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The map label for this gene is birA [C]

Identifier: 57237106

GI number: 57237106

Start: 106671

End: 107324

Strand: Direct

Name: birA [C]

Synonym: CJE0094

Alternate gene names: 57237106

Gene position: 106671-107324 (Clockwise)

Preceding gene: 57237105

Following gene: 57237107

Centisome position: 6.0

GC content: 28.44

Gene sequence:

>654_bases
ATGGAAAAAGGCTTAAAAGTGAAGATAGTTTGTGTTGAAAGTATAGACTCAACTCATCTTTTTTTATGCGAGCAAATACG
CAATGGAAAGATTGATGAAAATTTTGCTATTTATGCTTTAGAACAAACAAATGGAGTTGGAAGTAGGGAAAATTCTTGGC
AAAGCTCTAAAGGAAATTTACATCTTTCTTTTTGTATTAAAGAAGAAGATTTACCAAAAGATTTACCTTTAGCTTCTGTG
AGTATTTATTTTGCTTATTTACTTAAAGAAGTTTTACAAGAAAAAGGTTCTAAAATTTGGCTTAAATGGCCTAACGATCT
ATATTTAGATGATAAAAAAGCTGGAGGAGTTATAAGTGCTAAAATTTCAAATTTTATCATAGGCGGTATGGGTTTAAATC
TGAAATTTTCACCCCAAAATACAGCTTTATGCGATATAGAAATTTCGCTTAAAGATTTAGTGAGTGAGTTTTTGCAAAAA
GTAGAAAAAAAAATTTTATGGAAGAATATTTTTAGCAAGTATATGTTAGAATTTGAAAAATCAAGAAAATTTAGCGTCCA
TCATGAAGGTAAAGTTTTTTCACTTGAAAATTCTTTTTTGTATGAAGATGGTTCAATTTTACTAGGTGATAAAAGGGTGT
ATAGTTTAAGATGA

Upstream 100 bases:

>100_bases
GAAAAAGAAAGTTTTTTGCTTGCTTGCAAACAAGGAGTTTTGCGTATTAAAAAACTTCAAGAAAGTGGAAAAAAAGCTCT
TGATGGCAGGACTTATTTAA

Downstream 100 bases:

>100_bases
GCGAAATTATTACAATAGCAAACCAAAAAGGTGGGGTAGGAAAGACTACAACAGCAGTAAATTTAGCCGCTTCTTTAGCT
GTGGCTGAAAAAAAAGTACT

Product: biotin--protein ligase

Products: AMP; diphosphate; [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]

Alternate protein names: Biotin/Acetyl-CoA-Carboxylase Ligase; Biotin-[Acetyl-CoA-Carboxylase] Ligase; Biotin Operon Repressor; Biotin-[Acetyl-CoA-Carboxylase] Synthetase; Biotin-Acetyl-CoA-Carboxylase Ligase; Biotin-(Acetyl-CoA Carboxylase) Ligase; Biotin-(Acetyl-CoA-Carboxylase) Ligase; Protein Ligase

Number of amino acids: Translated: 217; Mature: 217

Protein sequence:

>217_residues
MEKGLKVKIVCVESIDSTHLFLCEQIRNGKIDENFAIYALEQTNGVGSRENSWQSSKGNLHLSFCIKEEDLPKDLPLASV
SIYFAYLLKEVLQEKGSKIWLKWPNDLYLDDKKAGGVISAKISNFIIGGMGLNLKFSPQNTALCDIEISLKDLVSEFLQK
VEKKILWKNIFSKYMLEFEKSRKFSVHHEGKVFSLENSFLYEDGSILLGDKRVYSLR

Sequences:

>Translated_217_residues
MEKGLKVKIVCVESIDSTHLFLCEQIRNGKIDENFAIYALEQTNGVGSRENSWQSSKGNLHLSFCIKEEDLPKDLPLASV
SIYFAYLLKEVLQEKGSKIWLKWPNDLYLDDKKAGGVISAKISNFIIGGMGLNLKFSPQNTALCDIEISLKDLVSEFLQK
VEKKILWKNIFSKYMLEFEKSRKFSVHHEGKVFSLENSFLYEDGSILLGDKRVYSLR
>Mature_217_residues
MEKGLKVKIVCVESIDSTHLFLCEQIRNGKIDENFAIYALEQTNGVGSRENSWQSSKGNLHLSFCIKEEDLPKDLPLASV
SIYFAYLLKEVLQEKGSKIWLKWPNDLYLDDKKAGGVISAKISNFIIGGMGLNLKFSPQNTALCDIEISLKDLVSEFLQK
VEKKILWKNIFSKYMLEFEKSRKFSVHHEGKVFSLENSFLYEDGSILLGDKRVYSLR

Specific function: Bira Acts Both As A Biotin-Operon Repressor And As The Enzyme That Synthesizes The Corepressor, Acetyl-CoA:Carbon- Dioxide Ligase. This Protein Also Activates Biotin To Form Biotinyl-5'-Adenylate And Transfers The Biotin Moiety To Biotin-Accepting Protei

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 6.3.4.15

Molecular weight: Translated: 24811; Mature: 24811

Theoretical pI: Translated: 7.95; Mature: 7.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKGLKVKIVCVESIDSTHLFLCEQIRNGKIDENFAIYALEQTNGVGSRENSWQSSKGNL
CCCCCEEEEEEEECCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCE
HLSFCIKEEDLPKDLPLASVSIYFAYLLKEVLQEKGSKIWLKWPNDLYLDDKKAGGVISA
EEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEECCCCCCCEEEE
KISNFIIGGMGLNLKFSPQNTALCDIEISLKDLVSEFLQKVEKKILWKNIFSKYMLEFEK
ECCCEEEECCCEEEEECCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SRKFSVHHEGKVFSLENSFLYEDGSILLGDKRVYSLR
CCCEEEEECCEEEEEECCEEEECCCEEEECCEEEECC
>Mature Secondary Structure
MEKGLKVKIVCVESIDSTHLFLCEQIRNGKIDENFAIYALEQTNGVGSRENSWQSSKGNL
CCCCCEEEEEEEECCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCE
HLSFCIKEEDLPKDLPLASVSIYFAYLLKEVLQEKGSKIWLKWPNDLYLDDKKAGGVISA
EEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEECCCCCCCEEEE
KISNFIIGGMGLNLKFSPQNTALCDIEISLKDLVSEFLQKVEKKILWKNIFSKYMLEFEK
ECCCEEEECCCEEEEECCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SRKFSVHHEGKVFSLENSFLYEDGSILLGDKRVYSLR
CCCEEEEECCEEEEEECCEEEECCCEEEECCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; biotin; apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)]

Specific reaction: ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] = AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]

General reaction: Amination; Carboxylic acid formation (carboxamide formation [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA