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The map label for this gene is ndhI [H]

Identifier: 57234302

GI number: 57234302

Start: 849220

End: 849729

Strand: Direct

Name: ndhI [H]

Synonym: DET0928

Alternate gene names: 57234302

Gene position: 849220-849729 (Clockwise)

Preceding gene: 57234303

Following gene: 57234301

Centisome position: 57.78

GC content: 51.37

Gene sequence:

>510_bases
ATGCGCCTTACCTTCAAGCATCTTTTCCGCCCTTGGATAACTGTCCAGTATCCGGAAGAAAAACTGACTATGTCAAAAAG
GATACGGGGAACGCAGGTTATCTGGGTGAAGGAGACCTGTATTGCCTGCCTGGCCTGTGCCAGAGCCTGTCCGGTAAAGG
CCATCAATATGGAGGTTTCCCGCGGCGAAGACCGCAAACTTAAGGTAGACCATATGTCTATAGATTTCGGGCTGTGCGTC
TTTTGCGGTCTGTGTGTTGAATCCTGCCCTACCAAAACTTCAATTTACATGGGCTACGGCTACGAAACTACCACCTACCG
CTGTACCAACGTTGAAAAAGCGGAAGGGCAAAGCCGTTCCGAGTGCCGCTGCCGTGAGCTGATACTTACCGGGGATGAAC
TTGCCCCCTCGGCAACCCGGGTACTCAGCGGGTATGACCGCCCGGATGCCGCAGAAAAACTGGCTGAACAAACCCTGCTT
ATAAATAAGAAAGGCTACTTTGAAAGGTAA

Upstream 100 bases:

>100_bases
GCGGTAATGTTCGGGCTTATCCTCCTGTTCAGCCGTTTTTACAAGCTGGGAGGTGGACGTGTCAGCGTTAAGTAATACCG
GGGGCGGCATTCTTAAAGGA

Downstream 100 bases:

>100_bases
ACCGGGGTGGAAATAGCGTTCTGGATTATAGCCATAACCTGTATAGCCTCCGCCTTGCTGGTGGTGGGAGTGAAAAATAT
TTTCCACTCATCCCTCTGGC

Product: proton-translocating NADH-quinone oxidoreductase, I subunit

Products: NAD+; ubiquinol [C]

Alternate protein names: NAD(P)H dehydrogenase I subunit I; NDH-1 subunit I; NDH-I [H]

Number of amino acids: Translated: 169; Mature: 169

Protein sequence:

>169_residues
MRLTFKHLFRPWITVQYPEEKLTMSKRIRGTQVIWVKETCIACLACARACPVKAINMEVSRGEDRKLKVDHMSIDFGLCV
FCGLCVESCPTKTSIYMGYGYETTTYRCTNVEKAEGQSRSECRCRELILTGDELAPSATRVLSGYDRPDAAEKLAEQTLL
INKKGYFER

Sequences:

>Translated_169_residues
MRLTFKHLFRPWITVQYPEEKLTMSKRIRGTQVIWVKETCIACLACARACPVKAINMEVSRGEDRKLKVDHMSIDFGLCV
FCGLCVESCPTKTSIYMGYGYETTTYRCTNVEKAEGQSRSECRCRELILTGDELAPSATRVLSGYDRPDAAEKLAEQTLL
INKKGYFER
>Mature_169_residues
MRLTFKHLFRPWITVQYPEEKLTMSKRIRGTQVIWVKETCIACLACARACPVKAINMEVSRGEDRKLKVDHMSIDFGLCV
FCGLCVESCPTKTSIYMGYGYETTTYRCTNVEKAEGQSRSECRCRELILTGDELAPSATRVLSGYDRPDAAEKLAEQTLL
INKKGYFER

Specific function: NDH-1 shuttles electrons from an unknown electron donor, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory and/or the photosynthetic chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone

COG id: COG1143

COG function: function code C; Formate hydrogenlyase subunit 6/NADH:ubiquinone oxidoreductase 23 kD subunit (chain I)

Gene ontology:

Cell location: Cellular thylakoid membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Homo sapiens, GI4505371, Length=107, Percent_Identity=35.5140186915888, Blast_Score=70, Evalue=6e-13,
Organism=Escherichia coli, GI1788617, Length=105, Percent_Identity=37.1428571428571, Blast_Score=70, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17555194, Length=103, Percent_Identity=35.9223300970874, Blast_Score=68, Evalue=3e-12,
Organism=Drosophila melanogaster, GI17864306, Length=107, Percent_Identity=34.5794392523364, Blast_Score=71, Evalue=3e-13,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR012285
- InterPro:   IPR004497
- InterPro:   IPR010226 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: 1.6.99.5 [C]

Molecular weight: Translated: 19269; Mature: 19269

Theoretical pI: Translated: 8.34; Mature: 8.34

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

6.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
9.5 %Cys+Met (Translated Protein)
6.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
9.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLTFKHLFRPWITVQYPEEKLTMSKRIRGTQVIWVKETCIACLACARACPVKAINMEVS
CCCCHHHHCCCCEEEECCHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHCCCEEEEEEEEC
RGEDRKLKVDHMSIDFGLCVFCGLCVESCPTKTSIYMGYGYETTTYRCTNVEKAEGQSRS
CCCCCEEEEEEEECHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEEEECCCHHHCCCCCH
ECRCRELILTGDELAPSATRVLSGYDRPDAAEKLAEQTLLINKKGYFER
HHHHHHHEEECCCCCCHHHHHHCCCCCCHHHHHHHHHHEEECCCCCCCC
>Mature Secondary Structure
MRLTFKHLFRPWITVQYPEEKLTMSKRIRGTQVIWVKETCIACLACARACPVKAINMEVS
CCCCHHHHCCCCEEEECCHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHCCCEEEEEEEEC
RGEDRKLKVDHMSIDFGLCVFCGLCVESCPTKTSIYMGYGYETTTYRCTNVEKAEGQSRS
CCCCCEEEEEEEECHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEEEECCCHHHCCCCCH
ECRCRELILTGDELAPSATRVLSGYDRPDAAEKLAEQTLLINKKGYFER
HHHHHHHEEECCCCCCHHHHHHCCCCCCHHHHHHHHHHEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; ubiquinone [C]

Specific reaction: NADH + ubiquinone = NAD+ + ubiquinol [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA