Click here to switch to the map view.

The map label for this gene is pleD [H]

Identifier: 57236956

GI number: 57236956

Start: 681823

End: 683067

Strand: Direct

Name: pleD [H]

Synonym: CJE0746

Alternate gene names: 57236956

Gene position: 681823-683067 (Clockwise)

Preceding gene: 57236955

Following gene: 57236957

Centisome position: 38.35

GC content: 29.56

Gene sequence:

>1245_bases
ATGAATAAGAAAATTTTAATTATTGATGACAATAAAATGCTCGGCAAACTTTTGGCTAAAAAAATTCAAATGACTTTAGA
CTATGAGGTAGATATTGCTTTTGGTTTTGCTGAAGCTAAGGAGTTAATGAATAATGATTATTTTTTAGCTTTTGTGGATT
TATGCTTGCCAGATGCCCCAAATGGTGAAGTTGTGGATTATGTTATAGAAAAGAAAATTCCTGCTATTGTTCTTACGGCT
AGTGGTGATAAGGCTACTAAAGAAAAATTTATGGATAAAGATATATTAGATTATATTTTTAAAGAAAGTGAAACTTGCAT
TGATGAGATTATTAGTTCTATTGTAAAACTTAATCAATATGCAAAAACTAAAGTGATTTTAGCTATGAGCAAGCTACCTG
AACGTAATGAAATTAAAAAAATACTTACTCAAAGACAATTTAATGTTTTAGCTGCAGCTCATGGTGAAGAAGCAATGAGT
TATTTAAATGATAATAATGATGTTAAGCTTATTATAGCAGATGTCAATATGCCAGTAATTAGCGGTTTTGAGCTTTTGAC
TCAAGTAAGAGAACGTTTTAGTGACGATGAATTAGGTGTGATTCTTTTGGGGGATCATAATGATTCATTTGAAGCGAATT
CTTTTAAAAATGGAGTTAATGAGTATTTGTTTAAGCCTTTAAGCAAAGAAAGTTTTAATTGTCGCTTAGATCGTTGTTTA
TCTTACATGGATGATAAAAAATTTTTAAGCACTTATAATGTTTTAGATCCTGTATCTGGGGTTAAAAATTATAATGCTTT
AATCGATGGAATAGATGATTATTTTAACGAAATAGCAACAAAAGACGAGGAATTTGCTTTTGCTTTCTTAGATATAGATA
ATCTTCAAATGATTAACGATGAATACGGCCGTGAAGTTGGTGATGAAGTAATTAAAATCTGCGCCAATGAAATAGTTAAT
GAAACTAAAGGAAGAGATCTTGTAGGTAGATATAGTGCAGAAAAAATTTGTATTTTACTTAAAAATATATCTCAAGAAAG
AGCGATTAAAATCTTTTCTCGTATACGAGTAAATATAAAAAAGGCTGGTGTTTTGGTTAATTTAGATGAAGTCTTTTTTA
CAGCTTCTATAGGCGTGGTTTTTGGAAAAAGTGGAGATAAGATTGATTCTTTGGTTGATAAAGCAAGTAAAATATTGTCT
CAAGCAAAAGATAATGGCAAGGATAGAGTTGAAGTATGTTCTTAA

Upstream 100 bases:

>100_bases
TTTTTTATTTTGCTATGTTAAAATATATAACTATAAAAATTTCCTTTCTTTTGAAACAAATATAATAAAAAATTTTAGAT
ATAAAGGTATTTTACACACT

Downstream 100 bases:

>100_bases
ATCTTGATTTTCAAGATGGTTTAAGGATTGTTGATACACATTGCCATCTAGACAGTGAAGCATTTAAAGATGATTTAGAT
GAAACTTTAAATCGTGCTTT

Product: response regulator/GGDEF domain-containing protein

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MNKKILIIDDNKMLGKLLAKKIQMTLDYEVDIAFGFAEAKELMNNDYFLAFVDLCLPDAPNGEVVDYVIEKKIPAIVLTA
SGDKATKEKFMDKDILDYIFKESETCIDEIISSIVKLNQYAKTKVILAMSKLPERNEIKKILTQRQFNVLAAAHGEEAMS
YLNDNNDVKLIIADVNMPVISGFELLTQVRERFSDDELGVILLGDHNDSFEANSFKNGVNEYLFKPLSKESFNCRLDRCL
SYMDDKKFLSTYNVLDPVSGVKNYNALIDGIDDYFNEIATKDEEFAFAFLDIDNLQMINDEYGREVGDEVIKICANEIVN
ETKGRDLVGRYSAEKICILLKNISQERAIKIFSRIRVNIKKAGVLVNLDEVFFTASIGVVFGKSGDKIDSLVDKASKILS
QAKDNGKDRVEVCS

Sequences:

>Translated_414_residues
MNKKILIIDDNKMLGKLLAKKIQMTLDYEVDIAFGFAEAKELMNNDYFLAFVDLCLPDAPNGEVVDYVIEKKIPAIVLTA
SGDKATKEKFMDKDILDYIFKESETCIDEIISSIVKLNQYAKTKVILAMSKLPERNEIKKILTQRQFNVLAAAHGEEAMS
YLNDNNDVKLIIADVNMPVISGFELLTQVRERFSDDELGVILLGDHNDSFEANSFKNGVNEYLFKPLSKESFNCRLDRCL
SYMDDKKFLSTYNVLDPVSGVKNYNALIDGIDDYFNEIATKDEEFAFAFLDIDNLQMINDEYGREVGDEVIKICANEIVN
ETKGRDLVGRYSAEKICILLKNISQERAIKIFSRIRVNIKKAGVLVNLDEVFFTASIGVVFGKSGDKIDSLVDKASKILS
QAKDNGKDRVEVCS
>Mature_414_residues
MNKKILIIDDNKMLGKLLAKKIQMTLDYEVDIAFGFAEAKELMNNDYFLAFVDLCLPDAPNGEVVDYVIEKKIPAIVLTA
SGDKATKEKFMDKDILDYIFKESETCIDEIISSIVKLNQYAKTKVILAMSKLPERNEIKKILTQRQFNVLAAAHGEEAMS
YLNDNNDVKLIIADVNMPVISGFELLTQVRERFSDDELGVILLGDHNDSFEANSFKNGVNEYLFKPLSKESFNCRLDRCL
SYMDDKKFLSTYNVLDPVSGVKNYNALIDGIDDYFNEIATKDEEFAFAFLDIDNLQMINDEYGREVGDEVIKICANEIVN
ETKGRDLVGRYSAEKICILLKNISQERAIKIFSRIRVNIKKAGVLVNLDEVFFTASIGVVFGKSGDKIDSLVDKASKILS
QAKDNGKDRVEVCS

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI87081881, Length=128, Percent_Identity=31.25, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI87082007, Length=161, Percent_Identity=27.3291925465839, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1786584, Length=172, Percent_Identity=23.8372093023256, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI1787262, Length=171, Percent_Identity=29.8245614035088, Blast_Score=65, Evalue=9e-12,
Organism=Escherichia coli, GI1787541, Length=157, Percent_Identity=24.8407643312102, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 46709; Mature: 46709

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKKILIIDDNKMLGKLLAKKIQMTLDYEVDIAFGFAEAKELMNNDYFLAFVDLCLPDAP
CCCEEEEEECCHHHHHHHHHHHHHEEEEEEEEEECHHHHHHHHCCCEEEEEHHHHCCCCC
NGEVVDYVIEKKIPAIVLTASGDKATKEKFMDKDILDYIFKESETCIDEIISSIVKLNQY
CCCHHHHHHHCCCCEEEEECCCCHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHH
AKTKVILAMSKLPERNEIKKILTQRQFNVLAAAHGEEAMSYLNDNNDVKLIIADVNMPVI
HHHHHEEHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCEEEEEEECCCCHH
SGFELLTQVRERFSDDELGVILLGDHNDSFEANSFKNGVNEYLFKPLSKESFNCRLDRCL
HHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHH
SYMDDKKFLSTYNVLDPVSGVKNYNALIDGIDDYFNEIATKDEEFAFAFLDIDNLQMIND
HHHCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHH
EYGREVGDEVIKICANEIVNETKGRDLVGRYSAEKICILLKNISQERAIKIFSRIRVNIK
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHEEE
KAGVLVNLDEVFFTASIGVVFGKSGDKIDSLVDKASKILSQAKDNGKDRVEVCS
CCCEEEEHHHHHHHEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCEEECC
>Mature Secondary Structure
MNKKILIIDDNKMLGKLLAKKIQMTLDYEVDIAFGFAEAKELMNNDYFLAFVDLCLPDAP
CCCEEEEEECCHHHHHHHHHHHHHEEEEEEEEEECHHHHHHHHCCCEEEEEHHHHCCCCC
NGEVVDYVIEKKIPAIVLTASGDKATKEKFMDKDILDYIFKESETCIDEIISSIVKLNQY
CCCHHHHHHHCCCCEEEEECCCCHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHH
AKTKVILAMSKLPERNEIKKILTQRQFNVLAAAHGEEAMSYLNDNNDVKLIIADVNMPVI
HHHHHEEHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCEEEEEEECCCCHH
SGFELLTQVRERFSDDELGVILLGDHNDSFEANSFKNGVNEYLFKPLSKESFNCRLDRCL
HHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHH
SYMDDKKFLSTYNVLDPVSGVKNYNALIDGIDDYFNEIATKDEEFAFAFLDIDNLQMIND
HHHCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHH
EYGREVGDEVIKICANEIVNETKGRDLVGRYSAEKICILLKNISQERAIKIFSRIRVNIK
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHEEE
KAGVLVNLDEVFFTASIGVVFGKSGDKIDSLVDKASKILSQAKDNGKDRVEVCS
CCCEEEEHHHHHHHEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA