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The map label for this gene is aspS

Identifier: 57234484

GI number: 57234484

Start: 654040

End: 655836

Strand: Direct

Name: aspS

Synonym: DET0708

Alternate gene names: 57234484

Gene position: 654040-655836 (Clockwise)

Preceding gene: 57234485

Following gene: 57234483

Centisome position: 44.5

GC content: 50.31

Gene sequence:

>1797_bases
ATGCTTAAAACTCATAGCTGTGCCTTAACTCAGGAAAATATTGGCACTGAAGTTACTCTGGCAGGTTGGGTACACCGCCG
CCGAGACCATGGCGGGGTTATATTTATTGACCTTCGTGACCGGGAAGGCATTGTTCAGGTGGTATTTAACCCCGAACAGT
CCGCTGCCTGTCTGGATATCGGTAAAGAACTGCGGAGTGAATATGTCCTGCAGGTAAAAGGCACTGTCAGCCACCGCCCG
GCCGGTACGGAAAACAGCCGTATGCTCTCCGGTTTGGTGGAAGTGGTGGCGGAAGGTGCCAAAATCTTAAATACAGCCAA
AACCCCGCCCTTTTATATTAACGAAGAGGTAGAGGTGGACGAGAGCTTAAGGCTCAAGTACCGCTATCTGGATATACGCC
GCCAGGGCATGAAAAACAACCTGATTATCCGCCATAAAACTGTCAGCTTTATGAGGCAGTTTTTAAATGACCGGGGTTTT
ATAGAGATTGAAACCCCCATTCTGATTAAAAGCACGCCTGAGGGGGCGCGTGACTATCTGGTTCCCTCCCGCTTGTTTCC
GGGGCAGTTTTTTGCCTTGCCCCAGTCTCCCCAGCAGTTAAAACAGCTGCTTATGGTGGCGGGTATGGAAAAATACTATC
AGGTAGCCCGCTGTTTCCGTGATGAAGACTTAAGGGCAGACCGCCAGCCGGAATTTACCCAGCTTGATATGGAAATGAGC
TTTGTTGACGAAAATGATATGATGCAGCTCATGGAAGACCTGTTTACCGGGCTGGTAGCCAGTGTCCGCCCGGATATGAA
ATATAATAAAAAGTTTCCCCGTATCAGCTTTGCTGATGCTATGGAGAAATACGGCTGTGACAAGCCTGACCTTCGCTTCG
GTATGGAACTGGCAGATATTACCGATATCGGCGCTTCTTCGGCTTTCGGTGTTTTTAAAAATGTGGCTGCCCAGGGCGGA
GCTATCAAAGCCATTTCCGCTCCCGGTTGCGGCGGGTACAACAAGAGCCAGCAGGAAGAACTGATAAATCTGGCTAAGAA
GTACGGGGCGGCCGGTTTGGTACCCATTTCACTGGGTGCGGAAAGCGGGGAGCTTAAAGATTTGACTATGGAAATGGTTA
AATCGGTATCTGCCAAGTACCTTACTCTGGAAGAAATCAAAACCATAGCCGAACGCAGCGGGGCTAAGCCGGGTGGCCTT
ATTTTGATTGTGGCCGGTGCCCGTAAAATGGTGAACAGCGTACTGGGTGAAATGAGAAACCATCTGGCTGCAAAGCTTGA
TTTATGTGATAAAAATGAACTCAGTTTTGCCTTTGTGGTAGATTTCCCTCTCTTCCAGTGGGATGAGGAAGGCAAACGCT
GGGATTCGGTTCATCACCCCTTTACCGCTCCGCTTGAGTCTGACCTGCCCCTTATGGATACCGACCCCGCCAGGGTAGGC
AGCCGGGCTTATGACGTGGTTTGCAACGGCTATGAGATTGCCGGCGGCAGTATCAGAATCCACCAGGCAGACCTGCAGAG
GAAAGTATTCCATCTGCTGGGGTATAATAACGAGCAGATAGATGAACGCTTCGGCCACCTGCTGGAAGCTTTTGAATTCG
GGGCTCCCCCCCATGGAGGTGTTGCCCCCGGTATAGACCGCTTTGTAATGCTGCTGGCAGGGGAAACTTCCATACGTGAA
GTCATACCCTTCCCCAAGAATCAGGCCGCTCAGGACCTTCTGTTTGGGGCTCCGTCAGTGGTAGATGACAAGCAAATAAG
GGATTTACATATCCGTATTCAAACGGAAAAAGAATAG

Upstream 100 bases:

>100_bases
CTGGGCGAATACGAAAAAAAATAGCTTTCAGCGTTTGAACAAACACAATGTATAGGATATAATCTTTTCTAATTGTCTTG
ACTAAATTTAGAAGGTGCGC

Downstream 100 bases:

>100_bases
TTTTTTAGATTGAGGTGCCATGAAAGTAACAGATAAAAAGATAGAGGGCTGCCAGGCCAGCATAACGGTAGAGATGGATC
CGGCTGAAGTTGAGGCGGGT

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS

Number of amino acids: Translated: 598; Mature: 598

Protein sequence:

>598_residues
MLKTHSCALTQENIGTEVTLAGWVHRRRDHGGVIFIDLRDREGIVQVVFNPEQSAACLDIGKELRSEYVLQVKGTVSHRP
AGTENSRMLSGLVEVVAEGAKILNTAKTPPFYINEEVEVDESLRLKYRYLDIRRQGMKNNLIIRHKTVSFMRQFLNDRGF
IEIETPILIKSTPEGARDYLVPSRLFPGQFFALPQSPQQLKQLLMVAGMEKYYQVARCFRDEDLRADRQPEFTQLDMEMS
FVDENDMMQLMEDLFTGLVASVRPDMKYNKKFPRISFADAMEKYGCDKPDLRFGMELADITDIGASSAFGVFKNVAAQGG
AIKAISAPGCGGYNKSQQEELINLAKKYGAAGLVPISLGAESGELKDLTMEMVKSVSAKYLTLEEIKTIAERSGAKPGGL
ILIVAGARKMVNSVLGEMRNHLAAKLDLCDKNELSFAFVVDFPLFQWDEEGKRWDSVHHPFTAPLESDLPLMDTDPARVG
SRAYDVVCNGYEIAGGSIRIHQADLQRKVFHLLGYNNEQIDERFGHLLEAFEFGAPPHGGVAPGIDRFVMLLAGETSIRE
VIPFPKNQAAQDLLFGAPSVVDDKQIRDLHIRIQTEKE

Sequences:

>Translated_598_residues
MLKTHSCALTQENIGTEVTLAGWVHRRRDHGGVIFIDLRDREGIVQVVFNPEQSAACLDIGKELRSEYVLQVKGTVSHRP
AGTENSRMLSGLVEVVAEGAKILNTAKTPPFYINEEVEVDESLRLKYRYLDIRRQGMKNNLIIRHKTVSFMRQFLNDRGF
IEIETPILIKSTPEGARDYLVPSRLFPGQFFALPQSPQQLKQLLMVAGMEKYYQVARCFRDEDLRADRQPEFTQLDMEMS
FVDENDMMQLMEDLFTGLVASVRPDMKYNKKFPRISFADAMEKYGCDKPDLRFGMELADITDIGASSAFGVFKNVAAQGG
AIKAISAPGCGGYNKSQQEELINLAKKYGAAGLVPISLGAESGELKDLTMEMVKSVSAKYLTLEEIKTIAERSGAKPGGL
ILIVAGARKMVNSVLGEMRNHLAAKLDLCDKNELSFAFVVDFPLFQWDEEGKRWDSVHHPFTAPLESDLPLMDTDPARVG
SRAYDVVCNGYEIAGGSIRIHQADLQRKVFHLLGYNNEQIDERFGHLLEAFEFGAPPHGGVAPGIDRFVMLLAGETSIRE
VIPFPKNQAAQDLLFGAPSVVDDKQIRDLHIRIQTEKE
>Mature_598_residues
MLKTHSCALTQENIGTEVTLAGWVHRRRDHGGVIFIDLRDREGIVQVVFNPEQSAACLDIGKELRSEYVLQVKGTVSHRP
AGTENSRMLSGLVEVVAEGAKILNTAKTPPFYINEEVEVDESLRLKYRYLDIRRQGMKNNLIIRHKTVSFMRQFLNDRGF
IEIETPILIKSTPEGARDYLVPSRLFPGQFFALPQSPQQLKQLLMVAGMEKYYQVARCFRDEDLRADRQPEFTQLDMEMS
FVDENDMMQLMEDLFTGLVASVRPDMKYNKKFPRISFADAMEKYGCDKPDLRFGMELADITDIGASSAFGVFKNVAAQGG
AIKAISAPGCGGYNKSQQEELINLAKKYGAAGLVPISLGAESGELKDLTMEMVKSVSAKYLTLEEIKTIAERSGAKPGGL
ILIVAGARKMVNSVLGEMRNHLAAKLDLCDKNELSFAFVVDFPLFQWDEEGKRWDSVHHPFTAPLESDLPLMDTDPARVG
SRAYDVVCNGYEIAGGSIRIHQADLQRKVFHLLGYNNEQIDERFGHLLEAFEFGAPPHGGVAPGIDRFVMLLAGETSIRE
VIPFPKNQAAQDLLFGAPSVVDDKQIRDLHIRIQTEKE

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI40789249, Length=606, Percent_Identity=42.2442244224422, Blast_Score=447, Evalue=1e-125,
Organism=Homo sapiens, GI5031815, Length=296, Percent_Identity=26.3513513513513, Blast_Score=97, Evalue=6e-20,
Organism=Homo sapiens, GI194272210, Length=296, Percent_Identity=26.3513513513513, Blast_Score=96, Evalue=7e-20,
Organism=Homo sapiens, GI4758762, Length=288, Percent_Identity=26.7361111111111, Blast_Score=81, Evalue=4e-15,
Organism=Homo sapiens, GI226491502, Length=279, Percent_Identity=27.5985663082437, Blast_Score=77, Evalue=5e-14,
Organism=Homo sapiens, GI45439306, Length=287, Percent_Identity=25.4355400696864, Blast_Score=76, Evalue=8e-14,
Organism=Escherichia coli, GI1788173, Length=600, Percent_Identity=44.8333333333333, Blast_Score=502, Evalue=1e-143,
Organism=Escherichia coli, GI1790571, Length=316, Percent_Identity=25, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI1789256, Length=324, Percent_Identity=24.6913580246914, Blast_Score=89, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI32566633, Length=602, Percent_Identity=34.21926910299, Blast_Score=315, Evalue=5e-86,
Organism=Caenorhabditis elegans, GI17535925, Length=312, Percent_Identity=25.9615384615385, Blast_Score=105, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI71994340, Length=312, Percent_Identity=25.9615384615385, Blast_Score=105, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI17535927, Length=312, Percent_Identity=25.9615384615385, Blast_Score=105, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI17551876, Length=267, Percent_Identity=26.5917602996255, Blast_Score=72, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6325153, Length=638, Percent_Identity=32.9153605015674, Blast_Score=273, Evalue=6e-74,
Organism=Saccharomyces cerevisiae, GI6321807, Length=325, Percent_Identity=24, Blast_Score=84, Evalue=9e-17,
Organism=Saccharomyces cerevisiae, GI6324256, Length=164, Percent_Identity=27.4390243902439, Blast_Score=80, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6320242, Length=296, Percent_Identity=22.6351351351351, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323011, Length=264, Percent_Identity=26.8939393939394, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24584738, Length=611, Percent_Identity=37.315875613748, Blast_Score=367, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24640849, Length=264, Percent_Identity=28.4090909090909, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24640851, Length=264, Percent_Identity=28.4090909090909, Blast_Score=97, Evalue=4e-20,
Organism=Drosophila melanogaster, GI17136276, Length=274, Percent_Identity=26.6423357664234, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI19921528, Length=292, Percent_Identity=26.027397260274, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYD_DEHE1 (Q3Z8K0)

Other databases:

- EMBL:   CP000027
- RefSeq:   YP_181449.1
- ProteinModelPortal:   Q3Z8K0
- SMR:   Q3Z8K0
- STRING:   Q3Z8K0
- GeneID:   3229951
- GenomeReviews:   CP000027_GR
- KEGG:   det:DET0708
- NMPDR:   fig|243164.3.peg.815
- TIGR:   DET0708
- eggNOG:   COG0173
- HOGENOM:   HBG396032
- OMA:   AFPKTQQ
- PhylomeDB:   Q3Z8K0
- ProtClustDB:   PRK00476
- BioCyc:   DETH243164:DET_0708-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00044_B
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:3.30.1360.30
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF5
- PRINTS:   PR01042
- TIGRFAMs:   TIGR00459

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.12

Molecular weight: Translated: 66856; Mature: 66856

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKTHSCALTQENIGTEVTLAGWVHRRRDHGGVIFIDLRDREGIVQVVFNPEQSAACLDI
CCCCCCCCCCHHCCCCEEEEHHHHHHHCCCCCEEEEEEECCCCEEEEEECCCCCHHHHHH
GKELRSEYVLQVKGTVSHRPAGTENSRMLSGLVEVVAEGAKILNTAKTPPFYINEEVEVD
HHHHHHCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHCCCCCCEEECCCCCCC
ESLRLKYRYLDIRRQGMKNNLIIRHKTVSFMRQFLNDRGFIEIETPILIKSTPEGARDYL
HHHHEEHEEHHHHHCCCCCCEEEEEHHHHHHHHHHCCCCEEEECCCEEEECCCCCCHHCC
VPSRLFPGQFFALPQSPQQLKQLLMVAGMEKYYQVARCFRDEDLRADRQPEFTQLDMEMS
CCCCCCCCCEEECCCCHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCCEEECEEEE
FVDENDMMQLMEDLFTGLVASVRPDMKYNKKFPRISFADAMEKYGCDKPDLRFGMELADI
ECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHCCCHHHH
TDIGASSAFGVFKNVAAQGGAIKAISAPGCGGYNKSQQEELINLAKKYGAAGLVPISLGA
HHCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCC
ESGELKDLTMEMVKSVSAKYLTLEEIKTIAERSGAKPGGLILIVAGARKMVNSVLGEMRN
CCCCHHHHHHHHHHHHHHHEEEHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHH
HLAAKLDLCDKNELSFAFVVDFPLFQWDEEGKRWDSVHHPFTAPLESDLPLMDTDPARVG
HHHHHHCCCCCCCCCEEEEEECCCEEECCCCCHHHHCCCCCCCCCCCCCCCCCCCHHHHC
SRAYDVVCNGYEIAGGSIRIHQADLQRKVFHLLGYNNEQIDERFGHLLEAFEFGAPPHGG
CCEEEEEECCEEECCCEEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCC
VAPGIDRFVMLLAGETSIREVIPFPKNQAAQDLLFGAPSVVDDKQIRDLHIRIQTEKE
CCCCHHHHHHHHCCCCHHHHHCCCCCCHHHHHHHCCCCCCCCCCCEEEEEEEEEECCC
>Mature Secondary Structure
MLKTHSCALTQENIGTEVTLAGWVHRRRDHGGVIFIDLRDREGIVQVVFNPEQSAACLDI
CCCCCCCCCCHHCCCCEEEEHHHHHHHCCCCCEEEEEEECCCCEEEEEECCCCCHHHHHH
GKELRSEYVLQVKGTVSHRPAGTENSRMLSGLVEVVAEGAKILNTAKTPPFYINEEVEVD
HHHHHHCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHCCCCCCEEECCCCCCC
ESLRLKYRYLDIRRQGMKNNLIIRHKTVSFMRQFLNDRGFIEIETPILIKSTPEGARDYL
HHHHEEHEEHHHHHCCCCCCEEEEEHHHHHHHHHHCCCCEEEECCCEEEECCCCCCHHCC
VPSRLFPGQFFALPQSPQQLKQLLMVAGMEKYYQVARCFRDEDLRADRQPEFTQLDMEMS
CCCCCCCCCEEECCCCHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCCEEECEEEE
FVDENDMMQLMEDLFTGLVASVRPDMKYNKKFPRISFADAMEKYGCDKPDLRFGMELADI
ECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHCCCHHHH
TDIGASSAFGVFKNVAAQGGAIKAISAPGCGGYNKSQQEELINLAKKYGAAGLVPISLGA
HHCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCC
ESGELKDLTMEMVKSVSAKYLTLEEIKTIAERSGAKPGGLILIVAGARKMVNSVLGEMRN
CCCCHHHHHHHHHHHHHHHEEEHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHH
HLAAKLDLCDKNELSFAFVVDFPLFQWDEEGKRWDSVHHPFTAPLESDLPLMDTDPARVG
HHHHHHCCCCCCCCCEEEEEECCCEEECCCCCHHHHCCCCCCCCCCCCCCCCCCCHHHHC
SRAYDVVCNGYEIAGGSIRIHQADLQRKVFHLLGYNNEQIDERFGHLLEAFEFGAPPHGG
CCEEEEEECCEEECCCEEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCC
VAPGIDRFVMLLAGETSIREVIPFPKNQAAQDLLFGAPSVVDDKQIRDLHIRIQTEKE
CCCCHHHHHHHHCCCCHHHHHCCCCCCHHHHHHHCCCCCCCCCCCEEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA