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The map label for this gene is holB [H]

Identifier: 57234678

GI number: 57234678

Start: 488923

End: 489942

Strand: Direct

Name: holB [H]

Synonym: DET0535

Alternate gene names: 57234678

Gene position: 488923-489942 (Clockwise)

Preceding gene: 57234679

Following gene: 57234677

Centisome position: 33.27

GC content: 47.55

Gene sequence:

>1020_bases
ATGTGGCAGATAATAGGTCAGGACAAGGCAGTCTCCTTCCTCTGTAACAGCCTCAATCAGGACAAGCTTTGCCACGCCTA
TGTGTTTTACGGCCAGTCCGGGGTGGGTAAGAAACGGATGGCACTTAGTTTTGCTCAGGCGCTAAATTGTCAGGCTGCCT
CACCCCCATGTCAGGAATGCCCGGTGTGTGAGCGCATCATGGCCGGAAACTTTCCGGATGTTATCCATATAGAGCTTCTT
TCAGCCGAAGATTCCTCTGACGGCAAAGCTAAGGCCGAAATAGGGGTTAAACAGGTAGAGGATATCCAGCACGCCGCCAG
TTTGCCCCCGTTTGAGGGCAAGTACAAGGTTTTTATTATCCATCAGGCTGAAAAGCTGTCAGTAGAATCTTCAAACCGCC
TTCTGAAAACGCTGGAAGAGCCGGGTTCTCAAAATATCTTTATTTTGCTGACCGCTGATAAATCACTCCTGCTGCCCACG
GTTTTATCCCGTTGCCAGATTTTGGAATTAAAATATGCCGAAACCGGTCTTATCCATGAGGCATTTATTAAGCAAGGTTT
GGATGAAGAAAAAGCTGAACTTTTGTCTCACCTGAGTTATGGCCGGGTGGGCTGGGCTATGGAAGCAGCCTCAAACCCCG
AAATTTTGGAAGAACGTAAAGCTAAGATTGATAAGATTATGCGGGTACTGGGGCAGGACGAGGAAGACCGCTTTAATTAT
GCCGCTGATGTTTCAAATCTGTTTGGTAAAAACCGGGCAGAGGCTGAAGAGGTACTGGATATGTGGCTGGGGCTTTTTCG
GGATATGCTGCTACTTAAGGCTGATGCCGGCCAAAACATGACTAATATTGATTATAAAGATATACTATATCAGGCCTGTG
GCGGGCTTGGGCTTGTGGATATACGTGCGGGTATAGATAATATACTTAAGGCCCAAAGTCAGCTGCGGGCAAATGCCAAC
CCCAGGCTTTGTCTGGAAGTGCTTATGCTCAGTTTGCCCTTGGTAAAGGAAGGACTATAA

Upstream 100 bases:

>100_bases
CTGTAGTGATGCTGAAAAATGGTGAAGACCGCCTGATACGGCGGCGCGAAACTGTGGAAGACCTTAGCCGTCTGGATTCG
GTCTAAGCCGGGTTTCTTAT

Downstream 100 bases:

>100_bases
AAATGGTTGATATTGTAAAGATACGCTATAAACAAGCCGGCAAGATATATTATTTTGACCCTGCCGGTTTTGAACTTTAC
CCGTATGACTGTGTAGTGGT

Product: DNA polymerase III, delta prime subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MWQIIGQDKAVSFLCNSLNQDKLCHAYVFYGQSGVGKKRMALSFAQALNCQAASPPCQECPVCERIMAGNFPDVIHIELL
SAEDSSDGKAKAEIGVKQVEDIQHAASLPPFEGKYKVFIIHQAEKLSVESSNRLLKTLEEPGSQNIFILLTADKSLLLPT
VLSRCQILELKYAETGLIHEAFIKQGLDEEKAELLSHLSYGRVGWAMEAASNPEILEERKAKIDKIMRVLGQDEEDRFNY
AADVSNLFGKNRAEAEEVLDMWLGLFRDMLLLKADAGQNMTNIDYKDILYQACGGLGLVDIRAGIDNILKAQSQLRANAN
PRLCLEVLMLSLPLVKEGL

Sequences:

>Translated_339_residues
MWQIIGQDKAVSFLCNSLNQDKLCHAYVFYGQSGVGKKRMALSFAQALNCQAASPPCQECPVCERIMAGNFPDVIHIELL
SAEDSSDGKAKAEIGVKQVEDIQHAASLPPFEGKYKVFIIHQAEKLSVESSNRLLKTLEEPGSQNIFILLTADKSLLLPT
VLSRCQILELKYAETGLIHEAFIKQGLDEEKAELLSHLSYGRVGWAMEAASNPEILEERKAKIDKIMRVLGQDEEDRFNY
AADVSNLFGKNRAEAEEVLDMWLGLFRDMLLLKADAGQNMTNIDYKDILYQACGGLGLVDIRAGIDNILKAQSQLRANAN
PRLCLEVLMLSLPLVKEGL
>Mature_339_residues
MWQIIGQDKAVSFLCNSLNQDKLCHAYVFYGQSGVGKKRMALSFAQALNCQAASPPCQECPVCERIMAGNFPDVIHIELL
SAEDSSDGKAKAEIGVKQVEDIQHAASLPPFEGKYKVFIIHQAEKLSVESSNRLLKTLEEPGSQNIFILLTADKSLLLPT
VLSRCQILELKYAETGLIHEAFIKQGLDEEKAELLSHLSYGRVGWAMEAASNPEILEERKAKIDKIMRVLGQDEEDRFNY
AADVSNLFGKNRAEAEEVLDMWLGLFRDMLLLKADAGQNMTNIDYKDILYQACGGLGLVDIRAGIDNILKAQSQLRANAN
PRLCLEVLMLSLPLVKEGL

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786676, Length=170, Percent_Identity=37.0588235294118, Blast_Score=107, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004622 [H]

Pfam domain/function: NA

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 37615; Mature: 37615

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00675 SIGMA54_INTERACT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWQIIGQDKAVSFLCNSLNQDKLCHAYVFYGQSGVGKKRMALSFAQALNCQAASPPCQEC
CCEECCCHHHHHHHHHCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCC
PVCERIMAGNFPDVIHIELLSAEDSSDGKAKAEIGVKQVEDIQHAASLPPFEGKYKVFII
CHHHHHHCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEE
HQAEKLSVESSNRLLKTLEEPGSQNIFILLTADKSLLLPTVLSRCQILELKYAETGLIHE
ECHHHHCCCHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHEEEHHHHHHHHH
AFIKQGLDEEKAELLSHLSYGRVGWAMEAASNPEILEERKAKIDKIMRVLGQDEEDRFNY
HHHHCCCCHHHHHHHHHHCCCCCCEEHHCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHH
AADVSNLFGKNRAEAEEVLDMWLGLFRDMLLLKADAGQNMTNIDYKDILYQACGGLGLVD
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCEEH
IRAGIDNILKAQSQLRANANPRLCLEVLMLSLPLVKEGL
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MWQIIGQDKAVSFLCNSLNQDKLCHAYVFYGQSGVGKKRMALSFAQALNCQAASPPCQEC
CCEECCCHHHHHHHHHCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCC
PVCERIMAGNFPDVIHIELLSAEDSSDGKAKAEIGVKQVEDIQHAASLPPFEGKYKVFII
CHHHHHHCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEE
HQAEKLSVESSNRLLKTLEEPGSQNIFILLTADKSLLLPTVLSRCQILELKYAETGLIHE
ECHHHHCCCHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHEEEHHHHHHHHH
AFIKQGLDEEKAELLSHLSYGRVGWAMEAASNPEILEERKAKIDKIMRVLGQDEEDRFNY
HHHHCCCCHHHHHHHHHHCCCCCCEEHHCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHH
AADVSNLFGKNRAEAEEVLDMWLGLFRDMLLLKADAGQNMTNIDYKDILYQACGGLGLVD
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCEEH
IRAGIDNILKAQSQLRANANPRLCLEVLMLSLPLVKEGL
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]