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The map label for this gene is proB

Identifier: 57233928

GI number: 57233928

Start: 1170193

End: 1171314

Strand: Direct

Name: proB

Synonym: DET1282

Alternate gene names: 57233928

Gene position: 1170193-1171314 (Clockwise)

Preceding gene: 57233931

Following gene: 57233920

Centisome position: 79.62

GC content: 52.23

Gene sequence:

>1122_bases
ATGAACGGAAATTGTTACAAACGGATTGTAATAAAGCTGGGGACAAGCCTGCTGACCGGAGGCACAGGCAAGCTTGACCA
TGAGCGCATGGCGGATTTATGCCGCCAGATAGCTGACCTGACCCGTTTGGGTACGGAGGTAATTATTGTTTCCTCCGGGG
CGATTGCCGCCGGACGGGCTAAAATGGGTCTCCGGCATATCCCGAAAGATGTGCCTTTCAAGCAGGTGCTGGCGGCTATA
GGCCAGAGCCAGCTTATGAATTATTATGACCAGCTGTTCAGCCCCCACGGGCTGACTGTTGCCCAGGGTCTGCTGACCAA
GAGTGATTTGTCTGACCGTTCCGGTTACTTAAACGCCAGAAATACCCTGCTGGCTTTGATGGAGCTGGGAGTTATAACTA
TTGTCAATGAAAATGACGTGGTGGCGGTAGATGAAATCCAGCAGGCCAAATTCGGGGACAATGACAACCTGTCTGCCATG
GTAGCCAACCTGATAGAGGCTGATTTGCTGCTGATACTGACTAATATCCGCGGGCTTTATACAGCTGACCCCACCCTCCA
CCCGGATGCCCGGCTGATAACCGAAGTTAAAGAGATTACCGAAGAGCTGGAGAAACTGGCTGCCGGCAGTTCCAACAAGC
TGGGTACCGGCGGCATGGTTACCAAGCTGGAAGCCGCCAGGCTGGCAACATCTTCAGGGGTAAATGTCATCATAGCAGAC
GGGCATATACCTGATATTATCCTTAAACTGGCCAGCGGGGAAACCGAAGGTACCCGCTTTATGCCTTCACTCCACAAACC
GGACAGCCGCCAGCGCTGGATGATGAGCGGGCTTTGCACCCGCGGCAATATCTGCATAGATGACGGGGCGGTCAAAGCTA
TACGGGAAAACCAGAAAAGCCTGCTTGCCGCCGGGGTACAACAATCCGAGGGCAAATTCGGGCGGGGGGATATTGTAAAA
CTGAGCGACAGCCGCGGCAAACGGCTGGGCTATGGCATAACCAATTATTCTTCAGATGATGTTTCAAAGATAAAAGGGCT
GCATACAGACGAAATAAACACAGTTCTGGCAGGCAATCAGGGCCCGGAAATAATTCACCGCAATAACCTGGTGGTAATTT
AA

Upstream 100 bases:

>100_bases
AAAATTGTATCATCGGGAAGAGCCTTCCCTCAAGTCTGGCGGCAACTTATACGGGGGAATTCTTGTTCTGGCTTAACTTG
CTTGCTATAATGACTTATTT

Downstream 100 bases:

>100_bases
CGGAGGTAGGTCATGGCACTGGGCAGTATATACCTGGGAGACCAACTGATAGGGGAGGTAGAGTACACCCTTCAGGATAG
TTCTGATTCCCGCTGGTGGG

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MNGNCYKRIVIKLGTSLLTGGTGKLDHERMADLCRQIADLTRLGTEVIIVSSGAIAAGRAKMGLRHIPKDVPFKQVLAAI
GQSQLMNYYDQLFSPHGLTVAQGLLTKSDLSDRSGYLNARNTLLALMELGVITIVNENDVVAVDEIQQAKFGDNDNLSAM
VANLIEADLLLILTNIRGLYTADPTLHPDARLITEVKEITEELEKLAAGSSNKLGTGGMVTKLEAARLATSSGVNVIIAD
GHIPDIILKLASGETEGTRFMPSLHKPDSRQRWMMSGLCTRGNICIDDGAVKAIRENQKSLLAAGVQQSEGKFGRGDIVK
LSDSRGKRLGYGITNYSSDDVSKIKGLHTDEINTVLAGNQGPEIIHRNNLVVI

Sequences:

>Translated_373_residues
MNGNCYKRIVIKLGTSLLTGGTGKLDHERMADLCRQIADLTRLGTEVIIVSSGAIAAGRAKMGLRHIPKDVPFKQVLAAI
GQSQLMNYYDQLFSPHGLTVAQGLLTKSDLSDRSGYLNARNTLLALMELGVITIVNENDVVAVDEIQQAKFGDNDNLSAM
VANLIEADLLLILTNIRGLYTADPTLHPDARLITEVKEITEELEKLAAGSSNKLGTGGMVTKLEAARLATSSGVNVIIAD
GHIPDIILKLASGETEGTRFMPSLHKPDSRQRWMMSGLCTRGNICIDDGAVKAIRENQKSLLAAGVQQSEGKFGRGDIVK
LSDSRGKRLGYGITNYSSDDVSKIKGLHTDEINTVLAGNQGPEIIHRNNLVVI
>Mature_373_residues
MNGNCYKRIVIKLGTSLLTGGTGKLDHERMADLCRQIADLTRLGTEVIIVSSGAIAAGRAKMGLRHIPKDVPFKQVLAAI
GQSQLMNYYDQLFSPHGLTVAQGLLTKSDLSDRSGYLNARNTLLALMELGVITIVNENDVVAVDEIQQAKFGDNDNLSAM
VANLIEADLLLILTNIRGLYTADPTLHPDARLITEVKEITEELEKLAAGSSNKLGTGGMVTKLEAARLATSSGVNVIIAD
GHIPDIILKLASGETEGTRFMPSLHKPDSRQRWMMSGLCTRGNICIDDGAVKAIRENQKSLLAAGVQQSEGKFGRGDIVK
LSDSRGKRLGYGITNYSSDDVSKIKGLHTDEINTVLAGNQGPEIIHRNNLVVI

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain

Homologues:

Organism=Homo sapiens, GI21361368, Length=286, Percent_Identity=30.0699300699301, Blast_Score=115, Evalue=5e-26,
Organism=Homo sapiens, GI62912457, Length=284, Percent_Identity=30.2816901408451, Blast_Score=115, Evalue=6e-26,
Organism=Escherichia coli, GI1786437, Length=365, Percent_Identity=43.2876712328767, Blast_Score=304, Evalue=7e-84,
Organism=Caenorhabditis elegans, GI17569969, Length=267, Percent_Identity=37.8277153558052, Blast_Score=150, Evalue=8e-37,
Organism=Caenorhabditis elegans, GI17569967, Length=267, Percent_Identity=37.8277153558052, Blast_Score=150, Evalue=8e-37,
Organism=Saccharomyces cerevisiae, GI6320506, Length=415, Percent_Identity=37.1084337349398, Blast_Score=218, Evalue=2e-57,
Organism=Saccharomyces cerevisiae, GI6321822, Length=416, Percent_Identity=35.5769230769231, Blast_Score=209, Evalue=8e-55,
Organism=Drosophila melanogaster, GI21357643, Length=283, Percent_Identity=32.5088339222615, Blast_Score=126, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROB_DEHE1 (Q3Z705)

Other databases:

- EMBL:   CP000027
- RefSeq:   YP_181994.1
- ProteinModelPortal:   Q3Z705
- SMR:   Q3Z705
- STRING:   Q3Z705
- GeneID:   3229393
- GenomeReviews:   CP000027_GR
- KEGG:   det:DET1282
- NMPDR:   fig|243164.3.peg.1274
- TIGR:   DET1282
- eggNOG:   COG0263
- HOGENOM:   HBG507643
- OMA:   TFGDNDM
- PhylomeDB:   Q3Z705
- ProtClustDB:   PRK05429
- BioCyc:   DETH243164:DET_1282-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00456
- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947
- Gene3D:   G3DSA:3.40.1160.10
- PIRSF:   PIRSF000729
- PRINTS:   PR00474
- SMART:   SM00359
- TIGRFAMs:   TIGR01027

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA; SSF53633 Aa_kinase; SSF88697 PUA-like

EC number: =2.7.2.11

Molecular weight: Translated: 40167; Mature: 40167

Theoretical pI: Translated: 7.24; Mature: 7.24

Prosite motif: PS00902 GLUTAMATE_5_KINASE; PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGNCYKRIVIKLGTSLLTGGTGKLDHERMADLCRQIADLTRLGTEVIIVSSGAIAAGRA
CCCCHHHHHHHHHCCHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHH
KMGLRHIPKDVPFKQVLAAIGQSQLMNYYDQLFSPHGLTVAQGLLTKSDLSDRSGYLNAR
HHHHHHCCCCCCHHHHHHHHCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCHHH
NTLLALMELGVITIVNENDVVAVDEIQQAKFGDNDNLSAMVANLIEADLLLILTNIRGLY
HHHHHHHHCCEEEEECCCCEEEHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEE
TADPTLHPDARLITEVKEITEELEKLAAGSSNKLGTGGMVTKLEAARLATSSGVNVIIAD
ECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEC
GHIPDIILKLASGETEGTRFMPSLHKPDSRQRWMMSGLCTRGNICIDDGAVKAIRENQKS
CCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHH
LLAAGVQQSEGKFGRGDIVKLSDSRGKRLGYGITNYSSDDVSKIKGLHTDEINTVLAGNQ
HHHHCCHHCCCCCCCCCEEEECCCCCCEECCCCCCCCCCHHHHHCCCCCCCCCEEEECCC
GPEIIHRNNLVVI
CCCEEECCCEEEC
>Mature Secondary Structure
MNGNCYKRIVIKLGTSLLTGGTGKLDHERMADLCRQIADLTRLGTEVIIVSSGAIAAGRA
CCCCHHHHHHHHHCCHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHH
KMGLRHIPKDVPFKQVLAAIGQSQLMNYYDQLFSPHGLTVAQGLLTKSDLSDRSGYLNAR
HHHHHHCCCCCCHHHHHHHHCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCHHH
NTLLALMELGVITIVNENDVVAVDEIQQAKFGDNDNLSAMVANLIEADLLLILTNIRGLY
HHHHHHHHCCEEEEECCCCEEEHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEE
TADPTLHPDARLITEVKEITEELEKLAAGSSNKLGTGGMVTKLEAARLATSSGVNVIIAD
ECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEC
GHIPDIILKLASGETEGTRFMPSLHKPDSRQRWMMSGLCTRGNICIDDGAVKAIRENQKS
CCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHH
LLAAGVQQSEGKFGRGDIVKLSDSRGKRLGYGITNYSSDDVSKIKGLHTDEINTVLAGNQ
HHHHCCHHCCCCCCCCCEEEECCCCCCEECCCCCCCCCCHHHHHCCCCCCCCCEEEECCC
GPEIIHRNNLVVI
CCCEEECCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA