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The map label for this gene is mdh

Identifier: 57234730

GI number: 57234730

Start: 428007

End: 428930

Strand: Direct

Name: mdh

Synonym: DET0451

Alternate gene names: 57234730

Gene position: 428007-428930 (Clockwise)

Preceding gene: 57234731

Following gene: 57234729

Centisome position: 29.12

GC content: 54.87

Gene sequence:

>924_bases
ATGCCCAAAATTAGTGTCATAGGTGCCGGCAATGTGGGGGCAACACTTGCCCAGCGCCTTATAGAAAAAGACTTTGCAGA
TGTGGTCATGCTGGACGTGGTGGAGGGTATTCCCCAGGGTAAGGCTCTGGACATTTCCCAGTCTGCCAATGTACTTGGTT
TCAGCCATACCATTACCGGCAGCAATGATTATGCCGAAACAGCCGGCTCTGAAATAGTGGTGATAACCGCCGGTATTGCC
CGCAAACCGGGCATGACCCGTGAGGAACTGCTGGCTATAAACCAGAAGATTATGACCGATGTGGTTTCAAACTGCCTTAA
ATACTCCCCCGAAGCCACCTTGGTGGTCGTCTCCAACCCGGTGGATACCATGACCTATCTGGCCTGGAAACTTTCAGGCC
TGCCCCGGAAACGGGTGGTGGGTCTTTCAGGCGTGCTGGACGGAGGGCGTCTGGCTACCTTTGTAGCCCGTGAGCTTGGG
GTAAAACCTTCGGCTGTTACGCCCTGCGTAATGGGCGAACACGGCGGCAGCATGGTGGTCATGCCCCGTTTTACCCTGGT
AAGCGGCAAACCCCTGTCCGAACTGGTTTCGGCTGAAAAAGCAGATGAACTGGCCAAACGGGCAGTCAACGGCGGTGCCG
AGATAGTGGCTTTCCTTAAAACCGGCAGCGCTTTTTATGCCCCGTCTGCTTCCATAGCTGCCATGGCGGAGGCTATTTTT
ACGGGCAGCGGAAAGGTTATGAACTGTGCCGCGGTGCTGGATGGCGAATACGGCCTGAAAAATATTGTGCTGGGCGTGCC
GGTAAAGCTGGGCAAAGGCGGCCTACAGGAAATTATCACCTTGCCGCTGGATGGGACGGAAAACGCCCGCCTTTTAGCAT
CTGCCGAAGTAGTGAAAGGGCAGATAGCCGCCCTTTCGCTTTAA

Upstream 100 bases:

>100_bases
GCCATAATAGCCAAAATGAAATAAAAGTGCGGCTAGTCTTATGGGTATTGTACCCTCAGGCTTAGCTGCCTGAATTGATA
ACAGGTGAGGAAGAAAATAA

Downstream 100 bases:

>100_bases
TAAGTTTAAAGGAGACAATGCAGTGAGTAATGATAAAGAATTTCTGGATGCTTTTTTAAAGCTCAAAACCGAGCTGGATG
ATACGGTTTACAAGCTTGAA

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MPKISVIGAGNVGATLAQRLIEKDFADVVMLDVVEGIPQGKALDISQSANVLGFSHTITGSNDYAETAGSEIVVITAGIA
RKPGMTREELLAINQKIMTDVVSNCLKYSPEATLVVVSNPVDTMTYLAWKLSGLPRKRVVGLSGVLDGGRLATFVARELG
VKPSAVTPCVMGEHGGSMVVMPRFTLVSGKPLSELVSAEKADELAKRAVNGGAEIVAFLKTGSAFYAPSASIAAMAEAIF
TGSGKVMNCAAVLDGEYGLKNIVLGVPVKLGKGGLQEIITLPLDGTENARLLASAEVVKGQIAALSL

Sequences:

>Translated_307_residues
MPKISVIGAGNVGATLAQRLIEKDFADVVMLDVVEGIPQGKALDISQSANVLGFSHTITGSNDYAETAGSEIVVITAGIA
RKPGMTREELLAINQKIMTDVVSNCLKYSPEATLVVVSNPVDTMTYLAWKLSGLPRKRVVGLSGVLDGGRLATFVARELG
VKPSAVTPCVMGEHGGSMVVMPRFTLVSGKPLSELVSAEKADELAKRAVNGGAEIVAFLKTGSAFYAPSASIAAMAEAIF
TGSGKVMNCAAVLDGEYGLKNIVLGVPVKLGKGGLQEIITLPLDGTENARLLASAEVVKGQIAALSL
>Mature_306_residues
PKISVIGAGNVGATLAQRLIEKDFADVVMLDVVEGIPQGKALDISQSANVLGFSHTITGSNDYAETAGSEIVVITAGIAR
KPGMTREELLAINQKIMTDVVSNCLKYSPEATLVVVSNPVDTMTYLAWKLSGLPRKRVVGLSGVLDGGRLATFVARELGV
KPSAVTPCVMGEHGGSMVVMPRFTLVSGKPLSELVSAEKADELAKRAVNGGAEIVAFLKTGSAFYAPSASIAAMAEAIFT
GSGKVMNCAAVLDGEYGLKNIVLGVPVKLGKGGLQEIITLPLDGTENARLLASAEVVKGQIAALSL

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 3 family

Homologues:

Organism=Homo sapiens, GI5031857, Length=307, Percent_Identity=34.5276872964169, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI260099723, Length=307, Percent_Identity=34.5276872964169, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI47059044, Length=305, Percent_Identity=34.0983606557377, Blast_Score=172, Evalue=5e-43,
Organism=Homo sapiens, GI221136809, Length=305, Percent_Identity=34.0983606557377, Blast_Score=172, Evalue=5e-43,
Organism=Homo sapiens, GI291575128, Length=305, Percent_Identity=33.1147540983607, Blast_Score=166, Evalue=2e-41,
Organism=Homo sapiens, GI4557032, Length=305, Percent_Identity=33.1147540983607, Blast_Score=166, Evalue=2e-41,
Organism=Homo sapiens, GI9257228, Length=305, Percent_Identity=31.4754098360656, Blast_Score=155, Evalue=5e-38,
Organism=Homo sapiens, GI4504973, Length=305, Percent_Identity=31.4754098360656, Blast_Score=155, Evalue=5e-38,
Organism=Homo sapiens, GI15082234, Length=303, Percent_Identity=31.3531353135314, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI260099725, Length=178, Percent_Identity=39.8876404494382, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI260099727, Length=178, Percent_Identity=39.8876404494382, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI207028494, Length=193, Percent_Identity=33.160621761658, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI103472011, Length=308, Percent_Identity=27.9220779220779, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI21735621, Length=293, Percent_Identity=29.6928327645051, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI103472015, Length=208, Percent_Identity=28.8461538461538, Blast_Score=72, Evalue=7e-13,
Organism=Escherichia coli, GI1789632, Length=290, Percent_Identity=31.0344827586207, Blast_Score=95, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17535107, Length=292, Percent_Identity=33.2191780821918, Blast_Score=154, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI17554310, Length=291, Percent_Identity=32.3024054982818, Blast_Score=90, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6322765, Length=291, Percent_Identity=30.2405498281787, Blast_Score=82, Evalue=7e-17,
Organism=Drosophila melanogaster, GI17136226, Length=314, Percent_Identity=32.484076433121, Blast_Score=167, Evalue=6e-42,
Organism=Drosophila melanogaster, GI45550422, Length=317, Percent_Identity=30.5993690851735, Blast_Score=136, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24647881, Length=318, Percent_Identity=31.4465408805031, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24663595, Length=252, Percent_Identity=27.3809523809524, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24663599, Length=246, Percent_Identity=30.0813008130081, Blast_Score=68, Evalue=7e-12,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): MDH_DEHE1 (Q3Z9A4)

Other databases:

- EMBL:   CP000027
- RefSeq:   YP_181195.1
- ProteinModelPortal:   Q3Z9A4
- SMR:   Q3Z9A4
- STRING:   Q3Z9A4
- GeneID:   3230199
- GenomeReviews:   CP000027_GR
- KEGG:   det:DET0451
- NMPDR:   fig|243164.3.peg.713
- TIGR:   DET0451
- eggNOG:   COG0039
- HOGENOM:   HBG566126
- OMA:   YAVYKES
- PhylomeDB:   Q3Z9A4
- ProtClustDB:   PRK06223
- BioCyc:   DETH243164:DET_0451-MONOMER
- GO:   GO:0005488
- GO:   GO:0006096
- HAMAP:   MF_00487
- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR011275
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.90.110.10
- Gene3D:   G3DSA:3.40.50.720
- PIRSF:   PIRSF000102
- PRINTS:   PR00086
- TIGRFAMs:   TIGR01763

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N; SSF56327 Lactate_DH/Glyco_hydro_4_C

EC number: =1.1.1.37

Molecular weight: Translated: 31781; Mature: 31650

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: NA

Important sites: ACT_SITE 174-174 BINDING 32-32 BINDING 81-81 BINDING 87-87 BINDING 94-94 BINDING 119-119 BINDING 150-150

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKISVIGAGNVGATLAQRLIEKDFADVVMLDVVEGIPQGKALDISQSANVLGFSHTITG
CCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCEEEEEEEECC
SNDYAETAGSEIVVITAGIARKPGMTREELLAINQKIMTDVVSNCLKYSPEATLVVVSNP
CCCHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECC
VDTMTYLAWKLSGLPRKRVVGLSGVLDGGRLATFVARELGVKPSAVTPCVMGEHGGSMVV
HHHHHHHHHHHCCCCHHHEEEHHCCCCCCHHHHHHHHHHCCCCCCCCCEEECCCCCCEEE
MPRFTLVSGKPLSELVSAEKADELAKRAVNGGAEIVAFLKTGSAFYAPSASIAAMAEAIF
EEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCHHEEEEEECCCEEECCCHHHHHHHHHHH
TGSGKVMNCAAVLDGEYGLKNIVLGVPVKLGKGGLQEIITLPLDGTENARLLASAEVVKG
CCCCCEEEEEEEECCCCCCCCEEEECCCCCCCCCCHHHEEECCCCCCCCEEEEHHHHHHC
QIAALSL
CEEEECC
>Mature Secondary Structure 
PKISVIGAGNVGATLAQRLIEKDFADVVMLDVVEGIPQGKALDISQSANVLGFSHTITG
CCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCEEEEEEEECC
SNDYAETAGSEIVVITAGIARKPGMTREELLAINQKIMTDVVSNCLKYSPEATLVVVSNP
CCCHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECC
VDTMTYLAWKLSGLPRKRVVGLSGVLDGGRLATFVARELGVKPSAVTPCVMGEHGGSMVV
HHHHHHHHHHHCCCCHHHEEEHHCCCCCCHHHHHHHHHHCCCCCCCCCEEECCCCCCEEE
MPRFTLVSGKPLSELVSAEKADELAKRAVNGGAEIVAFLKTGSAFYAPSASIAAMAEAIF
EEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCHHEEEEEECCCEEECCCHHHHHHHHHHH
TGSGKVMNCAAVLDGEYGLKNIVLGVPVKLGKGGLQEIITLPLDGTENARLLASAEVVKG
CCCCCEEEEEEEECCCCCCCCEEEECCCCCCCCCCHHHEEECCCCCCCCEEEEHHHHHHC
QIAALSL
CEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA