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The map label for this gene is iscS [H]

Identifier: 57234971

GI number: 57234971

Start: 237790

End: 238941

Strand: Direct

Name: iscS [H]

Synonym: DET0248

Alternate gene names: 57234971

Gene position: 237790-238941 (Clockwise)

Preceding gene: 57234942

Following gene: 57234970

Centisome position: 16.18

GC content: 56.68

Gene sequence:

>1152_bases
ATGGATGAGCTGATTTACCTGGATAATGCCGCCAGTACCCCGGTTGACCCGAGGGTAGTTACGGCTATGCTGCCTTACTT
TACCTGGCACTACGGAAACCCCTCGGCTATTCACCCCATGGGTCGCAGGGCGGACGAGGCTCTGGAAACCGCCCGTGAGC
AGGTGGCCGCCCTGATTAACTGCCAGCCGGAAGAGGTTATCTTTACCAGCGGCGGCACCGAATCAGACAATCTGGCTATC
AGCGGTGCGGCGATGGCTGGCCGGGGCAGACACATTATTACCTCGGAGATTGAGCACCATGCCGTGCTCTTAGCCTGTCA
TGCCCTGGAAAAGAAGGGCTTTGAGGTTACCTATCTGCCGGTGGACTCCTTCGGGCAGGTTTCTCCTAAGGACGTGGAGG
CGGCCATTACTCCCCAAACGGCTTTGGTAAGCATTATGGCGGCCAATAATGTAATCGGCACCCTTCAGCCTGTAGCTGAA
ATAGGGGCTGTCTGCCGCCGTCAGGGAGTTATTTTCCATACCGATGCCGTCCAGATGGCCGGTCACCTGCCGCTGGATGT
AAAGCGGGATAACATAGACCTTTTATCCCTGTCTGCCCACAAGTTTAACGGCCCCAAAGGGGTGGGAATACTCTATGCCA
AGGGCGATGTGCCTTTAGCCCCGCTTATTCTGGGCGGTGGGCAGGAAAGCGGCCTGCGTTCGGGTACGGAAAATGTGCCC
GGTATTGCGGGACTGGGGCTGGCAGCCGGGATAGCCGCACTGGAAATGCAAGAAGAGGCGGGCAGGGTTTCCTCCCTTCG
TAAAAAACTGGCCGAAGCCATACTGGGGTCTATTCCCGGCAGCCGCCTTAACGGCCACCCCCAAAAGCGTCTGCCCGGTA
ATCTGAATATCAGTTTTGAAGGAGTGGAGGGAGAATACCTGAGCAAGGAACTAGCCAAGCAGGGTATCTGCGTATCTACC
GGTTCGGCCTGCAGTTCGGCAAGCCACGAAGCCCCTTATGTGCTTCTGGCGACCGGCCTTGAGCGTGATCTGGCTAACTG
CTCTATCCGCCTTTCTCTGGGTAAAATGACCACCCCGGAGCATATTCAAAAAACCGCAGATACTTTGAGTGCGGTAGTCA
GCCAAATCCGCCGGCAAACCCCGGTCTGGTAG

Upstream 100 bases:

>100_bases
ATAAGCTGGGGGCGGAACTGGATGCCTCTATTGTGGCTTACTCCCGGGGTAAAACCGCCCGCCCTGCCGGGCAGTCCTGC
AGGATATAGGGGGAAATACT

Downstream 100 bases:

>100_bases
GAGGAAAAAGCTTATGCAACTGAAAAATACAGGGTATTCACCGGTAACTTTGGAAAGGGAGGTCTGTTCTTTGCCGGGTG
TAAAAGCCGAAGTGTTTTCC

Product: cysteine desulfurase

Products: NA

Alternate protein names: NifS protein homolog [H]

Number of amino acids: Translated: 383; Mature: 383

Protein sequence:

>383_residues
MDELIYLDNAASTPVDPRVVTAMLPYFTWHYGNPSAIHPMGRRADEALETAREQVAALINCQPEEVIFTSGGTESDNLAI
SGAAMAGRGRHIITSEIEHHAVLLACHALEKKGFEVTYLPVDSFGQVSPKDVEAAITPQTALVSIMAANNVIGTLQPVAE
IGAVCRRQGVIFHTDAVQMAGHLPLDVKRDNIDLLSLSAHKFNGPKGVGILYAKGDVPLAPLILGGGQESGLRSGTENVP
GIAGLGLAAGIAALEMQEEAGRVSSLRKKLAEAILGSIPGSRLNGHPQKRLPGNLNISFEGVEGEYLSKELAKQGICVST
GSACSSASHEAPYVLLATGLERDLANCSIRLSLGKMTTPEHIQKTADTLSAVVSQIRRQTPVW

Sequences:

>Translated_383_residues
MDELIYLDNAASTPVDPRVVTAMLPYFTWHYGNPSAIHPMGRRADEALETAREQVAALINCQPEEVIFTSGGTESDNLAI
SGAAMAGRGRHIITSEIEHHAVLLACHALEKKGFEVTYLPVDSFGQVSPKDVEAAITPQTALVSIMAANNVIGTLQPVAE
IGAVCRRQGVIFHTDAVQMAGHLPLDVKRDNIDLLSLSAHKFNGPKGVGILYAKGDVPLAPLILGGGQESGLRSGTENVP
GIAGLGLAAGIAALEMQEEAGRVSSLRKKLAEAILGSIPGSRLNGHPQKRLPGNLNISFEGVEGEYLSKELAKQGICVST
GSACSSASHEAPYVLLATGLERDLANCSIRLSLGKMTTPEHIQKTADTLSAVVSQIRRQTPVW
>Mature_383_residues
MDELIYLDNAASTPVDPRVVTAMLPYFTWHYGNPSAIHPMGRRADEALETAREQVAALINCQPEEVIFTSGGTESDNLAI
SGAAMAGRGRHIITSEIEHHAVLLACHALEKKGFEVTYLPVDSFGQVSPKDVEAAITPQTALVSIMAANNVIGTLQPVAE
IGAVCRRQGVIFHTDAVQMAGHLPLDVKRDNIDLLSLSAHKFNGPKGVGILYAKGDVPLAPLILGGGQESGLRSGTENVP
GIAGLGLAAGIAALEMQEEAGRVSSLRKKLAEAILGSIPGSRLNGHPQKRLPGNLNISFEGVEGEYLSKELAKQGICVST
GSACSSASHEAPYVLLATGLERDLANCSIRLSLGKMTTPEHIQKTADTLSAVVSQIRRQTPVW

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=384, Percent_Identity=47.9166666666667, Blast_Score=356, Evalue=2e-98,
Organism=Homo sapiens, GI156713448, Length=416, Percent_Identity=33.8942307692308, Blast_Score=214, Evalue=8e-56,
Organism=Escherichia coli, GI48994898, Length=386, Percent_Identity=47.4093264248705, Blast_Score=326, Evalue=1e-90,
Organism=Escherichia coli, GI1787970, Length=345, Percent_Identity=30.1449275362319, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1789175, Length=352, Percent_Identity=30.3977272727273, Blast_Score=109, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI25143064, Length=385, Percent_Identity=48.3116883116883, Blast_Score=334, Evalue=4e-92,
Organism=Caenorhabditis elegans, GI17533177, Length=328, Percent_Identity=33.8414634146341, Blast_Score=157, Evalue=9e-39,
Organism=Saccharomyces cerevisiae, GI6319831, Length=384, Percent_Identity=46.6145833333333, Blast_Score=332, Evalue=7e-92,
Organism=Drosophila melanogaster, GI20129463, Length=384, Percent_Identity=48.9583333333333, Blast_Score=347, Evalue=7e-96,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR010240
- InterPro:   IPR017772
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 40499; Mature: 40499

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDELIYLDNAASTPVDPRVVTAMLPYFTWHYGNPSAIHPMGRRADEALETAREQVAALIN
CCCEEEECCCCCCCCCHHHHHHHHHHHEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHC
CQPEEVIFTSGGTESDNLAISGAAMAGRGRHIITSEIEHHAVLLACHALEKKGFEVTYLP
CCCCEEEEECCCCCCCCEEEEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHCCCEEEEEE
VDSFGQVSPKDVEAAITPQTALVSIMAANNVIGTLQPVAEIGAVCRRQGVIFHTDAVQMA
CCCCCCCCCCCCEEEECCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCEEEEEHHHHHC
GHLPLDVKRDNIDLLSLSAHKFNGPKGVGILYAKGDVPLAPLILGGGQESGLRSGTENVP
CCCCCEEECCCCEEEEEECCCCCCCCCEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCC
GIAGLGLAAGIAALEMQEEAGRVSSLRKKLAEAILGSIPGSRLNGHPQKRLPGNLNISFE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCCCCEEEEE
GVEGEYLSKELAKQGICVSTGSACSSASHEAPYVLLATGLERDLANCSIRLSLGKMTTPE
CCCCHHHHHHHHHCCCEEECCCCCCCCCCCCCEEEEECCCHHHHHCCEEEEEECCCCCHH
HIQKTADTLSAVVSQIRRQTPVW
HHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDELIYLDNAASTPVDPRVVTAMLPYFTWHYGNPSAIHPMGRRADEALETAREQVAALIN
CCCEEEECCCCCCCCCHHHHHHHHHHHEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHC
CQPEEVIFTSGGTESDNLAISGAAMAGRGRHIITSEIEHHAVLLACHALEKKGFEVTYLP
CCCCEEEEECCCCCCCCEEEEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHCCCEEEEEE
VDSFGQVSPKDVEAAITPQTALVSIMAANNVIGTLQPVAEIGAVCRRQGVIFHTDAVQMA
CCCCCCCCCCCCEEEECCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCEEEEEHHHHHC
GHLPLDVKRDNIDLLSLSAHKFNGPKGVGILYAKGDVPLAPLILGGGQESGLRSGTENVP
CCCCCEEECCCCEEEEEECCCCCCCCCEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCC
GIAGLGLAAGIAALEMQEEAGRVSSLRKKLAEAILGSIPGSRLNGHPQKRLPGNLNISFE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCCCCEEEEE
GVEGEYLSKELAKQGICVSTGSACSSASHEAPYVLLATGLERDLANCSIRLSLGKMTTPE
CCCCHHHHHHHHHCCCEEECCCCCCCCCCCCCEEEEECCCHHHHHCCEEEEEECCCCCHH
HIQKTADTLSAVVSQIRRQTPVW
HHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10930739 [H]