Click here to switch to the map view.

The map label for this gene is dnaN [H]

Identifier: 57234032

GI number: 57234032

Start: 1125734

End: 1126870

Strand: Direct

Name: dnaN [H]

Synonym: DET1227

Alternate gene names: 57234032

Gene position: 1125734-1126870 (Clockwise)

Preceding gene: 57234033

Following gene: 57234030

Centisome position: 76.6

GC content: 50.57

Gene sequence:

>1137_bases
ATGAAACTTACATGCTTACAGGAAAATCTGAACAAGGGTCTAAACATTGTGGGCAAAGCGGCTGCCGGCCGGACTACCCT
GCCCATTACCAACAATGTCCTTATCTCAACTGATGACGGACGTTTAAAACTAGCCGCCACCAATCTCGAAATGGCTATCA
GCTGCTGGATAGGAGCCAAGATAGAGGAAGAAGGGAGCACCACCGTACCTGCCAAACTCCTGACCGAATTTGTCAGCTCT
CTGCCCAATGATAAAATAGAATTAAACCTTAATGCCAAGTCAAAGGCACTCAATATCAAATGCGCCCGCTTTGAGGCACG
CATAACAGGCGTTGACGCAAAAGACTTCCCGCCTGTACCCAAAGTGGAAAACGGCATCTCCACCAAGATTAACGTTGAGG
CCTTCCGCCAGGCCGTAAGCGAAGTGGTATTTGCTTCGGCAACTGACGAATCCCGCCCGGTGCTCACTGGTGTCAACGCC
GAATTTGAAGGCAGCACCCTAACTCTGGCTGCAGCTGACGGTTTCCGTTTGGCTGTCTATAAACTGCCTCTGCTGGAAGC
GGTAAAAACCGCTACTAAAGTAATCATTCCAGCCCGTACACTGGGTGAGCTTTCCCGCCTGGCCAGCATGGACGAGGAAG
AAGCCCTGATGATAAATGTAGATACTGCCAAGAGCCAGATACTTTTCAGGCTTAAAAATATTGAGTTGGTATCTCAGCTG
GTACAGGGAAATTTCCCCCAGTACAACCAGATAATCCCCCAAAGCTTTACTACCAAAGTGGTAGTAGATGCCAACCAGTT
CCTTATGGCCACCAGAACGGCTACAATCTTTGCCCGTGACGGCGGCGGCATAGTCCGGCTGATGATGAACCCCGGAGGCA
ATAATACCCCCGGTAAGCTCACCATCTCCGCCCGCTCTGAAGAAGTGGGTGACAATACCGGTGAACTTGATGCCGTAGTT
CAGGGTGAGGAAGCTAAAATAGCCTTCAACGGCAAATATCTTCTGGACGTCCTGGGAGTACTTAAAGCCCAGCAAGTATC
ACTGGAAGTAACCAGCCCGTCAAGCCCCGGTGTGATAAAACCGGTGGGGGCGGACAACTACATCCACGTTATTATGCCCA
TGTTCGTGCAGTGGTGA

Upstream 100 bases:

>100_bases
ATAAACTATTTACTGCCTCATTTTGAGCACAAAAATATCTTGTTTAAGTGCTATTCTTGGGATATACTGAAAGACCAGTA
AATAACCCCGGGGGGTAAAT

Downstream 100 bases:

>100_bases
GTACGTTCAAATCAAGGTAACCTTTGATGCTTACACCTTCAGGAGTAGCTGAAATTTTCAAATCCAGATAGGTGTAAGCA
TCTTTCTTTTCAGCGTTTGA

Product: DNA polymerase III, beta subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MKLTCLQENLNKGLNIVGKAAAGRTTLPITNNVLISTDDGRLKLAATNLEMAISCWIGAKIEEEGSTTVPAKLLTEFVSS
LPNDKIELNLNAKSKALNIKCARFEARITGVDAKDFPPVPKVENGISTKINVEAFRQAVSEVVFASATDESRPVLTGVNA
EFEGSTLTLAAADGFRLAVYKLPLLEAVKTATKVIIPARTLGELSRLASMDEEEALMINVDTAKSQILFRLKNIELVSQL
VQGNFPQYNQIIPQSFTTKVVVDANQFLMATRTATIFARDGGGIVRLMMNPGGNNTPGKLTISARSEEVGDNTGELDAVV
QGEEAKIAFNGKYLLDVLGVLKAQQVSLEVTSPSSPGVIKPVGADNYIHVIMPMFVQW

Sequences:

>Translated_378_residues
MKLTCLQENLNKGLNIVGKAAAGRTTLPITNNVLISTDDGRLKLAATNLEMAISCWIGAKIEEEGSTTVPAKLLTEFVSS
LPNDKIELNLNAKSKALNIKCARFEARITGVDAKDFPPVPKVENGISTKINVEAFRQAVSEVVFASATDESRPVLTGVNA
EFEGSTLTLAAADGFRLAVYKLPLLEAVKTATKVIIPARTLGELSRLASMDEEEALMINVDTAKSQILFRLKNIELVSQL
VQGNFPQYNQIIPQSFTTKVVVDANQFLMATRTATIFARDGGGIVRLMMNPGGNNTPGKLTISARSEEVGDNTGELDAVV
QGEEAKIAFNGKYLLDVLGVLKAQQVSLEVTSPSSPGVIKPVGADNYIHVIMPMFVQW
>Mature_378_residues
MKLTCLQENLNKGLNIVGKAAAGRTTLPITNNVLISTDDGRLKLAATNLEMAISCWIGAKIEEEGSTTVPAKLLTEFVSS
LPNDKIELNLNAKSKALNIKCARFEARITGVDAKDFPPVPKVENGISTKINVEAFRQAVSEVVFASATDESRPVLTGVNA
EFEGSTLTLAAADGFRLAVYKLPLLEAVKTATKVIIPARTLGELSRLASMDEEEALMINVDTAKSQILFRLKNIELVSQL
VQGNFPQYNQIIPQSFTTKVVVDANQFLMATRTATIFARDGGGIVRLMMNPGGNNTPGKLTISARSEEVGDNTGELDAVV
QGEEAKIAFNGKYLLDVLGVLKAQQVSLEVTSPSSPGVIKPVGADNYIHVIMPMFVQW

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=375, Percent_Identity=27.7333333333333, Blast_Score=141, Evalue=6e-35,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 40676; Mature: 40676

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLTCLQENLNKGLNIVGKAAAGRTTLPITNNVLISTDDGRLKLAATNLEMAISCWIGAK
CEEEEEHHHHCCCCEEEEECCCCCEECEEECCEEEECCCCEEEEEEECEEEEEEEEECCE
IEEEGSTTVPAKLLTEFVSSLPNDKIELNLNAKSKALNIKCARFEARITGVDAKDFPPVP
ECCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCEEEEEEEEEEEEEEECCCCCCCCCCC
KVENGISTKINVEAFRQAVSEVVFASATDESRPVLTGVNAEFEGSTLTLAAADGFRLAVY
CCCCCCCCEEEHHHHHHHHHHHEEEECCCCCCCEEEECCCEECCCEEEEEECCCEEEEEE
KLPLLEAVKTATKVIIPARTLGELSRLASMDEEEALMINVDTAKSQILFRLKNIELVSQL
ECHHHHHHHHHHEEEECCHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEEHHHHHHHHH
VQGNFPQYNQIIPQSFTTKVVVDANQFLMATRTATIFARDGGGIVRLMMNPGGNNTPGKL
HHCCCCCHHHCCCCCCCEEEEEECCCEEEEEEEEEEEEECCCCEEEEEECCCCCCCCCEE
TISARSEEVGDNTGELDAVVQGEEAKIAFNGKYLLDVLGVLKAQQVSLEVTSPSSPGVIK
EEEECCCCCCCCCCCEEEEEECCCEEEEECCCHHHHHHHHHCEEEEEEEEECCCCCCEEE
PVGADNYIHVIMPMFVQW
ECCCCCEEEEEEEHHHCC
>Mature Secondary Structure
MKLTCLQENLNKGLNIVGKAAAGRTTLPITNNVLISTDDGRLKLAATNLEMAISCWIGAK
CEEEEEHHHHCCCCEEEEECCCCCEECEEECCEEEECCCCEEEEEEECEEEEEEEEECCE
IEEEGSTTVPAKLLTEFVSSLPNDKIELNLNAKSKALNIKCARFEARITGVDAKDFPPVP
ECCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCEEEEEEEEEEEEEEECCCCCCCCCCC
KVENGISTKINVEAFRQAVSEVVFASATDESRPVLTGVNAEFEGSTLTLAAADGFRLAVY
CCCCCCCCEEEHHHHHHHHHHHEEEECCCCCCCEEEECCCEECCCEEEEEECCCEEEEEE
KLPLLEAVKTATKVIIPARTLGELSRLASMDEEEALMINVDTAKSQILFRLKNIELVSQL
ECHHHHHHHHHHEEEECCHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEEHHHHHHHHH
VQGNFPQYNQIIPQSFTTKVVVDANQFLMATRTATIFARDGGGIVRLMMNPGGNNTPGKL
HHCCCCCHHHCCCCCCCEEEEEECCCEEEEEEEEEEEEECCCCEEEEEECCCCCCCCCEE
TISARSEEVGDNTGELDAVVQGEEAKIAFNGKYLLDVLGVLKAQQVSLEVTSPSSPGVIK
EEEECCCCCCCCCCCEEEEEECCCEEEEECCCHHHHHHHHHCEEEEEEEEECCCCCCEEE
PVGADNYIHVIMPMFVQW
ECCCCCEEEEEEEHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]