRLCRDFSLHTKRLKNASIENKSFEYILKEY
HHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHC
DYNEALFYLDPPYVGTENYYKNTGGFGLKEHELLCNLLKNIKGKFMLSYNDCELIRKLYK
CCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHH
DFNFKELKVRYSLNNNVLKRKESKELLIMNF
CCCCEEEEEEEECCCCHHHCCCCCCEEEEEC
>Mature Secondary Structure
MKNNTDKFLKTSTLTKPTRTTLKAPFAWVGGKNYLAKEIIALMPEHKSYIEVFGGALSVF
CCCCHHHHHHHHCCCCCCHHHHHCCEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHH
YQKSASKIEVINDINDELINLHLCIRNKPQSLANVLNSMIISRKIFHMLKNKEIKPRNDL
HHHCCCHHHHHHHCCHHHEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
ERAAFYFYLISTSFGSSMGQFAMNKQRAPKRLCRDFSLHTKRLKNASIENKSFEYILKEY
HHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHC
DYNEALFYLDPPYVGTENYYKNTGGFGLKEHELLCNLLKNIKGKFMLSYNDCELIRKLYK
CCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHH
DFNFKELKVRYSLNNNVLKRKESKELLIMNF
CCCCEEEEEEEECCCCHHHCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8506128 [H]