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The map label for this gene is truA [H]

Identifier: 57234742

GI number: 57234742

Start: 460538

End: 461284

Strand: Direct

Name: truA [H]

Synonym: DET0504

Alternate gene names: 57234742

Gene position: 460538-461284 (Clockwise)

Preceding gene: 57234743

Following gene: 57234741

Centisome position: 31.34

GC content: 49.93

Gene sequence:

>747_bases
GTGTTATTGGTAATTGAATACGAGGGTACCCGGTATTCAGGCTTTCAGGTTCAGGATAAAGGGGCCACTATACAGGGAGA
GCTGGAAACAGCTATCCGGAAATTAACAAGTGAACAGACCAGAGTGAGCGGTTCCGGAAGAACTGATGCCGGGGTACACG
CGGCTTACCAGGTAGTCAGCTACCTCTCGGAATCTTCACTGGGATTGGACAAGATAAAGGGCGGGTTAAATTTTTACCTG
CCGCCGGACATAGCCGTAAAAGAAGTCCACTTCGTAGCTGAAAGCTTTGATGTGAGACGCCGGGCTATCAGCCGGGAGTA
CTCTTACCAAATATTAAATAGTAAAACTCGCTCGCCGCTTGCCAGGCTTCAGGCTTGGCAGGTGCCTTACAAGCTAGATA
TAAAGCTAATGGAACAGGCTTTTAAGATGCTTGAAGGGGAGCATGACTTTATCGCTTTTGCCTCCAGTCTGGGGCCGGAG
ATAAAGAAAACTGTCCGCCGGGTTAACTGGACCCGGTTAGGTATTAGGGAGGATAGGCTGATATTCAATATAGAGGCCAA
TTCCTTTCTTACCCACCAGGTACGGAATACTATCGGGACACTGGTGGAGTTAGGACGCGGGACTATGAGCATGAGTGAGT
TTGAGGCATTATTTGAGGCTAAAACCCCCGGACTGGCGGGGCCGGCAGCGCCGGCTTGTGGACTGAGCCTCGTTCGGGTA
AACTATAGAGAACCTTTTGGGAGTTAA

Upstream 100 bases:

>100_bases
CCCGAATGACCAAGCTGGGTCCCCGTCAGGGTGACGGTGCGGAGATGGTTAAGCTGGAACTTGTCAAGTAACTTTTGGGA
GGGGTATTATCTCACCCAGA

Downstream 100 bases:

>100_bases
GATGAATACGTACACAGTTAAAGCTAGTGACATAAAACGGGATTGGCACGTGATAGACGCTTCCGGCCGTGTGCTGGGTG
AGGTGGCTGCCGAAGCCGCT

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MLLVIEYEGTRYSGFQVQDKGATIQGELETAIRKLTSEQTRVSGSGRTDAGVHAAYQVVSYLSESSLGLDKIKGGLNFYL
PPDIAVKEVHFVAESFDVRRRAISREYSYQILNSKTRSPLARLQAWQVPYKLDIKLMEQAFKMLEGEHDFIAFASSLGPE
IKKTVRRVNWTRLGIREDRLIFNIEANSFLTHQVRNTIGTLVELGRGTMSMSEFEALFEAKTPGLAGPAAPACGLSLVRV
NYREPFGS

Sequences:

>Translated_248_residues
MLLVIEYEGTRYSGFQVQDKGATIQGELETAIRKLTSEQTRVSGSGRTDAGVHAAYQVVSYLSESSLGLDKIKGGLNFYL
PPDIAVKEVHFVAESFDVRRRAISREYSYQILNSKTRSPLARLQAWQVPYKLDIKLMEQAFKMLEGEHDFIAFASSLGPE
IKKTVRRVNWTRLGIREDRLIFNIEANSFLTHQVRNTIGTLVELGRGTMSMSEFEALFEAKTPGLAGPAAPACGLSLVRV
NYREPFGS
>Mature_248_residues
MLLVIEYEGTRYSGFQVQDKGATIQGELETAIRKLTSEQTRVSGSGRTDAGVHAAYQVVSYLSESSLGLDKIKGGLNFYL
PPDIAVKEVHFVAESFDVRRRAISREYSYQILNSKTRSPLARLQAWQVPYKLDIKLMEQAFKMLEGEHDFIAFASSLGPE
IKKTVRRVNWTRLGIREDRLIFNIEANSFLTHQVRNTIGTLVELGRGTMSMSEFEALFEAKTPGLAGPAAPACGLSLVRV
NYREPFGS

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=270, Percent_Identity=30.3703703703704, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI70166634, Length=278, Percent_Identity=25.8992805755396, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI70166599, Length=278, Percent_Identity=25.8992805755396, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI70166645, Length=278, Percent_Identity=25.8992805755396, Blast_Score=69, Evalue=6e-12,
Organism=Escherichia coli, GI1788657, Length=250, Percent_Identity=35.2, Blast_Score=130, Evalue=7e-32,
Organism=Drosophila melanogaster, GI116007752, Length=299, Percent_Identity=28.7625418060201, Blast_Score=74, Evalue=6e-14,
Organism=Drosophila melanogaster, GI281362146, Length=260, Percent_Identity=26.9230769230769, Blast_Score=65, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24648455, Length=260, Percent_Identity=26.9230769230769, Blast_Score=64, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 27714; Mature: 27714

Theoretical pI: Translated: 9.22; Mature: 9.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLVIEYEGTRYSGFQVQDKGATIQGELETAIRKLTSEQTRVSGSGRTDAGVHAAYQVVS
CEEEEEECCCEECCEEEECCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
YLSESSLGLDKIKGGLNFYLPPDIAVKEVHFVAESFDVRRRAISREYSYQILNSKTRSPL
HHHHCCCCHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
ARLQAWQVPYKLDIKLMEQAFKMLEGEHDFIAFASSLGPEIKKTVRRVNWTRLGIREDRL
HHHHHHCCCCEEHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHCHHCCCCCCCCE
IFNIEANSFLTHQVRNTIGTLVELGRGTMSMSEFEALFEAKTPGLAGPAAPACGLSLVRV
EEEEECCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEE
NYREPFGS
ECCCCCCC
>Mature Secondary Structure
MLLVIEYEGTRYSGFQVQDKGATIQGELETAIRKLTSEQTRVSGSGRTDAGVHAAYQVVS
CEEEEEECCCEECCEEEECCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
YLSESSLGLDKIKGGLNFYLPPDIAVKEVHFVAESFDVRRRAISREYSYQILNSKTRSPL
HHHHCCCCHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
ARLQAWQVPYKLDIKLMEQAFKMLEGEHDFIAFASSLGPEIKKTVRRVNWTRLGIREDRL
HHHHHHCCCCEEHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHCHHCCCCCCCCE
IFNIEANSFLTHQVRNTIGTLVELGRGTMSMSEFEALFEAKTPGLAGPAAPACGLSLVRV
EEEEECCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEE
NYREPFGS
ECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA