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The map label for this gene is yagA [C]

Identifier: 57234972

GI number: 57234972

Start: 160181

End: 161005

Strand: Direct

Name: yagA [C]

Synonym: DET0166

Alternate gene names: 57234972

Gene position: 160181-161005 (Clockwise)

Preceding gene: 57234973

Following gene: 57235084

Centisome position: 10.9

GC content: 52.61

Gene sequence:

>825_bases
GTGGAGTTTGCCAGGGGAAATCTTGGGCTCAGTGAAAGAAGAGCTTGTCTGCTTGTCGGTATTTCGCCATCCGCCTACCG
CTACAAGCCGAAACTAGACGATGATCTGGCCTTGCGCCAGCGGTTACGGGATCTGGCCGGGGAGCGGAAGCGCTTTGGCA
GCCCGCGGCTCCACATTATGCTTAAACGGGAAGGACTGGTGATCAATCACAAGCGGACCGAGCGAATCTACAAAGAAGAG
GGATTGGCGTTAAGAAGAAAACGCAAACGCAAAGGAACTGCCGTTAATCGAATCATATTGCCGGTGCCCGATAGGCCTAA
CCAAAGATGGAGCATGGACTTCGTTTCTGATTCCACCGTCACCGGGAGACGCTTCAGGACACTGACCATCGTCGATGACT
TCACCCGGGAATGCCCAGCGATCGAAGTGGATACATCTCTCGGTGGCGCCAGGGTGGTGAGTGTCCTGGAAAGGTTGGCT
GAGACTCGAGGCTTGCCCGAAGTCATCACTATCGACAATGGACCTGAGTTCGCCGGCCGGGCGCTTGATGAATGGGCTTA
TCGGCGGGGTGTAAAACTCAATTTCATACGGCCAGGCAAACCTATAGAAAATGCCTTCGCCGAGAGCTTTAACGGTAGAT
TCAGGGACGAATGCCTGAATGAAAACTGGTTCATGAGCTTGAAACAAGCCCGGGAAATCATCGAGGATTGGAGGATCGAC
TATAACGAAGTCAGGCCACACACTTCATTGAAAGGTCAAACACCTCAGGAGTATGCTGAGGCAAATTCTGGACTCTACTC
CGGAATGCTACTAAAGGTGGGGTAA

Upstream 100 bases:

>100_bases
GCCGTCTGAAGCAGATTGTGGCCGACCAGGCGCTTGATAATTGGGCGCTTAAAGAGCTTCTCGCAAAAAACTTCTAGAGC
CCAAGGTCAGGCGGGAGGCG

Downstream 100 bases:

>100_bases
GGTCAGCTTGACTAACGTTTAGTGGAAAACGCTCACTTCCATACGGTGCAACTCCAAGAATTCTTTTATTCAGCGATTGA
GCAAAATTGATTTCAAATTG

Product: ISDet2, transposase orfB

Products: NA

Alternate protein names: ORF1 [H]

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MEFARGNLGLSERRACLLVGISPSAYRYKPKLDDDLALRQRLRDLAGERKRFGSPRLHIMLKREGLVINHKRTERIYKEE
GLALRRKRKRKGTAVNRIILPVPDRPNQRWSMDFVSDSTVTGRRFRTLTIVDDFTRECPAIEVDTSLGGARVVSVLERLA
ETRGLPEVITIDNGPEFAGRALDEWAYRRGVKLNFIRPGKPIENAFAESFNGRFRDECLNENWFMSLKQAREIIEDWRID
YNEVRPHTSLKGQTPQEYAEANSGLYSGMLLKVG

Sequences:

>Translated_274_residues
MEFARGNLGLSERRACLLVGISPSAYRYKPKLDDDLALRQRLRDLAGERKRFGSPRLHIMLKREGLVINHKRTERIYKEE
GLALRRKRKRKGTAVNRIILPVPDRPNQRWSMDFVSDSTVTGRRFRTLTIVDDFTRECPAIEVDTSLGGARVVSVLERLA
ETRGLPEVITIDNGPEFAGRALDEWAYRRGVKLNFIRPGKPIENAFAESFNGRFRDECLNENWFMSLKQAREIIEDWRID
YNEVRPHTSLKGQTPQEYAEANSGLYSGMLLKVG
>Mature_274_residues
MEFARGNLGLSERRACLLVGISPSAYRYKPKLDDDLALRQRLRDLAGERKRFGSPRLHIMLKREGLVINHKRTERIYKEE
GLALRRKRKRKGTAVNRIILPVPDRPNQRWSMDFVSDSTVTGRRFRTLTIVDDFTRECPAIEVDTSLGGARVVSVLERLA
ETRGLPEVITIDNGPEFAGRALDEWAYRRGVKLNFIRPGKPIENAFAESFNGRFRDECLNENWFMSLKQAREIIEDWRID
YNEVRPHTSLKGQTPQEYAEANSGLYSGMLLKVG

Specific function: Unknown

COG id: COG2801

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Escherichia coli, GI1786462, Length=174, Percent_Identity=31.6091954022989, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1789981, Length=244, Percent_Identity=27.0491803278689, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 31550; Mature: 31550

Theoretical pI: Translated: 10.32; Mature: 10.32

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFARGNLGLSERRACLLVGISPSAYRYKPKLDDDLALRQRLRDLAGERKRFGSPRLHIM
CCCCCCCCCCCCCCEEEEEECCCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEE
LKREGLVINHKRTERIYKEEGLALRRKRKRKGTAVNRIILPVPDRPNQRWSMDFVSDSTV
EECCCEEEECHHHHHHHHHHCHHHHHHHHHCCCCCCEEEEECCCCCCCCEEEEECCCCCC
TGRRFRTLTIVDDFTRECPAIEVDTSLGGARVVSVLERLAETRGLPEVITIDNGPEFAGR
CCCEEEEEEEEEHHHHHCCCEEEECCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHH
ALDEWAYRRGVKLNFIRPGKPIENAFAESFNGRFRDECLNENWFMSLKQAREIIEDWRID
HHHHHHHHCCCEEEEECCCCCHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHCCC
YNEVRPHTSLKGQTPQEYAEANSGLYSGMLLKVG
HHHCCCCCCCCCCCHHHHHHHCCCHHCCEEEEEC
>Mature Secondary Structure
MEFARGNLGLSERRACLLVGISPSAYRYKPKLDDDLALRQRLRDLAGERKRFGSPRLHIM
CCCCCCCCCCCCCCEEEEEECCCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEE
LKREGLVINHKRTERIYKEEGLALRRKRKRKGTAVNRIILPVPDRPNQRWSMDFVSDSTV
EECCCEEEECHHHHHHHHHHCHHHHHHHHHCCCCCCEEEEECCCCCCCCEEEEECCCCCC
TGRRFRTLTIVDDFTRECPAIEVDTSLGGARVVSVLERLAETRGLPEVITIDNGPEFAGR
CCCEEEEEEEEEHHHHHCCCEEEECCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHH
ALDEWAYRRGVKLNFIRPGKPIENAFAESFNGRFRDECLNENWFMSLKQAREIIEDWRID
HHHHHHHHCCCEEEEECCCCCHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHCCC
YNEVRPHTSLKGQTPQEYAEANSGLYSGMLLKVG
HHHCCCCCCCCCCCHHHHHHHCCCHHCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1718819 [H]