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The map label for this gene is mraW

Identifier: 57234909

GI number: 57234909

Start: 325087

End: 326136

Strand: Direct

Name: mraW

Synonym: DET0341

Alternate gene names: 57234909

Gene position: 325087-326136 (Clockwise)

Preceding gene: 57234910

Following gene: 57234908

Centisome position: 22.12

GC content: 51.81

Gene sequence:

>1050_bases
GTGACTGAGTATCCTCACATACCGGTTATGCTGGAGGAGTCCATACAGGGGCTGGGGGTTATACCCGGCGGCCGTTATGT
GGATTGCACTTTGGGGGCTGGCGGTCACTCAGAAGCTATTTTGGAGCACAGTTACCCCGGCGGACAGCTTTTATCTATAG
ATGCAGACCCCAAGGCTATTGCTTTGGCTGCCGAACGGCTTAAGTGTTTCGGCAGTTCGGTTTTACTGGTAAATGACAAC
TTTGCCAATCTGAAAGATATATGCCAGCGGTATGAATATATGCCGGTGCATGGCATTTTATTTGATTTGGGGCTTTCTTC
CATGCAGCTTGACCGGGAGGAATCCGGGTTCAGTTTTCAGACCGAAGCCCCGCTGGATATGCGTTTTTCACCCGGCCAGG
AGCTATCGGCGGCGGATATCGTTAATACTTATGACCTTGCCGGGCTGTCAGATTTAATCTGGAAATACGGTGAAGAGCCT
TTCAGCCGGCGGATTGCCAGGGCTATTTTAGAGAAAAGACCGTTTAAAACAACTACCGAATTGGCGTCTGTCATAGAAAA
AGCGGTTGGGGGGCGTCACGGGCGGATTCATCCTGCTACCCGGACTTTCCAGGCTTTGCGGATAGCGGTAAACGAAGAGC
TTTCGCACCTTGAGAGTGCCCTTGCACAGGCTCAGAGCCTTTTGGGACATGGCGGGCGGCTGGTAGTGATAAGCTACCAT
TCGCTTGAAGACCGTATTGTAAAGCAGTATTTCCAGAAAGAAGCCAAGGGCTGTATCTGCCCTGATGATATACCCCAGTG
TGTTTGCAACCACAAGCCTTCACTTAGGCTGATAAACCGCAGGGTAATTACCCCTTCGGATGAGGAAATATCCCGAAACC
CGCGTAGCCGGAGTGCCAAGATGCGGGTGGCGGAACGGATTATACAACCCGGCGAGGGGCGTTTTTTTCACAGCCGGGCT
AAGGGATTAGTTAATCATGTCAGCGAAACTGGCTCTGTTCGGTACGGACAGGCTAAACATAAGGGGGTGGTCCAGAGGGG
AGGCAGCTGA

Upstream 100 bases:

>100_bases
TATAGAGATATGGGATAAAGAGACCTGGGAGTCTGAGAAGAAGGCCAGCCAGGAGCAGGCCTGGCAGATAATAGAAACGC
TTGAGGGGAACTAGGCGGAA

Downstream 100 bases:

>100_bases
TGTGCGCAAAGGAATAACGTTCCCGTAAGGGATTTTTAGATACACGGTTTTGCAAATGGAGGGAGATATATGAAAAACAG
AATCGTTACAGCGATTGATG

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 349; Mature: 348

Protein sequence:

>349_residues
MTEYPHIPVMLEESIQGLGVIPGGRYVDCTLGAGGHSEAILEHSYPGGQLLSIDADPKAIALAAERLKCFGSSVLLVNDN
FANLKDICQRYEYMPVHGILFDLGLSSMQLDREESGFSFQTEAPLDMRFSPGQELSAADIVNTYDLAGLSDLIWKYGEEP
FSRRIARAILEKRPFKTTTELASVIEKAVGGRHGRIHPATRTFQALRIAVNEELSHLESALAQAQSLLGHGGRLVVISYH
SLEDRIVKQYFQKEAKGCICPDDIPQCVCNHKPSLRLINRRVITPSDEEISRNPRSRSAKMRVAERIIQPGEGRFFHSRA
KGLVNHVSETGSVRYGQAKHKGVVQRGGS

Sequences:

>Translated_349_residues
MTEYPHIPVMLEESIQGLGVIPGGRYVDCTLGAGGHSEAILEHSYPGGQLLSIDADPKAIALAAERLKCFGSSVLLVNDN
FANLKDICQRYEYMPVHGILFDLGLSSMQLDREESGFSFQTEAPLDMRFSPGQELSAADIVNTYDLAGLSDLIWKYGEEP
FSRRIARAILEKRPFKTTTELASVIEKAVGGRHGRIHPATRTFQALRIAVNEELSHLESALAQAQSLLGHGGRLVVISYH
SLEDRIVKQYFQKEAKGCICPDDIPQCVCNHKPSLRLINRRVITPSDEEISRNPRSRSAKMRVAERIIQPGEGRFFHSRA
KGLVNHVSETGSVRYGQAKHKGVVQRGGS
>Mature_348_residues
TEYPHIPVMLEESIQGLGVIPGGRYVDCTLGAGGHSEAILEHSYPGGQLLSIDADPKAIALAAERLKCFGSSVLLVNDNF
ANLKDICQRYEYMPVHGILFDLGLSSMQLDREESGFSFQTEAPLDMRFSPGQELSAADIVNTYDLAGLSDLIWKYGEEPF
SRRIARAILEKRPFKTTTELASVIEKAVGGRHGRIHPATRTFQALRIAVNEELSHLESALAQAQSLLGHGGRLVVISYHS
LEDRIVKQYFQKEAKGCICPDDIPQCVCNHKPSLRLINRRVITPSDEEISRNPRSRSAKMRVAERIIQPGEGRFFHSRAK
GLVNHVSETGSVRYGQAKHKGVVQRGGS

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=337, Percent_Identity=36.4985163204748, Blast_Score=188, Evalue=6e-48,
Organism=Homo sapiens, GI165377202, Length=188, Percent_Identity=36.7021276595745, Blast_Score=114, Evalue=1e-25,
Organism=Escherichia coli, GI1786270, Length=314, Percent_Identity=42.0382165605096, Blast_Score=232, Evalue=2e-62,
Organism=Drosophila melanogaster, GI62472493, Length=333, Percent_Identity=35.1351351351351, Blast_Score=186, Evalue=3e-47,
Organism=Drosophila melanogaster, GI28571637, Length=333, Percent_Identity=35.1351351351351, Blast_Score=186, Evalue=3e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_DEHE1 (Q3Z9L1)

Other databases:

- EMBL:   CP000027
- RefSeq:   YP_181088.1
- ProteinModelPortal:   Q3Z9L1
- SMR:   Q3Z9L1
- STRING:   Q3Z9L1
- GeneID:   3230378
- GenomeReviews:   CP000027_GR
- KEGG:   det:DET0341
- NMPDR:   fig|243164.3.peg.352
- TIGR:   DET0341
- eggNOG:   COG0275
- HOGENOM:   HBG302779
- OMA:   PQLDDPE
- PhylomeDB:   Q3Z9L1
- ProtClustDB:   PRK00050
- BioCyc:   DETH243164:DET_0341-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 38575; Mature: 38443

Theoretical pI: Translated: 7.86; Mature: 7.86

Prosite motif: NA

Important sites: BINDING 54-54 BINDING 81-81 BINDING 102-102 BINDING 109-109

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEYPHIPVMLEESIQGLGVIPGGRYVDCTLGAGGHSEAILEHSYPGGQLLSIDADPKAI
CCCCCCCCEEHHCCCCCCEECCCCCEEEEEECCCCCCCHHHCCCCCCCEEEEECCCCHHH
ALAAERLKCFGSSVLLVNDNFANLKDICQRYEYMPVHGILFDLGLSSMQLDREESGFSFQ
HHHHHHHHHCCCEEEEEECCCCCHHHHHHHHCCCCHHHHHHHHCCCHHHCCCCCCCCEEE
TEAPLDMRFSPGQELSAADIVNTYDLAGLSDLIWKYGEEPFSRRIARAILEKRPFKTTTE
CCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHH
LASVIEKAVGGRHGRIHPATRTFQALRIAVNEELSHLESALAQAQSLLGHGGRLVVISYH
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEC
SLEDRIVKQYFQKEAKGCICPDDIPQCVCNHKPSLRLINRRVITPSDEEISRNPRSRSAK
CHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCEEECCCEECCCCHHHHHCCCCHHHHH
MRVAERIIQPGEGRFFHSRAKGLVNHVSETGSVRYGQAKHKGVVQRGGS
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCEEECCHHHHHHHHCCCC
>Mature Secondary Structure 
TEYPHIPVMLEESIQGLGVIPGGRYVDCTLGAGGHSEAILEHSYPGGQLLSIDADPKAI
CCCCCCCEEHHCCCCCCEECCCCCEEEEEECCCCCCCHHHCCCCCCCEEEEECCCCHHH
ALAAERLKCFGSSVLLVNDNFANLKDICQRYEYMPVHGILFDLGLSSMQLDREESGFSFQ
HHHHHHHHHCCCEEEEEECCCCCHHHHHHHHCCCCHHHHHHHHCCCHHHCCCCCCCCEEE
TEAPLDMRFSPGQELSAADIVNTYDLAGLSDLIWKYGEEPFSRRIARAILEKRPFKTTTE
CCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHH
LASVIEKAVGGRHGRIHPATRTFQALRIAVNEELSHLESALAQAQSLLGHGGRLVVISYH
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEC
SLEDRIVKQYFQKEAKGCICPDDIPQCVCNHKPSLRLINRRVITPSDEEISRNPRSRSAK
CHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCEEECCCEECCCCHHHHHCCCCHHHHH
MRVAERIIQPGEGRFFHSRAKGLVNHVSETGSVRYGQAKHKGVVQRGGS
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCEEECCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA