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The map label for this gene is pheS

Identifier: 57234860

GI number: 57234860

Start: 345870

End: 346910

Strand: Direct

Name: pheS

Synonym: DET0365

Alternate gene names: 57234860

Gene position: 345870-346910 (Clockwise)

Preceding gene: 57234861

Following gene: 57234859

Centisome position: 23.53

GC content: 51.39

Gene sequence:

>1041_bases
ATGGAAGAAATTAAGAATATCACCGAAATTACCCAAAGTGCCCTTGCCGAACTGGAAGCCATAACGGAACTTAAAGACCT
TGAAGCGTGGCGTGTCCGGTATCTGGGCAAGAAAAGCCTGCTGACCGGGGCACTCCGGAATCTGGCCAGTTTGCCTATAG
AAGAACGCAAAGCGGCCGGAGCGGCAGCCAACGAGGCCAAAGCCGCACTTGAAGCTGCTTTTTTAGAGAAAGAGCAGCTA
TCTAAAGAAAAACAGTTTGCCAGCCGCAGTGAGGGGCTGGATATTACTTTGCCCGGCAGACCCTGGCCTATCGGCCGGAT
ACACCCCTTGACCCAGGTTACCAATGAAGTGACTGCTATCTTCTCCACTCTGGGTTTTTCCGTACTGGAAGGGCCGGAAA
TAGAAGATGACTATCATAATTTTGAGGCTCTTAATATTCCGGAAGACCACCCTGCCAGAGAAAATATGCAGACCTTCTGG
ATAGACCGGCCTAATGAAGACGGCAGGCTGGATACTTTGCTTCGCACCCATACCTCTCCCATGCAGGTGCGCTATATGGA
GAAAAACAAACCCCCCATACGGATAGTTGTTCCGGGCAAGGTTTACAGGTATGAAGCTACCGATGCCACCCATATTCCCA
TGTTTACCCAGGTGGAAGGGTTGGTGGTGGACGAAGGCATTTCCATGGCTCACCTGAAAGGCACTTTGATGGAGTTTTGC
CGCCGCTTTTTCGGGGCTAACCGCAAGGTGCGTTTCCGCTGTGACTACTTCCCCTTTGTAGAGCCGGGTGTGGAGGTAGC
GGTAAGCTGTACTTGCGGGGGCAAAAAAGAATGCAGCGTCTGCCATGGTTCCGGCTGGCTGGAAATACTGGGGGCGGGCA
TGGTACACCCCAGGGTGCTGGAGAGGGTAGGTATAGATTCGGAAAAATATACCGGCTTTGCCTTTGGCATGGGGCTGGAA
AGATTGCCCATGCTGAGATACGGGATTGATGATATCCGTCTGTTCTACTCTAACGATACTAGATTCTTGAGGCAGTTTTA
A

Upstream 100 bases:

>100_bases
CTGATTAAGGCGGATATACCTGACATAATGTTAGTTTGTGTTGACCATTCAAAGCTAATAGCATATAATTTTTATAAAAA
TTTTGATGTGAAGCTGGGCT

Downstream 100 bases:

>100_bases
TATGAAAATACCTTTGAAGTGGCTTAAAGAATATCTTCCCACAGATATGTCGCCGGCCGAACTGGCCGAACGGATGACTA
TGGCCGGTACTGAGGTGACA

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MEEIKNITEITQSALAELEAITELKDLEAWRVRYLGKKSLLTGALRNLASLPIEERKAAGAAANEAKAALEAAFLEKEQL
SKEKQFASRSEGLDITLPGRPWPIGRIHPLTQVTNEVTAIFSTLGFSVLEGPEIEDDYHNFEALNIPEDHPARENMQTFW
IDRPNEDGRLDTLLRTHTSPMQVRYMEKNKPPIRIVVPGKVYRYEATDATHIPMFTQVEGLVVDEGISMAHLKGTLMEFC
RRFFGANRKVRFRCDYFPFVEPGVEVAVSCTCGGKKECSVCHGSGWLEILGAGMVHPRVLERVGIDSEKYTGFAFGMGLE
RLPMLRYGIDDIRLFYSNDTRFLRQF

Sequences:

>Translated_346_residues
MEEIKNITEITQSALAELEAITELKDLEAWRVRYLGKKSLLTGALRNLASLPIEERKAAGAAANEAKAALEAAFLEKEQL
SKEKQFASRSEGLDITLPGRPWPIGRIHPLTQVTNEVTAIFSTLGFSVLEGPEIEDDYHNFEALNIPEDHPARENMQTFW
IDRPNEDGRLDTLLRTHTSPMQVRYMEKNKPPIRIVVPGKVYRYEATDATHIPMFTQVEGLVVDEGISMAHLKGTLMEFC
RRFFGANRKVRFRCDYFPFVEPGVEVAVSCTCGGKKECSVCHGSGWLEILGAGMVHPRVLERVGIDSEKYTGFAFGMGLE
RLPMLRYGIDDIRLFYSNDTRFLRQF
>Mature_346_residues
MEEIKNITEITQSALAELEAITELKDLEAWRVRYLGKKSLLTGALRNLASLPIEERKAAGAAANEAKAALEAAFLEKEQL
SKEKQFASRSEGLDITLPGRPWPIGRIHPLTQVTNEVTAIFSTLGFSVLEGPEIEDDYHNFEALNIPEDHPARENMQTFW
IDRPNEDGRLDTLLRTHTSPMQVRYMEKNKPPIRIVVPGKVYRYEATDATHIPMFTQVEGLVVDEGISMAHLKGTLMEFC
RRFFGANRKVRFRCDYFPFVEPGVEVAVSCTCGGKKECSVCHGSGWLEILGAGMVHPRVLERVGIDSEKYTGFAFGMGLE
RLPMLRYGIDDIRLFYSNDTRFLRQF

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4758340, Length=267, Percent_Identity=32.2097378277154, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI5729820, Length=296, Percent_Identity=29.7297297297297, Blast_Score=114, Evalue=1e-25,
Organism=Escherichia coli, GI1788007, Length=345, Percent_Identity=48.4057971014493, Blast_Score=325, Evalue=2e-90,
Organism=Caenorhabditis elegans, GI17508957, Length=266, Percent_Identity=29.6992481203008, Blast_Score=124, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI32563657, Length=266, Percent_Identity=29.6992481203008, Blast_Score=124, Evalue=8e-29,
Organism=Caenorhabditis elegans, GI32566635, Length=263, Percent_Identity=31.5589353612167, Blast_Score=120, Evalue=8e-28,
Organism=Saccharomyces cerevisiae, GI6321087, Length=372, Percent_Identity=26.8817204301075, Blast_Score=127, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6325304, Length=286, Percent_Identity=26.5734265734266, Blast_Score=90, Evalue=6e-19,
Organism=Drosophila melanogaster, GI18858079, Length=277, Percent_Identity=30.6859205776173, Blast_Score=121, Evalue=6e-28,
Organism=Drosophila melanogaster, GI17137424, Length=255, Percent_Identity=28.6274509803922, Blast_Score=105, Evalue=3e-23,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFA_DEHE1 (Q3Z9I8)

Other databases:

- EMBL:   CP000027
- RefSeq:   YP_181111.1
- ProteinModelPortal:   Q3Z9I8
- SMR:   Q3Z9I8
- STRING:   Q3Z9I8
- GeneID:   3230329
- GenomeReviews:   CP000027_GR
- KEGG:   det:DET0365
- NMPDR:   fig|243164.3.peg.503
- TIGR:   DET0365
- eggNOG:   COG0016
- HOGENOM:   HBG284353
- OMA:   FRASYFP
- PhylomeDB:   Q3Z9I8
- ProtClustDB:   PRK00488
- BioCyc:   DETH243164:DET_0365-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00281
- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978
- PANTHER:   PTHR11538
- TIGRFAMs:   TIGR00468

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d; SSF46589 tRNA_binding_arm

EC number: =6.1.1.20

Molecular weight: Translated: 39133; Mature: 39133

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEIKNITEITQSALAELEAITELKDLEAWRVRYLGKKSLLTGALRNLASLPIEERKAAG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCHHHHHHCC
AAANEAKAALEAAFLEKEQLSKEKQFASRSEGLDITLPGRPWPIGRIHPLTQVTNEVTAI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHHH
FSTLGFSVLEGPEIEDDYHNFEALNIPEDHPARENMQTFWIDRPNEDGRLDTLLRTHTSP
HHHHCHHHHCCCCCCCCCCCCEEECCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHCCCC
MQVRYMEKNKPPIRIVVPGKVYRYEATDATHIPMFTQVEGLVVDEGISMAHLKGTLMEFC
EEEEEEECCCCCEEEEECCEEEEEECCCCCCCCCHHCCCCEEEECCCCHHHHHHHHHHHH
RRFFGANRKVRFRCDYFPFVEPGVEVAVSCTCGGKKECSVCHGSGWLEILGAGMVHPRVL
HHHHCCCCEEEEEECCCCCCCCCCEEEEEEECCCCCCCCEECCCCHHHHHCCCCCCHHHH
ERVGIDSEKYTGFAFGMGLERLPMLRYGIDDIRLFYSNDTRFLRQF
HHHCCCCCCCCEEEECCCHHHHHHHHHCCCEEEEEECCCHHHHHCC
>Mature Secondary Structure
MEEIKNITEITQSALAELEAITELKDLEAWRVRYLGKKSLLTGALRNLASLPIEERKAAG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCHHHHHHCC
AAANEAKAALEAAFLEKEQLSKEKQFASRSEGLDITLPGRPWPIGRIHPLTQVTNEVTAI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHHH
FSTLGFSVLEGPEIEDDYHNFEALNIPEDHPARENMQTFWIDRPNEDGRLDTLLRTHTSP
HHHHCHHHHCCCCCCCCCCCCEEECCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHCCCC
MQVRYMEKNKPPIRIVVPGKVYRYEATDATHIPMFTQVEGLVVDEGISMAHLKGTLMEFC
EEEEEEECCCCCEEEEECCEEEEEECCCCCCCCCHHCCCCEEEECCCCHHHHHHHHHHHH
RRFFGANRKVRFRCDYFPFVEPGVEVAVSCTCGGKKECSVCHGSGWLEILGAGMVHPRVL
HHHHCCCCEEEEEECCCCCCCCCCEEEEEEECCCCCCCCEECCCCHHHHHCCCCCCHHHH
ERVGIDSEKYTGFAFGMGLERLPMLRYGIDDIRLFYSNDTRFLRQF
HHHCCCCCCCCEEEECCCHHHHHHHHHCCCEEEEEECCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA