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The map label for this gene is aspS [H]

Identifier: 57236953

GI number: 57236953

Start: 677492

End: 679243

Strand: Reverse

Name: aspS [H]

Synonym: CJE0743

Alternate gene names: 57236953

Gene position: 679243-677492 (Counterclockwise)

Preceding gene: 57236966

Following gene: 57236952

Centisome position: 38.21

GC content: 35.33

Gene sequence:

>1752_bases
TTGCGAAGTCATTATAATACAGACTTGGGAATTTCCCATGTTGGTCAAAGCGTTAAACTTTGCGGTTGGGTAAATAGCTA
CCGCGATCATGGTGGAGTTATTTTTATAGATTTAAGAGATAGAAGTGGTATTATCCAACTTGTTTGTGATCCAAATGATA
GCAAAGAAGCTCATGAAATCGCTTCCAATGCAAGAAATGAATTTGTACTTATTGCAGAAGGCACTATTCGTCCGCGTGGC
GAAGGACTTATAAATCCTAAATTAAAAACAGGAGAAATCGAAGTAGTTGTTTCTAAACTTACCATAGAAAATGAAAGTGC
CGTACCTCCTTTTGCTATCGCTGATGAAAGTGTAAATGAAGAATTACGCTTAAAATATCGCTTTTTAGATTTAAGAAATC
CTAAACTTTATGAAAATTTTGCCTTGCGTTCTAAAGCTTGTATAGCAGCTAGAAATTCTTTGGCAAATATGGGATTTTTA
GAAGTTGAAACCCCTATTCTTACCAAAGCTACTCCCGAAGGCGCAAGAGATTATCTCGTGCCTTCTCGTGTGCATCAGGG
TGAATTTTATGCTCTTCCACAAAGCCCTCAGCTTTTCAAACAACTTTTAATGTGTAGCGGTTTTGATCGTTATTTTCAAA
TCGCAAAATGTTTTAGAGATGAAGATTTAAGAGCTGATCGCCAACCCGAATTCACACAAATTGATATTGAAATGAGTTTT
TGCGAACAAAAAGATGTAATCAATGTAGCTGAAACTTTTTTAAAAGATATTTTCAAAGCTTGCGGCAAAGAAATTCAAAC
CCCTTTTAGACAAATGCAATACAAAGACGCTATGGAAAATTATGGCTCTGATAAGCCGGATTTAAGATTTAATCTTAAAT
TTATTGATGTGATTGATATTTTCGCTAAGTCAAATAATGAAATTTTTGCAAATATTGCCAAAGATACAAAGAAAAATCGC
ATCAAAGCCATACGCGTTCCAAAAGGCGATACTATTTTTTCAAAAAGACAAATGCAAAGATTTGAAGAATTTGTGCGTAA
ATTTGGCGCCCAAGGCTTAGCTTTCATACAAGTTAAAGAAGATGGGCTTAAAGGTCCACTTTGCAAATTCTTTAGCGAAG
AAGATTTAAATGAGCTAAGCAAACGCTGTGAGCTTGAAGTAGGAGATGTGGTATTTTTCGGTGCAGGAGCTAAAAAAACC
GTGCTTGATTATATGGGAAGATTTAGAATTTTCTTAGCCAACGAGTTAAAACTTATAGATCCAAATGCCTTGGAATTCTT
ATGGGTTGTTGATTTTCCTATGTTTGAGCAAAATGATGACGGAAGTTATTCTGCGATGCACCATCCTTTTACCATGCCAA
AAAATATTGATGAGACTGATTTAGAAGAAATTTCATCTATTGCTTATGATGTAGTGTTAAATGGCGTTGAGCTAGGTGGA
GGTAGTATAAGAATCCATAAAAATGATATTCAACAAAAAGTATTTAAACTTTTAAATATCGATGAAGAACAACAAAAAGA
AAAATTTGGCTTCTTGCTTGATGCTTTAAGTTTTGGTGCTCCACCACATGGAGGTATCGCTATAGGACTTGATAGACTCA
TCATGCTAGTTACAGGAGCAAACAACATAAGAGAGGTTATAGCCTTTCCAAAAACCCAAAGAGCGCAATGCTTAATGACA
GATGCACCATCGCCTGCTAGCAATGAAGCAATGAGAGAATTAGGAATCAAATTAAGGGAGAATATAAAATGA

Upstream 100 bases:

>100_bases
TATTTTGCATAAAAAATTATATCAAATCTTAGCAAAGCAAAAGAATAAAAATACTATAATTTTGCTCATTATCTTAAAAA
ATCTTTTTCAAGGAATAAAT

Downstream 100 bases:

>100_bases
AAGAATTGTTTTTAATCATAGGTGCACCAGGTAGTGGAAAAACTACCGATGCTAGTTTAATCGCACAAGCAGATGCGACA
AATATTACACACTATTCAAC

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS [H]

Number of amino acids: Translated: 583; Mature: 583

Protein sequence:

>583_residues
MRSHYNTDLGISHVGQSVKLCGWVNSYRDHGGVIFIDLRDRSGIIQLVCDPNDSKEAHEIASNARNEFVLIAEGTIRPRG
EGLINPKLKTGEIEVVVSKLTIENESAVPPFAIADESVNEELRLKYRFLDLRNPKLYENFALRSKACIAARNSLANMGFL
EVETPILTKATPEGARDYLVPSRVHQGEFYALPQSPQLFKQLLMCSGFDRYFQIAKCFRDEDLRADRQPEFTQIDIEMSF
CEQKDVINVAETFLKDIFKACGKEIQTPFRQMQYKDAMENYGSDKPDLRFNLKFIDVIDIFAKSNNEIFANIAKDTKKNR
IKAIRVPKGDTIFSKRQMQRFEEFVRKFGAQGLAFIQVKEDGLKGPLCKFFSEEDLNELSKRCELEVGDVVFFGAGAKKT
VLDYMGRFRIFLANELKLIDPNALEFLWVVDFPMFEQNDDGSYSAMHHPFTMPKNIDETDLEEISSIAYDVVLNGVELGG
GSIRIHKNDIQQKVFKLLNIDEEQQKEKFGFLLDALSFGAPPHGGIAIGLDRLIMLVTGANNIREVIAFPKTQRAQCLMT
DAPSPASNEAMRELGIKLRENIK

Sequences:

>Translated_583_residues
MRSHYNTDLGISHVGQSVKLCGWVNSYRDHGGVIFIDLRDRSGIIQLVCDPNDSKEAHEIASNARNEFVLIAEGTIRPRG
EGLINPKLKTGEIEVVVSKLTIENESAVPPFAIADESVNEELRLKYRFLDLRNPKLYENFALRSKACIAARNSLANMGFL
EVETPILTKATPEGARDYLVPSRVHQGEFYALPQSPQLFKQLLMCSGFDRYFQIAKCFRDEDLRADRQPEFTQIDIEMSF
CEQKDVINVAETFLKDIFKACGKEIQTPFRQMQYKDAMENYGSDKPDLRFNLKFIDVIDIFAKSNNEIFANIAKDTKKNR
IKAIRVPKGDTIFSKRQMQRFEEFVRKFGAQGLAFIQVKEDGLKGPLCKFFSEEDLNELSKRCELEVGDVVFFGAGAKKT
VLDYMGRFRIFLANELKLIDPNALEFLWVVDFPMFEQNDDGSYSAMHHPFTMPKNIDETDLEEISSIAYDVVLNGVELGG
GSIRIHKNDIQQKVFKLLNIDEEQQKEKFGFLLDALSFGAPPHGGIAIGLDRLIMLVTGANNIREVIAFPKTQRAQCLMT
DAPSPASNEAMRELGIKLRENIK
>Mature_583_residues
MRSHYNTDLGISHVGQSVKLCGWVNSYRDHGGVIFIDLRDRSGIIQLVCDPNDSKEAHEIASNARNEFVLIAEGTIRPRG
EGLINPKLKTGEIEVVVSKLTIENESAVPPFAIADESVNEELRLKYRFLDLRNPKLYENFALRSKACIAARNSLANMGFL
EVETPILTKATPEGARDYLVPSRVHQGEFYALPQSPQLFKQLLMCSGFDRYFQIAKCFRDEDLRADRQPEFTQIDIEMSF
CEQKDVINVAETFLKDIFKACGKEIQTPFRQMQYKDAMENYGSDKPDLRFNLKFIDVIDIFAKSNNEIFANIAKDTKKNR
IKAIRVPKGDTIFSKRQMQRFEEFVRKFGAQGLAFIQVKEDGLKGPLCKFFSEEDLNELSKRCELEVGDVVFFGAGAKKT
VLDYMGRFRIFLANELKLIDPNALEFLWVVDFPMFEQNDDGSYSAMHHPFTMPKNIDETDLEEISSIAYDVVLNGVELGG
GSIRIHKNDIQQKVFKLLNIDEEQQKEKFGFLLDALSFGAPPHGGIAIGLDRLIMLVTGANNIREVIAFPKTQRAQCLMT
DAPSPASNEAMRELGIKLRENIK

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI40789249, Length=594, Percent_Identity=39.8989898989899, Blast_Score=409, Evalue=1e-114,
Organism=Homo sapiens, GI45439306, Length=318, Percent_Identity=28.6163522012579, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI194272210, Length=256, Percent_Identity=26.953125, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI5031815, Length=256, Percent_Identity=26.953125, Blast_Score=86, Evalue=1e-16,
Organism=Escherichia coli, GI1788173, Length=587, Percent_Identity=51.4480408858603, Blast_Score=598, Evalue=1e-172,
Organism=Escherichia coli, GI1789256, Length=280, Percent_Identity=26.7857142857143, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI1790571, Length=291, Percent_Identity=25.7731958762887, Blast_Score=92, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI32566633, Length=606, Percent_Identity=36.3036303630363, Blast_Score=332, Evalue=3e-91,
Organism=Caenorhabditis elegans, GI71994340, Length=256, Percent_Identity=26.953125, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17535925, Length=256, Percent_Identity=26.953125, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17535927, Length=256, Percent_Identity=26.953125, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17551876, Length=334, Percent_Identity=25.748502994012, Blast_Score=86, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6325153, Length=648, Percent_Identity=32.4074074074074, Blast_Score=262, Evalue=1e-70,
Organism=Saccharomyces cerevisiae, GI6323011, Length=333, Percent_Identity=26.4264264264264, Blast_Score=85, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6321807, Length=289, Percent_Identity=27.681660899654, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6320242, Length=257, Percent_Identity=23.7354085603113, Blast_Score=67, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24584738, Length=598, Percent_Identity=39.1304347826087, Blast_Score=390, Evalue=1e-108,
Organism=Drosophila melanogaster, GI17136276, Length=279, Percent_Identity=27.9569892473118, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24640849, Length=271, Percent_Identity=27.6752767527675, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24640851, Length=271, Percent_Identity=27.6752767527675, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI19921528, Length=290, Percent_Identity=26.8965517241379, Blast_Score=73, Evalue=7e-13,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.12 [H]

Molecular weight: Translated: 66149; Mature: 66149

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSHYNTDLGISHVGQSVKLCGWVNSYRDHGGVIFIDLRDRSGIIQLVCDPNDSKEAHEI
CCCCCCCCCCHHHCCCCCEEHHHHHHHCCCCCEEEEEEECCCCEEEEEECCCCCHHHHHH
ASNARNEFVLIAEGTIRPRGEGLINPKLKTGEIEVVVSKLTIENESAVPPFAIADESVNE
HHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCEEECCCCCCH
ELRLKYRFLDLRNPKLYENFALRSKACIAARNSLANMGFLEVETPILTKATPEGARDYLV
HHEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEECCCCCCCCCCCC
PSRVHQGEFYALPQSPQLFKQLLMCSGFDRYFQIAKCFRDEDLRADRQPEFTQIDIEMSF
CCCCCCCCEEECCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEHH
CEQKDVINVAETFLKDIFKACGKEIQTPFRQMQYKDAMENYGSDKPDLRFNLKFIDVIDI
CCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEEEH
FAKSNNEIFANIAKDTKKNRIKAIRVPKGDTIFSKRQMQRFEEFVRKFGAQGLAFIQVKE
HHCCCCCEEEEEHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEC
DGLKGPLCKFFSEEDLNELSKRCELEVGDVVFFGAGAKKTVLDYMGRFRIFLANELKLID
CCCCCHHHHHCCCCHHHHHHHHCCCEECCEEEECCCCHHHHHHHHHHEEEEEECCEEEEC
PNALEFLWVVDFPMFEQNDDGSYSAMHHPFTMPKNIDETDLEEISSIAYDVVLNGVELGG
CCCEEEEEEECCCCCCCCCCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEECC
GSIRIHKNDIQQKVFKLLNIDEEQQKEKFGFLLDALSFGAPPHGGIAIGLDRLIMLVTGA
CEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCEEECHHEEEEEEECC
NNIREVIAFPKTQRAQCLMTDAPSPASNEAMRELGIKLRENIK
CHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHCHHHHHCCC
>Mature Secondary Structure
MRSHYNTDLGISHVGQSVKLCGWVNSYRDHGGVIFIDLRDRSGIIQLVCDPNDSKEAHEI
CCCCCCCCCCHHHCCCCCEEHHHHHHHCCCCCEEEEEEECCCCEEEEEECCCCCHHHHHH
ASNARNEFVLIAEGTIRPRGEGLINPKLKTGEIEVVVSKLTIENESAVPPFAIADESVNE
HHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCEEECCCCCCH
ELRLKYRFLDLRNPKLYENFALRSKACIAARNSLANMGFLEVETPILTKATPEGARDYLV
HHEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEECCCCCCCCCCCC
PSRVHQGEFYALPQSPQLFKQLLMCSGFDRYFQIAKCFRDEDLRADRQPEFTQIDIEMSF
CCCCCCCCEEECCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEHH
CEQKDVINVAETFLKDIFKACGKEIQTPFRQMQYKDAMENYGSDKPDLRFNLKFIDVIDI
CCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEEEH
FAKSNNEIFANIAKDTKKNRIKAIRVPKGDTIFSKRQMQRFEEFVRKFGAQGLAFIQVKE
HHCCCCCEEEEEHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEC
DGLKGPLCKFFSEEDLNELSKRCELEVGDVVFFGAGAKKTVLDYMGRFRIFLANELKLID
CCCCCHHHHHCCCCHHHHHHHHCCCEECCEEEECCCCHHHHHHHHHHEEEEEECCEEEEC
PNALEFLWVVDFPMFEQNDDGSYSAMHHPFTMPKNIDETDLEEISSIAYDVVLNGVELGG
CCCEEEEEEECCCCCCCCCCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEECC
GSIRIHKNDIQQKVFKLLNIDEEQQKEKFGFLLDALSFGAPPHGGIAIGLDRLIMLVTGA
CEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCEEECHHEEEEEEECC
NNIREVIAFPKTQRAQCLMTDAPSPASNEAMRELGIKLRENIK
CHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA