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The map label for this gene is rlmN [H]

Identifier: 57236899

GI number: 57236899

Start: 1760947

End: 1762017

Strand: Direct

Name: rlmN [H]

Synonym: CJE1882

Alternate gene names: 57236899

Gene position: 1760947-1762017 (Clockwise)

Preceding gene: 57236898

Following gene: 57236900

Centisome position: 99.05

GC content: 34.08

Gene sequence:

>1071_bases
TTGAAAGAGCTTGTTAATATTTTAGATTTTTTACCTGAAGAATTAGGAGAAAAAATCAAACCGATGTTTCGCGTGAAACA
AATTTATCAATGGATTTATCAAAAATACGCCAATAATTTTTCCGATATGTCAAGTTTACCAAAAGATCTACGTTTAGAAC
TTGCGCAAAATTTTCATTTCTCTCCTGTAAAATGTGTTAAAAATGAGCAAAGTAAAGATGGAAGTATAAAATATCTTTTT
GAACTCGTTGATGGTTTAAGGATAGAAAGTGTGCTTTTGCCTATGAAAGAAGAAAAAATAGACGCAGAAGGCAAAAGAAT
CTCTCATGCGAGATATACTATCTGTGTTTCTTCTCAAGTGGGTTGTAAAAGTGGATGTAGTTTTTGTCTTACTGCCAAGG
GTGGCTTGAAAAGAAATTTAAGTGCAGGGGAGATAGTCGGACAAATTTTATGGATCAAAAAACAAAACAATATCCCTTAT
GAACGCCGTATAAATATAGTATATATGGGTATGGGCGAGCCTTTGGATAATCTTAAAAATGTTTCTAAAGCGGTAAAAAT
TTTAGCACAAAATGAGGGGCTTGCCATAAGTCCCCGCCGTCAAACCATCAGCACTAGTGGTTTAGCAAAACAAATCAAAG
AATTAGGTCAAATGAACTTAGGTGTTTTACTTGCTATATCATTGCACGCGGTTAATGATGAGCTTCGAACCGAACTCATG
CCTATCAATAAAGCTTATAATATCGCTGCGATTATGGATGCGGTGCGTGAATTTCCTATAGATCAAAGAAAAAGAGTGAT
GTTTGAATATCTTTTAATTGATGGTATCAATGATAAGCTTGAACACGCTAAAGAATTAGTCAAGCTTTTAAATGGTATTA
AAGCCAAAGTAAATTTAATACTCTTTAATCCACATGAAGGAGGTTTATATAAACGCCCTAGTTTAGAAAACGCGATTAAA
TTTCAAGATTTACTCAGCAATAAAGGTGTAACTTGTACTATAAGAGAAAGTAAAGGGCTTGATATCTCAGCTGCTTGTGG
ACAGCTTAAAGAAAGGGCAAAAGAACAATGA

Upstream 100 bases:

>100_bases
ACGAAGATTTTATAAAAAATCATCAAAAGGCAAAAGAAAAATTAGAAGAATACTTAAAAAAATATCATTATATTTAAAAA
TTCAAAAAAGGAAAATAATT

Downstream 100 bases:

>100_bases
CAATGCTAGATATTTTTGAAATCATTTTTATAACAGCTGTTGTAATTATAGGTTTTGGTGGAATTGTTTTTGTAGTAACA
AAAGAAAAAAAATAATTATT

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase [H]

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MKELVNILDFLPEELGEKIKPMFRVKQIYQWIYQKYANNFSDMSSLPKDLRLELAQNFHFSPVKCVKNEQSKDGSIKYLF
ELVDGLRIESVLLPMKEEKIDAEGKRISHARYTICVSSQVGCKSGCSFCLTAKGGLKRNLSAGEIVGQILWIKKQNNIPY
ERRINIVYMGMGEPLDNLKNVSKAVKILAQNEGLAISPRRQTISTSGLAKQIKELGQMNLGVLLAISLHAVNDELRTELM
PINKAYNIAAIMDAVREFPIDQRKRVMFEYLLIDGINDKLEHAKELVKLLNGIKAKVNLILFNPHEGGLYKRPSLENAIK
FQDLLSNKGVTCTIRESKGLDISAACGQLKERAKEQ

Sequences:

>Translated_356_residues
MKELVNILDFLPEELGEKIKPMFRVKQIYQWIYQKYANNFSDMSSLPKDLRLELAQNFHFSPVKCVKNEQSKDGSIKYLF
ELVDGLRIESVLLPMKEEKIDAEGKRISHARYTICVSSQVGCKSGCSFCLTAKGGLKRNLSAGEIVGQILWIKKQNNIPY
ERRINIVYMGMGEPLDNLKNVSKAVKILAQNEGLAISPRRQTISTSGLAKQIKELGQMNLGVLLAISLHAVNDELRTELM
PINKAYNIAAIMDAVREFPIDQRKRVMFEYLLIDGINDKLEHAKELVKLLNGIKAKVNLILFNPHEGGLYKRPSLENAIK
FQDLLSNKGVTCTIRESKGLDISAACGQLKERAKEQ
>Mature_356_residues
MKELVNILDFLPEELGEKIKPMFRVKQIYQWIYQKYANNFSDMSSLPKDLRLELAQNFHFSPVKCVKNEQSKDGSIKYLF
ELVDGLRIESVLLPMKEEKIDAEGKRISHARYTICVSSQVGCKSGCSFCLTAKGGLKRNLSAGEIVGQILWIKKQNNIPY
ERRINIVYMGMGEPLDNLKNVSKAVKILAQNEGLAISPRRQTISTSGLAKQIKELGQMNLGVLLAISLHAVNDELRTELM
PINKAYNIAAIMDAVREFPIDQRKRVMFEYLLIDGINDKLEHAKELVKLLNGIKAKVNLILFNPHEGGLYKRPSLENAIK
FQDLLSNKGVTCTIRESKGLDISAACGQLKERAKEQ

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA [H]

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family [H]

Homologues:

Organism=Escherichia coli, GI1788865, Length=357, Percent_Identity=39.7759103641457, Blast_Score=246, Evalue=1e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.1.1.192 [H]

Molecular weight: Translated: 40211; Mature: 40211

Theoretical pI: Translated: 9.61; Mature: 9.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKELVNILDFLPEELGEKIKPMFRVKQIYQWIYQKYANNFSDMSSLPKDLRLELAQNFHF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHCCCC
SPVKCVKNEQSKDGSIKYLFELVDGLRIESVLLPMKEEKIDAEGKRISHARYTICVSSQV
CCHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHCCCHHHHCCCCCCEECCCEEEEEECCCC
GCKSGCSFCLTAKGGLKRNLSAGEIVGQILWIKKQNNIPYERRINIVYMGMGEPLDNLKN
CCCCCCCEEEECCCCCCCCCCHHHHHHHEEEEEECCCCCHHHEEEEEEEECCCCHHHHHH
VSKAVKILAQNEGLAISPRRQTISTSGLAKQIKELGQMNLGVLLAISLHAVNDELRTELM
HHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHCHHHHHHHH
PINKAYNIAAIMDAVREFPIDQRKRVMFEYLLIDGINDKLEHAKELVKLLNGIKAKVNLI
CCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEE
LFNPHEGGLYKRPSLENAIKFQDLLSNKGVTCTIRESKGLDISAACGQLKERAKEQ
EECCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKELVNILDFLPEELGEKIKPMFRVKQIYQWIYQKYANNFSDMSSLPKDLRLELAQNFHF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHCCCC
SPVKCVKNEQSKDGSIKYLFELVDGLRIESVLLPMKEEKIDAEGKRISHARYTICVSSQV
CCHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHCCCHHHHCCCCCCEECCCEEEEEECCCC
GCKSGCSFCLTAKGGLKRNLSAGEIVGQILWIKKQNNIPYERRINIVYMGMGEPLDNLKN
CCCCCCCEEEECCCCCCCCCCHHHHHHHEEEEEECCCCCHHHEEEEEEEECCCCHHHHHH
VSKAVKILAQNEGLAISPRRQTISTSGLAKQIKELGQMNLGVLLAISLHAVNDELRTELM
HHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHCHHHHHHHH
PINKAYNIAAIMDAVREFPIDQRKRVMFEYLLIDGINDKLEHAKELVKLLNGIKAKVNLI
CCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEE
LFNPHEGGLYKRPSLENAIKFQDLLSNKGVTCTIRESKGLDISAACGQLKERAKEQ
EECCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA