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The map label for this gene is trpE [H]

Identifier: 57233826

GI number: 57233826

Start: 1339193

End: 1340650

Strand: Direct

Name: trpE [H]

Synonym: DET1481

Alternate gene names: 57233826

Gene position: 1339193-1340650 (Clockwise)

Preceding gene: 57233834

Following gene: 57233825

Centisome position: 91.12

GC content: 49.66

Gene sequence:

>1458_bases
ATGTATTACCCATCTTTAGCCGAAGTAAAAAAACTGGCCGCACAGGGCAACCTGATACCCATCTCCTGTGAGATTATGGC
CGACCTTGAAACCCCGGTTTCCGCTTTTCTGAAAATCAAAGACAGCCAAAATGCTTTTCTGCTGGAAAGTGTGGAAGGCG
GGGAGCGCGTAGCCCGATATAGTTTCATCGGCACCAACCCCCATAAAGTGCTTACAGCCTATCAGACAGATACCGTTCCC
CCTCTAACCCAAGTTGAAAATGAACTAAACAAATACCGGGTAGTACCGGTGGGGGATTTGCCCCGTTTCTGCGGCGGGGC
GGTGGGTTTTCTGGGCTACGAGGCAGTTACCCGCTTTGAGGAGTTGCCCTCGCCATCGGCTGACCCCCTAAATCTTCCGG
AAGCAGTCTTCATGCTGGTTGATACCATGCTGGTTTTTGACCACATCAGCCATTCCATAAAAGTACTAAGCTATGTCCAT
ACCGAACAGGATATTGAAACGTCATATAATCAGGCTATCCGGAATATAGAAAATCTGGTTAACCGCCTTAGAAAGCCGCT
GCCCGAAACCGCCCCAAAATCTACCGCCGCAAGTATCCCCGAAATGAAATCCAATTTCAAACAGGCGGATTTTGAGGGCA
AGGTATCCAAAATAAGAGATTACCTTAACTCAGGCGAAGCTATTCAGGTAGTTTTGTCACAGCGTCTGTCCAGACCCACC
TCCGCCCATCCCTTTGACATCTACCGTGCCTTGCGTTCGGTAAACCCTTCACCATACATGTACTATCTGGATTTCGGTGA
TTTCCAGATTGTGGGCGCCTCGCCGGAGGTACTGGTACGGGTGGAAGACGGCGAGGTTATGACCCGCCCTCTGGCAGGCA
CCAGAAAACGGGGCAAAACCCAGAAAGAAGACACCAGTCTTGAGCAGGAACTCCGCCATGACGAAAAAGAGTGTGCCGAA
CATATCATGCTGGTGGATTTGGGACGAAACGATATCGGGCGTATAAGCCAGCCGGGCACAGTCCGCATAACCGACGTCAT
GGATGTGGAACGCTATTCCCACGTAATGCATCTGGTTTCCCACGTACAGGGCAAATTAAAACCAAACATTACTCCGTTTG
AGGCTTTGCAATCCTGCTTCCCGGCAGGCACAGTCTCAGGCGCACCTAAAATACGAGCTATGGAAATAATAGCTGAAATG
GAAACCGAAAAGAGAGGCATTTATGCCGGGGCAGTCGGATATTTTTCTTATTCGGGCAATATGGACATGGCTATAGCCAT
ACGCACCATGGTTGTCAAGGGAGGCATTGCCCATATCCAGGCAGGCTGCGGCATAGTAAGTGACAGCGTACCCGAACATG
AGTATCAGGAAACATTAAACAAAGCTCAGGCTTTGCTGAAAGCTCTGGACAGGGCAGAAAATCAGGCATCGGAGAAACCG
CATGTTATTACTAATTGA

Upstream 100 bases:

>100_bases
CTGTATTTTTTGCAGGGATAATTAGGGTGGCACCGCGAAGGTTAATCCCTCGCCCCTAAAAAGGACGAGGGATTTATTTT
TTTGCGAGGTGCCCGTTAAA

Downstream 100 bases:

>100_bases
TAATTACGACAGTTTTACCTATAACCTTTTTCAGTATTTTTCCGAACTGGGGCAGGAGGTTAAGGTAGTCCGCAATAACA
AGATTACCCTTGATGCCATA

Product: anthranilate synthase component I

Products: NA

Alternate protein names: Anthranilate synthase component I [H]

Number of amino acids: Translated: 485; Mature: 485

Protein sequence:

>485_residues
MYYPSLAEVKKLAAQGNLIPISCEIMADLETPVSAFLKIKDSQNAFLLESVEGGERVARYSFIGTNPHKVLTAYQTDTVP
PLTQVENELNKYRVVPVGDLPRFCGGAVGFLGYEAVTRFEELPSPSADPLNLPEAVFMLVDTMLVFDHISHSIKVLSYVH
TEQDIETSYNQAIRNIENLVNRLRKPLPETAPKSTAASIPEMKSNFKQADFEGKVSKIRDYLNSGEAIQVVLSQRLSRPT
SAHPFDIYRALRSVNPSPYMYYLDFGDFQIVGASPEVLVRVEDGEVMTRPLAGTRKRGKTQKEDTSLEQELRHDEKECAE
HIMLVDLGRNDIGRISQPGTVRITDVMDVERYSHVMHLVSHVQGKLKPNITPFEALQSCFPAGTVSGAPKIRAMEIIAEM
ETEKRGIYAGAVGYFSYSGNMDMAIAIRTMVVKGGIAHIQAGCGIVSDSVPEHEYQETLNKAQALLKALDRAENQASEKP
HVITN

Sequences:

>Translated_485_residues
MYYPSLAEVKKLAAQGNLIPISCEIMADLETPVSAFLKIKDSQNAFLLESVEGGERVARYSFIGTNPHKVLTAYQTDTVP
PLTQVENELNKYRVVPVGDLPRFCGGAVGFLGYEAVTRFEELPSPSADPLNLPEAVFMLVDTMLVFDHISHSIKVLSYVH
TEQDIETSYNQAIRNIENLVNRLRKPLPETAPKSTAASIPEMKSNFKQADFEGKVSKIRDYLNSGEAIQVVLSQRLSRPT
SAHPFDIYRALRSVNPSPYMYYLDFGDFQIVGASPEVLVRVEDGEVMTRPLAGTRKRGKTQKEDTSLEQELRHDEKECAE
HIMLVDLGRNDIGRISQPGTVRITDVMDVERYSHVMHLVSHVQGKLKPNITPFEALQSCFPAGTVSGAPKIRAMEIIAEM
ETEKRGIYAGAVGYFSYSGNMDMAIAIRTMVVKGGIAHIQAGCGIVSDSVPEHEYQETLNKAQALLKALDRAENQASEKP
HVITN
>Mature_485_residues
MYYPSLAEVKKLAAQGNLIPISCEIMADLETPVSAFLKIKDSQNAFLLESVEGGERVARYSFIGTNPHKVLTAYQTDTVP
PLTQVENELNKYRVVPVGDLPRFCGGAVGFLGYEAVTRFEELPSPSADPLNLPEAVFMLVDTMLVFDHISHSIKVLSYVH
TEQDIETSYNQAIRNIENLVNRLRKPLPETAPKSTAASIPEMKSNFKQADFEGKVSKIRDYLNSGEAIQVVLSQRLSRPT
SAHPFDIYRALRSVNPSPYMYYLDFGDFQIVGASPEVLVRVEDGEVMTRPLAGTRKRGKTQKEDTSLEQELRHDEKECAE
HIMLVDLGRNDIGRISQPGTVRITDVMDVERYSHVMHLVSHVQGKLKPNITPFEALQSCFPAGTVSGAPKIRAMEIIAEM
ETEKRGIYAGAVGYFSYSGNMDMAIAIRTMVVKGGIAHIQAGCGIVSDSVPEHEYQETLNKAQALLKALDRAENQASEKP
HVITN

Specific function: Tryptophan biosynthesis; first step. [C]

COG id: COG0147

COG function: function code EH; Anthranilate/para-aminobenzoate synthases component I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anthranilate synthase component I family [H]

Homologues:

Organism=Escherichia coli, GI1787518, Length=376, Percent_Identity=40.9574468085106, Blast_Score=287, Evalue=1e-78,
Organism=Escherichia coli, GI1788114, Length=443, Percent_Identity=32.7313769751693, Blast_Score=247, Evalue=2e-66,
Organism=Escherichia coli, GI87082077, Length=219, Percent_Identity=32.4200913242009, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1786809, Length=282, Percent_Identity=26.9503546099291, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6320935, Length=492, Percent_Identity=43.9024390243902, Blast_Score=390, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6324361, Length=415, Percent_Identity=24.8192771084337, Blast_Score=120, Evalue=6e-28,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR019999
- InterPro:   IPR006805
- InterPro:   IPR005256
- InterPro:   IPR015890 [H]

Pfam domain/function: PF04715 Anth_synt_I_N; PF00425 Chorismate_bind [H]

EC number: =4.1.3.27 [H]

Molecular weight: Translated: 53788; Mature: 53788

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYYPSLAEVKKLAAQGNLIPISCEIMADLETPVSAFLKIKDSQNAFLLESVEGGERVARY
CCCCCHHHHHHHHHCCCEEEEEEEEEECCCCCHHHHEEECCCCCEEEEEECCCCHHEEEE
SFIGTNPHKVLTAYQTDTVPPLTQVENELNKYRVVPVGDLPRFCGGAVGFLGYEAVTRFE
EEECCCHHHEEEEECCCCCCCHHHHHHHHHCEEEEECCCCHHHHHHHHHHHHHHHHHHHH
ELPSPSADPLNLPEAVFMLVDTMLVFDHISHSIKVLSYVHTEQDIETSYNQAIRNIENLV
HCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NRLRKPLPETAPKSTAASIPEMKSNFKQADFEGKVSKIRDYLNSGEAIQVVLSQRLSRPT
HHHHHCCCCCCCCCHHHCCHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCC
SAHPFDIYRALRSVNPSPYMYYLDFGDFQIVGASPEVLVRVEDGEVMTRPLAGTRKRGKT
CCCHHHHHHHHHCCCCCCEEEEEECCCEEEECCCCEEEEEECCCCEEECCCCCCHHCCCC
QKEDTSLEQELRHDEKECAEHIMLVDLGRNDIGRISQPGTVRITDVMDVERYSHVMHLVS
CCHHHHHHHHHHCCHHHHHHHEEEEECCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHH
HVQGKLKPNITPFEALQSCFPAGTVSGAPKIRAMEIIAEMETEKRGIYAGAVGYFSYSGN
HHHCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEEEEEEECCC
MDMAIAIRTMVVKGGIAHIQAGCGIVSDSVPEHEYQETLNKAQALLKALDRAENQASEKP
CCHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
HVITN
CCCCC
>Mature Secondary Structure
MYYPSLAEVKKLAAQGNLIPISCEIMADLETPVSAFLKIKDSQNAFLLESVEGGERVARY
CCCCCHHHHHHHHHCCCEEEEEEEEEECCCCCHHHHEEECCCCCEEEEEECCCCHHEEEE
SFIGTNPHKVLTAYQTDTVPPLTQVENELNKYRVVPVGDLPRFCGGAVGFLGYEAVTRFE
EEECCCHHHEEEEECCCCCCCHHHHHHHHHCEEEEECCCCHHHHHHHHHHHHHHHHHHHH
ELPSPSADPLNLPEAVFMLVDTMLVFDHISHSIKVLSYVHTEQDIETSYNQAIRNIENLV
HCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NRLRKPLPETAPKSTAASIPEMKSNFKQADFEGKVSKIRDYLNSGEAIQVVLSQRLSRPT
HHHHHCCCCCCCCCHHHCCHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCC
SAHPFDIYRALRSVNPSPYMYYLDFGDFQIVGASPEVLVRVEDGEVMTRPLAGTRKRGKT
CCCHHHHHHHHHCCCCCCEEEEEECCCEEEECCCCEEEEEECCCCEEECCCCCCHHCCCC
QKEDTSLEQELRHDEKECAEHIMLVDLGRNDIGRISQPGTVRITDVMDVERYSHVMHLVS
CCHHHHHHHHHHCCHHHHHHHEEEEECCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHH
HVQGKLKPNITPFEALQSCFPAGTVSGAPKIRAMEIIAEMETEKRGIYAGAVGYFSYSGN
HHHCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEEEEEEECCC
MDMAIAIRTMVVKGGIAHIQAGCGIVSDSVPEHEYQETLNKAQALLKALDRAENQASEKP
CCHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
HVITN
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2732211 [H]